(Linear) (Six-base) MAPSORT of: KIEE3689.seq Check: 8988 from: 1 to: 2055 KIEE3689.orf With 182 enzymes: SgfI * March 22, 2012 16:39 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 28 163 899 910 1592 2055 Size: 28 135 736 11 682 463 Fragments arranged by size: 736 682 463 135 28 11 AfeI AGC'GCT Cuts at: 0 1474 2055 Size: 1474 581 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2033 2055 Size: 2033 22 ApoI r'AATT_y Cuts at: 0 401 884 913 1878 2055 Size: 401 483 29 965 177 Fragments arranged by size: 965 483 401 177 29 AseI AT'TA_AT Cuts at: 0 1099 2055 Size: 1099 956 AvaI C'yCGr_G Cuts at: 0 187 961 2055 Size: 187 774 1094 Fragments arranged by size: 1094 774 187 BamHI G'GATC_C Cuts at: 0 114 2055 Size: 114 1941 BanII G_rGCy'C Cuts at: 0 248 2055 Size: 248 1807 BbsI GAAGACnn'nnnn_ Cuts at: 0 151 341 1457 2055 Size: 151 190 1116 598 Fragments arranged by size: 1116 598 190 151 BbvCI CC'TCA_GC Cuts at: 0 1718 2055 Size: 1718 337 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 263 297 1955 1989 2055 Size: 263 34 1658 34 66 Fragments arranged by size: 1658 263 66 34 34 BclI T'GATC_A Cuts at: 0 690 2055 Size: 690 1365 BlpI GC'TnA_GC Cuts at: 0 127 1476 2055 Size: 127 1349 579 Fragments arranged by size: 1349 579 127 BmrI ACTGGGnnnn_n' Cuts at: 0 417 2055 Size: 417 1638 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1676 2055 Size: 1676 379 Bpu10I CC'TnA_GC Cuts at: 0 1718 2055 Size: 1718 337 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 244 1580 2055 Size: 244 1336 475 Fragments arranged by size: 1336 475 244 BsaI GGTCTCn'nnnn_ Cuts at: 0 1598 2055 Size: 1598 457 BsaAI yAC'GTr Cuts at: 0 448 2055 Size: 448 1607 BsaWI w'CCGG_w Cuts at: 0 117 2055 Size: 117 1938 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 16 2055 Size: 16 2039 BseYI C'CCAG_C Cuts at: 0 104 500 738 1248 1957 2055 Size: 104 396 238 510 709 98 Fragments arranged by size: 709 510 396 238 104 98 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 737 1831 2055 Size: 737 1094 224 Fragments arranged by size: 1094 737 224 BsiHKAI G_wGCw'C Cuts at: 0 248 602 2055 Size: 248 354 1453 Fragments arranged by size: 1453 354 248 Bsp1286I G_dGCh'C Cuts at: 0 248 602 2055 Size: 248 354 1453 Fragments arranged by size: 1453 354 248 BsrFI r'CCGG_y Cuts at: 0 1985 2055 Size: 1985 70 BstXI CCAn_nnnn'nTGG Cuts at: 0 108 2055 Size: 108 1947 BstYI r'GATC_y Cuts at: 0 114 2055 Size: 114 1941 BtgI C'CryG_G Cuts at: 0 932 2055 Size: 932 1123 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 285 320 2055 Size: 285 35 1735 Fragments arranged by size: 1735 285 35 DraI TTT'AAA Cuts at: 0 609 1551 2055 Size: 609 942 504 Fragments arranged by size: 942 609 504 EaeI y'GGCC_r Cuts at: 0 53 2055 Size: 53 2002 EarI CTCTTCn'nnn_ Cuts at: 0 489 771 1245 1303 1402 1568 2055 Size: 489 282 474 58 99 166 487 Fragments arranged by size: 489 487 474 282 166 99 58 EcoICRI GAG'CTC Cuts at: 0 246 2055 Size: 246 1809 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 28 163 899 910 1592 1676 2055 Size: 28 135 736 11 682 84 379 Fragments arranged by size: 736 682 379 135 84 28 11 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 580 2055 Size: 580 1475 HaeII r_GCGC'y Cuts at: 0 127 1476 2055 Size: 127 1349 579 Fragments arranged by size: 1349 579 127 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 488 500 520 532 606 638 1567 1599 Size: 488 12 20 12 74 32 929 32 Cuts at: 1599 2035 2055 Size: 436 20 Fragments arranged by size: 929 488 436 74 32 32 20 20 12 12 HincII GTy'rAC Cuts at: 0 1826 2055 Size: 1826 229 HindIII A'AGCT_T Cuts at: 0 10 2055 Size: 10 2045 HpaI GTT'AAC Cuts at: 0 1826 2055 Size: 1826 229 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1565 2055 Size: 1565 490 MscI TGG'CCA Cuts at: 0 55 2055 Size: 55 2000 MslI CAynn'nnrTG Cuts at: 0 320 1712 1945 2055 Size: 320 1392 233 110 Fragments arranged by size: 1392 320 233 110 MspA1I CmG'CkG Cuts at: 0 108 2055 Size: 108 1947 NcoI C'CATG_G Cuts at: 0 932 2055 Size: 932 1123 NdeI CA'TA_TG Cuts at: 0 1782 2016 2055 Size: 1782 234 39 Fragments arranged by size: 1782 234 39 PflMI CCAn_nnn'nTGG Cuts at: 0 77 111 2055 Size: 77 34 1944 Fragments arranged by size: 1944 77 34 PfoI T'CCnGG_A Cuts at: 0 203 1692 2055 Size: 203 1489 363 Fragments arranged by size: 1489 363 203 PshAI GACnn'nnGTC Cuts at: 0 383 2055 Size: 383 1672 PsiI TTA'TAA Cuts at: 0 653 2000 2055 Size: 653 1347 55 Fragments arranged by size: 1347 653 55 PvuII CAG'CTG Cuts at: 0 108 2055 Size: 108 1947 SacI G_AGCT'C Cuts at: 0 248 2055 Size: 248 1807 ScaI AGT'ACT Cuts at: 0 543 2055 Size: 543 1512 SfcI C'TryA_G Cuts at: 0 1430 2055 Size: 1430 625 SmlI C'TyrA_G Cuts at: 0 259 1595 2055 Size: 259 1336 460 Fragments arranged by size: 1336 460 259 SnaBI TAC'GTA Cuts at: 0 448 2055 Size: 448 1607 SspI AAT'ATT Cuts at: 0 1744 2055 Size: 1744 311 StyI C'CwwG_G Cuts at: 0 389 932 1272 2055 Size: 389 543 340 783 Fragments arranged by size: 783 543 389 340 SwaI ATTT'AAAT Cuts at: 0 1551 2055 Size: 1551 504 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 118 1262 2055 Size: 118 1144 793 Fragments arranged by size: 1144 793 118 TatI w'GTAC_w Cuts at: 0 541 1264 1852 2055 Size: 541 723 588 203 Fragments arranged by size: 723 588 541 203 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 672 704 2055 Size: 672 32 1351 Fragments arranged by size: 1351 672 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 108 2055 Size: 108 1947 Enzymes that do cut: AcuI AfeI AlfI ApoI AseI AvaI BamHI BanII BbsI BbvCI BcgI BclI BlpI BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaWI BseRI BseYI BsgI BsiHKAI Bsp1286I BsrFI BstXI BstYI BtgI CspCI DraI EaeI EarI EcoICRI Eco57MI FalI HaeII Hin4I HincII HindIII HpaI MmeI MscI MslI MspA1I NcoI NdeI PflMI PfoI PshAI PsiI PvuII SacI ScaI SfcI SmlI SnaBI SspI StyI SwaI TaqII TatI TstI XcmI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AflII AflIII AgeI AhdI AleI AloI AlwNI ApaI ApaLI AscI AsiSI AvrII BaeI BanI BbeI BciVI BfrBI BglI BglII Bme1580I BmgBI BmtI BplI BsaBI BsaHI BsaXI BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI BtsI ClaI DraIII DrdI EagI EciI EcoNI EcoO109I EcoRI EcoRV FseI FspI FspAI KasI KpnI MfeI MluI NaeI NarI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PmeI PmlI PpiI PpuMI PspOMI PspXI PsrI PstI PvuI RsrII SacII SalI SanDI SapI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SpeI SphI SrfI StuI Tth111I XbaI XhoI XmaI XmnI ZraI