################################## # NO CUT ENZYMES ( 162 enzymes) # ################################## AatII AccII AccIII AclI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AspEI AspI AvaI AviII AvrII BalI BamHI BbeI BbrPI BbvCI BbvI BcgI BciVI BcnI BfrI BglII BlnI BlpI Bpu1102I BsaI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstPI BstUI BstXI BstYI Bsu36I CelII CfoI Cfr10I ClaI CpoI DraII DraIII DrdI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I EcoRI EcoRV FauI Fnu4HI FseI FspI HaeII HapII HhaI HinP1I HincII HindII HindIII HpaI HpaII HphI ItaI KasI Ksp632I KspI MfeI MflI MluI MluNI MroI MscI MspA1I MspI MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NspI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PstI PvuI PvuII RsrII SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SpeI SphI Sse8387I SspBI StuI SwaI TseI Tth111I XbaI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### Acc65I 494 GGTACC G^GTACC AccI 713 GTATAC GT^[AC][GT]AC AciI 934 CCGC CCGC(-3/-1) AcyI 917 GACGCC G[GA]^CG[CT]C AlwI 121 GATCC GGATC(4/5) AlwNI 592 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG AseI 657 ATTAAT AT^TAAT AsnI 657 ATTAAT AT^TAAT Asp700I 388 GAAAATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 494 GGTACC G^GTACC AvaII 621 GGACC G^G[AT]CC BanI 494 GGTACC G^G[CT][GA]CC BfaI 649 CTAG C^TAG BglI 594 GCCACTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaBI 760 GATGTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 917 GACGCC G[GA]^CG[CT]C BsaJI 702 CCATGG C^C[ACGT][ACGT]GG BspHI 785 TCATGA T^CATGA BsrBI 934 CCGCTC CCGCTC(-3/-3) BsrI 28 ACTGG ACTGG(1/-1) Bst1107I 713 GTATAC GTA^TAC BstZ17I 713 GTATAC GTA^TAC Cac8I 590 GCCAGC GC[ACGT]^[ACGT]GC DsaI 702 CCATGG C^C[GA][CT]GG EcoNI 195 CCTCATCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 702 CCATGG C^C[AT][AT]GG EcoT22I 226 ATGCAT ATGCA^T HgaI 917 GACGC GACGC(5/10) Hin1I 917 GACGCC G[GA]^CG[CT]C KpnI 494 GGTACC GGTAC^C MaeI 649 CTAG C^TAG MamI 760 GATGTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MslI 198 CATCGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MwoI 594 GCCACTGTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NcoI 702 CCATGG C^CATGG NlaIV 494 GGTACC GG[ACGT]^[ACGT]CC NsiI 226 ATGCAT ATGCA^T PflMI 834 CCAACTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshBI 657 ATTAAT AT^TAAT RcaI 785 TCATGA T^CATGA SmlI 742 CTTGAG C^T[CT][GA]AG SnaBI 155 TACGTA TAC^GTA SspI 391 AATATT AAT^ATT StyI 702 CCATGG C^C[AT][AT]GG Tsp45I 428 GTGAC ^GT[GC]AC TspRI 596 CACTG CA[GC]TG[ACGT][ACGT]^ Van91I 834 CCAACTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmnI 388 GAAAATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AcsI 100 AAATTT [GA]^AATT[CT] 446 AAATTC AfaI 495 GTAC GT^AC 928 GTAC ApoI 100 AAATTT [GA]^AATT[CT] 446 AAATTC BbsI 727 GAAGAC GAAGAC(2/6) 914 GAAGAC BclI 249 TGATCA T^GATCA 305 TGATCA BpmI 29 CTGGAG CTGGAG(16/14) 841 CTGGAG BpuAI 727 GAAGAC GAAGAC(2/6) 914 GAAGAC BsaAI 155 TACGTA [CT]AC^GT[GA] 893 CACGTA BsiYI 195 CCTCATCGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 834 CCAACTCCTGG BslI 195 CCTCATCGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 834 CCAACTCCTGG BstF5I 178 CATCC GGATG(2/0) 766 CATCC Cfr13I 621 GGACC G^G[ACGT]CC 873 GGCCC DraI 261 TTTAAA TTT^AAA 682 TTTAAA FbaI 249 TGATCA T^GATCA 305 TGATCA FokI 178 CATCC GGATG(9/13) 766 CATCC MaeII 156 ACGT A^CGT 894 ACGT MaeIII 428 GTGAC ^GT[ACGT]AC 689 GTAAC RsaI 495 GTAC GT^AC 928 GTAC Sau96I 621 GGACC G^G[ACGT]CC 873 GGCCC SfaNI 463 GATGC GCATC(5/9) 707 GCATC TaqI 200 TCGA T^CGA 545 TCGA TthHB8I 200 TCGA T^CGA 545 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 26 enzymes) # ######################################### AluI AspHI BanII BmyI BsiHKAI Bsp1286I BssKI BstNI DdeI DpnII EcoRII HaeIII HinfI MboI MboII MnlI MseI MvaI NdeII NlaIII SacI Sau3AI ScrFI TfiI Tru9I Tsp509I