(Circular) (Six-base) MAPSORT of: pFN21AE3411.seq Check: 1161 from: 1 to: 5650 pFN21AE3411 5650 bp With 182 enzymes: SgfI * March 28, 2012 12:53 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3460 278 Size: 53 83 186 936 1924 2468 Fragments arranged by size: 2468 1924 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2719 2993 1937 Size: 782 274 4594 Fragments arranged by size: 4594 782 274 Acc65I G'GTAC_C Cuts at: 2499 2971 2499 Size: 472 5178 AclI AA'CG_TT Cuts at: 1563 3777 4150 1563 Size: 2214 373 3063 Fragments arranged by size: 3063 2214 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2139 2529 2825 3713 5097 5365 1632 Size: 507 390 296 888 1384 268 1917 Fragments arranged by size: 1917 1384 888 507 390 296 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5453 AflIII A'CryG_T Cuts at: 1203 4549 5526 1203 Size: 3346 977 1327 Fragments arranged by size: 3346 1327 977 AgeI A'CCGG_T Cuts at: 4481 4481 Size: 5650 AhdI GACnn_n'nnGTC Cuts at: 4379 4379 Size: 5650 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5650 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5650 AlwNI CAG_nnn'CTG Cuts at: 2602 3393 4965 2602 Size: 791 1572 3287 Fragments arranged by size: 3287 1572 791 ApaLI G'TGCA_C Cuts at: 3707 4863 3707 Size: 1156 4494 ApoI r'AATT_y Cuts at: 1493 2105 2451 2959 3185 3337 5446 1493 Size: 612 346 508 226 152 2109 1697 Fragments arranged by size: 2109 1697 612 508 346 226 152 AseI AT'TA_AT Cuts at: 160 1032 2663 4204 160 Size: 872 1631 1541 1606 Fragments arranged by size: 1631 1606 1541 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5650 AvaI C'yCGr_G Cuts at: 1870 1943 2975 1870 Size: 73 1032 4545 Fragments arranged by size: 4545 1032 73 BamHI G'GATC_C Cuts at: 2980 3347 2980 Size: 367 5283 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2499 2971 4426 618 Size: 325 217 627 712 472 1455 1842 Fragments arranged by size: 1842 1455 712 627 472 325 217 BanII G_rGCy'C Cuts at: 1391 2509 2586 2855 2969 1391 Size: 1118 77 269 114 4072 Fragments arranged by size: 4072 1118 269 114 77 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2739 2926 928 Size: 971 840 187 3652 Fragments arranged by size: 3652 971 840 187 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3841 3875 1903 Size: 34 1904 34 3678 Fragments arranged by size: 3678 1904 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3542 4752 3542 Size: 1210 4440 BclI T'GATC_A Cuts at: 1548 2254 2310 1548 Size: 706 56 4888 Fragments arranged by size: 4888 706 56 BfrBI ATG'CAT Cuts at: 2233 2233 Size: 5650 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2605 4261 136 Size: 107 122 71 631 605 933 1656 1525 Fragments arranged by size: 1656 1525 933 631 605 122 107 71 BglII A'GATC_T Cuts at: 5645 5645 Size: 5650 BlpI GC'TnA_GC Cuts at: 3067 3067 Size: 5650 Bme1580I G_kGCm'C Cuts at: 1790 3711 4867 1790 Size: 1921 1156 2573 Fragments arranged by size: 2573 1921 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5650 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3381 4339 442 Size: 416 53 83 399 24 1964 958 1753 Fragments arranged by size: 1964 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5650 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2055 2867 4310 1239 Size: 816 812 1443 2579 Fragments arranged by size: 2579 1443 816 812 Bpu10I CC'TnA_GC Cuts at: 2724 2724 Size: 5650 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2768 3775 4640 4938 2768 Size: 1007 865 298 3480 Fragments arranged by size: 3480 1007 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4313 882 Size: 617 2814 2219 Fragments arranged by size: 2814 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2162 2900 3441 493 Size: 711 958 738 541 2702 Fragments arranged by size: 2702 958 738 711 541 BsaBI GATnn'nnATC Cuts at: 2769 5644 2769 Size: 2875 2775 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2923 3457 3839 4474 Size: 53 83 186 936 1390 534 382 635 Cuts at: 4474 275 Size: 1451 Fragments arranged by size: 1451 1390 936 635 534 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4083 4481 4755 4902 5313 4083 Size: 398 274 147 411 4420 Fragments arranged by size: 4420 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5425 Fragments arranged by size: 5425 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5650 BseYI C'CCAG_C Cuts at: 4853 5349 4853 Size: 496 5154 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5077 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3100 3861 4889 1540 Size: 275 186 1099 761 1028 2301 Fragments arranged by size: 2301 1099 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2509 2586 2855 3711 3796 4867 2509 Size: 77 269 856 85 1071 3292 Fragments arranged by size: 3292 1071 856 269 85 77 BsmI GAATG_Cn' Cuts at: 3161 3254 3161 Size: 93 5557 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2509 2586 2855 2969 3711 3796 4867 Size: 399 719 77 269 114 742 85 1071 Cuts at: 4867 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 719 399 269 114 85 77 BspEI T'CCGG_A Cuts at: 5313 5313 Size: 5650 BspHI T'CATG_A Cuts at: 2790 3539 5269 2790 Size: 749 1730 3171 Fragments arranged by size: 3171 1730 749 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3005 844 Size: 2161 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2941 3537 1394 Size: 429 1118 596 3507 Fragments arranged by size: 3507 1118 596 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4145 4319 65 Size: 1183 2897 174 1396 Fragments arranged by size: 2897 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4294 4481 1700 Size: 110 2484 187 2869 Fragments arranged by size: 2869 2484 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5650 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5650 BssSI C'ACGA_G Cuts at: 3710 4722 3710 Size: 1012 4638 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5650 BstBI TT'CG_AA Cuts at: 4454 4454 Size: 5650 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5650 BstYI r'GATC_y Cuts at: 1972 2980 3347 3732 3749 5201 5287 5299 5645 Size: 1008 367 385 17 1452 86 12 346 Cuts at: 5645 1972 Size: 1977 Fragments arranged by size: 1977 1452 1008 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2720 2720 Size: 5650 BtgI C'CryG_G Cuts at: 513 1065 2004 2707 513 Size: 552 939 703 3456 Fragments arranged by size: 3456 939 703 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4911 Fragments arranged by size: 4911 683 56 BtsI GCAGTG_nn' Cuts at: 3166 3960 3980 3166 Size: 794 20 4836 Fragments arranged by size: 4836 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5650 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5615 DraI TTT'AAA Cuts at: 2268 2689 2954 2268 Size: 421 265 4964 Fragments arranged by size: 4964 421 265 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4657 809 Size: 507 3341 1802 Fragments arranged by size: 3341 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3097 3986 5615 Size: 54 1425 50 275 18 1267 889 1629 Cuts at: 5615 8 Size: 43 Fragments arranged by size: 1629 1425 1267 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3097 1537 Size: 275 1285 4090 Fragments arranged by size: 4090 1285 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3580 1353 Size: 2227 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4237 4611 4757 387 Size: 1064 138 2648 374 146 1280 Fragments arranged by size: 2648 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2507 2584 2853 2507 Size: 77 269 5304 Fragments arranged by size: 5304 269 77 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2055 2139 2529 2825 2867 3713 4310 Size: 393 423 84 390 296 42 846 597 Cuts at: 4310 5097 5365 1239 Size: 787 268 1524 Fragments arranged by size: 1524 846 787 597 423 393 390 296 268 84 42 EcoNI CCTnn'n_nnAGG Cuts at: 2204 2204 Size: 5650 EcoRI G'AATT_C Cuts at: 2959 3337 2959 Size: 378 5272 FspI TGC'GCA Cuts at: 4156 4156 Size: 5650 HaeII r_GCGC'y Cuts at: 1678 4797 1678 Size: 3119 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4291 4323 4365 4397 1373 Size: 32 135 32 2719 32 42 32 2626 Fragments arranged by size: 2719 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2994 3240 669 Size: 988 281 1056 246 3079 Fragments arranged by size: 3079 1056 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5650 HpaI GTT'AAC Cuts at: 3240 3240 Size: 5650 KpnI G_GTAC'C Cuts at: 2503 2975 2503 Size: 472 5178 MfeI C'AATT_G Cuts at: 3249 3249 Size: 5650 MluI A'CGCG_T Cuts at: 5526 5526 Size: 5650 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4764 4948 1305 Size: 3459 184 2007 Fragments arranged by size: 3459 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3828 Fragments arranged by size: 3828 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2207 3608 3967 4126 518 Size: 648 1041 1401 359 159 2042 Fragments arranged by size: 2042 1401 1041 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3066 3743 4891 5136 1606 Size: 1460 677 1148 245 2120 Fragments arranged by size: 2120 1460 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2707 513 Size: 552 939 703 3456 Fragments arranged by size: 3456 939 703 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5650 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5650 NotI GC'GGCC_GC Cuts at: 3097 3097 Size: 5650 NsiI A_TGCA'T Cuts at: 2235 2235 Size: 5650 NspI r_CATG'y Cuts at: 1132 1229 3008 4553 1132 Size: 97 1779 1545 2229 Fragments arranged by size: 2229 1779 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5650 PciI A'CATG_T Cuts at: 4549 4549 Size: 5650 PflMI CCAn_nnn'nTGG Cuts at: 1108 2845 1108 Size: 1737 3913 PfoI T'CCnGG_A Cuts at: 2461 2844 2461 Size: 383 5267 PmeI GTTT'AAAC Cuts at: 2954 2954 Size: 5650 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2741 2773 3719 3751 5232 5264 2741 Size: 32 946 32 1481 32 3127 Fragments arranged by size: 3127 1481 946 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5650 PsiI TTA'TAA Cuts at: 3220 3220 Size: 5650 PstI C_TGCA'G Cuts at: 830 3002 830 Size: 2172 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5650 SacI G_AGCT'C Cuts at: 2509 2586 2855 2509 Size: 77 269 5304 Fragments arranged by size: 5304 269 77 SalI G'TCGA_C Cuts at: 1936 2992 1936 Size: 1056 4594 SbfI CC_TGCA'GG Cuts at: 3002 3002 Size: 5650 ScaI AGT'ACT Cuts at: 3898 5610 3898 Size: 1712 3938 SfcI C'TryA_G Cuts at: 826 1045 2998 4133 4814 5005 826 Size: 219 1953 1135 681 191 1471 Fragments arranged by size: 1953 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5650 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5650 SmaI CCC'GGG Cuts at: 1872 2977 1872 Size: 1105 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2747 3754 4655 4917 820 Size: 197 926 804 1007 901 262 1553 Fragments arranged by size: 1553 1007 926 901 804 262 197 SnaBI TAC'GTA Cuts at: 493 2162 3441 493 Size: 1669 1279 2702 Fragments arranged by size: 2702 1669 1279 SpeI A'CTAG_T Cuts at: 152 152 Size: 5650 SphI G_CATG'C Cuts at: 1132 3008 1132 Size: 1876 3774 SspI AAT'ATT Cuts at: 5 52 2398 3574 5 Size: 47 2346 1176 2081 Fragments arranged by size: 2346 2081 1176 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5650 StyI C'CwwG_G Cuts at: 513 1065 2004 2707 3089 513 Size: 552 939 703 382 3074 Fragments arranged by size: 3074 939 703 552 382 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3677 3694 3847 4032 4477 325 Size: 1255 2097 17 153 185 445 1498 Fragments arranged by size: 2097 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3896 5608 96 Size: 274 80 33 51 1443 1919 1712 138 Fragments arranged by size: 1919 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5610 5642 5610 Size: 32 5618 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5650 XbaI T'CTAG_A Cuts at: 2986 2986 Size: 5650 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5650 XmaI C'CCGG_G Cuts at: 1870 2975 1870 Size: 1105 4545 XmnI GAAnn'nnTTC Cuts at: 2397 3779 2397 Size: 1382 4268 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3458 276 Size: 53 83 186 936 1924 2468 Fragments arranged by size: 2468 1924 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BsmBI BstXI Bsu36I DraIII EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI