################################## # NO CUT ENZYMES ( 105 enzymes) # ################################## Acc65I AccI AclI AflIII AgeI AhdI Alw44I Aor51HI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AvaI AviII AvrII BamHI BbeI BbrPI BcgI BciVI BglII BlnI BlpI Bpu1102I BsaAI BsgI BsiEI BsiHKAI BsiWI BsoBI Bsp1407I BspDI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstXI BstYI BstZ17I CelII Cfr10I ClaI CpoI DsaI EagI Eam1105I EclXI Eco47III Eco52I EcoT22I FseI FspI HgaI KasI KpnI KspI MflI MluI NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI SexAI SfiI SfoI SgrAI SmaI SnaBI SphI SspBI SwaI XbaI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AatII 1377 GACGTC GACGT^C AccIII 562 TCCGGA T^CCGGA AcyI 1377 GACGTC G[GA]^CG[CT]C ApaI 620 GGGCCC GGGCC^C AspI 257 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 698 TGGCCA TGG^CCA BanI 871 GGTGCC G^G[CT][GA]CC BglI 1501 GCCACCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaBI 3025 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 1377 GACGTC G[GA]^CG[CT]C BsaI 1181 GAGACC GGTCTC(1/5) BseAI 562 TCCGGA T^CCGGA BsmI 2552 GCATTC GAATGC(1/-1) BspEI 562 TCCGGA T^CCGGA BstBI 1222 TTCGAA TT^CGAA BstEII 1398 GGTGACC G^GT[ACGT]ACC BstPI 1398 GGTGACC G^GT[ACGT]ACC CfoI 911 GCGC GCG^C DrdI 2394 GACCCACTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 698 TGGCCA [CT]^GGCC[GA] EcoO65I 1398 GGTGACC G^GT[ACGT]ACC FauI 2833 GCGGG CCCGC(4/6) HaeII 910 AGCGCC [GA]GCGC^[CT] HhaI 911 GCGC GCG^C Hin1I 1377 GACGTC G[GA]^CG[CT]C HinP1I 911 GCGC G^CGC HincII 2979 GTTAAC GT[CT]^[GA]AC HindII 2979 GTTAAC GT[CT]^[GA]AC HpaI 2979 GTTAAC GTT^AAC MamI 3025 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 1809 CAATTG C^AATTG MluNI 698 TGGCCA TGG^CCA MroI 562 TCCGGA T^CCGGA MscI 698 TGGCCA TGG^CCA MspA1I 451 CAGCTG C[AC]G^C[GT]G MunI 1809 CAATTG C^AATTG NruI 102 TCGCGA TCG^CGA NspV 1222 TTCGAA TT^CGAA PflFI 257 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 548 CCATGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 2714 AGGTCCC [GA]G^G[AT]CC[CT] PvuII 451 CAGCTG CAG^CTG SbfI 471 CCTGCAGG CCTGCA^GG ScaI 2917 AGTACT AGT^ACT SfuI 1222 TTCGAA TT^CGAA SpeI 3060 ACTAGT A^CTAGT Sse8387I 471 CCTGCAGG CCTGCA^GG SspI 2046 AATATT AAT^ATT Tth111I 257 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 548 CCATGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AccII 103 CGCG CG^CG 442 CGCG AflII 217 CTTAAG C^TTAAG 2890 CTTAAG AlwI 2938 GATCC GGATC(4/5) 158 GGATC AlwNI 404 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG 2124 CAGCACCTG AocI 849 CCTGAGG CC^T[ACGT]AGG 2067 CCTCAGG BbvCI 2121 CCTCAGC CCTCAGC(-5/-2) 883 GCTGAGG BclI 651 TGATCA T^GATCA 938 TGATCA BcnI 1998 CCGGG CC^[GC]GG 2254 CCGGG BfrI 217 CTTAAG C^TTAAG 2890 CTTAAG BsaWI 562 TCCGGA [AT]^CCGG[AT] 1472 TCCGGT BsmBI 610 CGTCTC CGTCTC(1/5) 1379 CGTCTC BsmFI 2165 GTCCC GGGAC(10/14) 2716 GTCCC BstUI 103 CGCG CG^CG 442 CGCG Bsu36I 849 CCTGAGG CC^T[ACGT]AGG 2067 CCTCAGG DraI 312 TTTAAA TTT^AAA 3055 TTTAAA DraIII 439 CACCGCGTG CAC[ACGT][ACGT][ACGT]^GTG 840 CACTGAGTG Eco81I 849 CCTGAGG CC^T[ACGT]AGG 2067 CCTCAGG EcoNI 468 CCTCCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 789 CCTGATAAAGG EcoRI 754 GAATTC G^AATTC 1972 GAATTC EcoRV 2026 GATATC GAT^ATC 2033 GATATC EcoT14I 357 CCTTGG C^C[AT][AT]GG 1403 CCAAGG FbaI 651 TGATCA T^GATCA 938 TGATCA MvnI 103 CGCG CG^CG 442 CGCG NciI 1998 CCGGG CC^[GC]GG 2254 CCGGG SapI 478 GCTCTTC GCTCTTC(1/4) 2599 GCTCTTC StuI 1576 AGGCCT AGG^CCT 2355 AGGCCT StyI 357 CCTTGG C^C[AT][AT]GG 1403 CCAAGG ######################################### # 3 OR MORE CUT ENZYMES ( 76 enzymes) # ######################################### AciI AcsI AfaI AluI ApoI Asp700I AvaII BanII BbsI BbvI BfaI BmyI BpmI BpuAI BsaJI BseRI BsiYI BslI BsmAI Bsp1286I BspHI BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII DraII EarI EcoO109I EcoRII Fnu4HI FokI HaeIII HapII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MseI MslI MspI MvaI MwoI NdeII NlaIII NlaIV PleI PstI RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XmnI