(Linear) (Six-base) MAPSORT of: KIBB0482.seq Check: 892 from: 1 to: 3072 KIBB0482.orf With 182 enzymes: SgfI * July 15, 2010 18:29 .. AatII G_ACGT'C Cuts at: 0 1381 3072 Size: 1381 1691 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 466 730 974 2372 2389 3072 Size: 466 264 244 1398 17 683 Fragments arranged by size: 1398 683 466 264 244 17 AflII C'TTAA_G Cuts at: 0 217 2890 3072 Size: 217 2673 182 Fragments arranged by size: 2673 217 182 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 164 560 887 3072 Size: 164 396 327 2185 Fragments arranged by size: 2185 396 327 164 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1186 1218 3072 Size: 1186 32 1854 Fragments arranged by size: 1854 1186 32 AlwNI CAG_nnn'CTG Cuts at: 0 409 2129 3072 Size: 409 1720 943 Fragments arranged by size: 1720 943 409 ApaI G_GGCC'C Cuts at: 0 624 3072 Size: 624 2448 ApoI r'AATT_y Cuts at: 0 3 592 754 971 1318 1631 1720 1972 Size: 3 589 162 217 347 313 89 252 Cuts at: 1972 2471 2774 2826 2926 3072 Size: 499 303 52 100 146 Fragments arranged by size: 589 499 347 313 303 252 217 162 146 100 89 52 3 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 1467 1500 3072 Size: 1467 33 1572 Fragments arranged by size: 1572 1467 33 BanI G'GyrC_C Cuts at: 0 871 3072 Size: 871 2201 BanII G_rGCy'C Cuts at: 0 30 490 562 624 1277 3072 Size: 30 460 72 62 653 1795 Fragments arranged by size: 1795 653 460 72 62 30 BbsI GAAGACnn'nnnn_ Cuts at: 0 591 911 1012 1365 1892 3072 Size: 591 320 101 353 527 1180 Fragments arranged by size: 1180 591 527 353 320 101 BbvCI CC'TCA_GC Cuts at: 0 884 2122 3072 Size: 884 1238 950 Fragments arranged by size: 1238 950 884 BclI T'GATC_A Cuts at: 0 651 938 3072 Size: 651 287 2134 Fragments arranged by size: 2134 651 287 BglI GCCn_nnn'nGGC Cuts at: 0 1507 3072 Size: 1507 1565 Bme1580I G_kGCm'C Cuts at: 0 624 876 1824 3072 Size: 624 252 948 1248 Fragments arranged by size: 1248 948 624 252 BmgBI CAC'GTC Cuts at: 0 2673 3072 Size: 2673 399 BmrI ACTGGGnnnn_n' Cuts at: 0 1280 3072 Size: 1280 1792 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1080 3072 Size: 1080 1992 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1213 1605 2005 2769 3072 Size: 1213 392 400 764 303 Fragments arranged by size: 1213 764 400 392 303 Bpu10I CC'TnA_GC Cuts at: 0 305 884 1711 2122 2686 3072 Size: 305 579 827 411 564 386 Fragments arranged by size: 827 579 564 411 386 305 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1011 2241 3072 Size: 1011 1230 831 Fragments arranged by size: 1230 1011 831 BsaI GGTCTCn'nnnn_ Cuts at: 0 1175 3072 Size: 1175 1897 BsaBI GATnn'nnATC Cuts at: 0 3029 3072 Size: 3029 43 BsaHI Gr'CG_yC Cuts at: 0 1378 3072 Size: 1378 1694 BsaWI w'CCGG_w Cuts at: 0 562 1472 3072 Size: 562 910 1600 Fragments arranged by size: 1600 910 562 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1186 1216 3072 Size: 1186 30 1856 Fragments arranged by size: 1856 1186 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1267 1408 2725 3072 Size: 1267 141 1317 347 Fragments arranged by size: 1317 1267 347 141 BseYI C'CCAG_C Cuts at: 0 1933 3072 Size: 1933 1139 BsmI GAATG_Cn' Cuts at: 0 2552 3072 Size: 2552 520 BsmBI CGTCTCn'nnnn_ Cuts at: 0 616 1385 3072 Size: 616 769 1687 Fragments arranged by size: 1687 769 616 Bsp1286I G_dGCh'C Cuts at: 0 30 490 562 624 876 1277 1824 3072 Size: 30 460 72 62 252 401 547 1248 Fragments arranged by size: 1248 547 460 401 252 72 62 30 BspEI T'CCGG_A Cuts at: 0 562 3072 Size: 562 2510 BspHI T'CATG_A Cuts at: 0 1001 1383 2229 2604 3072 Size: 1001 382 846 375 468 Fragments arranged by size: 1001 846 468 382 375 BstBI TT'CG_AA Cuts at: 0 1223 3072 Size: 1223 1849 BstEII G'GTnAC_C Cuts at: 0 1398 3072 Size: 1398 1674 Bsu36I CC'TnA_GG Cuts at: 0 850 2068 3072 Size: 850 1218 1004 Fragments arranged by size: 1218 1004 850 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1551 3072 Size: 1551 1521 BtsI GCAGTG_nn' Cuts at: 0 1538 2513 2856 3072 Size: 1538 975 343 216 Fragments arranged by size: 1538 975 343 216 DraI TTT'AAA Cuts at: 0 314 3057 3072 Size: 314 2743 15 Fragments arranged by size: 2743 314 15 DraIII CAC_nnn'GTG Cuts at: 0 444 845 3072 Size: 444 401 2227 Fragments arranged by size: 2227 444 401 DrdI GACnn_nn'nnGTC Cuts at: 0 2400 3072 Size: 2400 672 EaeI y'GGCC_r Cuts at: 0 698 3072 Size: 698 2374 EarI CTCTTCn'nnn_ Cuts at: 0 485 1904 2606 3072 Size: 485 1419 702 466 Fragments arranged by size: 1419 702 485 466 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 466 730 974 1213 1605 2005 2372 2389 Size: 466 264 244 239 392 400 367 17 Cuts at: 2389 2769 3072 Size: 380 303 Fragments arranged by size: 466 400 392 380 367 303 264 244 239 17 EcoNI CCTnn'n_nnAGG Cuts at: 0 472 793 3072 Size: 472 321 2279 Fragments arranged by size: 2279 472 321 EcoO109I rG'GnC_Cy Cuts at: 0 620 1914 2715 3072 Size: 620 1294 801 357 Fragments arranged by size: 1294 801 620 357 EcoRI G'AATT_C Cuts at: 0 754 1972 3072 Size: 754 1218 1100 Fragments arranged by size: 1218 1100 754 EcoRV GAT'ATC Cuts at: 0 2028 2035 3072 Size: 2028 7 1037 Fragments arranged by size: 2028 1037 7 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 3019 3072 Size: 3019 53 HaeII r_GCGC'y Cuts at: 0 914 3072 Size: 914 2158 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1346 1378 1435 1467 1550 1582 1681 1713 Size: 1346 32 57 32 83 32 99 32 Cuts at: 1713 3072 Size: 1359 Fragments arranged by size: 1359 1346 99 83 57 32 32 32 32 HincII GTy'rAC Cuts at: 0 2981 3072 Size: 2981 91 HindIII A'AGCT_T Cuts at: 0 1727 1746 2731 3072 Size: 1727 19 985 341 Fragments arranged by size: 1727 985 341 19 HpaI GTT'AAC Cuts at: 0 2981 3072 Size: 2981 91 MfeI C'AATT_G Cuts at: 0 1809 3072 Size: 1809 1263 MscI TGG'CCA Cuts at: 0 700 3072 Size: 700 2372 MslI CAynn'nnrTG Cuts at: 0 166 700 1136 2320 3072 Size: 166 534 436 1184 752 Fragments arranged by size: 1184 752 534 436 166 MspA1I CmG'CkG Cuts at: 0 453 3072 Size: 453 2619 NruI TCG'CGA Cuts at: 0 104 3072 Size: 104 2968 PasI CC'CwG_GG Cuts at: 0 624 2660 3072 Size: 624 2036 412 Fragments arranged by size: 2036 624 412 PflMI CCAn_nnn'nTGG Cuts at: 0 554 3072 Size: 554 2518 PfoI T'CCnGG_A Cuts at: 0 659 1337 1997 3072 Size: 659 678 660 1075 Fragments arranged by size: 1075 678 660 659 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1186 1218 3072 Size: 1186 32 1854 Fragments arranged by size: 1854 1186 32 PpuMI rG'GwC_Cy Cuts at: 0 2715 3072 Size: 2715 357 PsiI TTA'TAA Cuts at: 0 58 3072 Size: 58 3014 PspOMI G'GGCC_C Cuts at: 0 620 3072 Size: 620 2452 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 77 109 3072 Size: 77 32 2963 Fragments arranged by size: 2963 77 32 PstI C_TGCA'G Cuts at: 0 476 539 1232 1884 3072 Size: 476 63 693 652 1188 Fragments arranged by size: 1188 693 652 476 63 PvuII CAG'CTG Cuts at: 0 453 3072 Size: 453 2619 SapI GCTCTTCn'nnn_ Cuts at: 0 485 2606 3072 Size: 485 2121 466 Fragments arranged by size: 2121 485 466 SbfI CC_TGCA'GG Cuts at: 0 476 3072 Size: 476 2596 ScaI AGT'ACT Cuts at: 0 2919 3072 Size: 2919 153 SfcI C'TryA_G Cuts at: 0 472 535 1228 1880 2236 3072 Size: 472 63 693 652 356 836 Fragments arranged by size: 836 693 652 472 356 63 SmlI C'TyrA_G Cuts at: 0 217 990 2220 2890 3072 Size: 217 773 1230 670 182 Fragments arranged by size: 1230 773 670 217 182 SpeI A'CTAG_T Cuts at: 0 3060 3072 Size: 3060 12 SspI AAT'ATT Cuts at: 0 2048 3072 Size: 2048 1024 StuI AGG'CCT Cuts at: 0 1578 2357 3072 Size: 1578 779 715 Fragments arranged by size: 1578 779 715 StyI C'CwwG_G Cuts at: 0 357 1403 3072 Size: 357 1046 1669 Fragments arranged by size: 1669 1046 357 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1036 2863 2999 3072 Size: 1036 1827 136 73 Fragments arranged by size: 1827 1036 136 73 TatI w'GTAC_w Cuts at: 0 1343 1693 2917 3072 Size: 1343 350 1224 155 Fragments arranged by size: 1343 1224 350 155 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2828 2860 3072 Size: 2828 32 212 Fragments arranged by size: 2828 212 32 Tth111I GACn'n_nGTC Cuts at: 0 260 3072 Size: 260 2812 XmnI GAAnn'nnTTC Cuts at: 0 2047 2471 2600 2812 3072 Size: 2047 424 129 212 260 Fragments arranged by size: 2047 424 260 212 129 ZraI GAC'GTC Cuts at: 0 1379 3072 Size: 1379 1693 Enzymes that do cut: AatII AcuI AflII AlfI AloI AlwNI ApaI ApoI BaeI BanI BanII BbsI BbvCI BclI BglI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsmI BsmBI Bsp1286I BspEI BspHI BstBI BstEII Bsu36I BtgZI BtsI DraI DraIII DrdI EaeI EarI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI HaeII Hin4I HincII HindIII HpaI MfeI MscI MslI MspA1I NruI PasI PflMI PfoI PpiI PpuMI PsiI PspOMI PsrI PstI PvuII SapI SbfI ScaI SfcI SmlI SpeI SspI StuI StyI TaqII TatI TstI Tth111I XmnI ZraI Enzymes that do not cut: AarI AccI Acc65I AclI AfeI AflIII AgeI AhdI AleI ApaLI AscI AseI AsiSI AvaI AvrII BamHI BbeI BcgI BciVI BfrBI BglII BlpI BmtI BsaAI BsgI BsiEI BsiHKAI BsiWI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstXI BstYI BstZ17I BtgI ClaI CspCI EagI EciI EcoICRI FseI FspI FspAI KasI KpnI MluI MmeI NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PacI PciI PmeI PmlI PshAI PspXI PvuI RsrII SacI SacII SalI SanDI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SphI SrfI SwaI XbaI XcmI XhoI XmaI