################################## # NO CUT ENZYMES ( 127 enzymes) # ################################## AatII Acc65I AccII AccIII AciI AclI AcyI AflII AgeI AhdI Aor51HI ApaI AscI AseI AsnI Asp718I AspEI AviII AvrII BalI BbeI BbrPI BbvCI BcgI BciVI BcnI BfrI BglI BlnI BsaAI BsaBI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BspDI BspEI BsrBI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstZ17I CfoI Cfr10I ClaI CpoI DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoNI FauI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HpaI HpaII KasI KpnI KspI MamI MfeI MluI MluNI MroI MscI MspI MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SwaI Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AccI 2310 GTCTAC GT^[AC][GT]AC Alw44I 1661 GTGCAC G^TGCAC AocI 2725 CCTCAGG CC^T[ACGT]AGG ApaLI 1661 GTGCAC G^TGCAC AvaI 670 CTCGGG C^[CT]CG[GA]G BamHI 2451 GGATCC G^GATCC BanII 2499 GAGCTC G[GA]GC[CT]^C BclI 2006 TGATCA T^GATCA BglII 1419 AGATCT A^GATCT BlpI 156 GCTGAGC GC^T[ACGT]AGC Bpu1102I 156 GCTGAGC GC^T[ACGT]AGC BsmFI 952 GTCCC GGGAC(10/14) BsoBI 670 CTCGGG C^[CT]CG[GA]G Bsp1407I 528 TGTACA T^GTACA BspHI 1119 TCATGA T^CATGA BspLU11I 465 ACATGT A^CATGT BsrDI 1622 GCAATG GCAATG(2/0) BsrGI 528 TGTACA T^GTACA BstEII 793 GGTGACC G^GT[ACGT]ACC BstPI 793 GGTGACC G^GT[ACGT]ACC BstXI 42 CCAGAAGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 2725 CCTCAGG CC^T[ACGT]AGG CelII 156 GCTGAGC GC^T[ACGT]AGC DraI 1429 TTTAAA TTT^AAA DraII 1514 GGGTCCT [GA]G^G[ACGT]CC[CT] DsaI 955 CCATGG C^C[GA][CT]GG Eco81I 2725 CCTCAGG CC^T[ACGT]AGG EcoO109I 1514 GGGTCCT [GA]G^G[ACGT]CC[CT] EcoO65I 793 GGTGACC G^GT[ACGT]ACC EcoRI 1410 GAATTC G^AATTC EcoRV 3 GATATC GAT^ATC EcoT22I 1228 ATGCAT ATGCA^T FbaI 2006 TGATCA T^GATCA HindIII 1316 AAGCTT A^AGCTT MslI 1248 CACCAAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NcoI 955 CCATGG C^CATGG NsiI 1228 ATGCAT ATGCA^T PpuMI 1514 GGGTCCT [GA]G^G[AT]CC[CT] PstI 847 CTGCAG CTGCA^G RcaI 1119 TCATGA T^CATGA SacI 2499 GAGCTC GAGCT^C SapI 1438 GAAGAGC GCTCTTC(1/4) SspBI 528 TGTACA T^GTACA SspI 1089 AATATT AAT^ATT TaqI 2033 TCGA T^CGA TthHB8I 2033 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### AflIII 236 ACGTGT A^C[GA][CT]GT 465 ACATGT AlwNI 841 CAGCAACTG CAG[ACGT][ACGT][ACGT]^CTG 1937 CAGTGGCTG AspI 203 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC 2116 GACAGTGTC BbsI 45 GAAGAC GAAGAC(2/6) 1156 GAAGAC BpuAI 45 GAAGAC GAAGAC(2/6) 1156 GAAGAC BseRI 2215 GAGGAG GAGGAG(10/8) 2665 GAGGAG BsgI 1372 CTGCAC GTGCAG(16/14) 1877 CTGCAC BsmI 888 GAATGC GAATGC(1/-1) 1797 GAATGC BspMI 212 ACCTGC ACCTGC(4/8) 1370 ACCTGC MaeII 236 ACGT A^CGT 2438 ACGT MwoI 843 GCAACTGCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 1622 GCAATGAAAGC PflFI 203 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC 2116 GACAGTGTC StuI 2462 AGGCCT AGG^CCT 2552 AGGCCT Tth111I 203 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC 2116 GACAGTGTC XcmI 1760 CCAGTGATTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1818 CCAGAGAGACTGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 70 enzymes) # ######################################### AcsI AfaI AluI AlwI ApoI Asp700I AspHI AvaII BanI BbvI BfaI BmyI BpmI BsaI BsaJI BsiHKAI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII EarI EcoRII EcoT14I Fnu4HI FokI HaeIII HinfI HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MspA1I MvaI NdeII NlaIII NlaIV NspI PleI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XhoII XmnI