(Circular) (Six-base) MAPSORT of: pFN21AE3528.seq Check: 3971 from: 1 to: 7441 pFN21AE3528 7441 bp With 182 enzymes: SgfI * July 6, 2012 17:33 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2226 2226 Size: 7441 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5251 278 Size: 53 83 186 936 3715 2468 Fragments arranged by size: 3715 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4316 4784 1937 Size: 2379 468 4594 Fragments arranged by size: 4594 2379 468 Acc65I G'GTAC_C Cuts at: 4762 4762 Size: 7441 AclI AA'CG_TT Cuts at: 1563 5568 5941 1563 Size: 4005 373 3063 Fragments arranged by size: 4005 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2402 2576 4061 4229 5504 6888 7156 1632 Size: 770 174 1485 168 1275 1384 268 1917 Fragments arranged by size: 1917 1485 1384 1275 770 268 174 168 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7244 AflIII A'CryG_T Cuts at: 1203 2241 2470 6340 7317 1203 Size: 1038 229 3870 977 1327 Fragments arranged by size: 3870 1327 1038 977 229 AgeI A'CCGG_T Cuts at: 6272 6272 Size: 7441 AhdI GACnn_n'nnGTC Cuts at: 6170 6170 Size: 7441 AleI CACnn'nnGTG Cuts at: 1166 3257 1166 Size: 2091 5350 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2393 3135 1184 Size: 1209 742 5490 Fragments arranged by size: 5490 1209 742 AlwNI CAG_nnn'CTG Cuts at: 2851 3947 5184 6756 2851 Size: 1096 1237 1572 3536 Fragments arranged by size: 3536 1572 1237 1096 ApaLI G'TGCA_C Cuts at: 3666 5498 6654 3666 Size: 1832 1156 4453 Fragments arranged by size: 4453 1832 1156 ApoI r'AATT_y Cuts at: 1493 2037 2141 2151 3217 3415 4238 4513 4750 Size: 544 104 10 1066 198 823 275 237 Cuts at: 4750 4976 5128 7237 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 1066 823 544 275 237 226 198 152 104 10 AseI AT'TA_AT Cuts at: 160 1032 5995 160 Size: 872 4963 1606 Fragments arranged by size: 4963 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7441 AvaI C'yCGr_G Cuts at: 1870 1943 2675 4766 1870 Size: 73 732 2091 4545 Fragments arranged by size: 4545 2091 732 73 BamHI G'GATC_C Cuts at: 4456 4771 5138 4456 Size: 315 367 6759 Fragments arranged by size: 6759 367 315 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2824 4057 4252 4762 6217 Size: 325 217 627 1037 1233 195 510 1455 Cuts at: 6217 618 Size: 1842 Fragments arranged by size: 1842 1455 1233 1037 627 510 325 217 195 BanII G_rGCy'C Cuts at: 1391 4508 4760 1391 Size: 3117 252 4072 Fragments arranged by size: 4072 3117 252 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2057 3168 928 Size: 971 158 1111 5201 Fragments arranged by size: 5201 1111 971 158 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5632 5666 1903 Size: 34 3695 34 3678 Fragments arranged by size: 3695 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5333 6543 5333 Size: 1210 6231 BclI T'GATC_A Cuts at: 1548 4011 1548 Size: 2463 4978 BfrBI ATG'CAT Cuts at: 3235 3235 Size: 7441 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6052 136 Size: 107 122 71 631 605 4380 1525 Fragments arranged by size: 4380 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3424 7436 3424 Size: 4012 3429 BlpI GC'TnA_GC Cuts at: 2162 4858 2162 Size: 2696 4745 Bme1580I G_kGCm'C Cuts at: 1790 3670 4363 5502 6658 1790 Size: 1880 693 1139 1156 2573 Fragments arranged by size: 2573 1880 1156 1139 693 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7441 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2064 3204 4373 Size: 416 53 83 399 24 647 1140 1169 Cuts at: 4373 5172 6130 442 Size: 799 958 1753 Fragments arranged by size: 1753 1169 1140 958 799 647 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7441 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3499 4190 4245 4448 6101 1239 Size: 2260 691 55 203 1653 2579 Fragments arranged by size: 2579 2260 1653 691 203 55 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2934 3326 3676 4170 5566 6431 6729 2934 Size: 392 350 494 1396 865 298 3646 Fragments arranged by size: 3646 1396 865 494 392 350 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3858 4295 4595 6104 882 Size: 617 2359 437 300 1509 2219 Fragments arranged by size: 2359 2219 1509 617 437 300 BsaAI yAC'GTr Cuts at: 493 1204 5232 493 Size: 711 4028 2702 Fragments arranged by size: 4028 2702 711 BsaBI GATnn'nnATC Cuts at: 7435 7435 Size: 7441 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5248 5630 6265 275 Size: 53 83 186 936 3715 382 635 1451 Fragments arranged by size: 3715 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5874 6272 6546 6693 7104 5874 Size: 398 274 147 411 6211 Fragments arranged by size: 6211 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 7216 Fragments arranged by size: 7216 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 4235 4685 1186 Size: 3049 450 3942 Fragments arranged by size: 3942 3049 450 BseYI C'CCAG_C Cuts at: 3003 6644 7140 3003 Size: 3641 496 3304 Fragments arranged by size: 3641 3304 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3362 3867 1163 Size: 573 1626 505 4737 Fragments arranged by size: 4737 1626 573 505 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4891 5652 6680 1540 Size: 275 186 2890 761 1028 2301 Fragments arranged by size: 2890 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2790 3670 4508 5502 5587 6658 2790 Size: 880 838 994 85 1071 3573 Fragments arranged by size: 3573 1071 994 880 838 85 BsmI GAATG_Cn' Cuts at: 2899 3808 4952 5045 2899 Size: 909 1144 93 5295 Fragments arranged by size: 5295 1144 909 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2790 3670 4363 4508 4760 5502 5587 Size: 399 1000 880 693 145 252 742 85 Cuts at: 5587 6658 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 1000 880 742 693 399 252 145 85 BspEI T'CCGG_A Cuts at: 7104 7104 Size: 7441 BspHI T'CATG_A Cuts at: 3124 5330 7060 3124 Size: 2206 1730 3505 Fragments arranged by size: 3505 2206 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2226 3384 4796 844 Size: 1382 1158 1412 3489 Fragments arranged by size: 3489 1412 1382 1158 BsrBI CCG'CTC Cuts at: 1394 1823 5328 1394 Size: 429 3505 3507 Fragments arranged by size: 3507 3505 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3634 5936 6110 65 Size: 1183 2386 2302 174 1396 Fragments arranged by size: 2386 2302 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6085 6272 1700 Size: 110 4275 187 2869 Fragments arranged by size: 4275 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2533 96 Size: 2437 5004 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7441 BssSI C'ACGA_G Cuts at: 5501 6513 5501 Size: 1012 6429 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7441 BstBI TT'CG_AA Cuts at: 6245 6245 Size: 7441 BstEII G'GTnAC_C Cuts at: 1184 2798 1184 Size: 1614 5827 BstXI CCAn_nnnn'nTGG Cuts at: 2054 2054 Size: 7441 BstYI r'GATC_y Cuts at: 1972 3424 4181 4456 4771 5138 5523 5540 6992 Size: 1452 757 275 315 367 385 17 1452 Cuts at: 6992 7078 7090 7436 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1452 757 385 367 346 315 275 86 17 12 Bsu36I CC'TnA_GG Cuts at: 4731 4731 Size: 7441 BtgI C'CryG_G Cuts at: 513 1065 2960 513 Size: 552 1895 4994 Fragments arranged by size: 4994 1895 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4548 487 Size: 683 56 3322 3380 Fragments arranged by size: 3380 3322 683 56 BtsI GCAGTG_nn' Cuts at: 4217 4957 5751 5771 4217 Size: 740 794 20 5887 Fragments arranged by size: 5887 794 740 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7441 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3384 3419 529 Size: 35 2820 35 4551 Fragments arranged by size: 4551 2820 35 35 DraI TTT'AAA Cuts at: 3436 4745 3436 Size: 1309 6132 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6448 809 Size: 507 5132 1802 Fragments arranged by size: 5132 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4888 5777 7406 Size: 54 1425 50 275 18 3058 889 1629 Cuts at: 7406 8 Size: 43 Fragments arranged by size: 3058 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4888 1537 Size: 275 3076 4090 Fragments arranged by size: 4090 3076 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3438 3473 3654 3984 4293 5371 1353 Size: 2085 35 181 330 309 1078 3423 Fragments arranged by size: 3423 2085 1078 330 309 181 35 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6028 6402 6548 387 Size: 1064 138 4439 374 146 1280 Fragments arranged by size: 4439 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4506 4506 Size: 7441 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2402 2576 3499 4061 4190 4229 4245 Size: 393 770 174 923 562 129 39 16 Cuts at: 4245 4448 5504 6101 6888 7156 1239 Size: 203 1056 597 787 268 1524 Fragments arranged by size: 1524 1056 923 787 770 597 562 393 268 203 174 129 39 16 EcoO109I rG'GnC_Cy Cuts at: 3520 3520 Size: 7441 EcoRI G'AATT_C Cuts at: 3415 4750 5128 3415 Size: 1335 378 5728 Fragments arranged by size: 5728 1335 378 EcoRV GAT'ATC Cuts at: 2010 2010 Size: 7441 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3464 3633 3464 Size: 169 7272 FspI TGC'GCA Cuts at: 5947 5947 Size: 7441 HaeII r_GCGC'y Cuts at: 1678 6588 1678 Size: 4910 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2249 2281 4294 4326 4441 Size: 32 135 32 677 32 2013 32 115 Cuts at: 4441 4473 6082 6114 6156 6188 1373 Size: 32 1609 32 42 32 2626 Fragments arranged by size: 2626 2013 1609 677 135 115 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4785 5031 669 Size: 988 281 2847 246 3079 Fragments arranged by size: 3079 2847 988 281 246 HindIII A'AGCT_T Cuts at: 748 3321 748 Size: 2573 4868 HpaI GTT'AAC Cuts at: 5031 5031 Size: 7441 KpnI G_GTAC'C Cuts at: 4766 4766 Size: 7441 MfeI C'AATT_G Cuts at: 5040 5040 Size: 7441 MluI A'CGCG_T Cuts at: 7317 7317 Size: 7441 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6555 6739 1305 Size: 5250 184 2007 Fragments arranged by size: 5250 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5619 Fragments arranged by size: 5619 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3257 5399 5758 5917 518 Size: 648 2091 2142 359 159 2042 Fragments arranged by size: 2142 2091 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2161 3007 3154 4857 5534 6682 6927 1606 Size: 555 846 147 1703 677 1148 245 2120 Fragments arranged by size: 2120 1703 1148 846 677 555 245 147 NcoI C'CATG_G Cuts at: 513 1065 2960 513 Size: 552 1895 4994 Fragments arranged by size: 4994 1895 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7441 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7441 NotI GC'GGCC_GC Cuts at: 4888 4888 Size: 7441 NsiI A_TGCA'T Cuts at: 3237 3237 Size: 7441 NspI r_CATG'y Cuts at: 1132 1229 2474 3112 3239 4654 4799 6344 1132 Size: 97 1245 638 127 1415 145 1545 2229 Fragments arranged by size: 2229 1545 1415 1245 638 145 127 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7441 PciI A'CATG_T Cuts at: 2470 6340 2470 Size: 3870 3571 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7441 PfoI T'CCnGG_A Cuts at: 2298 3690 2298 Size: 1392 6049 PmeI GTTT'AAAC Cuts at: 4745 4745 Size: 7441 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5510 5542 7023 7055 5510 Size: 32 1481 32 5896 Fragments arranged by size: 5896 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3520 3520 Size: 7441 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7441 PsiI TTA'TAA Cuts at: 2147 5011 2147 Size: 2864 4577 PstI C_TGCA'G Cuts at: 830 2856 4793 830 Size: 2026 1937 3478 Fragments arranged by size: 3478 2026 1937 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7441 PvuII CAG'CTG Cuts at: 2161 3007 3154 2161 Size: 846 147 6448 Fragments arranged by size: 6448 846 147 SacI G_AGCT'C Cuts at: 4508 4508 Size: 7441 SalI G'TCGA_C Cuts at: 1936 4783 1936 Size: 2847 4594 SapI GCTCTTCn'nnn_ Cuts at: 3438 3438 Size: 7441 SbfI CC_TGCA'GG Cuts at: 4793 4793 Size: 7441 ScaI AGT'ACT Cuts at: 5689 7401 5689 Size: 1712 5729 SfcI C'TryA_G Cuts at: 826 1045 2269 2852 3028 3544 4317 4471 4789 Size: 219 1224 583 176 516 773 154 318 Cuts at: 4789 5924 6605 6796 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1224 1135 773 681 583 516 318 219 191 176 154 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7441 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7441 SmaI CCC'GGG Cuts at: 1872 4768 1872 Size: 2896 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2949 3341 3655 4185 5545 6446 Size: 197 926 1006 392 314 530 1360 901 Cuts at: 6446 6708 820 Size: 262 1553 Fragments arranged by size: 1553 1360 1006 926 901 530 392 314 262 197 SnaBI TAC'GTA Cuts at: 493 5232 493 Size: 4739 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7441 SphI G_CATG'C Cuts at: 1132 4799 1132 Size: 3667 3774 SspI AAT'ATT Cuts at: 5 52 3096 5365 5 Size: 47 3044 2269 2081 Fragments arranged by size: 3044 2269 2081 47 StuI AGG'CCT Cuts at: 1517 4469 4559 1517 Size: 2952 90 4399 Fragments arranged by size: 4399 2952 90 StyI C'CwwG_G Cuts at: 513 1065 2960 4354 4425 4880 513 Size: 552 1895 1394 71 455 3074 Fragments arranged by size: 3074 1895 1394 552 455 71 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5468 5485 5638 5823 6268 325 Size: 1255 3888 17 153 185 445 1498 Fragments arranged by size: 3888 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2533 3032 5687 Size: 274 80 33 51 1443 556 499 2655 Cuts at: 5687 7399 96 Size: 1712 138 Fragments arranged by size: 2655 1712 1443 556 499 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3190 3222 7401 7433 3190 Size: 32 4179 32 3198 Fragments arranged by size: 4179 3198 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2211 4124 1316 Size: 895 1913 4633 Fragments arranged by size: 4633 1913 895 XbaI T'CTAG_A Cuts at: 4777 4777 Size: 7441 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3772 3830 3772 Size: 58 7383 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7441 XmaI C'CCGG_G Cuts at: 1870 4766 1870 Size: 2896 4545 XmnI GAAnn'nnTTC Cuts at: 2294 2970 3023 4242 5570 2294 Size: 676 53 1219 1328 4165 Fragments arranged by size: 4165 1328 1219 676 53 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5249 276 Size: 53 83 186 936 3715 2468 Fragments arranged by size: 3715 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI