(Linear) (Six-base) MAPSORT of: KIEE3528.seq Check: 9810 from: 1 to: 2736 KIEE3528.orf With 182 enzymes: SgfI * July 3, 2012 18:05 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 221 2736 Size: 221 2515 AccI GT'mk_AC Cuts at: 0 2311 2736 Size: 2311 425 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 397 571 2056 2224 2736 Size: 397 174 1485 168 512 Fragments arranged by size: 1485 512 397 174 168 AflIII A'CryG_T Cuts at: 0 236 465 2736 Size: 236 229 2271 Fragments arranged by size: 2271 236 229 AleI CACnn'nnGTG Cuts at: 0 1252 2736 Size: 1252 1484 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 388 1130 2736 Size: 388 742 1606 Fragments arranged by size: 1606 742 388 AlwNI CAG_nnn'CTG Cuts at: 0 846 1942 2736 Size: 846 1096 794 Fragments arranged by size: 1096 846 794 ApaLI G'TGCA_C Cuts at: 0 1661 2736 Size: 1661 1075 ApoI r'AATT_y Cuts at: 0 32 136 146 1212 1410 2233 2508 2736 Size: 32 104 10 1066 198 823 275 228 Fragments arranged by size: 1066 823 275 228 198 104 32 10 AvaI C'yCGr_G Cuts at: 0 670 2736 Size: 670 2066 BamHI G'GATC_C Cuts at: 0 2451 2736 Size: 2451 285 BanI G'GyrC_C Cuts at: 0 819 2052 2247 2736 Size: 819 1233 195 489 Fragments arranged by size: 1233 819 489 195 BanII G_rGCy'C Cuts at: 0 2503 2736 Size: 2503 233 BbsI GAAGACnn'nnnn_ Cuts at: 0 52 1163 2736 Size: 52 1111 1573 Fragments arranged by size: 1573 1111 52 BclI T'GATC_A Cuts at: 0 2006 2736 Size: 2006 730 BfrBI ATG'CAT Cuts at: 0 1230 2736 Size: 1230 1506 BglII A'GATC_T Cuts at: 0 1419 2736 Size: 1419 1317 BlpI GC'TnA_GC Cuts at: 0 157 2736 Size: 157 2579 Bme1580I G_kGCm'C Cuts at: 0 1665 2358 2736 Size: 1665 693 378 Fragments arranged by size: 1665 693 378 BmrI ACTGGGnnnn_n' Cuts at: 0 59 1199 2368 2736 Size: 59 1140 1169 368 Fragments arranged by size: 1169 1140 368 59 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1494 2185 2240 2443 2736 Size: 1494 691 55 203 293 Fragments arranged by size: 1494 691 293 203 55 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 929 1321 1671 2165 2736 Size: 929 392 350 494 571 Fragments arranged by size: 929 571 494 392 350 BsaI GGTCTCn'nnnn_ Cuts at: 0 1853 2290 2590 2736 Size: 1853 437 300 146 Fragments arranged by size: 1853 437 300 146 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 2230 2680 2736 Size: 2230 450 56 Fragments arranged by size: 2230 450 56 BseYI C'CCAG_C Cuts at: 0 998 2736 Size: 998 1738 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1357 1862 2736 Size: 1357 505 874 Fragments arranged by size: 1357 874 505 BsiHKAI G_wGCw'C Cuts at: 0 785 1665 2503 2736 Size: 785 880 838 233 Fragments arranged by size: 880 838 785 233 BsmI GAATG_Cn' Cuts at: 0 894 1803 2736 Size: 894 909 933 Fragments arranged by size: 933 909 894 Bsp1286I G_dGCh'C Cuts at: 0 785 1665 2358 2503 2736 Size: 785 880 693 145 233 Fragments arranged by size: 880 785 693 233 145 BspHI T'CATG_A Cuts at: 0 1119 2736 Size: 1119 1617 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 221 1379 2736 Size: 221 1158 1357 Fragments arranged by size: 1357 1158 221 BsrDI GCAATG_nn' Cuts at: 0 1629 2736 Size: 1629 1107 BsrGI T'GTAC_A Cuts at: 0 528 2736 Size: 528 2208 BstEII G'GTnAC_C Cuts at: 0 793 2736 Size: 793 1943 BstXI CCAn_nnnn'nTGG Cuts at: 0 49 2736 Size: 49 2687 BstYI r'GATC_y Cuts at: 0 1419 2176 2451 2736 Size: 1419 757 275 285 Fragments arranged by size: 1419 757 285 275 Bsu36I CC'TnA_GG Cuts at: 0 2726 2736 Size: 2726 10 BtgI C'CryG_G Cuts at: 0 955 2736 Size: 955 1781 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 2543 2736 Size: 2543 193 BtsI GCAGTG_nn' Cuts at: 0 2212 2736 Size: 2212 524 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1379 1414 2736 Size: 1379 35 1322 Fragments arranged by size: 1379 1322 35 DraI TTT'AAA Cuts at: 0 1431 2736 Size: 1431 1305 EarI CTCTTCn'nnn_ Cuts at: 0 1433 1468 1649 1979 2288 2736 Size: 1433 35 181 330 309 448 Fragments arranged by size: 1433 448 330 309 181 35 EcoICRI GAG'CTC Cuts at: 0 2501 2736 Size: 2501 235 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 397 571 1494 2056 2185 2224 2240 2443 Size: 397 174 923 562 129 39 16 203 Cuts at: 2443 2736 Size: 293 Fragments arranged by size: 923 562 397 293 203 174 129 39 16 EcoO109I rG'GnC_Cy Cuts at: 0 1515 2736 Size: 1515 1221 EcoRI G'AATT_C Cuts at: 0 1410 2736 Size: 1410 1326 EcoRV GAT'ATC Cuts at: 0 5 2736 Size: 5 2731 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1459 1628 2736 Size: 1459 169 1108 Fragments arranged by size: 1459 1108 169 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 244 276 2289 2321 2436 2468 2736 Size: 244 32 2013 32 115 32 268 Fragments arranged by size: 2013 268 244 115 32 32 32 HindIII A'AGCT_T Cuts at: 0 1316 2736 Size: 1316 1420 MslI CAynn'nnrTG Cuts at: 0 1252 2736 Size: 1252 1484 MspA1I CmG'CkG Cuts at: 0 156 1002 1149 2736 Size: 156 846 147 1587 Fragments arranged by size: 1587 846 156 147 NcoI C'CATG_G Cuts at: 0 955 2736 Size: 955 1781 NsiI A_TGCA'T Cuts at: 0 1232 2736 Size: 1232 1504 NspI r_CATG'y Cuts at: 0 469 1107 1234 2649 2736 Size: 469 638 127 1415 87 Fragments arranged by size: 1415 638 469 127 87 PciI A'CATG_T Cuts at: 0 465 2736 Size: 465 2271 PfoI T'CCnGG_A Cuts at: 0 293 1685 2736 Size: 293 1392 1051 Fragments arranged by size: 1392 1051 293 PpuMI rG'GwC_Cy Cuts at: 0 1515 2736 Size: 1515 1221 PsiI TTA'TAA Cuts at: 0 142 2736 Size: 142 2594 PstI C_TGCA'G Cuts at: 0 851 2736 Size: 851 1885 PvuII CAG'CTG Cuts at: 0 156 1002 1149 2736 Size: 156 846 147 1587 Fragments arranged by size: 1587 846 156 147 SacI G_AGCT'C Cuts at: 0 2503 2736 Size: 2503 233 SapI GCTCTTCn'nnn_ Cuts at: 0 1433 2736 Size: 1433 1303 SfcI C'TryA_G Cuts at: 0 264 847 1023 1539 2312 2466 2736 Size: 264 583 176 516 773 154 270 Fragments arranged by size: 773 583 516 270 264 176 154 SmlI C'TyrA_G Cuts at: 0 944 1336 1650 2180 2736 Size: 944 392 314 530 556 Fragments arranged by size: 944 556 530 392 314 SspI AAT'ATT Cuts at: 0 1091 2736 Size: 1091 1645 StuI AGG'CCT Cuts at: 0 2464 2554 2736 Size: 2464 90 182 Fragments arranged by size: 2464 182 90 StyI C'CwwG_G Cuts at: 0 955 2349 2420 2736 Size: 955 1394 71 316 Fragments arranged by size: 1394 955 316 71 TatI w'GTAC_w Cuts at: 0 528 1027 2736 Size: 528 499 1709 Fragments arranged by size: 1709 528 499 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1185 1217 2736 Size: 1185 32 1519 Fragments arranged by size: 1519 1185 32 Tth111I GACn'n_nGTC Cuts at: 0 206 2119 2736 Size: 206 1913 617 Fragments arranged by size: 1913 617 206 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1767 1825 2736 Size: 1767 58 911 Fragments arranged by size: 1767 911 58 XmnI GAAnn'nnTTC Cuts at: 0 289 965 1018 2237 2736 Size: 289 676 53 1219 499 Fragments arranged by size: 1219 676 499 289 53 Enzymes that do cut: AarI AccI AcuI AflIII AleI AlfI AlwNI ApaLI ApoI AvaI BamHI BanI BanII BbsI BclI BfrBI BglII BlpI Bme1580I BmrI BpmI BpuEI BsaI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrDI BsrGI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI EarI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI Hin4I HindIII MslI MspA1I NcoI NsiI NspI PciI PfoI PpuMI PsiI PstI PvuII SacI SapI SfcI SmlI SspI StuI StyI TatI TstI Tth111I XcmI XmnI Enzymes that do not cut: AatII Acc65I AclI AfeI AflII AgeI AhdI AloI ApaI AscI AseI AsiSI AvrII BaeI BbeI BbvCI BcgI BciVI BglI BmgBI BmtI BplI Bpu10I BsaAI BsaBI BsaHI BsaWI BsaXI BsiEI BsiWI BsmBI BspEI BsrBI BsrFI BssHII BssSI BstAPI BstBI BstZ17I ClaI DraIII DrdI EaeI EagI EciI EcoNI FseI FspI FspAI HaeII HincII HpaI KasI KpnI MfeI MluI MmeI MscI NaeI NarI NdeI NgoMIV NheI NotI NruI PacI PasI PflMI PmeI PmlI PpiI PshAI PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SwaI TaqII XbaI XhoI XmaI ZraI