################################## # NO CUT ENZYMES ( 147 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI Alw44I AocI Aor51HI ApaI ApaLI ApoI AseI AsnI Asp700I Asp718I AspHI AspI AvaII AviII AvrII BalI BbrPI BbvCI BcgI BciVI BclI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrGI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII ClaI CpoI DdeI DraI DraIII DrdI EaeI EagI EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KpnI Ksp632I KspI MaeII MaeIII MamI MfeI MluI MluNI MroI MscI MseI MslI MunI NcoI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI ScaI SexAI SfaNI SfiI SfuI SmlI SnaBI SpeI SphI SspBI SspI StuI StyI SwaI Tru9I Tsp45I Tsp509I TspRI Tth111I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AhdI 433 GACAGCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AscI 624 GGCGCGCC GG^CGCGCC AspEI 433 GACAGCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BamHI 478 GGATCC G^GATCC BanI 580 GGCGCC G^G[CT][GA]CC BanII 116 GGGCTC G[GA]GC[CT]^C BbeI 580 GGCGCC GGCGC^C BfaI 332 CTAG C^TAG BmyI 116 GGGCTC G[AGT]GC[ACT]^C BsaI 426 GGTCTC GGTCTC(1/5) BsgI 17 CTGCAC GTGCAG(16/14) BsmAI 427 GTCTC GTCTC(1/5) BsmI 90 GCATTC GAATGC(1/-1) Bsp1286I 116 GGGCTC G[AGT]GC[ACT]^C BssSI 454 CTCGTG CACGAG(-5/-1) BstF5I 444 GGATG GGATG(2/0) DsaI 317 CCACGG C^C[GA][CT]GG Eam1105I 433 GACAGCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC FokI 444 GGATG GGATG(9/13) HaeII 580 GGCGCC [GA]GCGC^[CT] HphI 740 TCACC GGTGA(8/7) KasI 580 GGCGCC G^GCGCC MaeI 332 CTAG C^TAG NaeI 583 GCCGGC GCC^GGC NarI 580 GGCGCC GG^CGCC NdeI 356 CATATG CA^TATG NgoMIV 583 GCCGGC G^CCGGC NheI 331 GCTAGC G^CTAGC NspI 548 ACATGC [GA]CATG^[CT] SbfI 253 CCTGCAGG CCTGCA^GG SfoI 580 GGCGCC GGC^GCC SgrAI 582 CGCCGGCG C[GA]^CCGG[CT]G SmaI 291 CCCGGG CCC^GGG Sse8387I 253 CCTGCAGG CCTGCA^GG TfiI 106 GATTC G^A[AT]TC XmaCI 291 CCCGGG C^CCGGG XmaI 291 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AvaI 291 CCCGGG C^[CT]CG[GA]G 466 CCCGAG BseRI 600 CTCCTC GAGGAG(10/8) 388 GAGGAG BsoBI 291 CCCGGG C^[CT]CG[GA]G 466 CCCGAG BsrFI 583 GCCGGC [GA]^CCGG[CT] 709 ACCGGC BstYI 478 GGATCC [GA]^GATC[CT] 716 AGATCC Cfr10I 583 GCCGGC [GA]^CCGG[CT] 709 ACCGGC DpnII 479 GATC ^GATC 717 GATC FauI 307 CCCGC CCCGC(4/6) 542 GCGGG MboI 479 GATC ^GATC 717 GATC MflI 478 GGATCC [GA]^GATC[CT] 716 AGATCC NdeII 479 GATC ^GATC 717 GATC NlaIII 549 CATG CATG^ 663 CATG PflMI 336 CCAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 672 CCAGAAGCTGG PstI 254 CTGCAG CTGCA^G 770 CTGCAG PvuII 42 CAGCTG CAG^CTG 81 CAGCTG Sau3AI 479 GATC ^GATC 717 GATC Van91I 336 CCAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 672 CCAGAAGCTGG XhoII 478 GGATCC [GA]^GATC[CT] 716 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 56 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AlwNI BbsI BbvI BcnI BglI BpmI BpuAI BsaHI BsaJI BsiYI BslI BsmFI BsrI BssHII BssKI BstNI BstUI Cac8I CfoI Cfr13I DraII EcoO109I EcoRII Fnu4HI HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII ItaI MboII MnlI MspA1I MspI MvaI MvnI MwoI NciI NlaIV PleI RsaI Sau96I ScrFI SfcI TaqI TseI TthHB8I