(Circular) (Six-base) MAPSORT of: pFN21AB7544.seq Check: 1644 from: 1 to: 5491 pFN21AB7544 5491 bp With 182 enzymes: SgfI * December 28, 2011 15:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3301 278 Size: 53 83 186 936 1765 2468 Fragments arranged by size: 2468 1765 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2834 1937 Size: 897 4594 Acc65I G'GTAC_C Cuts at: 2812 2812 Size: 5491 AclI AA'CG_TT Cuts at: 1563 3618 3991 1563 Size: 2055 373 3063 Fragments arranged by size: 3063 2055 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2029 3554 4938 5206 1632 Size: 397 1525 1384 268 1917 Fragments arranged by size: 1917 1525 1384 397 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5294 AflIII A'CryG_T Cuts at: 1203 4390 5367 1203 Size: 3187 977 1327 Fragments arranged by size: 3187 1327 977 AgeI A'CCGG_T Cuts at: 4322 4322 Size: 5491 AhdI GACnn_n'nnGTC Cuts at: 2443 4220 2443 Size: 1777 3714 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5491 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2525 1184 Size: 1341 4150 AlwNI CAG_nnn'CTG Cuts at: 2347 2527 2683 3234 4806 2347 Size: 180 156 551 1572 3032 Fragments arranged by size: 3032 1572 551 180 156 ApaLI G'TGCA_C Cuts at: 3548 4704 3548 Size: 1156 4335 ApoI r'AATT_y Cuts at: 1493 2800 3026 3178 5287 1493 Size: 1307 226 152 2109 1697 Fragments arranged by size: 2109 1697 1307 226 152 AscI GG'CGCG_CC Cuts at: 2630 2630 Size: 5491 AseI AT'TA_AT Cuts at: 160 1032 4045 160 Size: 872 3013 1606 Fragments arranged by size: 3013 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5491 AvaI C'yCGr_G Cuts at: 1870 1943 2296 2471 2816 1870 Size: 73 353 175 345 4545 Fragments arranged by size: 4545 353 345 175 73 BamHI G'GATC_C Cuts at: 2483 2821 3188 2483 Size: 338 367 4786 Fragments arranged by size: 4786 367 338 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2585 2812 4267 618 Size: 325 217 627 798 227 1455 1842 Fragments arranged by size: 1842 1455 798 627 325 227 217 BanII G_rGCy'C Cuts at: 1391 2125 2810 1391 Size: 734 685 4072 Fragments arranged by size: 4072 734 685 BbeI G_GCGC'C Cuts at: 2589 2589 Size: 5491 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2062 2406 2587 928 Size: 971 163 344 181 3832 Fragments arranged by size: 3832 971 344 181 163 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3682 3716 1903 Size: 34 50 34 1661 34 3678 Fragments arranged by size: 3678 1661 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3383 4593 3383 Size: 1210 4281 BclI T'GATC_A Cuts at: 1548 1548 Size: 5491 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2018 2462 2640 Size: 107 122 71 631 605 346 444 178 Cuts at: 2640 4102 136 Size: 1462 1525 Fragments arranged by size: 1525 1462 631 605 444 346 178 122 107 71 BglII A'GATC_T Cuts at: 5486 5486 Size: 5491 BlpI GC'TnA_GC Cuts at: 2908 2908 Size: 5491 Bme1580I G_kGCm'C Cuts at: 1790 3552 4708 1790 Size: 1762 1156 2573 Fragments arranged by size: 2573 1762 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5491 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3222 4180 442 Size: 416 53 83 399 24 1805 958 1753 Fragments arranged by size: 1805 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2340 1056 Size: 1284 4207 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2036 2309 2447 4151 1239 Size: 797 273 138 1704 2579 Fragments arranged by size: 2579 1704 797 273 138 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3616 4481 4779 3616 Size: 865 298 4328 Fragments arranged by size: 4328 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2437 4154 882 Size: 617 938 1717 2219 Fragments arranged by size: 2219 1717 938 617 BsaAI yAC'GTr Cuts at: 493 1204 3282 493 Size: 711 2078 2702 Fragments arranged by size: 2702 2078 711 BsaBI GATnn'nnATC Cuts at: 5485 5485 Size: 5491 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2067 2586 2592 3298 Size: 53 83 186 936 534 519 6 706 Cuts at: 3298 3680 4315 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 706 635 534 519 382 186 83 53 6 BsaWI w'CCGG_w Cuts at: 3924 4322 4596 4743 5154 3924 Size: 398 274 147 411 4261 Fragments arranged by size: 4261 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5266 Fragments arranged by size: 5266 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2408 2596 1186 Size: 1222 188 4081 Fragments arranged by size: 4081 1222 188 BseYI C'CCAG_C Cuts at: 2164 2226 2282 2375 2530 2637 4694 5190 2164 Size: 62 56 93 155 107 2057 496 2465 Fragments arranged by size: 2465 2057 496 155 107 93 62 56 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2007 1163 Size: 573 271 4647 Fragments arranged by size: 4647 573 271 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2941 3702 4730 1540 Size: 275 186 940 761 1028 2301 Fragments arranged by size: 2301 1028 940 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3552 3637 4708 3552 Size: 85 1071 4335 Fragments arranged by size: 4335 1071 85 BsmI GAATG_Cn' Cuts at: 2095 3002 3095 2095 Size: 907 93 4491 Fragments arranged by size: 4491 907 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2125 2810 3552 3637 4708 1391 Size: 399 335 685 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 685 399 335 85 BspEI T'CCGG_A Cuts at: 5154 5154 Size: 5491 BspHI T'CATG_A Cuts at: 3380 5110 3380 Size: 1730 3761 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2846 844 Size: 2002 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3378 1394 Size: 429 1555 3507 Fragments arranged by size: 3507 1555 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3986 4160 65 Size: 1183 2738 174 1396 Fragments arranged by size: 2738 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2588 2714 4135 4322 1700 Size: 110 778 126 1421 187 2869 Fragments arranged by size: 2869 1421 778 187 126 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5491 BssHII G'CGCG_C Cuts at: 1925 2630 2687 2689 2763 1925 Size: 705 57 2 74 4653 Fragments arranged by size: 4653 705 74 57 2 BssSI C'ACGA_G Cuts at: 2459 3551 4563 2459 Size: 1092 1012 3387 Fragments arranged by size: 3387 1092 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5491 BstBI TT'CG_AA Cuts at: 4295 4295 Size: 5491 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5491 BstYI r'GATC_y Cuts at: 1972 2483 2721 2821 3188 3573 3590 5042 5128 Size: 511 238 100 367 385 17 1452 86 Cuts at: 5128 5140 5486 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 511 385 367 346 238 100 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2322 513 Size: 552 1257 3682 Fragments arranged by size: 3682 1257 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4752 Fragments arranged by size: 4752 683 56 BtsI GCAGTG_nn' Cuts at: 3007 3801 3821 3007 Size: 794 20 4677 Fragments arranged by size: 4677 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5491 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5456 DraI TTT'AAA Cuts at: 2795 2795 Size: 5491 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4498 809 Size: 507 3182 1802 Fragments arranged by size: 3182 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2938 3827 5456 Size: 54 1425 50 275 18 1108 889 1629 Cuts at: 5456 8 Size: 43 Fragments arranged by size: 1629 1425 1108 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2938 1537 Size: 275 1126 4090 Fragments arranged by size: 4090 1126 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3421 1353 Size: 2068 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2771 4078 4452 4598 387 Size: 1064 138 1182 1307 374 146 1280 Fragments arranged by size: 1307 1280 1182 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2029 2036 2309 2447 3554 4151 4938 Size: 393 397 7 273 138 1107 597 787 Cuts at: 4938 5206 1239 Size: 268 1524 Fragments arranged by size: 1524 1107 787 597 397 393 273 268 138 7 EcoO109I rG'GnC_Cy Cuts at: 2293 2300 2455 2293 Size: 7 155 5329 Fragments arranged by size: 5329 155 7 EcoRI G'AATT_C Cuts at: 2800 3178 2800 Size: 378 5113 FspI TGC'GCA Cuts at: 3997 3997 Size: 5491 HaeII r_GCGC'y Cuts at: 1678 2589 4638 1678 Size: 911 2049 2531 Fragments arranged by size: 2531 2049 911 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2429 2461 4132 4164 4206 Size: 32 135 32 857 32 1671 32 42 Cuts at: 4206 4238 1373 Size: 32 2626 Fragments arranged by size: 2626 1671 857 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2835 3081 669 Size: 988 281 897 246 3079 Fragments arranged by size: 3079 988 897 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5491 HpaI GTT'AAC Cuts at: 3081 3081 Size: 5491 KasI G'GCGC_C Cuts at: 2585 2585 Size: 5491 KpnI G_GTAC'C Cuts at: 2816 2816 Size: 5491 MfeI C'AATT_G Cuts at: 3090 3090 Size: 5491 MluI A'CGCG_T Cuts at: 5367 5367 Size: 5491 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2391 2427 4605 4789 1305 Size: 1086 36 2178 184 2007 Fragments arranged by size: 2178 2007 1086 184 36 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3669 Fragments arranged by size: 3669 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3449 3808 3967 518 Size: 648 2283 359 159 2042 Fragments arranged by size: 2283 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2049 2088 2637 2907 3584 4732 4977 1606 Size: 443 39 549 270 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 549 443 270 245 39 NaeI GCC'GGC Cuts at: 2590 2590 Size: 5491 NarI GG'CG_CC Cuts at: 2586 2586 Size: 5491 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4939 NdeI CA'TA_TG Cuts at: 387 2362 387 Size: 1975 3516 NgoMIV G'CCGG_C Cuts at: 2588 2588 Size: 5491 NheI G'CTAG_C Cuts at: 1052 2336 1052 Size: 1284 4207 NotI GC'GGCC_GC Cuts at: 2938 2938 Size: 5491 NspI r_CATG'y Cuts at: 1132 1229 2557 2849 4394 1132 Size: 97 1328 292 1545 2229 Fragments arranged by size: 2229 1545 1328 292 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5491 PciI A'CATG_T Cuts at: 4390 4390 Size: 5491 PflMI CCAn_nnn'nTGG Cuts at: 1108 2347 2683 1108 Size: 1239 336 3916 Fragments arranged by size: 3916 1239 336 PmeI GTTT'AAAC Cuts at: 2795 2795 Size: 5491 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3560 3592 5073 5105 3560 Size: 32 1481 32 3946 Fragments arranged by size: 3946 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5491 PsiI TTA'TAA Cuts at: 3061 3061 Size: 5491 PstI C_TGCA'G Cuts at: 830 2263 2779 2843 830 Size: 1433 516 64 3478 Fragments arranged by size: 3478 1433 516 64 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5491 PvuII CAG'CTG Cuts at: 2049 2088 2049 Size: 39 5452 SalI G'TCGA_C Cuts at: 1936 2833 1936 Size: 897 4594 SbfI CC_TGCA'GG Cuts at: 2263 2843 2263 Size: 580 4911 ScaI AGT'ACT Cuts at: 3739 5451 3739 Size: 1712 3779 SfcI C'TryA_G Cuts at: 826 1045 2251 2259 2775 2839 3974 4655 4846 Size: 219 1206 8 516 64 1135 681 191 Cuts at: 4846 826 Size: 1471 Fragments arranged by size: 1471 1206 1135 681 516 219 191 64 8 SfoI GGC'GCC Cuts at: 2587 2587 Size: 5491 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5491 SgrAI Cr'CCGG_yG Cuts at: 1700 2588 1700 Size: 888 4603 SmaI CCC'GGG Cuts at: 1872 2298 2818 1872 Size: 426 520 4545 Fragments arranged by size: 4545 520 426 SmlI C'TyrA_G Cuts at: 820 1017 1943 3595 4496 4758 820 Size: 197 926 1652 901 262 1553 Fragments arranged by size: 1652 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3282 493 Size: 2789 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5491 SphI G_CATG'C Cuts at: 1132 2849 1132 Size: 1717 3774 SspI AAT'ATT Cuts at: 5 52 3415 5 Size: 47 3363 2081 Fragments arranged by size: 3363 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5491 StyI C'CwwG_G Cuts at: 513 1065 2930 513 Size: 552 1865 3074 Fragments arranged by size: 3074 1865 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2563 3518 3535 3688 3873 4318 325 Size: 1255 983 955 17 153 185 445 1498 Fragments arranged by size: 1498 1255 983 955 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3737 5449 96 Size: 274 80 33 51 1443 1760 1712 138 Fragments arranged by size: 1760 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5451 5483 5451 Size: 32 5459 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5491 XbaI T'CTAG_A Cuts at: 2827 2827 Size: 5491 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2011 Size: 5491 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5491 XmaI C'CCGG_G Cuts at: 1870 2296 2816 1870 Size: 426 520 4545 Fragments arranged by size: 4545 520 426 XmnI GAAnn'nnTTC Cuts at: 3620 3620 Size: 5491 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3299 276 Size: 53 83 186 936 1765 2468 Fragments arranged by size: 2468 1765 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI