(Linear) (Six-base) MAPSORT of: KIBB7544.seq Check: 3811 from: 1 to: 786 KIBB7544.orf With 182 enzymes: SgfI * December 27, 2011 16:29 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 24 786 Size: 24 762 AhdI GACnn_n'nnGTC Cuts at: 0 438 786 Size: 438 348 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 520 786 Size: 520 266 AlwNI CAG_nnn'CTG Cuts at: 0 342 522 678 786 Size: 342 180 156 108 Fragments arranged by size: 342 180 156 108 AscI GG'CGCG_CC Cuts at: 0 625 786 Size: 625 161 AvaI C'yCGr_G Cuts at: 0 291 466 786 Size: 291 175 320 Fragments arranged by size: 320 291 175 BamHI G'GATC_C Cuts at: 0 478 786 Size: 478 308 BanI G'GyrC_C Cuts at: 0 580 786 Size: 580 206 BanII G_rGCy'C Cuts at: 0 120 786 Size: 120 666 BbeI G_GCGC'C Cuts at: 0 584 786 Size: 584 202 BbsI GAAGACnn'nnnn_ Cuts at: 0 57 401 582 786 Size: 57 344 181 204 Fragments arranged by size: 344 204 181 57 BglI GCCn_nnn'nGGC Cuts at: 0 13 457 635 786 Size: 13 444 178 151 Fragments arranged by size: 444 178 151 13 BmtI G_CTAG'C Cuts at: 0 335 786 Size: 335 451 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 31 304 442 786 Size: 31 273 138 344 Fragments arranged by size: 344 273 138 31 BsaI GGTCTCn'nnnn_ Cuts at: 0 432 786 Size: 432 354 BsaHI Gr'CG_yC Cuts at: 0 62 581 587 786 Size: 62 519 6 199 Fragments arranged by size: 519 199 62 6 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 403 591 786 Size: 403 188 195 Fragments arranged by size: 403 195 188 BseYI C'CCAG_C Cuts at: 0 159 221 277 370 525 632 786 Size: 159 62 56 93 155 107 154 Fragments arranged by size: 159 155 154 107 93 62 56 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2 786 Size: 2 784 BsmI GAATG_Cn' Cuts at: 0 90 786 Size: 90 696 Bsp1286I G_dGCh'C Cuts at: 0 120 786 Size: 120 666 BsrFI r'CCGG_y Cuts at: 0 583 709 786 Size: 583 126 77 Fragments arranged by size: 583 126 77 BssHII G'CGCG_C Cuts at: 0 625 682 684 758 786 Size: 625 57 2 74 28 Fragments arranged by size: 625 74 57 28 2 BssSI C'ACGA_G Cuts at: 0 454 786 Size: 454 332 BstYI r'GATC_y Cuts at: 0 478 716 786 Size: 478 238 70 Fragments arranged by size: 478 238 70 BtgI C'CryG_G Cuts at: 0 317 786 Size: 317 469 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 766 786 Size: 766 20 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 24 31 304 442 786 Size: 24 7 273 138 344 Fragments arranged by size: 344 273 138 24 7 EcoO109I rG'GnC_Cy Cuts at: 0 288 295 450 786 Size: 288 7 155 336 Fragments arranged by size: 336 288 155 7 HaeII r_GCGC'y Cuts at: 0 584 786 Size: 584 202 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 424 456 786 Size: 424 32 330 Fragments arranged by size: 424 330 32 KasI G'GCGC_C Cuts at: 0 580 786 Size: 580 206 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 386 422 786 Size: 386 36 364 Fragments arranged by size: 386 364 36 MspA1I CmG'CkG Cuts at: 0 44 83 632 786 Size: 44 39 549 154 Fragments arranged by size: 549 154 44 39 NaeI GCC'GGC Cuts at: 0 585 786 Size: 585 201 NarI GG'CG_CC Cuts at: 0 581 786 Size: 581 205 NdeI CA'TA_TG Cuts at: 0 357 786 Size: 357 429 NgoMIV G'CCGG_C Cuts at: 0 583 786 Size: 583 203 NheI G'CTAG_C Cuts at: 0 331 786 Size: 331 455 NspI r_CATG'y Cuts at: 0 552 786 Size: 552 234 PflMI CCAn_nnn'nTGG Cuts at: 0 342 678 786 Size: 342 336 108 Fragments arranged by size: 342 336 108 PstI C_TGCA'G Cuts at: 0 258 774 786 Size: 258 516 12 Fragments arranged by size: 516 258 12 PvuII CAG'CTG Cuts at: 0 44 83 786 Size: 44 39 703 Fragments arranged by size: 703 44 39 SbfI CC_TGCA'GG Cuts at: 0 258 786 Size: 258 528 SfcI C'TryA_G Cuts at: 0 246 254 770 786 Size: 246 8 516 16 Fragments arranged by size: 516 246 16 8 SfoI GGC'GCC Cuts at: 0 582 786 Size: 582 204 SgrAI Cr'CCGG_yG Cuts at: 0 583 786 Size: 583 203 SmaI CCC'GGG Cuts at: 0 293 786 Size: 293 493 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 558 786 Size: 558 228 XmaI C'CCGG_G Cuts at: 0 291 786 Size: 291 495 Enzymes that do cut: AcuI AhdI AlfI AlwNI AscI AvaI BamHI BanI BanII BbeI BbsI BglI BmtI BpmI BsaI BsaHI BseRI BseYI BsgI BsmI Bsp1286I BsrFI BssHII BssSI BstYI BtgI EciI Eco57MI EcoO109I HaeII Hin4I KasI MmeI MspA1I NaeI NarI NdeI NgoMIV NheI NspI PflMI PstI PvuII SbfI SfcI SfoI SgrAI SmaI TaqII XmaI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AleI AloI ApaI ApaLI ApoI AseI AsiSI AvrII BaeI BbvCI BcgI BciVI BclI BfrBI BglII BlpI Bme1580I BmgBI BmrI BplI Bpu10I BpuEI BsaAI BsaBI BsaWI BsaXI BsiEI BsiHKAI BsiWI BsmBI BspEI BspHI BspMI BsrBI BsrDI BsrGI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KpnI MfeI MluI MscI MslI NcoI NotI NruI NsiI PacI PasI PciI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SapI ScaI SexAI SfiI SgfI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI SwaI TatI TstI Tth111I XbaI XcmI XhoI XmnI ZraI