Restriction map for
pFN21AE2727
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# NO CUT ENZYMES ( 63 enzymes) #
##################################
AatII AccI AclI AflII Alw44I
Aor51HI ApaLI AviII AvrII BbrPI
BcgI BclI BfrI BlnI BsaAI
BsiWI BsmI Bsp1407I BspDI BspHI
BspLU11I BsrGI Bst1107I BstBI BstZ17I
ClaI DraI DraIII Eco47III EcoRV
EcoT22I FbaI FspI HpaI KspI
MluI NdeI NheI NotI NruI
NsiI NspV PacI PmaCI PmeI
PmlI PshAI Psp1406I PvuI RcaI
SacII SalI SapI ScaI SfiI
SfuI SnaBI SpeI SphI SspBI
SspI SwaI XbaI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AccIII 581 TCCGGA T^CCGGA
AflIII 2786 ACGTGT A^C[GA][CT]GT
AgeI 632 ACCGGT A^CCGGT
AhdI 1432 GACCAGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 2853 CCTGAGG CC^T[ACGT]AGG
AscI 2329 GGCGCGCC GG^CGCGCC
AseI 2188 ATTAAT AT^TAAT
AsnI 2188 ATTAAT AT^TAAT
AspEI 1432 GACCAGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 655 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BciVI 2882 GTATCC GTATCC(6/5)
BlpI 880 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 880 GCTCAGC GC^T[ACGT]AGC
BsaBI 2757 GATCGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 581 TCCGGA T^CCGGA
BsmBI 3033 CGTCTC CGTCTC(1/5)
BspEI 581 TCCGGA T^CCGGA
BstAPI 402 GCAGGACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 2853 CCTGAGG CC^T[ACGT]AGG
CelII 880 GCTCAGC GC^T[ACGT]AGC
CpoI 121 CGGACCG CG^G[AT]CCG
Eam1105I 1432 GACCAGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2853 CCTGAGG CC^T[ACGT]AGG
FseI 246 GGCCGGCC GGCCGG^CC
HindIII 1277 AAGCTT A^AGCTT
MamI 2757 GATCGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 3271 CAATTG C^AATTG
MroI 581 TCCGGA T^CCGGA
MseI 2189 TTAA T^TAA
MunI 3271 CAATTG C^AATTG
PaeR7I 3316 CTCGAG C^TCGAG
PflFI 655 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 632 ACCGGT A^CCGGT
PshBI 2188 ATTAAT AT^TAAT
RsrII 121 CGGACCG CG^G[AT]CCG
SbfI 1038 CCTGCAGG CCTGCA^GG
SexAI 347 ACCTGGT A^CC[AT]GGT
Sse8387I 1038 CCTGCAGG CCTGCA^GG
Tru9I 2189 TTAA T^TAA
Tth111I 655 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 3316 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 28 enzymes) #
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Acc65I 629 GGTACC G^GTACC
1553 GGTACC
AcsI 1114 GAATTC [GA]^AATT[CT]
3202 GAATTC
ApoI 1114 GAATTC [GA]^AATT[CT]
3202 GAATTC
Asp700I 738 GAACCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1234 GAACTCTTTC
Asp718I 629 GGTACC G^GTACC
1553 GGTACC
BamHI 29 GGATCC G^GATCC
291 GGATCC
BglII 38 AGATCT A^GATCT
2918 AGATCT
BsaI 937 GAGACC GGTCTC(1/5)
1120 GAGACC
BsaWI 581 TCCGGA [AT]^CCGG[AT]
632 ACCGGT
BsiEI 249 CGGCCG CG[GA][CT]^CG
486 CGGCCG
BsrDI 1221 CATTGC GCAATG(2/0)
3117 CATTGC
BssHII 1544 GCGCGC G^CGCGC
2330 GCGCGC
DrdI 808 GACATCCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1027 GACCTTGAGGTC
EagI 249 CGGCCG C^GGCCG
486 CGGCCG
EarI 2800 CTCTTC CTCTTC(1/4)
2140 GAAGAG
EclXI 249 CGGCCG C^GGCCG
486 CGGCCG
Eco52I 249 CGGCCG C^GGCCG
486 CGGCCG
EcoRI 1114 GAATTC G^AATTC
3202 GAATTC
KpnI 629 GGTACC GGTAC^C
1553 GGTACC
Ksp632I 2800 CTCTTC CTCTTC(1/4)
2140 GAAGAG
NcoI 143 CCATGG C^CATGG
554 CCATGG
SgrAI 2296 CGCCGGTG C[GA]^CCGG[CT]G
2333 CGCCGGTG
SmaI 2533 CCCGGG CCC^GGG
2652 CCCGGG
SmlI 1030 CTTGAG C^T[CT][GA]AG
3316 CTCGAG
StuI 1148 AGGCCT AGG^CCT
1166 AGGCCT
XmaCI 2533 CCCGGG C^CCGGG
2652 CCCGGG
XmaI 2533 CCCGGG C^CCGGG
2652 CCCGGG
XmnI 738 GAACCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1234 GAACTCTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 126 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII BalI BanI BanII BbeI
BbsI BbvCI BbvI BcnI BfaI
BglI BmyI BpmI BpuAI BsaHI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BssSI BstEII BstF5I BstNI BstPI
BstUI BstXI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EcoNI EcoO109I EcoO65I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI KasI MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NarI NciI NdeII NgoMIV NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
StyI TaqI TfiI TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII