(Circular) (Six-base) MAPSORT of: pFN21AE2727.seq Check: 5989 from: 1 to: 8347 pFN21AE2727 8347 bp With 182 enzymes: SgfI * August 16, 2021 15:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4631 5042 4631 Size: 411 7936 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6157 278 Size: 53 83 186 936 4621 2468 Fragments arranged by size: 4621 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5690 1937 Size: 3753 4594 Acc65I G'GTAC_C Cuts at: 2634 3558 5668 2634 Size: 924 2110 5313 Fragments arranged by size: 5313 2110 924 AclI AA'CG_TT Cuts at: 1563 6474 6847 1563 Size: 4911 373 3063 Fragments arranged by size: 4911 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4117 5468 6410 7794 8062 1632 Size: 2485 1351 942 1384 268 1917 Fragments arranged by size: 2485 1917 1384 1351 942 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8150 AflIII A'CryG_T Cuts at: 1203 4791 7246 8223 1203 Size: 3588 2455 977 1327 Fragments arranged by size: 3588 2455 1327 977 AgeI A'CCGG_T Cuts at: 2637 7178 2637 Size: 4541 3806 AhdI GACnn_n'nnGTC Cuts at: 3442 7076 3442 Size: 3634 4713 AleI CACnn'nnGTG Cuts at: 1166 2549 4039 1166 Size: 1383 1490 5474 Fragments arranged by size: 5474 1490 1383 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3635 1184 Size: 2451 5896 AlwNI CAG_nnn'CTG Cuts at: 2413 4027 4501 4570 4980 5518 6090 7662 2413 Size: 1614 474 69 410 538 572 1572 3098 Fragments arranged by size: 3098 1614 1572 572 538 474 410 69 ApaI G_GGCC'C Cuts at: 2387 4081 4538 4758 5023 2387 Size: 1694 457 220 265 5711 Fragments arranged by size: 5711 1694 457 265 220 ApaLI G'TGCA_C Cuts at: 6404 7560 6404 Size: 1156 7191 ApoI r'AATT_y Cuts at: 1493 3119 5207 5656 5882 6034 8143 1493 Size: 1626 2088 449 226 152 2109 1697 Fragments arranged by size: 2109 2088 1697 1626 449 226 152 AscI GG'CGCG_CC Cuts at: 4335 4335 Size: 8347 AseI AT'TA_AT Cuts at: 160 1032 4194 6901 160 Size: 872 3162 2707 1606 Fragments arranged by size: 3162 2707 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8347 AvaI C'yCGr_G Cuts at: 1870 1943 2997 3665 4538 4657 5321 5672 1870 Size: 73 1054 668 873 119 664 351 4545 Fragments arranged by size: 4545 1054 873 668 664 351 119 73 BamHI G'GATC_C Cuts at: 2034 2296 5677 6044 2034 Size: 262 3381 367 4337 Fragments arranged by size: 4337 3381 367 262 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2011 2118 2543 2634 3403 Size: 325 217 627 224 107 425 91 769 Cuts at: 3403 3458 3558 3572 4299 4305 4426 5030 5668 Size: 55 100 14 727 6 121 604 638 Cuts at: 5668 7123 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 769 727 638 627 604 425 325 224 217 121 107 100 91 55 14 6 BanII G_rGCy'C Cuts at: 1391 2387 2621 3084 3167 3498 4081 4155 4538 Size: 996 234 463 83 331 583 74 383 Cuts at: 4538 4758 4857 4995 5023 5300 5666 1391 Size: 220 99 138 28 277 366 4072 Fragments arranged by size: 4072 996 583 463 383 366 331 277 234 220 138 99 83 74 28 BbeI G_GCGC'C Cuts at: 2122 3407 3576 4303 4430 2122 Size: 1285 169 727 127 6039 Fragments arranged by size: 6039 1285 727 169 127 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2063 2792 2958 4241 4894 928 Size: 971 164 729 166 1283 653 4381 Fragments arranged by size: 4381 1283 971 729 653 166 164 BbvCI CC'TCA_GC Cuts at: 3175 3350 3524 3766 3822 3175 Size: 175 174 242 56 7700 Fragments arranged by size: 7700 242 175 174 56 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6538 6572 1903 Size: 34 4601 34 3678 Fragments arranged by size: 4601 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4898 6239 7449 4898 Size: 1341 1210 5796 Fragments arranged by size: 5796 1341 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 8347 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2165 2736 2882 Size: 107 122 71 631 605 493 571 146 Cuts at: 2882 4263 6958 136 Size: 1381 2695 1525 Fragments arranged by size: 2695 1525 1381 631 605 571 493 146 122 107 71 BglII A'GATC_T Cuts at: 2043 4923 8342 2043 Size: 2880 3419 2048 Fragments arranged by size: 3419 2880 2048 BlpI GC'TnA_GC Cuts at: 2886 5764 2886 Size: 2878 5469 Bme1580I G_kGCm'C Cuts at: 1790 2347 2387 2546 3703 3721 4081 4538 4758 Size: 557 40 159 1157 18 360 457 220 Cuts at: 4758 5008 5023 5033 6408 7564 1790 Size: 250 15 10 1375 1156 2573 Fragments arranged by size: 2573 1375 1157 1156 557 457 360 250 220 159 40 18 15 10 BmgBI CAC'GTC Cuts at: 1318 3511 4792 4957 5457 1318 Size: 2193 1281 165 500 4208 Fragments arranged by size: 4208 2193 1281 500 165 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2478 2547 5038 Size: 416 53 83 399 24 1061 69 2491 Cuts at: 5038 6078 7036 442 Size: 1040 958 1753 Fragments arranged by size: 2491 1753 1061 1040 958 416 399 83 69 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8347 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2618 5452 2618 Size: 2834 5513 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2378 2783 3700 3830 3953 4463 5549 7007 Size: 1139 405 917 130 123 510 1086 1458 Cuts at: 7007 1239 Size: 2579 Fragments arranged by size: 2579 1458 1139 1086 917 510 405 130 123 Bpu10I CC'TnA_GC Cuts at: 2914 3175 3350 3524 3766 3822 4502 5270 5582 Size: 261 175 174 242 56 680 768 312 Cuts at: 5582 2914 Size: 5679 Fragments arranged by size: 5679 768 680 312 261 242 175 174 56 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3056 6472 7337 7635 3056 Size: 3416 865 298 3768 Fragments arranged by size: 3768 3416 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2936 3119 7010 882 Size: 617 1437 183 3891 2219 Fragments arranged by size: 3891 2219 1437 617 183 BsaAI yAC'GTr Cuts at: 493 1204 6138 493 Size: 711 4934 2702 Fragments arranged by size: 4934 2702 711 BsaBI GATnn'nnATC Cuts at: 4766 8341 4766 Size: 3575 4772 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2119 3297 3404 3443 Size: 53 83 186 936 586 1178 107 39 Cuts at: 3443 3573 3990 4238 4300 4427 6154 6536 7171 Size: 130 417 248 62 127 1727 382 635 Cuts at: 7171 275 Size: 1451 Fragments arranged by size: 1727 1451 1178 936 635 586 417 382 248 186 130 127 107 83 62 53 39 BsaWI w'CCGG_w Cuts at: 2586 2637 6780 7178 7452 7599 8010 2586 Size: 51 4143 398 274 147 411 2923 Fragments arranged by size: 4143 2923 411 398 274 147 51 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3599 3629 4157 4187 1178 Size: 30 165 30 2196 30 528 30 5338 Fragments arranged by size: 5338 2196 528 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2819 2891 3839 3917 3920 4163 4178 5063 Size: 1633 72 948 78 3 243 15 885 Cuts at: 5063 1186 Size: 4470 Fragments arranged by size: 4470 1633 948 885 243 78 72 15 3 BseYI C'CCAG_C Cuts at: 2368 3469 3535 4225 4243 4750 5301 5488 7550 Size: 1101 66 690 18 507 551 187 2062 Cuts at: 7550 8046 2368 Size: 496 2669 Fragments arranged by size: 2669 2062 1101 690 551 507 496 187 66 18 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2047 2462 5302 1163 Size: 573 311 415 2840 4208 Fragments arranged by size: 4208 2840 573 415 311 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2257 2494 5797 6558 7586 1540 Size: 275 186 256 237 3303 761 1028 2301 Fragments arranged by size: 3303 2301 1028 761 275 256 237 186 BsiHKAI G_wGCw'C Cuts at: 2450 3084 3167 3498 4974 6408 6493 7564 2450 Size: 634 83 331 1476 1434 85 1071 3233 Fragments arranged by size: 3233 1476 1434 1071 634 331 85 83 BsmI GAATG_Cn' Cuts at: 5858 5951 5858 Size: 93 8254 BsmBI CGTCTCn'nnnn_ Cuts at: 5044 5044 Size: 8347 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2347 2387 2450 2546 2621 3084 3167 Size: 399 557 40 63 96 75 463 83 Cuts at: 3167 3498 3703 3721 4081 4155 4538 4758 4857 Size: 331 205 18 360 74 383 220 99 Cuts at: 4857 4974 4995 5008 5023 5033 5300 5666 6408 Size: 117 21 13 15 10 267 366 742 Cuts at: 6408 6493 7564 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 557 463 399 383 366 360 331 267 220 205 117 99 96 85 83 75 74 63 40 21 18 15 13 10 BspEI T'CCGG_A Cuts at: 2586 8010 2586 Size: 5424 2923 BspHI T'CATG_A Cuts at: 6236 7966 6236 Size: 1730 6617 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2069 2421 3696 4383 4631 4926 5042 5637 Size: 1225 352 1275 687 248 295 116 595 Cuts at: 5637 5702 844 Size: 65 3489 Fragments arranged by size: 3489 1275 1225 687 595 352 295 248 116 65 BsrBI CCG'CTC Cuts at: 1394 1823 3646 3946 4506 5359 5533 5589 6234 Size: 429 1823 300 560 853 174 56 645 Cuts at: 6234 1394 Size: 3507 Fragments arranged by size: 3507 1823 853 645 560 429 300 174 56 BsrDI GCAATG_nn' Cuts at: 65 1248 3225 5121 6842 7016 65 Size: 1183 1977 1896 1721 174 1396 Fragments arranged by size: 1977 1896 1721 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2252 2493 2637 3312 4302 4339 4423 Size: 110 442 241 144 675 990 37 84 Cuts at: 4423 4647 6991 7178 1700 Size: 224 2344 187 2869 Fragments arranged by size: 2869 2344 990 675 442 241 224 187 144 110 84 37 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8347 BssHII G'CGCG_C Cuts at: 1925 3549 4335 1925 Size: 1624 786 5937 Fragments arranged by size: 5937 1624 786 BssSI C'ACGA_G Cuts at: 2903 4409 5090 6407 7419 2903 Size: 1506 681 1317 1012 3831 Fragments arranged by size: 3831 1506 1317 1012 681 BstAPI GCAn_nnn'nTGC Cuts at: 794 2413 794 Size: 1619 6728 BstBI TT'CG_AA Cuts at: 7151 7151 Size: 8347 BstEII G'GTnAC_C Cuts at: 1184 2176 2648 4342 1184 Size: 992 472 1694 5189 Fragments arranged by size: 5189 1694 992 472 BstXI CCAn_nnnn'nTGG Cuts at: 2554 2566 4354 5059 2554 Size: 12 1788 705 5842 Fragments arranged by size: 5842 1788 705 12 BstYI r'GATC_y Cuts at: 1972 2034 2043 2089 2296 4923 5677 6044 6429 Size: 62 9 46 207 2627 754 367 385 Cuts at: 6429 6446 7898 7984 7996 8342 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 2627 1977 1452 754 385 367 346 207 86 62 46 17 12 9 Bsu36I CC'TnA_GG Cuts at: 4859 4859 Size: 8347 BtgI C'CryG_G Cuts at: 513 1065 2004 2148 2172 2205 2559 3343 4845 Size: 552 939 144 24 33 354 784 1502 Cuts at: 4845 4865 513 Size: 20 3995 Fragments arranged by size: 3995 1502 939 784 552 354 144 33 24 20 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2208 487 Size: 683 56 982 6626 Fragments arranged by size: 6626 982 683 56 BtsI GCAGTG_nn' Cuts at: 2148 3910 5552 5863 6657 6677 2148 Size: 1762 1642 311 794 20 3818 Fragments arranged by size: 3818 1762 1642 794 311 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8347 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8312 DraI TTT'AAA Cuts at: 5651 5651 Size: 8347 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2819 3038 7354 809 Size: 507 1503 219 4316 1802 Fragments arranged by size: 4316 1802 1503 507 219 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2145 2254 2491 Size: 54 1425 50 275 18 315 109 237 Cuts at: 2491 2529 2556 3726 3975 4056 4546 4842 5094 Size: 38 27 1170 249 81 490 296 252 Cuts at: 5094 5571 5794 6683 8312 8 Size: 477 223 889 1629 43 Fragments arranged by size: 1629 1425 1170 889 490 477 315 296 275 252 249 237 223 109 81 54 50 43 38 27 18 EagI C'GGCC_G Cuts at: 1537 1812 2254 2491 5794 1537 Size: 275 442 237 3303 4090 Fragments arranged by size: 4090 3303 442 275 237 EarI CTCTTCn'nnn_ Cuts at: 1353 4140 4811 6277 1353 Size: 2787 671 1466 3423 Fragments arranged by size: 3423 2787 1466 671 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2954 4235 6934 7308 7454 387 Size: 1064 138 1365 1281 2699 374 146 1280 Fragments arranged by size: 2699 1365 1281 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3082 3165 3496 3082 Size: 83 331 7933 Fragments arranged by size: 7933 331 83 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2378 2783 3700 3830 3953 4117 4463 Size: 393 746 405 917 130 123 164 346 Cuts at: 4463 5468 5549 6410 7007 7794 8062 1239 Size: 1005 81 861 597 787 268 1524 Fragments arranged by size: 1524 1005 917 861 787 746 597 405 393 346 268 164 130 123 81 EcoNI CCTnn'n_nnAGG Cuts at: 3149 3588 3668 5345 3149 Size: 439 80 1677 6151 Fragments arranged by size: 6151 1677 439 80 EcoO109I rG'GnC_Cy Cuts at: 2100 2383 2384 2410 3031 3040 3142 3580 3673 Size: 283 1 26 621 9 102 438 93 Cuts at: 3673 3777 4078 4205 4249 4498 4534 4535 4754 Size: 104 301 127 44 249 36 1 219 Cuts at: 4754 5019 5578 2100 Size: 265 559 4869 Fragments arranged by size: 4869 621 559 438 301 283 265 249 219 127 104 102 93 44 36 26 9 1 1 EcoRI G'AATT_C Cuts at: 3119 5207 5656 6034 3119 Size: 2088 449 378 5432 Fragments arranged by size: 5432 2088 449 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3050 5413 5488 3050 Size: 2363 75 5909 Fragments arranged by size: 5909 2363 75 FseI GG_CCGG'CC Cuts at: 2256 2256 Size: 8347 FspI TGC'GCA Cuts at: 6853 6853 Size: 8347 HaeII r_GCGC'y Cuts at: 1678 2122 3407 3576 4303 4430 4570 5518 7494 Size: 444 1285 169 727 127 140 948 1976 Cuts at: 7494 1678 Size: 2531 Fragments arranged by size: 2531 1976 1285 948 727 444 169 140 127 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2991 3023 3095 3127 3428 Size: 32 135 32 1419 32 72 32 301 Cuts at: 3428 3460 3601 3633 6988 7020 7062 7094 1373 Size: 32 141 32 3355 32 42 32 2626 Fragments arranged by size: 3355 2626 1419 301 141 135 72 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2080 2859 3877 4741 5113 5691 Size: 988 281 142 779 1018 864 372 578 Cuts at: 5691 5937 669 Size: 246 3079 Fragments arranged by size: 3079 1018 988 864 779 578 372 281 246 142 HindIII A'AGCT_T Cuts at: 748 3282 748 Size: 2534 5813 HpaI GTT'AAC Cuts at: 5937 5937 Size: 8347 KasI G'GCGC_C Cuts at: 2118 3403 3572 4299 4426 2118 Size: 1285 169 727 127 6039 Fragments arranged by size: 6039 1285 727 169 127 KpnI G_GTAC'C Cuts at: 2638 3562 5672 2638 Size: 924 2110 5313 Fragments arranged by size: 5313 2110 924 MfeI C'AATT_G Cuts at: 5276 5946 5276 Size: 670 7677 MluI A'CGCG_T Cuts at: 8223 8223 Size: 8347 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3435 4968 7461 7645 1305 Size: 2130 1533 2493 184 2007 Fragments arranged by size: 2493 2130 2007 1533 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2147 2531 2558 3728 3977 Size: 54 1425 343 315 384 27 1170 249 Cuts at: 3977 4058 4548 4844 5096 10 Size: 81 490 296 252 3261 Fragments arranged by size: 3261 1425 1170 490 384 343 315 296 252 249 81 54 27 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2329 2502 2549 2564 4039 4352 Size: 648 843 320 173 47 15 1475 313 Cuts at: 4352 4955 5126 6305 6664 6823 518 Size: 603 171 1179 359 159 2042 Fragments arranged by size: 2042 1475 1179 843 648 603 359 320 313 173 171 159 47 15 MspA1I CmG'CkG Cuts at: 1606 2303 3973 4243 4290 4317 4530 4593 4750 Size: 697 1670 270 47 27 213 63 157 Cuts at: 4750 5488 5763 6440 7588 7833 1606 Size: 738 275 677 1148 245 2120 Fragments arranged by size: 2120 1670 1148 738 697 677 275 270 245 213 157 63 47 27 NaeI GCC'GGC Cuts at: 2254 2495 4425 4649 2254 Size: 241 1930 224 5952 Fragments arranged by size: 5952 1930 241 224 NarI GG'CG_CC Cuts at: 2119 3404 3573 4300 4427 2119 Size: 1285 169 727 127 6039 Fragments arranged by size: 6039 1285 727 169 127 NcoI C'CATG_G Cuts at: 513 1065 2004 2148 2559 513 Size: 552 939 144 411 6301 Fragments arranged by size: 6301 939 552 411 144 NdeI CA'TA_TG Cuts at: 387 387 Size: 8347 NgoMIV G'CCGG_C Cuts at: 2252 2493 4423 4647 2252 Size: 241 1930 224 5952 Fragments arranged by size: 5952 1930 241 224 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8347 NotI GC'GGCC_GC Cuts at: 5794 5794 Size: 8347 NspI r_CATG'y Cuts at: 1132 1229 2501 3521 3616 5705 7250 1132 Size: 97 1272 1020 95 2089 1545 2229 Fragments arranged by size: 2229 2089 1545 1272 1020 97 95 PasI CC'CwG_GG Cuts at: 1348 2574 2966 1348 Size: 1226 392 6729 Fragments arranged by size: 6729 1226 392 PciI A'CATG_T Cuts at: 7246 7246 Size: 8347 PflMI CCAn_nnn'nTGG Cuts at: 1108 2527 2972 5467 1108 Size: 1419 445 2495 3988 Fragments arranged by size: 3988 2495 1419 445 PfoI T'CCnGG_A Cuts at: 2092 5202 2092 Size: 3110 5237 PmeI GTTT'AAAC Cuts at: 5651 5651 Size: 8347 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6416 6448 7929 7961 6416 Size: 32 1481 32 6802 Fragments arranged by size: 6802 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2100 2410 3031 3040 3580 4249 2100 Size: 310 621 9 540 669 6198 Fragments arranged by size: 6198 669 621 540 310 9 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8347 PsiI TTA'TAA Cuts at: 5917 5917 Size: 8347 PspOMI G'GGCC_C Cuts at: 2383 4077 4534 4754 5019 2383 Size: 1694 457 220 265 5711 Fragments arranged by size: 5711 1694 457 265 220 PspXI vC'TCGA_Gb Cuts at: 5321 5321 Size: 8347 PstI C_TGCA'G Cuts at: 830 2308 2409 3048 4923 5699 830 Size: 1478 101 639 1875 776 3478 Fragments arranged by size: 3478 1875 1478 776 639 101 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8347 PvuII CAG'CTG Cuts at: 2303 3973 4593 4750 5488 2303 Size: 1670 620 157 738 5162 Fragments arranged by size: 5162 1670 738 620 157 RsrII CG'GwC_CG Cuts at: 2127 2127 Size: 8347 SacI G_AGCT'C Cuts at: 3084 3167 3498 3084 Size: 83 331 7933 Fragments arranged by size: 7933 331 83 SalI G'TCGA_C Cuts at: 1936 5689 1936 Size: 3753 4594 SanDI GG'GwC_CC Cuts at: 3580 4249 3580 Size: 669 7678 SbfI CC_TGCA'GG Cuts at: 3048 5699 3048 Size: 2651 5696 ScaI AGT'ACT Cuts at: 6595 8307 6595 Size: 1712 6635 SexAI A'CCwGG_T Cuts at: 2352 2352 Size: 8347 SfcI C'TryA_G Cuts at: 826 1045 2304 2405 3044 3394 4919 5695 6830 Size: 219 1259 101 639 350 1525 776 1135 Cuts at: 6830 7511 7702 826 Size: 681 191 1471 Fragments arranged by size: 1525 1471 1259 1135 776 681 639 350 219 191 101 SfoI GGC'GCC Cuts at: 2120 3405 3574 4301 4428 2120 Size: 1285 169 727 127 6039 Fragments arranged by size: 6039 1285 727 169 127 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8347 SgrAI Cr'CCGG_yG Cuts at: 1700 4302 4339 1700 Size: 2602 37 5708 Fragments arranged by size: 5708 2602 37 SmaI CCC'GGG Cuts at: 1872 4540 4659 5674 1872 Size: 2668 119 1015 4545 Fragments arranged by size: 4545 2668 1015 119 SmlI C'TyrA_G Cuts at: 820 1017 1943 3035 5321 6451 7352 7614 820 Size: 197 926 1092 2286 1130 901 262 1553 Fragments arranged by size: 2286 1553 1130 1092 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 6138 493 Size: 5645 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8347 SphI G_CATG'C Cuts at: 1132 5705 1132 Size: 4573 3774 SspI AAT'ATT Cuts at: 5 52 6271 5 Size: 47 6219 2081 Fragments arranged by size: 6219 2081 47 StuI AGG'CCT Cuts at: 1517 3155 3173 1517 Size: 1638 18 6691 Fragments arranged by size: 6691 1638 18 StyI C'CwwG_G Cuts at: 513 1065 2004 2148 2547 2559 2613 5786 513 Size: 552 939 144 399 12 54 3173 3074 Fragments arranged by size: 3173 3074 939 552 399 144 54 12 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2304 2552 2894 3603 4084 5557 6374 Size: 1255 724 248 342 709 481 1473 817 Cuts at: 6374 6391 6544 6729 7174 325 Size: 17 153 185 445 1498 Fragments arranged by size: 1498 1473 1255 817 724 709 481 445 342 248 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3732 5152 6593 Size: 274 80 33 51 1443 1755 1420 1441 Cuts at: 6593 8305 96 Size: 1712 138 Fragments arranged by size: 1755 1712 1443 1441 1420 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 8307 8339 8307 Size: 32 8315 Tth111I GACn'n_nGTC Cuts at: 1316 2663 1316 Size: 1347 7000 XbaI T'CTAG_A Cuts at: 5683 5683 Size: 8347 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4078 5059 5104 4078 Size: 981 45 7321 Fragments arranged by size: 7321 981 45 XhoI C'TCGA_G Cuts at: 1943 5321 1943 Size: 3378 4969 XmaI C'CCGG_G Cuts at: 1870 4538 4657 5672 1870 Size: 2668 119 1015 4545 Fragments arranged by size: 4545 2668 1015 119 XmnI GAAnn'nnTTC Cuts at: 2747 3243 6476 2747 Size: 496 3233 4618 Fragments arranged by size: 4618 3233 496 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6155 276 Size: 53 83 186 936 4621 2468 Fragments arranged by size: 4621 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII RsrII SacI SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AvrII BaeI BfrBI BsiWI BstZ17I DraIII EcoRV FspAI NruI NsiI PacI PmlI PsrI SacII SapI SfiI SrfI SwaI