Restriction map for
pFN21AE2727
(Linear) (Six-base) MAPSORT of: KIEE2727.seq Check: 6955 from: 1 to: 3642
KIEE2727.orf
With 182 enzymes: SgfI *
August 16, 2021 14:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2626 3037 3642
Size: 2626 411 605
Fragments arranged by size:
2626 605 411
Acc65I G'GTAC_C
Cuts at: 0 629 1553 3642
Size: 629 924 2089
Fragments arranged by size:
2089 924 629
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2112 3463 3642
Size: 2112 1351 179
Fragments arranged by size:
2112 1351 179
AflIII A'CryG_T
Cuts at: 0 2786 3642
Size: 2786 856
AgeI A'CCGG_T
Cuts at: 0 632 3642
Size: 632 3010
AhdI GACnn_n'nnGTC
Cuts at: 0 1437 3642
Size: 1437 2205
AleI CACnn'nnGTG
Cuts at: 0 544 2034 3642
Size: 544 1490 1608
Fragments arranged by size:
1608 1490 544
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1630 3642
Size: 1630 2012
AlwNI CAG_nnn'CTG
Cuts at: 0 408 2022 2496 2565 2975 3513 3642
Size: 408 1614 474 69 410 538 129
Fragments arranged by size:
1614 538 474 410 408 129 69
ApaI G_GGCC'C
Cuts at: 0 382 2076 2533 2753 3018 3642
Size: 382 1694 457 220 265 624
Fragments arranged by size:
1694 624 457 382 265 220
ApoI r'AATT_y
Cuts at: 0 1114 3202 3642
Size: 1114 2088 440
Fragments arranged by size:
2088 1114 440
AscI GG'CGCG_CC
Cuts at: 0 2330 3642
Size: 2330 1312
AseI AT'TA_AT
Cuts at: 0 2189 3642
Size: 2189 1453
AvaI C'yCGr_G
Cuts at: 0 992 1660 2533 2652 3316 3642
Size: 992 668 873 119 664 326
Fragments arranged by size:
992 873 668 664 326 119
BamHI G'GATC_C
Cuts at: 0 29 291 3642
Size: 29 262 3351
Fragments arranged by size:
3351 262 29
BanI G'GyrC_C
Cuts at: 0 6 113 538 629 1398 1453 1553 1567
Size: 6 107 425 91 769 55 100 14
Cuts at: 1567 2294 2300 2421 3025 3642
Size: 727 6 121 604 617
Fragments arranged by size:
769 727 617 604 425 121 107 100
91 55 14 6 6
BanII G_rGCy'C
Cuts at: 0 382 616 1079 1162 1493 2076 2150 2533
Size: 382 234 463 83 331 583 74 383
Cuts at: 2533 2753 2852 2990 3018 3295 3642
Size: 220 99 138 28 277 347
Fragments arranged by size:
583 463 383 382 347 331 277 234
220 138 99 83 74 28
BbeI G_GCGC'C
Cuts at: 0 117 1402 1571 2298 2425 3642
Size: 117 1285 169 727 127 1217
Fragments arranged by size:
1285 1217 727 169 127 117
BbsI GAAGACnn'nnnn_
Cuts at: 0 58 787 953 2236 2889 3642
Size: 58 729 166 1283 653 753
Fragments arranged by size:
1283 753 729 653 166 58
BbvCI CC'TCA_GC
Cuts at: 0 1170 1345 1519 1761 1817 3642
Size: 1170 175 174 242 56 1825
Fragments arranged by size:
1825 1170 242 175 174 56
BciVI GTATCCnnnnn_n'
Cuts at: 0 2893 3642
Size: 2893 749
BglI GCCn_nnn'nGGC
Cuts at: 0 160 731 877 2258 3642
Size: 160 571 146 1381 1384
Fragments arranged by size:
1384 1381 571 160 146
BglII A'GATC_T
Cuts at: 0 38 2918 3642
Size: 38 2880 724
Fragments arranged by size:
2880 724 38
BlpI GC'TnA_GC
Cuts at: 0 881 3642
Size: 881 2761
Bme1580I G_kGCm'C
Cuts at: 0 342 382 541 1698 1716 2076 2533 2753
Size: 342 40 159 1157 18 360 457 220
Cuts at: 2753 3003 3018 3028 3642
Size: 250 15 10 614
Fragments arranged by size:
1157 614 457 360 342 250 220 159
40 18 15 10
BmgBI CAC'GTC
Cuts at: 0 1506 2787 2952 3452 3642
Size: 1506 1281 165 500 190
Fragments arranged by size:
1506 1281 500 190 165
BmrI ACTGGGnnnn_n'
Cuts at: 0 473 542 3033 3642
Size: 473 69 2491 609
Fragments arranged by size:
2491 609 473 69
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 613 3447 3642
Size: 613 2834 195
Fragments arranged by size:
2834 613 195
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 373 778 1695 1825 1948 2458 3544 3642
Size: 373 405 917 130 123 510 1086 98
Fragments arranged by size:
1086 917 510 405 373 130 123 98
Bpu10I CC'TnA_GC
Cuts at: 0 909 1170 1345 1519 1761 1817 2497 3265
Size: 909 261 175 174 242 56 680 768
Cuts at: 3265 3577 3642
Size: 312 65
Fragments arranged by size:
909 768 680 312 261 242 175 174
65 56
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1051 3642
Size: 1051 2591
BsaI GGTCTCn'nnnn_
Cuts at: 0 931 1114 3642
Size: 931 183 2528
Fragments arranged by size:
2528 931 183
BsaBI GATnn'nnATC
Cuts at: 0 2761 3642
Size: 2761 881
BsaHI Gr'CG_yC
Cuts at: 0 114 1292 1399 1438 1568 1985 2233 2295
Size: 114 1178 107 39 130 417 248 62
Cuts at: 2295 2422 3642
Size: 127 1220
Fragments arranged by size:
1220 1178 417 248 130 127 114 107
62 39
BsaWI w'CCGG_w
Cuts at: 0 581 632 3642
Size: 581 51 3010
Fragments arranged by size:
3010 581 51
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1594 1624 2152 2182 3642
Size: 1594 30 528 30 1460
Fragments arranged by size:
1594 1460 528 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 814 886 1834 1912 1915 2158 2173 3058
Size: 814 72 948 78 3 243 15 885
Cuts at: 3058 3642
Size: 584
Fragments arranged by size:
948 885 814 584 243 78 72 15
3
BseYI C'CCAG_C
Cuts at: 0 363 1464 1530 2220 2238 2745 3296 3483
Size: 363 1101 66 690 18 507 551 187
Cuts at: 3483 3642
Size: 159
Fragments arranged by size:
1101 690 551 507 363 187 159 66
18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 42 457 3297 3642
Size: 42 415 2840 345
Fragments arranged by size:
2840 415 345 42
BsiEI CG_ry'CG
Cuts at: 0 252 489 3642
Size: 252 237 3153
Fragments arranged by size:
3153 252 237
BsiHKAI G_wGCw'C
Cuts at: 0 445 1079 1162 1493 2969 3642
Size: 445 634 83 331 1476 673
Fragments arranged by size:
1476 673 634 445 331 83
BsmBI CGTCTCn'nnnn_
Cuts at: 0 3039 3642
Size: 3039 603
Bsp1286I G_dGCh'C
Cuts at: 0 342 382 445 541 616 1079 1162 1493
Size: 342 40 63 96 75 463 83 331
Cuts at: 1493 1698 1716 2076 2150 2533 2753 2852 2969
Size: 205 18 360 74 383 220 99 117
Cuts at: 2969 2990 3003 3018 3028 3295 3642
Size: 21 13 15 10 267 347
Fragments arranged by size:
463 383 360 347 342 331 267 220
205 117 99 96 83 75 74 63
40 21 18 15 13 10
BspEI T'CCGG_A
Cuts at: 0 581 3642
Size: 581 3061
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 64 416 1691 2378 2626 2921 3037 3632
Size: 64 352 1275 687 248 295 116 595
Cuts at: 3632 3642
Size: 10
Fragments arranged by size:
1275 687 595 352 295 248 116 64
10
BsrBI CCG'CTC
Cuts at: 0 1641 1941 2501 3354 3528 3584 3642
Size: 1641 300 560 853 174 56 58
Fragments arranged by size:
1641 853 560 300 174 58 56
BsrDI GCAATG_nn'
Cuts at: 0 1220 3116 3642
Size: 1220 1896 526
Fragments arranged by size:
1896 1220 526
BsrFI r'CCGG_y
Cuts at: 0 247 488 632 1307 2297 2334 2418 2642
Size: 247 241 144 675 990 37 84 224
Cuts at: 2642 3642
Size: 1000
Fragments arranged by size:
1000 990 675 247 241 224 144 84
37
BssHII G'CGCG_C
Cuts at: 0 1544 2330 3642
Size: 1544 786 1312
Fragments arranged by size:
1544 1312 786
BssSI C'ACGA_G
Cuts at: 0 898 2404 3085 3642
Size: 898 1506 681 557
Fragments arranged by size:
1506 898 681 557
BstAPI GCAn_nnn'nTGC
Cuts at: 0 408 3642
Size: 408 3234
BstEII G'GTnAC_C
Cuts at: 0 171 643 2337 3642
Size: 171 472 1694 1305
Fragments arranged by size:
1694 1305 472 171
BstXI CCAn_nnnn'nTGG
Cuts at: 0 549 561 2349 3054 3642
Size: 549 12 1788 705 588
Fragments arranged by size:
1788 705 588 549 12
BstYI r'GATC_y
Cuts at: 0 29 38 84 291 2918 3642
Size: 29 9 46 207 2627 724
Fragments arranged by size:
2627 724 207 46 29 9
Bsu36I CC'TnA_GG
Cuts at: 0 2854 3642
Size: 2854 788
BtgI C'CryG_G
Cuts at: 0 143 167 200 554 1338 2840 2860 3642
Size: 143 24 33 354 784 1502 20 782
Fragments arranged by size:
1502 784 782 354 143 33 24 20
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 203 3642
Size: 203 3439
BtsI GCAGTG_nn'
Cuts at: 0 143 1905 3547 3642
Size: 143 1762 1642 95
Fragments arranged by size:
1762 1642 143 95
DrdI GACnn_nn'nnGTC
Cuts at: 0 814 1033 3642
Size: 814 219 2609
Fragments arranged by size:
2609 814 219
EaeI y'GGCC_r
Cuts at: 0 140 249 486 524 551 1721 1970 2051
Size: 140 109 237 38 27 1170 249 81
Cuts at: 2051 2541 2837 3089 3566 3642
Size: 490 296 252 477 76
Fragments arranged by size:
1170 490 477 296 252 249 237 140
109 81 76 38 27
EagI C'GGCC_G
Cuts at: 0 249 486 3642
Size: 249 237 3156
Fragments arranged by size:
3156 249 237
EarI CTCTTCn'nnn_
Cuts at: 0 2135 2806 3642
Size: 2135 671 836
Fragments arranged by size:
2135 836 671
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 949 2230 3642
Size: 949 1281 1412
Fragments arranged by size:
1412 1281 949
EcoICRI GAG'CTC
Cuts at: 0 1077 1160 1491 3642
Size: 1077 83 331 2151
Fragments arranged by size:
2151 1077 331 83
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 373 778 1695 1825 1948 2112 2458 3463
Size: 373 405 917 130 123 164 346 1005
Cuts at: 3463 3544 3642
Size: 81 98
Fragments arranged by size:
1005 917 405 373 346 164 130 123
98 81
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1144 1583 1663 3340 3642
Size: 1144 439 80 1677 302
Fragments arranged by size:
1677 1144 439 302 80
EcoO109I rG'GnC_Cy
Cuts at: 0 95 378 379 405 1026 1035 1137 1575
Size: 95 283 1 26 621 9 102 438
Cuts at: 1575 1668 1772 2073 2200 2244 2493 2529 2530
Size: 93 104 301 127 44 249 36 1
Cuts at: 2530 2749 3014 3573 3642
Size: 219 265 559 69
Fragments arranged by size:
621 559 438 301 283 265 249 219
127 104 102 95 93 69 44 36
26 9 1 1
EcoRI G'AATT_C
Cuts at: 0 1114 3202 3642
Size: 1114 2088 440
Fragments arranged by size:
2088 1114 440
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1045 3408 3483 3642
Size: 1045 2363 75 159
Fragments arranged by size:
2363 1045 159 75
FseI GG_CCGG'CC
Cuts at: 0 251 3642
Size: 251 3391
HaeII r_GCGC'y
Cuts at: 0 117 1402 1571 2298 2425 2565 3513 3642
Size: 117 1285 169 727 127 140 948 129
Fragments arranged by size:
1285 948 727 169 140 129 127 117
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 986 1018 1090 1122 1423 1455 1596 1628
Size: 986 32 72 32 301 32 141 32
Cuts at: 1628 3642
Size: 2014
Fragments arranged by size:
2014 986 301 141 72 32 32 32
32
HincII GTy'rAC
Cuts at: 0 75 854 1872 2736 3108 3642
Size: 75 779 1018 864 372 534
Fragments arranged by size:
1018 864 779 534 372 75
HindIII A'AGCT_T
Cuts at: 0 1277 3642
Size: 1277 2365
KasI G'GCGC_C
Cuts at: 0 113 1398 1567 2294 2421 3642
Size: 113 1285 169 727 127 1221
Fragments arranged by size:
1285 1221 727 169 127 113
KpnI G_GTAC'C
Cuts at: 0 633 1557 3642
Size: 633 924 2085
Fragments arranged by size:
2085 924 633
MfeI C'AATT_G
Cuts at: 0 3271 3642
Size: 3271 371
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1430 2963 3642
Size: 1430 1533 679
Fragments arranged by size:
1533 1430 679
MscI TGG'CCA
Cuts at: 0 142 526 553 1723 1972 2053 2543 2839
Size: 142 384 27 1170 249 81 490 296
Cuts at: 2839 3091 3642
Size: 252 551
Fragments arranged by size:
1170 551 490 384 296 252 249 142
81 27
MslI CAynn'nnrTG
Cuts at: 0 324 497 544 559 2034 2347 2950 3121
Size: 324 173 47 15 1475 313 603 171
Cuts at: 3121 3642
Size: 521
Fragments arranged by size:
1475 603 521 324 313 173 171 47
15
MspA1I CmG'CkG
Cuts at: 0 298 1968 2238 2285 2312 2525 2588 2745
Size: 298 1670 270 47 27 213 63 157
Cuts at: 2745 3483 3642
Size: 738 159
Fragments arranged by size:
1670 738 298 270 213 159 157 63
47 27
NaeI GCC'GGC
Cuts at: 0 249 490 2420 2644 3642
Size: 249 241 1930 224 998
Fragments arranged by size:
1930 998 249 241 224
NarI GG'CG_CC
Cuts at: 0 114 1399 1568 2295 2422 3642
Size: 114 1285 169 727 127 1220
Fragments arranged by size:
1285 1220 727 169 127 114
NcoI C'CATG_G
Cuts at: 0 143 554 3642
Size: 143 411 3088
Fragments arranged by size:
3088 411 143
NgoMIV G'CCGG_C
Cuts at: 0 247 488 2418 2642 3642
Size: 247 241 1930 224 1000
Fragments arranged by size:
1930 1000 247 241 224
NspI r_CATG'y
Cuts at: 0 496 1516 1611 3642
Size: 496 1020 95 2031
Fragments arranged by size:
2031 1020 496 95
PasI CC'CwG_GG
Cuts at: 0 569 961 3642
Size: 569 392 2681
Fragments arranged by size:
2681 569 392
PflMI CCAn_nnn'nTGG
Cuts at: 0 522 967 3462 3642
Size: 522 445 2495 180
Fragments arranged by size:
2495 522 445 180
PfoI T'CCnGG_A
Cuts at: 0 87 3197 3642
Size: 87 3110 445
Fragments arranged by size:
3110 445 87
PpuMI rG'GwC_Cy
Cuts at: 0 95 405 1026 1035 1575 2244 3642
Size: 95 310 621 9 540 669 1398
Fragments arranged by size:
1398 669 621 540 310 95 9
PspOMI G'GGCC_C
Cuts at: 0 378 2072 2529 2749 3014 3642
Size: 378 1694 457 220 265 628
Fragments arranged by size:
1694 628 457 378 265 220
PspXI vC'TCGA_Gb
Cuts at: 0 3316 3642
Size: 3316 326
PstI C_TGCA'G
Cuts at: 0 303 404 1043 2918 3642
Size: 303 101 639 1875 724
Fragments arranged by size:
1875 724 639 303 101
PvuII CAG'CTG
Cuts at: 0 298 1968 2588 2745 3483 3642
Size: 298 1670 620 157 738 159
Fragments arranged by size:
1670 738 620 298 159 157
RsrII CG'GwC_CG
Cuts at: 0 122 3642
Size: 122 3520
SacI G_AGCT'C
Cuts at: 0 1079 1162 1493 3642
Size: 1079 83 331 2149
Fragments arranged by size:
2149 1079 331 83
SanDI GG'GwC_CC
Cuts at: 0 1575 2244 3642
Size: 1575 669 1398
Fragments arranged by size:
1575 1398 669
SbfI CC_TGCA'GG
Cuts at: 0 1043 3642
Size: 1043 2599
SexAI A'CCwGG_T
Cuts at: 0 347 3642
Size: 347 3295
SfcI C'TryA_G
Cuts at: 0 299 400 1039 1389 2914 3642
Size: 299 101 639 350 1525 728
Fragments arranged by size:
1525 728 639 350 299 101
SfoI GGC'GCC
Cuts at: 0 115 1400 1569 2296 2423 3642
Size: 115 1285 169 727 127 1219
Fragments arranged by size:
1285 1219 727 169 127 115
SgrAI Cr'CCGG_yG
Cuts at: 0 2297 2334 3642
Size: 2297 37 1308
Fragments arranged by size:
2297 1308 37
SmaI CCC'GGG
Cuts at: 0 2535 2654 3642
Size: 2535 119 988
Fragments arranged by size:
2535 988 119
SmlI C'TyrA_G
Cuts at: 0 1030 3316 3642
Size: 1030 2286 326
Fragments arranged by size:
2286 1030 326
StuI AGG'CCT
Cuts at: 0 1150 1168 3642
Size: 1150 18 2474
Fragments arranged by size:
2474 1150 18
StyI C'CwwG_G
Cuts at: 0 143 542 554 608 3642
Size: 143 399 12 54 3034
Fragments arranged by size:
3034 399 143 54 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 299 547 889 1598 2079 3552 3642
Size: 299 248 342 709 481 1473 90
Fragments arranged by size:
1473 709 481 342 299 248 90
TatI w'GTAC_w
Cuts at: 0 1727 3147 3642
Size: 1727 1420 495
Fragments arranged by size:
1727 1420 495
Tth111I GACn'n_nGTC
Cuts at: 0 658 3642
Size: 658 2984
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2073 3054 3099 3642
Size: 2073 981 45 543
Fragments arranged by size:
2073 981 543 45
XhoI C'TCGA_G
Cuts at: 0 3316 3642
Size: 3316 326
XmaI C'CCGG_G
Cuts at: 0 2533 2652 3642
Size: 2533 119 990
Fragments arranged by size:
2533 990 119
XmnI GAAnn'nnTTC
Cuts at: 0 742 1238 3642
Size: 742 496 2404
Fragments arranged by size:
2404 742 496
Enzymes that do cut:
AarI Acc65I AcuI AflIII AgeI AhdI AleI AlfI
AlwNI ApaI ApoI AscI AseI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FseI HaeII Hin4I
HincII HindIII KasI KpnI MfeI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PflMI
PfoI PpuMI PspOMI PspXI PstI PvuII RsrII SacI
SanDI SbfI SexAI SfcI SfoI SgrAI SmaI SmlI
StuI StyI TaqII TatI Tth111I XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AloI ApaLI AsiSI
AvrII BaeI BcgI BclI BfrBI BmtI BsaAI BsiWI
BsmI BspHI BsrGI BstBI BstZ17I ClaI CspCI DraI
DraIII EcoRV FspI FspAI HpaI MluI NdeI NheI
NotI NruI NsiI PacI PciI PmeI PmlI PpiI
PshAI PsiI PsrI PvuI SacII SalI SapI ScaI
SfiI SgfI SnaBI SpeI SphI SrfI SspI SwaI
TstI XbaI ZraI