##################################
# NO CUT ENZYMES (  158 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AflIII      AgeI        AhdI        
Alw44I      Aor51HI     ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspHI       AspI        AviII       AvrII       BalI        
BanI        BbeI        BbrPI       BbsI        BcgI        
BciVI       BclI        BcnI        BglI        BglII       
BlnI        BpuAI       BsaAI       BsaBI       BsaHI       
BsaWI       BseAI       BsiEI       BsiHKAI     BsiWI       
BsmBI       BsmI        Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BsrDI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstBI       BstEII      
BstPI       BstXI       BstZ17I     Cfr10I      ClaI        
CpoI        DraI        DraIII      DrdI        EagI        
Eam1105I    EarI        EclXI       Eco47III    Eco52I      
EcoO65I     EcoRI       EcoRV       EcoT22I     FbaI        
FseI        FspI        HaeII       Hin1I       HincII      
HindII      HindIII     HpaI        HphI        KasI        
KpnI        Ksp632I     KspI        MaeII       MaeIII      
MamI        MboII       MfeI        MluI        MluNI       
MroI        MscI        MslI        MunI        NaeI        
NarI        NciI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        SwaI        TaqI        Tsp45I      
TspRI       Tth111I     TthHB8I     XbaI        XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   32 enzymes) #
###################################

AfaI         1122  GTAC                 GT^AC
AflII         861  CTTAAG               C^TTAAG
AocI            9  CCTCAGG              CC^T[ACGT]AGG
ApaI          171  GGGCCC               GGGCC^C
AvaII         853  GGTCC                G^G[AT]CC
BamHI        1093  GGATCC               G^GATCC
BbvCI         163  CCTCAGC              CCTCAGC(-5/-2)
BfrI          861  CTTAAG               C^TTAAG
BlpI            3  GCTCAGC              GC^T[ACGT]AGC
BpmI           76  CTCCAG               CTGGAG(16/14)
Bpu1102I        3  GCTCAGC              GC^T[ACGT]AGC
BsaI         1163  GGTCTC               GGTCTC(1/5)
BsgI          243  GTGCAG               GTGCAG(16/14)
BspMI          27  GCAGGT               ACCTGC(4/8)
BsrBI          67  GAGCGG               CCGCTC(-3/-3)
Bsu36I          9  CCTCAGG              CC^T[ACGT]AGG
Cac8I         121  GCCCGC               GC[ACGT]^[ACGT]GC
CelII           3  GCTCAGC              GC^T[ACGT]AGC
DsaI          447  CCGTGG               C^C[GA][CT]GG
EaeI          870  TGGCCG               [CT]^GGCC[GA]
Eco81I          9  CCTCAGG              CC^T[ACGT]AGG
EcoNI         216  CCTCCACCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HapII         873  CCGG                 C^CGG
HgaI           95  GCGTC                GACGC(5/10)
HpaII         873  CCGG                 C^CGG
MseI          862  TTAA                 T^TAA
MspI          873  CCGG                 C^CGG
NlaIII       1110  CATG                 CATG^
PpuMI         852  AGGTCCC              [GA]G^G[AT]CC[CT]
RsaI         1122  GTAC                 GT^AC
SmlI          861  CTTAAG               C^T[CT][GA]AG
Tru9I         862  TTAA                 T^TAA


###################################
# TWO CUT ENZYMES (   10 enzymes) #
###################################

AlwNI         146  CAGCCTCTG            CAG[ACGT][ACGT][ACGT]^CTG
              281  CAGGCCCTG           
AvaI           62  CTCGGG               C^[CT]CG[GA]G
              177  CTCGGG              
BseRI         116  GAGGAG               GAGGAG(10/8)
             1177  GAGGAG              
BsmAI        1102  GTCTC                GTCTC(1/5)
             1164  GTCTC               
BsoBI          62  CTCGGG               C^[CT]CG[GA]G
              177  CTCGGG              
BstAPI         18  GCAACAGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              145  GCAGCCTCTGC         
EcoT14I       843  CCAAGG               C^C[AT][AT]GG
             1085  CCTTGG              
FauI          122  CCCGC                CCCGC(4/6)
              168  GCGGG               
PvuII          22  CAGCTG               CAG^CTG
              266  CAGCTG              
StyI          843  CCAAGG               C^C[AT][AT]GG
             1085  CCTTGG              


#########################################
# 3 OR MORE CUT ENZYMES (   58 enzymes) #
#########################################

AccII       AciI        AluI        AlwI        BanII       
BbvI        BfaI        BmyI        BsaJI       BsiYI       
BslI        BsmFI       Bsp1286I    BsrI        BssKI       
BstF5I      BstNI       BstUI       BstYI       CfoI        
Cfr13I      DdeI        DpnII       DraII       EcoO109I    
EcoRII      Fnu4HI      FokI        HaeIII      HhaI        
HinP1I      HinfI       ItaI        MaeI        MboI        
MflI        MnlI        MspA1I      MvaI        MvnI        
MwoI        NdeII       NlaIV       PflMI       PleI        
PstI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        StuI        TfiI        TseI        Tsp509I     
Van91I      XcmI        XhoII