(Circular) (Six-base) MAPSORT of: pFN21AE0714.seq Check: 1197 from: 1 to: 5938 pFN21AE0714 5938 bp With 182 enzymes: SgfI * December 8, 2014 13:38 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2023 2023 Size: 5938 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3748 278 Size: 53 83 186 936 2212 2468 Fragments arranged by size: 2468 2212 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3281 1937 Size: 1344 4594 Acc65I G'GTAC_C Cuts at: 3259 3259 Size: 5938 AclI AA'CG_TT Cuts at: 1563 4065 4438 1563 Size: 2502 373 3063 Fragments arranged by size: 3063 2502 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4001 5385 5653 1632 Size: 2369 1384 268 1917 Fragments arranged by size: 2369 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 2866 820 Size: 197 1849 3892 Fragments arranged by size: 3892 1849 197 AflIII A'CryG_T Cuts at: 1203 4837 5814 1203 Size: 3634 977 1327 Fragments arranged by size: 3634 1327 977 AgeI A'CCGG_T Cuts at: 4769 4769 Size: 5938 AhdI GACnn_n'nnGTC Cuts at: 4667 4667 Size: 5938 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5938 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5938 AlwNI CAG_nnn'CTG Cuts at: 2156 2291 3681 5253 2156 Size: 135 1390 1572 2841 Fragments arranged by size: 2841 1572 1390 135 ApaI G_GGCC'C Cuts at: 2180 2180 Size: 5938 ApaLI G'TGCA_C Cuts at: 3995 5151 3995 Size: 1156 4782 ApoI r'AATT_y Cuts at: 1493 3247 3473 3625 5734 1493 Size: 1754 226 152 2109 1697 Fragments arranged by size: 2109 1754 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4492 160 Size: 872 3460 1606 Fragments arranged by size: 3460 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5938 AvaI C'yCGr_G Cuts at: 1870 1943 2067 2182 3263 1870 Size: 73 124 115 1081 4545 Fragments arranged by size: 4545 1081 124 115 73 BamHI G'GATC_C Cuts at: 3098 3268 3635 3098 Size: 170 367 5401 Fragments arranged by size: 5401 367 170 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3259 4714 618 Size: 325 217 627 1472 1455 1842 Fragments arranged by size: 1842 1472 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2128 2180 2257 3017 3257 1391 Size: 737 52 77 760 240 4072 Fragments arranged by size: 4072 760 737 240 77 52 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4967 BbvCI CC'TCA_GC Cuts at: 2169 2169 Size: 5938 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4129 4163 1903 Size: 34 50 34 2108 34 3678 Fragments arranged by size: 3678 2108 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3830 5040 3830 Size: 1210 4728 BclI T'GATC_A Cuts at: 1548 1548 Size: 5938 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4549 136 Size: 107 122 71 631 605 2877 1525 Fragments arranged by size: 2877 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5933 5933 Size: 5938 BlpI GC'TnA_GC Cuts at: 2009 3355 2009 Size: 1346 4592 Bme1580I G_kGCm'C Cuts at: 1790 2180 3999 5155 1790 Size: 390 1819 1156 2573 Fragments arranged by size: 2573 1819 1156 390 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5938 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2423 3040 3669 Size: 416 53 83 399 24 1006 617 629 Cuts at: 3669 4627 442 Size: 958 1753 Fragments arranged by size: 1753 1006 958 629 617 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5938 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3118 3208 3118 Size: 90 5848 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2066 4598 1239 Size: 827 2532 2579 Fragments arranged by size: 2579 2532 827 Bpu10I CC'TnA_GC Cuts at: 2169 2932 2944 2169 Size: 763 12 5163 Fragments arranged by size: 5163 763 12 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4063 4928 5226 4063 Size: 865 298 4775 Fragments arranged by size: 4775 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3174 4601 882 Size: 617 1675 1427 2219 Fragments arranged by size: 2219 1675 1427 617 BsaAI yAC'GTr Cuts at: 493 1204 3729 493 Size: 711 2525 2702 Fragments arranged by size: 2702 2525 711 BsaBI GATnn'nnATC Cuts at: 5932 5932 Size: 5938 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3745 4127 4762 275 Size: 53 83 186 936 2212 382 635 1451 Fragments arranged by size: 2212 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4371 4769 5043 5190 5601 4371 Size: 398 274 147 411 4708 Fragments arranged by size: 4708 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5713 Fragments arranged by size: 5713 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2136 3197 1186 Size: 950 1061 3927 Fragments arranged by size: 3927 1061 950 BseYI C'CCAG_C Cuts at: 2094 2269 2320 2374 2398 2482 2813 2818 2956 Size: 175 51 54 24 84 331 5 138 Cuts at: 2956 2986 2992 5141 5637 2094 Size: 30 6 2149 496 2395 Fragments arranged by size: 2395 2149 496 331 175 138 84 54 51 30 24 6 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2269 1163 Size: 573 533 4832 Fragments arranged by size: 4832 573 533 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3388 4149 5177 1540 Size: 275 186 1387 761 1028 2301 Fragments arranged by size: 2301 1387 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3999 4084 5155 3999 Size: 85 1071 4782 Fragments arranged by size: 4782 1071 85 BsmI GAATG_Cn' Cuts at: 3449 3542 3449 Size: 93 5845 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2128 2180 2257 3017 3257 3999 4084 Size: 399 338 52 77 760 240 742 85 Cuts at: 4084 5155 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 760 742 399 338 240 85 77 52 BspEI T'CCGG_A Cuts at: 5601 5601 Size: 5938 BspHI T'CATG_A Cuts at: 3827 5557 3827 Size: 1730 4208 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2023 3293 844 Size: 1179 1270 3489 Fragments arranged by size: 3489 1270 1179 BsrBI CCG'CTC Cuts at: 1394 1823 2074 3825 1394 Size: 429 251 1751 3507 Fragments arranged by size: 3507 1751 429 251 BsrDI GCAATG_nn' Cuts at: 65 1248 4433 4607 65 Size: 1183 3185 174 1396 Fragments arranged by size: 3185 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4582 4769 1700 Size: 110 2772 187 2869 Fragments arranged by size: 2869 2772 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5938 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5938 BssSI C'ACGA_G Cuts at: 3998 5010 3998 Size: 1012 4926 BstAPI GCAn_nnn'nTGC Cuts at: 794 2029 2156 794 Size: 1235 127 4576 Fragments arranged by size: 4576 1235 127 BstBI TT'CG_AA Cuts at: 4742 4742 Size: 5938 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5938 BstYI r'GATC_y Cuts at: 1972 2328 2382 2436 2826 3098 3268 3635 4020 Size: 356 54 54 390 272 170 367 385 Cuts at: 4020 4037 5489 5575 5587 5933 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 390 385 367 356 346 272 170 86 54 54 17 12 Bsu36I CC'TnA_GG Cuts at: 2015 2015 Size: 5938 BtgI C'CryG_G Cuts at: 513 1065 2452 513 Size: 552 1387 3999 Fragments arranged by size: 3999 1387 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5199 Fragments arranged by size: 5199 683 56 BtsI GCAGTG_nn' Cuts at: 3454 4248 4268 3454 Size: 794 20 5124 Fragments arranged by size: 5124 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5938 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5903 DraI TTT'AAA Cuts at: 3242 3242 Size: 5938 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4945 809 Size: 507 3629 1802 Fragments arranged by size: 3629 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2875 3385 4274 Size: 54 1425 50 275 18 1045 510 889 Cuts at: 4274 5903 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1045 889 510 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3385 1537 Size: 275 1573 4090 Fragments arranged by size: 4090 1573 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3868 1353 Size: 2515 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2216 4525 4899 5045 387 Size: 1064 138 627 2309 374 146 1280 Fragments arranged by size: 2309 1280 1064 627 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2066 4001 4598 5385 5653 1239 Size: 393 434 1935 597 787 268 1524 Fragments arranged by size: 1935 1524 787 597 434 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2225 2225 Size: 5938 EcoO109I rG'GnC_Cy Cuts at: 2176 2177 2288 2858 3215 2176 Size: 1 111 570 357 4899 Fragments arranged by size: 4899 570 357 111 1 EcoRI G'AATT_C Cuts at: 3247 3625 3247 Size: 378 5560 FspI TGC'GCA Cuts at: 4444 4444 Size: 5938 HaeII r_GCGC'y Cuts at: 1678 5085 1678 Size: 3407 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3090 3122 4579 4611 4653 Size: 32 135 32 1518 32 1457 32 42 Cuts at: 4653 4685 1373 Size: 32 2626 Fragments arranged by size: 2626 1518 1457 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3282 3528 669 Size: 988 281 1344 246 3079 Fragments arranged by size: 3079 1344 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5938 HpaI GTT'AAC Cuts at: 3528 3528 Size: 5938 KpnI G_GTAC'C Cuts at: 3263 3263 Size: 5938 MfeI C'AATT_G Cuts at: 3537 3537 Size: 5938 MluI A'CGCG_T Cuts at: 5814 5814 Size: 5938 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3051 5052 5236 1305 Size: 1746 2001 184 2007 Fragments arranged by size: 2007 2001 1746 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4116 Fragments arranged by size: 4116 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3896 4255 4414 518 Size: 648 2730 359 159 2042 Fragments arranged by size: 2730 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2029 2173 2198 2273 3354 4031 5179 5424 Size: 423 144 25 75 1081 677 1148 245 Cuts at: 5424 1606 Size: 2120 Fragments arranged by size: 2120 1148 1081 677 423 245 144 75 25 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5386 NdeI CA'TA_TG Cuts at: 387 387 Size: 5938 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5938 NotI GC'GGCC_GC Cuts at: 3385 3385 Size: 5938 NspI r_CATG'y Cuts at: 1132 1229 3296 4841 1132 Size: 97 2067 1545 2229 Fragments arranged by size: 2229 2067 1545 97 PasI CC'CwG_GG Cuts at: 1348 2105 2243 1348 Size: 757 138 5043 Fragments arranged by size: 5043 757 138 PciI A'CATG_T Cuts at: 4837 4837 Size: 5938 PflMI CCAn_nnn'nTGG Cuts at: 1108 2291 2345 2453 1108 Size: 1183 54 108 4593 Fragments arranged by size: 4593 1183 108 54 PmeI GTTT'AAAC Cuts at: 3242 3242 Size: 5938 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4007 4039 5520 5552 4007 Size: 32 1481 32 4393 Fragments arranged by size: 4393 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2858 2858 Size: 5938 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5938 PsiI TTA'TAA Cuts at: 3508 3508 Size: 5938 PspOMI G'GGCC_C Cuts at: 2176 2176 Size: 5938 PstI C_TGCA'G Cuts at: 830 2034 2152 2219 3290 830 Size: 1204 118 67 1071 3478 Fragments arranged by size: 3478 1204 1071 118 67 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5938 PvuII CAG'CTG Cuts at: 2029 2273 2029 Size: 244 5694 SalI G'TCGA_C Cuts at: 1936 3280 1936 Size: 1344 4594 SbfI CC_TGCA'GG Cuts at: 3290 3290 Size: 5938 ScaI AGT'ACT Cuts at: 4186 5898 4186 Size: 1712 4226 SfcI C'TryA_G Cuts at: 826 1045 2030 2148 2215 3286 4421 5102 5293 Size: 219 985 118 67 1071 1135 681 191 Cuts at: 5293 826 Size: 1471 Fragments arranged by size: 1471 1135 1071 985 681 219 191 118 67 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5938 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5938 SmaI CCC'GGG Cuts at: 1872 3265 1872 Size: 1393 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2866 4042 4943 5205 820 Size: 197 926 923 1176 901 262 1553 Fragments arranged by size: 1553 1176 926 923 901 262 197 SnaBI TAC'GTA Cuts at: 493 3729 493 Size: 3236 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5938 SphI G_CATG'C Cuts at: 1132 3296 1132 Size: 2164 3774 SspI AAT'ATT Cuts at: 5 52 3862 5 Size: 47 3810 2081 Fragments arranged by size: 3810 2081 47 StuI AGG'CCT Cuts at: 1517 2231 2751 2907 1517 Size: 714 520 156 4548 Fragments arranged by size: 4548 714 520 156 StyI C'CwwG_G Cuts at: 513 1065 2848 3090 3377 513 Size: 552 1783 242 287 3074 Fragments arranged by size: 3074 1783 552 287 242 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2235 3059 3965 3982 4135 4320 4765 Size: 1255 655 824 906 17 153 185 445 Cuts at: 4765 325 Size: 1498 Fragments arranged by size: 1498 1255 906 824 655 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4184 5896 96 Size: 274 80 33 51 1443 2207 1712 138 Fragments arranged by size: 2207 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5898 5930 5898 Size: 32 5906 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5938 XbaI T'CTAG_A Cuts at: 3274 3274 Size: 5938 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2102 2234 3000 3185 3226 2102 Size: 132 766 185 41 4814 Fragments arranged by size: 4814 766 185 132 41 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5938 XmaI C'CCGG_G Cuts at: 1870 3263 1870 Size: 1393 4545 XmnI GAAnn'nnTTC Cuts at: 4067 4067 Size: 5938 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3746 276 Size: 53 83 186 936 2212 2468 Fragments arranged by size: 2468 2212 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI