################################## # NO CUT ENZYMES ( 143 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcsI AflII Alw44I Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspHI AspI AviII AvrII BamHI BanII BbrPI BbvCI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI BmyI Bpu1102I BsaBI BseAI BsgI BsiHKAI BsiWI BsmBI BsmI Bsp1286I Bsp1407I BspDI BspEI BspHI BspMI BsrBI BsrDI BsrGI BsrI BssHII BssSI Bst1107I BstBI BstUI BstXI BstYI BstZ17I CelII ClaI CpoI DraI DrdI DsaI EarI Eco47III EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KpnI Ksp632I KspI MaeI MamI MfeI MflI MluI MroI MslI MunI MvnI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI SexAI SfaNI SfcI SfiI SfuI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TfiI Tsp509I TspRI Tth111I Van91I XbaI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 67 enzymes) # ################################### AccI 223 GTCTAC GT^[AC][GT]AC AcyI 153 GGCGCC G[GA]^CG[CT]C AfaI 336 GTAC GT^AC AgeI 310 ACCGGT A^CCGGT AhdI 398 GACGCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwI 138 GGATC GGATC(4/5) AlwNI 142 CAGGGGCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 276 CCTGAGG CC^T[ACGT]AGG AspEI 398 GACGCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaI 118 CCCGGG C^[CT]CG[GA]G AvaII 179 GGACC G^G[AT]CC BalI 354 TGGCCA TGG^CCA BanI 153 GGCGCC G^G[CT][GA]CC BbeI 153 GGCGCC GGCGC^C BbsI 243 GAAGAC GAAGAC(2/6) BpmI 88 CTGGAG CTGGAG(16/14) BpuAI 243 GAAGAC GAAGAC(2/6) BsaAI 286 TACGTG [CT]AC^GT[GA] BsaHI 153 GGCGCC G[GA]^CG[CT]C BsaI 91 GAGACC GGTCTC(1/5) BsiEI 392 CGGCCG CG[GA][CT]^CG BsmAI 91 GAGAC GTCTC(1/5) BsoBI 118 CCCGGG C^[CT]CG[GA]G BspLU11I 20 ACATGT A^CATGT BsrFI 310 ACCGGT [GA]^CCGG[CT] BstAPI 188 GCAACTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 313 GGTGACC G^GT[ACGT]ACC BstPI 313 GGTGACC G^GT[ACGT]ACC Bsu36I 276 CCTGAGG CC^T[ACGT]AGG Cfr10I 310 ACCGGT [GA]^CCGG[CT] DraIII 283 CACTACGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 392 CGGCCG C^GGCCG Eam1105I 398 GACGCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 392 CGGCCG C^GGCCG Eco52I 392 CGGCCG C^GGCCG Eco81I 276 CCTGAGG CC^T[ACGT]AGG EcoNI 42 CCTGAGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 313 GGTGACC G^GT[ACGT]ACC HgaI 398 GACGC GACGC(5/10) Hin1I 153 GGCGCC G[GA]^CG[CT]C HinfI 211 GAGTC G^A[ACGT]TC HphI 313 GGTGA GGTGA(8/7) KasI 153 GGCGCC G^GCGCC MaeIII 314 GTGAC ^GT[ACGT]AC MboII 243 GAAGA GAAGA(8/7) MluNI 354 TGGCCA TGG^CCA MscI 354 TGGCCA TGG^CCA MseI 347 TTAA T^TAA NarI 153 GGCGCC GG^CGCC NlaIII 21 CATG CATG^ NspI 20 ACATGT [GA]CATG^[CT] PinAI 310 ACCGGT A^CCGGT PleI 211 GAGTC GAGTC(4/5) PpuMI 178 GGGACCC [GA]G^G[AT]CC[CT] RsaI 336 GTAC GT^AC ScaI 335 AGTACT AGT^ACT SfoI 153 GGCGCC GGC^GCC SgrAI 309 CACCGGTG C[GA]^CCGG[CT]G SmaI 118 CCCGGG CCC^GGG SmlI 331 CTCAAG C^T[CT][GA]AG TaqI 17 TCGA T^CGA Tru9I 347 TTAA T^TAA Tsp45I 314 GTGAC ^GT[GC]AC TthHB8I 17 TCGA T^CGA XcmI 164 CCAACGGGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 118 CCCGGG C^CCGGG XmaI 118 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AflIII 20 ACATGT A^C[GA][CT]GT 287 ACGTGT AluI 5 AGCT AG^CT 239 AGCT BcnI 118 CCCGG CC^[GC]GG 119 CCGGG BsaWI 249 ACCGGA [AT]^CCGG[AT] 310 ACCGGT BseRI 228 CTCCTC GAGGAG(10/8) 106 GAGGAG BstF5I 273 CATCC GGATG(2/0) 252 GGATG DdeI 43 CTGAG C^T[ACGT]AG 277 CTGAG DpnII 66 GATC ^GATC 139 GATC DraII 170 GGGGCCT [GA]G^G[ACGT]CC[CT] 178 GGGACCC EaeI 354 TGGCCA [CT]^GGCC[GA] 392 CGGCCG EcoO109I 170 GGGGCCT [GA]G^G[ACGT]CC[CT] 178 GGGACCC FokI 273 CATCC GGATG(9/13) 252 GGATG HaeII 60 GGCGCT [GA]GCGC^[CT] 153 GGCGCC MaeII 221 ACGT A^CGT 287 ACGT MboI 66 GATC ^GATC 139 GATC NciI 118 CCCGG CC^[GC]GG 119 CCGGG NdeII 66 GATC ^GATC 139 GATC PvuII 4 CAGCTG CAG^CTG 238 CAGCTG Sau3AI 66 GATC ^GATC 139 GATC ######################################### # 3 OR MORE CUT ENZYMES ( 29 enzymes) # ######################################### AciI BbvI BsaJI BsiYI BslI BsmFI BssKI BstNI Cac8I CfoI Cfr13I EcoRII FauI Fnu4HI HaeIII HapII HhaI HinP1I HpaII ItaI MnlI MspA1I MspI MvaI MwoI NlaIV Sau96I ScrFI TseI