(Circular) (Six-base) MAPSORT of: pFN21AE5146.seq Check: 2480 from: 1 to: 5119 pFN21AE5146 5119 bp With 182 enzymes: SgfI * January 30, 2014 17:13 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2929 278 Size: 53 83 186 936 1393 2468 Fragments arranged by size: 2468 1393 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2229 2462 1937 Size: 292 233 4594 Fragments arranged by size: 4594 292 233 Acc65I G'GTAC_C Cuts at: 2440 2440 Size: 5119 AclI AA'CG_TT Cuts at: 1563 3246 3619 1563 Size: 1683 373 3063 Fragments arranged by size: 3063 1683 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2267 3182 4566 4834 1632 Size: 635 915 1384 268 1917 Fragments arranged by size: 1917 1384 915 635 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4922 AflIII A'CryG_T Cuts at: 1203 2025 2292 4018 4995 1203 Size: 822 267 1726 977 1327 Fragments arranged by size: 1726 1327 977 822 267 AgeI A'CCGG_T Cuts at: 2315 3950 2315 Size: 1635 3484 AhdI GACnn_n'nnGTC Cuts at: 2408 3848 2408 Size: 1440 3679 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5119 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5119 AlwNI CAG_nnn'CTG Cuts at: 2152 2862 4434 2152 Size: 710 1572 2837 Fragments arranged by size: 2837 1572 710 ApaLI G'TGCA_C Cuts at: 3176 4332 3176 Size: 1156 3963 ApoI r'AATT_y Cuts at: 1493 2428 2654 2806 4915 1493 Size: 935 226 152 2109 1697 Fragments arranged by size: 2109 1697 935 226 152 AseI AT'TA_AT Cuts at: 160 1032 3673 160 Size: 872 2641 1606 Fragments arranged by size: 2641 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5119 AvaI C'yCGr_G Cuts at: 1870 1943 2123 2444 1870 Size: 73 180 321 4545 Fragments arranged by size: 4545 321 180 73 BamHI G'GATC_C Cuts at: 2449 2816 2449 Size: 367 4752 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2158 2440 3895 618 Size: 325 217 627 371 282 1455 1842 Fragments arranged by size: 1842 1455 627 371 325 282 217 BanII G_rGCy'C Cuts at: 1391 2438 1391 Size: 1047 4072 BbeI G_GCGC'C Cuts at: 2162 2162 Size: 5119 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2255 928 Size: 971 356 3792 Fragments arranged by size: 3792 971 356 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3310 3344 1903 Size: 34 50 34 1289 34 3678 Fragments arranged by size: 3678 1289 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3011 4221 3011 Size: 1210 3909 BclI T'GATC_A Cuts at: 1548 1548 Size: 5119 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3730 136 Size: 107 122 71 631 605 2058 1525 Fragments arranged by size: 2058 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5114 5114 Size: 5119 BlpI GC'TnA_GC Cuts at: 2536 2536 Size: 5119 Bme1580I G_kGCm'C Cuts at: 1790 3180 4336 1790 Size: 1390 1156 2573 Fragments arranged by size: 2573 1390 1156 BmgBI CAC'GTC Cuts at: 1318 2227 1318 Size: 909 4210 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2850 3808 442 Size: 416 53 83 399 24 1433 958 1753 Fragments arranged by size: 1753 1433 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5119 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2114 3779 1239 Size: 875 1665 2579 Fragments arranged by size: 2579 1665 875 Bpu10I CC'TnA_GC Cuts at: 2048 2048 Size: 5119 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2321 3244 4109 4407 2321 Size: 923 865 298 3033 Fragments arranged by size: 3033 923 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2090 3782 882 Size: 617 591 1692 2219 Fragments arranged by size: 2219 1692 617 591 BsaAI yAC'GTr Cuts at: 493 1204 2293 2910 493 Size: 711 1089 617 2702 Fragments arranged by size: 2702 1089 711 617 BsaBI GATnn'nnATC Cuts at: 5113 5113 Size: 5119 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2159 2926 3308 3943 Size: 53 83 186 936 626 767 382 635 Cuts at: 3943 275 Size: 1451 Fragments arranged by size: 1451 936 767 635 626 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2254 2315 3552 3950 4224 4371 4782 2254 Size: 61 1237 398 274 147 411 2591 Fragments arranged by size: 2591 1237 411 398 274 147 61 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2216 2246 1178 Size: 30 165 30 813 30 4051 Fragments arranged by size: 4051 813 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2126 2224 1186 Size: 940 98 4081 Fragments arranged by size: 4081 940 98 BseYI C'CCAG_C Cuts at: 2134 2152 4322 4818 2134 Size: 18 2170 496 2435 Fragments arranged by size: 2435 2170 496 18 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4546 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2400 2569 3330 4358 1540 Size: 275 186 399 169 761 1028 2301 Fragments arranged by size: 2301 1028 761 399 275 186 169 BsiHKAI G_wGCw'C Cuts at: 3180 3265 4336 3180 Size: 85 1071 3963 Fragments arranged by size: 3963 1071 85 BsmI GAATG_Cn' Cuts at: 2630 2723 2630 Size: 93 5026 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2438 3180 3265 4336 1391 Size: 399 648 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 648 399 85 BspEI T'CCGG_A Cuts at: 4782 4782 Size: 5119 BspHI T'CATG_A Cuts at: 3008 4738 3008 Size: 1730 3389 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2474 844 Size: 1630 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3006 1394 Size: 429 1183 3507 Fragments arranged by size: 3507 1183 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3614 3788 65 Size: 1183 2366 174 1396 Fragments arranged by size: 2366 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2315 3763 3950 1700 Size: 110 505 1448 187 2869 Fragments arranged by size: 2869 1448 505 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5119 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5119 BssSI C'ACGA_G Cuts at: 3179 4191 3179 Size: 1012 4107 BstAPI GCAn_nnn'nTGC Cuts at: 794 2199 794 Size: 1405 3714 BstBI TT'CG_AA Cuts at: 3923 3923 Size: 5119 BstEII G'GTnAC_C Cuts at: 1184 2318 1184 Size: 1134 3985 BstYI r'GATC_y Cuts at: 1972 2449 2816 3201 3218 4670 4756 4768 5114 Size: 477 367 385 17 1452 86 12 346 Cuts at: 5114 1972 Size: 1977 Fragments arranged by size: 1977 1452 477 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2282 2282 Size: 5119 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4567 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4380 Fragments arranged by size: 4380 683 56 BtsI GCAGTG_nn' Cuts at: 2635 3429 3449 2635 Size: 794 20 4305 Fragments arranged by size: 4305 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5119 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5084 DraI TTT'AAA Cuts at: 2423 2423 Size: 5119 DraIII CAC_nnn'GTG Cuts at: 2293 2293 Size: 5119 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4126 809 Size: 507 2810 1802 Fragments arranged by size: 2810 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2359 2397 2566 Size: 54 1425 50 275 18 529 38 169 Cuts at: 2566 3455 5084 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 529 275 169 54 50 43 38 18 EagI C'GGCC_G Cuts at: 1537 1812 2397 2566 1537 Size: 275 585 169 4090 Fragments arranged by size: 4090 585 275 169 EarI CTCTTCn'nnn_ Cuts at: 1353 3049 1353 Size: 1696 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3706 4080 4226 387 Size: 1064 138 2117 374 146 1280 Fragments arranged by size: 2117 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2114 2267 3182 3779 4566 4834 1239 Size: 393 482 153 915 597 787 268 1524 Fragments arranged by size: 1524 915 787 597 482 393 268 153 EcoNI CCTnn'n_nnAGG Cuts at: 2051 2051 Size: 5119 EcoO109I rG'GnC_Cy Cuts at: 2176 2184 2176 Size: 8 5111 EcoRI G'AATT_C Cuts at: 2428 2806 2428 Size: 378 4741 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2056 2056 Size: 5119 FspI TGC'GCA Cuts at: 3625 3625 Size: 5119 HaeII r_GCGC'y Cuts at: 1678 2069 2162 4266 1678 Size: 391 93 2104 2531 Fragments arranged by size: 2531 2104 391 93 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2062 2094 2394 2426 3760 Size: 32 135 32 490 32 300 32 1334 Cuts at: 3760 3792 3834 3866 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1334 490 300 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2463 2709 669 Size: 988 281 525 246 3079 Fragments arranged by size: 3079 988 525 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5119 HpaI GTT'AAC Cuts at: 2709 2709 Size: 5119 KasI G'GCGC_C Cuts at: 2158 2158 Size: 5119 KpnI G_GTAC'C Cuts at: 2444 2444 Size: 5119 MfeI C'AATT_G Cuts at: 2718 2718 Size: 5119 MluI A'CGCG_T Cuts at: 4995 4995 Size: 5119 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4233 4417 1305 Size: 2928 184 2007 Fragments arranged by size: 2928 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2361 10 Size: 54 1425 343 529 2768 Fragments arranged by size: 2768 1425 529 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3077 3436 3595 518 Size: 648 1911 359 159 2042 Fragments arranged by size: 2042 1911 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2011 2134 2245 2385 2535 3212 4360 4605 Size: 405 123 111 140 150 677 1148 245 Cuts at: 4605 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 405 245 150 140 123 111 NarI GG'CG_CC Cuts at: 2159 2159 Size: 5119 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4567 NdeI CA'TA_TG Cuts at: 387 387 Size: 5119 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5119 NotI GC'GGCC_GC Cuts at: 2566 2566 Size: 5119 NspI r_CATG'y Cuts at: 1132 1229 2029 2477 4022 1132 Size: 97 800 448 1545 2229 Fragments arranged by size: 2229 1545 800 448 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5119 PciI A'CATG_T Cuts at: 2025 4018 2025 Size: 1993 3126 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5119 PmeI GTTT'AAAC Cuts at: 2423 2423 Size: 5119 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3188 3220 4701 4733 3188 Size: 32 1481 32 3574 Fragments arranged by size: 3574 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2184 2184 Size: 5119 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5119 PsiI TTA'TAA Cuts at: 2689 2689 Size: 5119 PstI C_TGCA'G Cuts at: 830 2471 830 Size: 1641 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5119 PvuII CAG'CTG Cuts at: 2011 2245 2011 Size: 234 4885 SalI G'TCGA_C Cuts at: 1936 2461 1936 Size: 525 4594 SanDI GG'GwC_CC Cuts at: 2184 2184 Size: 5119 SbfI CC_TGCA'GG Cuts at: 2471 2471 Size: 5119 ScaI AGT'ACT Cuts at: 2342 3367 5079 2342 Size: 1025 1712 2382 Fragments arranged by size: 2382 1712 1025 SfcI C'TryA_G Cuts at: 826 1045 2467 3602 4283 4474 826 Size: 219 1422 1135 681 191 1471 Fragments arranged by size: 1471 1422 1135 681 219 191 SfoI GGC'GCC Cuts at: 2160 2160 Size: 5119 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5119 SgrAI Cr'CCGG_yG Cuts at: 1700 2315 1700 Size: 615 4504 SmaI CCC'GGG Cuts at: 1872 2125 2446 1872 Size: 253 321 4545 Fragments arranged by size: 4545 321 253 SmlI C'TyrA_G Cuts at: 820 1017 1943 2336 3223 4124 4386 820 Size: 197 926 393 887 901 262 1553 Fragments arranged by size: 1553 926 901 887 393 262 197 SnaBI TAC'GTA Cuts at: 493 2910 493 Size: 2417 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5119 SphI G_CATG'C Cuts at: 1132 2477 1132 Size: 1345 3774 SspI AAT'ATT Cuts at: 5 52 3043 5 Size: 47 2991 2081 Fragments arranged by size: 2991 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5119 StyI C'CwwG_G Cuts at: 513 1065 2558 513 Size: 552 1493 3074 Fragments arranged by size: 3074 1493 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3146 3163 3316 3501 3946 325 Size: 1255 1566 17 153 185 445 1498 Fragments arranged by size: 1566 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2340 3365 5077 Size: 274 80 33 51 1443 363 1025 1712 Cuts at: 5077 96 Size: 138 Fragments arranged by size: 1712 1443 1025 363 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2216 2248 5079 5111 2216 Size: 32 2831 32 2224 Fragments arranged by size: 2831 2224 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5119 XbaI T'CTAG_A Cuts at: 2455 2455 Size: 5119 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2176 2176 Size: 5119 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5119 XmaI C'CCGG_G Cuts at: 1870 2123 2444 1870 Size: 253 321 4545 Fragments arranged by size: 4545 321 253 XmnI GAAnn'nnTTC Cuts at: 3248 3248 Size: 5119 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2927 276 Size: 53 83 186 936 1393 2468 Fragments arranged by size: 2468 1393 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I EcoICRI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SrfI SwaI