(Circular) (Six-base) MAPSORT of: pFN21AE1860.seq Check: 6052 from: 1 to: 5845 pFN21AE1860 5845 bp With 182 enzymes: SgfI * August 16, 2010 14:50 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2170 2500 2170 Size: 330 5515 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3655 278 Size: 53 83 186 936 2119 2468 Fragments arranged by size: 2468 2119 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3188 1937 Size: 1251 4594 Acc65I G'GTAC_C Cuts at: 3166 3166 Size: 5845 AclI AA'CG_TT Cuts at: 1563 3972 4345 1563 Size: 2409 373 3063 Fragments arranged by size: 3063 2409 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2086 3908 5292 5560 1632 Size: 454 1822 1384 268 1917 Fragments arranged by size: 1917 1822 1384 454 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5648 AflIII A'CryG_T Cuts at: 1203 4744 5721 1203 Size: 3541 977 1327 Fragments arranged by size: 3541 1327 977 AgeI A'CCGG_T Cuts at: 4676 4676 Size: 5845 AhdI GACnn_n'nnGTC Cuts at: 4574 4574 Size: 5845 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5845 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2969 1184 Size: 1785 4060 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2387 2419 3121 3153 2387 Size: 32 702 32 5079 Fragments arranged by size: 5079 702 32 32 AlwNI CAG_nnn'CTG Cuts at: 3124 3588 5160 3124 Size: 464 1572 3809 Fragments arranged by size: 3809 1572 464 ApaI G_GGCC'C Cuts at: 2358 2651 2674 2748 2358 Size: 293 23 74 5455 Fragments arranged by size: 5455 293 74 23 ApaLI G'TGCA_C Cuts at: 3902 5058 3902 Size: 1156 4689 ApoI r'AATT_y Cuts at: 1493 3154 3380 3532 5641 1493 Size: 1661 226 152 2109 1697 Fragments arranged by size: 2109 1697 1661 226 152 AseI AT'TA_AT Cuts at: 160 1032 4399 160 Size: 872 3367 1606 Fragments arranged by size: 3367 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5845 AvaI C'yCGr_G Cuts at: 1870 1943 3170 1870 Size: 73 1227 4545 Fragments arranged by size: 4545 1227 73 BamHI G'GATC_C Cuts at: 3175 3542 3175 Size: 367 5478 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3166 4621 618 Size: 325 217 627 1379 1455 1842 Fragments arranged by size: 1842 1455 1379 627 325 217 BanII G_rGCy'C Cuts at: 1391 2358 2651 2674 2748 3164 1391 Size: 967 293 23 74 416 4072 Fragments arranged by size: 4072 967 416 293 74 23 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2964 928 Size: 971 1065 3809 Fragments arranged by size: 3809 1065 971 BbvCI CC'TCA_GC Cuts at: 3125 3125 Size: 5845 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4036 4070 1903 Size: 34 2099 34 3678 Fragments arranged by size: 3678 2099 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3737 4947 3737 Size: 1210 4635 BclI T'GATC_A Cuts at: 1548 1548 Size: 5845 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2321 2336 2816 Size: 107 122 71 631 605 649 15 480 Cuts at: 2816 4456 136 Size: 1640 1525 Fragments arranged by size: 1640 1525 649 631 605 480 122 107 71 15 BglII A'GATC_T Cuts at: 2115 5840 2115 Size: 3725 2120 BlpI GC'TnA_GC Cuts at: 3262 3262 Size: 5845 Bme1580I G_kGCm'C Cuts at: 1790 2332 2358 2651 2674 2748 3906 5062 1790 Size: 542 26 293 23 74 1158 1156 2573 Fragments arranged by size: 2573 1158 1156 542 293 74 26 23 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5845 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2299 3576 4534 Size: 416 53 83 399 24 882 1277 958 Cuts at: 4534 442 Size: 1753 Fragments arranged by size: 1753 1277 958 882 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5845 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2723 2752 2723 Size: 29 5816 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2526 2718 3001 4505 1239 Size: 1287 192 283 1504 2579 Fragments arranged by size: 2579 1504 1287 283 192 Bpu10I CC'TnA_GC Cuts at: 3125 3125 Size: 5845 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3970 4835 5133 3970 Size: 865 298 4682 Fragments arranged by size: 4682 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2476 2920 2987 4508 882 Size: 617 977 444 67 1521 2219 Fragments arranged by size: 2219 1521 977 617 444 67 BsaAI yAC'GTr Cuts at: 493 1204 3636 493 Size: 711 2432 2702 Fragments arranged by size: 2702 2432 711 BsaBI GATnn'nnATC Cuts at: 5839 5839 Size: 5845 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3652 4034 4669 275 Size: 53 83 186 936 2119 382 635 1451 Fragments arranged by size: 2119 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4278 4676 4950 5097 5508 4278 Size: 398 274 147 411 4615 Fragments arranged by size: 4615 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2387 2417 2616 2646 1178 Size: 30 165 30 984 30 199 30 4377 Fragments arranged by size: 4377 984 199 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2426 2529 2744 2897 1186 Size: 1240 103 215 153 4134 Fragments arranged by size: 4134 1240 215 153 103 BseYI C'CCAG_C Cuts at: 2368 2504 2584 2666 3011 5048 5544 2368 Size: 136 80 82 345 2037 496 2669 Fragments arranged by size: 2669 2037 496 345 136 82 80 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2607 2989 1163 Size: 573 871 382 4019 Fragments arranged by size: 4019 871 573 382 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3295 4056 5084 1540 Size: 275 186 1294 761 1028 2301 Fragments arranged by size: 2301 1294 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2457 3906 3991 5062 2457 Size: 1449 85 1071 3240 Fragments arranged by size: 3240 1449 1071 85 BsmI GAATG_Cn' Cuts at: 3356 3449 3356 Size: 93 5752 BsmBI CGTCTCn'nnnn_ Cuts at: 2006 2006 Size: 5845 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2332 2358 2457 2651 2674 2748 3164 Size: 399 542 26 99 194 23 74 416 Cuts at: 3164 3906 3991 5062 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 542 416 399 194 99 85 74 26 23 BspEI T'CCGG_A Cuts at: 5508 5508 Size: 5845 BspHI T'CATG_A Cuts at: 3734 5464 3734 Size: 1730 4115 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2170 2500 3200 844 Size: 1326 330 700 3489 Fragments arranged by size: 3489 1326 700 330 BsrBI CCG'CTC Cuts at: 1394 1823 3732 1394 Size: 429 1909 3507 Fragments arranged by size: 3507 1909 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4340 4514 65 Size: 1183 3092 174 1396 Fragments arranged by size: 3092 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2315 4489 4676 1700 Size: 110 505 2174 187 2869 Fragments arranged by size: 2869 2174 505 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5845 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5845 BssSI C'ACGA_G Cuts at: 3905 4917 3905 Size: 1012 4833 BstAPI GCAn_nnn'nTGC Cuts at: 794 2693 794 Size: 1899 3946 BstBI TT'CG_AA Cuts at: 4649 4649 Size: 5845 BstEII G'GTnAC_C Cuts at: 1184 2980 1184 Size: 1796 4049 BstYI r'GATC_y Cuts at: 1972 2115 3175 3542 3927 3944 5396 5482 5494 Size: 143 1060 367 385 17 1452 86 12 Cuts at: 5494 5840 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1060 385 367 346 143 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2737 2737 Size: 5845 BtgI C'CryG_G Cuts at: 513 1065 2130 513 Size: 552 1065 4228 Fragments arranged by size: 4228 1065 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2083 487 Size: 683 56 857 4249 Fragments arranged by size: 4249 857 683 56 BtsI GCAGTG_nn' Cuts at: 3361 4155 4175 3361 Size: 794 20 5031 Fragments arranged by size: 5031 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5845 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2824 2859 529 Size: 35 2260 35 3515 Fragments arranged by size: 3515 2260 35 35 DraI TTT'AAA Cuts at: 3149 3149 Size: 5845 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4852 809 Size: 507 3536 1802 Fragments arranged by size: 3536 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2133 2322 2682 Size: 54 1425 50 275 18 303 189 360 Cuts at: 2682 3292 4181 5810 8 Size: 610 889 1629 43 Fragments arranged by size: 1629 1425 889 610 360 303 275 189 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3292 1537 Size: 275 1480 4090 Fragments arranged by size: 4090 1480 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2793 3057 3775 1353 Size: 1440 264 718 3423 Fragments arranged by size: 3423 1440 718 264 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4432 4806 4952 387 Size: 1064 138 2843 374 146 1280 Fragments arranged by size: 2843 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2086 2526 2718 3001 3908 4505 5292 Size: 393 454 440 192 283 907 597 787 Cuts at: 5292 5560 1239 Size: 268 1524 Fragments arranged by size: 1524 907 787 597 454 440 393 283 268 192 EcoNI CCTnn'n_nnAGG Cuts at: 2541 2956 2541 Size: 415 5430 EcoO109I rG'GnC_Cy Cuts at: 2090 2272 2355 2528 2546 2581 2647 2648 2670 Size: 182 83 173 18 35 66 1 22 Cuts at: 2670 2671 2744 2090 Size: 1 73 5191 Fragments arranged by size: 5191 182 173 83 73 66 35 22 18 1 1 EcoRI G'AATT_C Cuts at: 3154 3532 3154 Size: 378 5467 FspI TGC'GCA Cuts at: 4351 4351 Size: 5845 HaeII r_GCGC'y Cuts at: 1678 2467 4992 1678 Size: 789 2525 2531 Fragments arranged by size: 2531 2525 789 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4486 4518 4560 4592 1373 Size: 32 135 32 2914 32 42 32 2626 Fragments arranged by size: 2914 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3189 3435 669 Size: 988 281 1251 246 3079 Fragments arranged by size: 3079 1251 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5845 HpaI GTT'AAC Cuts at: 3435 3435 Size: 5845 KpnI G_GTAC'C Cuts at: 3170 3170 Size: 5845 MfeI C'AATT_G Cuts at: 3444 3444 Size: 5845 MluI A'CGCG_T Cuts at: 5721 5721 Size: 5845 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2322 2430 4959 5143 1305 Size: 1017 108 2529 184 2007 Fragments arranged by size: 2529 2007 1017 184 108 MscI TGG'CCA Cuts at: 10 64 1489 1832 2324 10 Size: 54 1425 343 492 3531 Fragments arranged by size: 3531 1425 492 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2042 3803 4162 4321 518 Size: 648 876 1761 359 159 2042 Fragments arranged by size: 2042 1761 876 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2504 2588 3261 3938 5086 5331 1606 Size: 898 84 673 677 1148 245 2120 Fragments arranged by size: 2120 1148 898 677 673 245 84 NaeI GCC'GGC Cuts at: 2317 2317 Size: 5845 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5293 NdeI CA'TA_TG Cuts at: 387 387 Size: 5845 NgoMIV G'CCGG_C Cuts at: 2315 2315 Size: 5845 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5845 NotI GC'GGCC_GC Cuts at: 3292 3292 Size: 5845 NspI r_CATG'y Cuts at: 1132 1229 3203 4748 1132 Size: 97 1974 1545 2229 Fragments arranged by size: 2229 1974 1545 97 PasI CC'CwG_GG Cuts at: 1348 2931 1348 Size: 1583 4262 PciI A'CATG_T Cuts at: 4744 4744 Size: 5845 PflMI CCAn_nnn'nTGG Cuts at: 1108 2182 1108 Size: 1074 4771 PfoI T'CCnGG_A Cuts at: 3117 3117 Size: 5845 PmeI GTTT'AAAC Cuts at: 3149 3149 Size: 5845 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2387 2419 3914 3946 5427 5459 2387 Size: 32 1495 32 1481 32 2773 Fragments arranged by size: 2773 1495 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2090 2528 2546 2581 2090 Size: 438 18 35 5354 Fragments arranged by size: 5354 438 35 18 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5845 PsiI TTA'TAA Cuts at: 3415 3415 Size: 5845 PspOMI G'GGCC_C Cuts at: 2354 2647 2670 2744 2354 Size: 293 23 74 5455 Fragments arranged by size: 5455 293 74 23 PstI C_TGCA'G Cuts at: 830 2593 3197 830 Size: 1763 604 3478 Fragments arranged by size: 3478 1763 604 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5845 PvuII CAG'CTG Cuts at: 2588 2588 Size: 5845 SalI G'TCGA_C Cuts at: 1936 3187 1936 Size: 1251 4594 SanDI GG'GwC_CC Cuts at: 2090 2581 2090 Size: 491 5354 SbfI CC_TGCA'GG Cuts at: 3197 3197 Size: 5845 ScaI AGT'ACT Cuts at: 4093 5805 4093 Size: 1712 4133 SexAI A'CCwGG_T Cuts at: 2381 2381 Size: 5845 SfcI C'TryA_G Cuts at: 826 1045 2589 3035 3193 4328 5009 5200 826 Size: 219 1544 446 158 1135 681 191 1471 Fragments arranged by size: 1544 1471 1135 681 446 219 191 158 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5845 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5845 SmaI CCC'GGG Cuts at: 1872 3172 1872 Size: 1300 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3949 4850 5112 820 Size: 197 926 2006 901 262 1553 Fragments arranged by size: 2006 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3636 493 Size: 3143 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5845 SphI G_CATG'C Cuts at: 1132 3203 1132 Size: 2071 3774 SspI AAT'ATT Cuts at: 5 52 3769 5 Size: 47 3717 2081 Fragments arranged by size: 3717 2081 47 StuI AGG'CCT Cuts at: 1517 2099 2679 2735 1517 Size: 582 580 56 4627 Fragments arranged by size: 4627 582 580 56 StyI C'CwwG_G Cuts at: 513 1065 2859 2957 3284 513 Size: 552 1794 98 327 3074 Fragments arranged by size: 3074 1794 552 327 98 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2377 3872 3889 4042 4227 4672 325 Size: 1255 797 1495 17 153 185 445 1498 Fragments arranged by size: 1498 1495 1255 797 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4091 5803 96 Size: 274 80 33 51 1443 2114 1712 138 Fragments arranged by size: 2114 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2616 2648 5805 5837 2616 Size: 32 3157 32 2624 Fragments arranged by size: 3157 2624 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5845 XbaI T'CTAG_A Cuts at: 3181 3181 Size: 5845 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5845 XmaI C'CCGG_G Cuts at: 1870 3170 1870 Size: 1300 4545 XmnI GAAnn'nnTTC Cuts at: 3974 3974 Size: 5845 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3653 276 Size: 53 83 186 936 2119 2468 Fragments arranged by size: 2468 2119 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbeI BfrBI BsiWI BstXI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SapI SfiI SfoI SrfI SwaI XcmI