Restriction map for
pFN21AB9439
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# NO CUT ENZYMES ( 112 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AviII AvrII BamHI BbrPI BcgI
BciVI BclI BfrI BglII BlnI
BsaAI BsaBI BsaI BsaWI BseAI
BsiWI BsmI Bsp1407I BspDI BspEI
BspHI BsrBI BsrDI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstZ17I Bsu36I ClaI CpoI DraI
DraIII DrdI Eam1105I Eco47III Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HpaI KpnI
KspI MamI MfeI MluI MroI
MseI MslI MunI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SbfI ScaI SexAI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI Tru9I XbaI XcmI
XhoI XmnI
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# ONE CUT ENZYMES ( 58 enzymes) #
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AcyI 995 GGCGCC G[GA]^CG[CT]C
AfaI 179 GTAC GT^AC
AflIII 710 ACATGT A^C[GA][CT]GT
ApaI 1350 GGGCCC GGGCC^C
AspI 759 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 629 TGGCCA TGG^CCA
BanI 995 GGCGCC G^G[CT][GA]CC
BbeI 995 GGCGCC GGCGC^C
BbsI 196 GAAGAC GAAGAC(2/6)
BlpI 798 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 798 GCTGAGC GC^T[ACGT]AGC
BpuAI 196 GAAGAC GAAGAC(2/6)
BsaHI 995 GGCGCC G[GA]^CG[CT]C
BsgI 952 CTGCAC GTGCAG(16/14)
BsiEI 834 CGGCCG CG[GA][CT]^CG
BsmBI 840 GAGACG CGTCTC(1/5)
BspLU11I 710 ACATGT A^CATGT
BspMI 366 ACCTGC ACCTGC(4/8)
BstAPI 330 GCAGCTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 929 AGATCC [GA]^GATC[CT]
CelII 798 GCTGAGC GC^T[ACGT]AGC
DsaI 632 CCATGG C^C[GA][CT]GG
EagI 834 CGGCCG C^GGCCG
EclXI 834 CGGCCG C^GGCCG
Eco52I 834 CGGCCG C^GGCCG
HaeII 995 GGCGCC [GA]GCGC^[CT]
Hin1I 995 GGCGCC G[GA]^CG[CT]C
HincII 279 GTTGAC GT[CT]^[GA]AC
HindII 279 GTTGAC GT[CT]^[GA]AC
HindIII 1124 AAGCTT A^AGCTT
HphI 616 GGTGA GGTGA(8/7)
KasI 995 GGCGCC G^GCGCC
MaeII 872 ACGT A^CGT
MflI 929 AGATCC [GA]^GATC[CT]
MluNI 629 TGGCCA TGG^CCA
MscI 629 TGGCCA TGG^CCA
NarI 995 GGCGCC GG^CGCC
NcoI 632 CCATGG C^CATGG
NdeI 1078 CATATG CA^TATG
NheI 294 GCTAGC G^CTAGC
NspI 710 ACATGT [GA]CATG^[CT]
PflFI 759 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 327 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1105 GGGACCT [GA]G^G[AT]CC[CT]
PshAI 867 GACAAACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 1023 CTGCAG CTGCA^G
RsaI 179 GTAC GT^AC
SacI 458 GAGCTC GAGCT^C
SapI 460 GCTCTTC GCTCTTC(1/4)
SfiI 826 GGCCTGCCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 995 GGCGCC GGC^GCC
SmaI 479 CCCGGG CCC^GGG
TfiI 211 GATTC G^A[AT]TC
Tth111I 759 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 327 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 929 AGATCC [GA]^GATC[CT]
XmaCI 479 CCCGGG C^CCGGG
XmaI 479 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccII 976 CGCG CG^CG
1101 CGCG
AlwI 930 GATCC GGATC(4/5)
972 GATCC
AlwNI 328 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
373 CAGTTCCTG
AvaI 479 CCCGGG C^[CT]CG[GA]G
532 CCCGAG
BfaI 295 CTAG C^TAG
901 CTAG
BsoBI 479 CCCGGG C^[CT]CG[GA]G
532 CCCGAG
BstF5I 524 CATCC GGATG(2/0)
1245 CATCC
BstUI 976 CGCG CG^CG
1101 CGCG
EcoT14I 632 CCATGG C^C[AT][AT]GG
1092 CCAAGG
FokI 524 CATCC GGATG(9/13)
1245 CATCC
MaeI 295 CTAG C^TAG
901 CTAG
MvnI 976 CGCG CG^CG
1101 CGCG
NaeI 243 GCCGGC GCC^GGC
1059 GCCGGC
NgoMIV 243 GCCGGC G^CCGGC
1059 GCCGGC
PleI 73 GACTC GAGTC(4/5)
772 GACTC
SfcI 1023 CTGCAG C^T[GA][CT]AG
1068 CTACAG
StuI 408 AGGCCT AGG^CCT
1332 AGGCCT
StyI 632 CCATGG C^C[AT][AT]GG
1092 CCAAGG
Tsp45I 1084 GTCAC ^GT[GC]AC
1255 GTCAC
Tsp509I 566 AATT ^AATT
1240 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AciI AluI AspHI AvaII BanII
BbvCI BbvI BcnI BglI BmyI
BpmI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrFI
BsrI BssKI BstNI BstXI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII EaeI EarI EcoO109I EcoRII
FauI Fnu4HI HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII ItaI
Ksp632I MaeIII MboI MboII MnlI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PvuII Sau3AI
Sau96I ScrFI SfaNI SmlI TaqI
TseI TspRI TthHB8I