(Circular) (Six-base) MAPSORT of: pFN21AB9439.seq Check: 2433 from: 1 to: 6094 pFN21AB9439 6094 bp With 182 enzymes: SgfI * August 16, 2010 14:44 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2380 2380 Size: 6094 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3904 278 Size: 53 83 186 936 2368 2468 Fragments arranged by size: 2468 2368 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3437 1937 Size: 1500 4594 Acc65I G'GTAC_C Cuts at: 3415 3415 Size: 6094 AclI AA'CG_TT Cuts at: 1563 4221 4594 1563 Size: 2658 373 3063 Fragments arranged by size: 3063 2658 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2220 2647 4157 5541 5809 1632 Size: 588 427 1510 1384 268 1917 Fragments arranged by size: 1917 1510 1384 588 427 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5897 AflIII A'CryG_T Cuts at: 1203 2715 4993 5970 1203 Size: 1512 2278 977 1327 Fragments arranged by size: 2278 1512 1327 977 AgeI A'CCGG_T Cuts at: 4925 4925 Size: 6094 AhdI GACnn_n'nnGTC Cuts at: 4823 4823 Size: 6094 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6094 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6094 AlwNI CAG_nnn'CTG Cuts at: 2338 2383 3837 5409 2338 Size: 45 1454 1572 3023 Fragments arranged by size: 3023 1572 1454 45 ApaI G_GGCC'C Cuts at: 3359 3359 Size: 6094 ApaLI G'TGCA_C Cuts at: 4151 5307 4151 Size: 1156 4938 ApoI r'AATT_y Cuts at: 1493 3403 3629 3781 5890 1493 Size: 1910 226 152 2109 1697 Fragments arranged by size: 2109 1910 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4648 160 Size: 872 3616 1606 Fragments arranged by size: 3616 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6094 AvaI C'yCGr_G Cuts at: 1870 1943 2484 2537 3419 1870 Size: 73 541 53 882 4545 Fragments arranged by size: 4545 882 541 73 53 BamHI G'GATC_C Cuts at: 3424 3791 3424 Size: 367 5727 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3000 3415 4870 618 Size: 325 217 627 1213 415 1455 1842 Fragments arranged by size: 1842 1455 1213 627 415 325 217 BanII G_rGCy'C Cuts at: 1391 2029 2467 2547 3359 3413 1391 Size: 638 438 80 812 54 4072 Fragments arranged by size: 4072 812 638 438 80 54 BbeI G_GCGC'C Cuts at: 3004 3004 Size: 6094 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2208 928 Size: 971 309 4814 Fragments arranged by size: 4814 971 309 BbvCI CC'TCA_GC Cuts at: 2244 2448 3169 2244 Size: 204 721 5169 Fragments arranged by size: 5169 721 204 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4285 4319 1903 Size: 34 2348 34 3678 Fragments arranged by size: 3678 2348 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3986 5196 3986 Size: 1210 4884 BclI T'GATC_A Cuts at: 1548 1548 Size: 6094 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2412 2838 3309 Size: 107 122 71 631 605 740 426 471 Cuts at: 3309 4705 136 Size: 1396 1525 Fragments arranged by size: 1525 1396 740 631 605 471 426 122 107 71 BglII A'GATC_T Cuts at: 6089 6089 Size: 6094 BlpI GC'TnA_GC Cuts at: 2804 3511 2804 Size: 707 5387 Bme1580I G_kGCm'C Cuts at: 1790 3359 4155 5311 1790 Size: 1569 796 1156 2573 Fragments arranged by size: 2573 1569 1156 796 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6094 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2175 2202 2541 Size: 416 53 83 399 24 758 27 339 Cuts at: 2541 3825 4783 442 Size: 1284 958 1753 Fragments arranged by size: 1753 1284 958 758 416 399 339 83 53 27 24 BmtI G_CTAG'C Cuts at: 1056 2303 1056 Size: 1247 4847 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1999 2013 2075 2309 2315 2348 2576 2585 Size: 760 14 62 234 6 33 228 9 Cuts at: 2585 2759 3225 4754 1239 Size: 174 466 1529 2579 Fragments arranged by size: 2579 1529 760 466 234 228 174 62 33 14 9 6 Bpu10I CC'TnA_GC Cuts at: 2244 2448 2702 3169 2244 Size: 204 254 467 5169 Fragments arranged by size: 5169 467 254 204 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2711 2764 3291 4219 5084 5382 2711 Size: 53 527 928 865 298 3423 Fragments arranged by size: 3423 928 865 527 298 53 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4757 882 Size: 617 3258 2219 Fragments arranged by size: 3258 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3885 493 Size: 711 2681 2702 Fragments arranged by size: 2702 2681 711 BsaBI GATnn'nnATC Cuts at: 6088 6088 Size: 6094 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3001 3901 4283 4918 Size: 53 83 186 936 1468 900 382 635 Cuts at: 4918 275 Size: 1451 Fragments arranged by size: 1468 1451 936 900 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4527 4925 5199 5346 5757 4527 Size: 398 274 147 411 4864 Fragments arranged by size: 4864 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2304 2334 1178 Size: 30 165 30 901 30 4938 Fragments arranged by size: 4938 901 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2312 2555 2573 2945 1186 Size: 1126 243 18 372 4335 Fragments arranged by size: 4335 1126 372 243 18 BseYI C'CCAG_C Cuts at: 2224 2253 2303 2397 2478 2605 2789 2813 3050 Size: 29 50 94 81 127 184 24 237 Cuts at: 3050 3191 3299 3347 5297 5793 2224 Size: 141 108 48 1950 496 2525 Fragments arranged by size: 2525 1950 496 237 184 141 127 108 94 81 50 48 29 24 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2942 1163 Size: 573 1206 4315 Fragments arranged by size: 4315 1206 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2842 3544 4305 5333 1540 Size: 275 186 841 702 761 1028 2301 Fragments arranged by size: 2301 1028 841 761 702 275 186 BsiHKAI G_wGCw'C Cuts at: 2094 2467 3149 4155 4240 5311 2094 Size: 373 682 1006 85 1071 2877 Fragments arranged by size: 2877 1071 1006 682 373 85 BsmI GAATG_Cn' Cuts at: 3605 3698 3605 Size: 93 6001 BsmBI CGTCTCn'nnnn_ Cuts at: 2839 2839 Size: 6094 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2029 2094 2467 2547 3149 3359 3413 Size: 399 239 65 373 80 602 210 54 Cuts at: 3413 4155 4240 5311 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 602 399 373 239 210 85 80 65 54 BspEI T'CCGG_A Cuts at: 5757 5757 Size: 6094 BspHI T'CATG_A Cuts at: 3983 5713 3983 Size: 1730 4364 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2380 3449 844 Size: 1536 1069 3489 Fragments arranged by size: 3489 1536 1069 BsrBI CCG'CTC Cuts at: 1394 1823 3981 1394 Size: 429 2158 3507 Fragments arranged by size: 3507 2158 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4589 4763 65 Size: 1183 3341 174 1396 Fragments arranged by size: 3341 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2248 2997 3064 4738 4925 1700 Size: 110 438 749 67 1674 187 2869 Fragments arranged by size: 2869 1674 749 438 187 110 67 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6094 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6094 BssSI C'ACGA_G Cuts at: 4154 5166 4154 Size: 1012 5082 BstAPI GCAn_nnn'nTGC Cuts at: 794 2341 794 Size: 1547 4547 BstBI TT'CG_AA Cuts at: 4898 4898 Size: 6094 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6094 BstXI CCAn_nnnn'nTGG Cuts at: 2303 2518 2554 2303 Size: 215 36 5843 Fragments arranged by size: 5843 215 36 BstYI r'GATC_y Cuts at: 1972 2934 3424 3791 4176 4193 5645 5731 5743 Size: 962 490 367 385 17 1452 86 12 Cuts at: 5743 6089 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 962 490 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2637 513 Size: 552 939 633 3970 Fragments arranged by size: 3970 939 633 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5355 Fragments arranged by size: 5355 683 56 BtsI GCAGTG_nn' Cuts at: 3037 3610 4404 4424 3037 Size: 573 794 20 4707 Fragments arranged by size: 4707 794 573 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6094 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6059 DraI TTT'AAA Cuts at: 3398 3398 Size: 6094 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5101 809 Size: 507 3785 1802 Fragments arranged by size: 3785 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2634 2839 3066 Size: 54 1425 50 275 18 804 205 227 Cuts at: 3066 3276 3541 4430 6059 8 Size: 210 265 889 1629 43 Fragments arranged by size: 1629 1425 889 804 275 265 227 210 205 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2839 3541 1537 Size: 275 1027 702 4090 Fragments arranged by size: 4090 1027 702 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2472 2849 2891 4024 1353 Size: 1119 377 42 1133 3423 Fragments arranged by size: 3423 1133 1119 377 42 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4681 5055 5201 387 Size: 1064 138 3092 374 146 1280 Fragments arranged by size: 3092 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2465 2465 Size: 6094 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 1999 2013 2075 2220 2309 2315 2348 Size: 393 367 14 62 145 89 6 33 Cuts at: 2348 2576 2585 2647 2759 3225 4157 4754 5541 Size: 228 9 62 112 466 932 597 787 Cuts at: 5541 5809 1239 Size: 268 1524 Fragments arranged by size: 1524 932 787 597 466 393 367 268 228 145 112 89 62 62 33 14 9 6 EcoO109I rG'GnC_Cy Cuts at: 2692 3111 3356 2692 Size: 419 245 5430 Fragments arranged by size: 5430 419 245 EcoRI G'AATT_C Cuts at: 3403 3781 3403 Size: 378 5716 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2626 2626 Size: 6094 FspI TGC'GCA Cuts at: 4600 4600 Size: 6094 HaeII r_GCGC'y Cuts at: 1678 3004 5241 1678 Size: 1326 2237 2531 Fragments arranged by size: 2531 2237 1326 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2069 2101 2416 2448 2513 Size: 32 135 32 497 32 315 32 65 Cuts at: 2513 2545 2753 2785 4735 4767 4809 4841 1373 Size: 32 208 32 1950 32 42 32 2626 Fragments arranged by size: 2626 1950 497 315 208 135 65 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2286 3438 3684 669 Size: 988 281 348 1152 246 3079 Fragments arranged by size: 3079 1152 988 348 281 246 HindIII A'AGCT_T Cuts at: 748 3129 748 Size: 2381 3713 HpaI GTT'AAC Cuts at: 3684 3684 Size: 6094 KasI G'GCGC_C Cuts at: 3000 3000 Size: 6094 KpnI G_GTAC'C Cuts at: 3419 3419 Size: 6094 MfeI C'AATT_G Cuts at: 3693 3693 Size: 6094 MluI A'CGCG_T Cuts at: 5970 5970 Size: 6094 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5208 5392 1305 Size: 3903 184 2007 Fragments arranged by size: 3903 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2636 10 Size: 54 1425 343 804 3468 Fragments arranged by size: 3468 1425 804 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4052 4411 4570 518 Size: 648 2886 359 159 2042 Fragments arranged by size: 2886 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2338 2803 2956 3173 3203 3510 4187 5335 Size: 732 465 153 217 30 307 677 1148 Cuts at: 5335 5580 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 732 677 465 307 245 217 153 30 NaeI GCC'GGC Cuts at: 2250 3066 2250 Size: 816 5278 NarI GG'CG_CC Cuts at: 3001 3001 Size: 6094 NcoI C'CATG_G Cuts at: 513 1065 2004 2637 513 Size: 552 939 633 3970 Fragments arranged by size: 3970 939 633 552 NdeI CA'TA_TG Cuts at: 387 3084 387 Size: 2697 3397 NgoMIV G'CCGG_C Cuts at: 2248 3064 2248 Size: 816 5278 NheI G'CTAG_C Cuts at: 1052 2299 1052 Size: 1247 4847 NotI GC'GGCC_GC Cuts at: 3541 3541 Size: 6094 NspI r_CATG'y Cuts at: 1132 1229 2719 3452 4997 1132 Size: 97 1490 733 1545 2229 Fragments arranged by size: 2229 1545 1490 733 97 PasI CC'CwG_GG Cuts at: 1348 2458 3056 1348 Size: 1110 598 4386 Fragments arranged by size: 4386 1110 598 PciI A'CATG_T Cuts at: 2715 4993 2715 Size: 2278 3816 PflMI CCAn_nnn'nTGG Cuts at: 1108 2338 1108 Size: 1230 4864 PfoI T'CCnGG_A Cuts at: 2736 2736 Size: 6094 PmeI GTTT'AAAC Cuts at: 3398 3398 Size: 6094 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4163 4195 5676 5708 4163 Size: 32 1481 32 4549 Fragments arranged by size: 4549 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3111 3111 Size: 6094 PshAI GACnn'nnGTC Cuts at: 1613 2876 1613 Size: 1263 4831 PsiI TTA'TAA Cuts at: 3664 3664 Size: 6094 PspOMI G'GGCC_C Cuts at: 3355 3355 Size: 6094 PstI C_TGCA'G Cuts at: 830 3032 3446 830 Size: 2202 414 3478 Fragments arranged by size: 3478 2202 414 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6094 PvuII CAG'CTG Cuts at: 2338 2803 2956 3173 3203 2338 Size: 465 153 217 30 5229 Fragments arranged by size: 5229 465 217 153 30 SacI G_AGCT'C Cuts at: 2467 2467 Size: 6094 SalI G'TCGA_C Cuts at: 1936 3436 1936 Size: 1500 4594 SapI GCTCTTCn'nnn_ Cuts at: 2472 2472 Size: 6094 SbfI CC_TGCA'GG Cuts at: 3446 3446 Size: 6094 ScaI AGT'ACT Cuts at: 4342 6054 4342 Size: 1712 4382 SfcI C'TryA_G Cuts at: 826 1045 3028 3073 3442 4577 5258 5449 826 Size: 219 1983 45 369 1135 681 191 1471 Fragments arranged by size: 1983 1471 1135 681 369 219 191 45 SfiI GGCCn_nnn'nGGCC Cuts at: 2838 2838 Size: 6094 SfoI GGC'GCC Cuts at: 3002 3002 Size: 6094 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6094 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6094 SmaI CCC'GGG Cuts at: 1872 2486 3421 1872 Size: 614 935 4545 Fragments arranged by size: 4545 935 614 SmlI C'TyrA_G Cuts at: 820 1017 1943 2726 2779 3306 4198 5099 5361 Size: 197 926 783 53 527 892 901 262 Cuts at: 5361 820 Size: 1553 Fragments arranged by size: 1553 926 901 892 783 527 262 197 53 SnaBI TAC'GTA Cuts at: 493 3885 493 Size: 3392 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6094 SphI G_CATG'C Cuts at: 1132 3452 1132 Size: 2320 3774 SspI AAT'ATT Cuts at: 5 52 4018 5 Size: 47 3966 2081 Fragments arranged by size: 3966 2081 47 StuI AGG'CCT Cuts at: 1517 2415 3339 1517 Size: 898 924 4272 Fragments arranged by size: 4272 924 898 StyI C'CwwG_G Cuts at: 513 1065 2004 2637 3097 3533 513 Size: 552 939 633 460 436 3074 Fragments arranged by size: 3074 939 633 552 460 436 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4121 4138 4291 4476 4921 325 Size: 1255 2541 17 153 185 445 1498 Fragments arranged by size: 2541 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2183 4340 6052 Size: 274 80 33 51 1443 206 2157 1712 Cuts at: 6052 96 Size: 138 Fragments arranged by size: 2157 1712 1443 274 206 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6054 6086 6054 Size: 32 6062 Tth111I GACn'n_nGTC Cuts at: 1316 2767 1316 Size: 1451 4643 XbaI T'CTAG_A Cuts at: 3430 3430 Size: 6094 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6094 XmaI C'CCGG_G Cuts at: 1870 2484 3419 1870 Size: 614 935 4545 Fragments arranged by size: 4545 935 614 XmnI GAAnn'nnTTC Cuts at: 4223 4223 Size: 6094 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3902 276 Size: 53 83 186 936 2368 2468 Fragments arranged by size: 2468 2368 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SanDI SexAI SrfI SwaI XcmI