################################## # NO CUT ENZYMES ( 211 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI AlwI AlwNI AocI Aor51HI ApaI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BsaBI BsaHI BsaI BsaJI BsaWI BseAI BsgI BsiEI BsiWI BsiYI BslI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrDI BsrFI BsrGI BsrI BssHII BssKI BssSI Bst1107I BstAPI BstBI BstEII BstNI BstPI BstUI BstXI BstYI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I ClaI CpoI DpnII DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRII EcoT14I EcoT22I FbaI FseI FspI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII HphI KasI KpnI Ksp632I KspI MaeI MaeIII MamI MboI MfeI MflI MluI MluNI MroI MscI MseI MspI MunI MvaI MvnI NaeI NarI NciI NcoI NdeI NdeII NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI SapI Sau3AI Sau96I SbfI ScaI ScrFI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI Tru9I Tsp45I Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 31 enzymes) # ################################### AciI 349 CCGC CCGC(-3/-1) AfaI 283 GTAC GT^AC Alw44I 166 GTGCAC G^TGCAC ApaLI 166 GTGCAC G^TGCAC AspHI 166 GTGCAC G[AT]GC[AT]^C BbsI 3 GTCTTC GAAGAC(2/6) BbvI 105 GCTGC GCAGC(8/12) BpuAI 3 GTCTTC GAAGAC(2/6) BseRI 223 GAGGAG GAGGAG(10/8) BsiHKAI 166 GTGCAC G[AT]GC[AT]^C BsmAI 226 GAGAC GTCTC(1/5) BsmFI 70 GGGAC GGGAC(10/14) BspHI 188 TCATGA T^CATGA BspMI 108 GCAGGT ACCTGC(4/8) DdeI 399 CTCAG C^T[ACGT]AG EcoRV 385 GATATC GAT^ATC FauI 348 CCCGC CCCGC(4/6) Fnu4HI 105 GCTGC GC^[ACGT]GC ItaI 105 GCTGC GC^[ACGT]GC MspA1I 103 CAGCTG C[AC]G^C[GT]G MwoI 323 GCATTTACGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NlaIV 49 GGTTCC GG[ACGT]^[ACGT]CC PstI 106 CTGCAG CTGCA^G PvuII 103 CAGCTG CAG^CTG RcaI 188 TCATGA T^CATGA RsaI 283 GTAC GT^AC SfaNI 275 GCATC GCATC(5/9) SfcI 106 CTGCAG C^T[GA][CT]AG SnaBI 280 TACGTA TAC^GTA TseI 105 GCTGC G^C[AT]GC Tsp509I 263 AATT ^AATT ################################### # TWO CUT ENZYMES ( 12 enzymes) # ################################### AluI 104 AGCT AG^CT 269 AGCT BmyI 166 GTGCAC G[AGT]GC[ACT]^C 241 GTGCCC BsaAI 238 TACGTG [CT]AC^GT[GA] 280 TACGTA Bsp1286I 166 GTGCAC G[AGT]GC[ACT]^C 241 GTGCCC HinfI 139 GATTC G^A[ACGT]TC 344 GAATC MaeII 239 ACGT A^CGT 281 ACGT MboII 363 GAAGA GAAGA(8/7) 4 TCTTC MslI 123 CACTGACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 286 CATTACAATG TaqI 342 TCGA T^CGA 379 TCGA TfiI 139 GATTC G^A[AT]TC 344 GAATC TspRI 123 CACTG CA[GC]TG[ACGT][ACGT]^ 401 CAGTG TthHB8I 342 TCGA T^CGA 379 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 4 enzymes) # ######################################### BstF5I FokI MnlI NlaIII