(Circular) (Six-base) MAPSORT of: pFN21AE6513.seq Check: 627 from: 1 to: 5122 pFN21AE6513 5122 bp With 182 enzymes: SgfI * March 18, 2015 14:52 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2104 2104 Size: 5122 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2932 278 Size: 53 83 186 936 1396 2468 Fragments arranged by size: 2468 1396 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2465 1937 Size: 528 4594 Acc65I G'GTAC_C Cuts at: 2443 2443 Size: 5122 AclI AA'CG_TT Cuts at: 1563 3249 3622 1563 Size: 1686 373 3063 Fragments arranged by size: 3063 1686 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3185 4569 4837 1632 Size: 1553 1384 268 1917 Fragments arranged by size: 1917 1553 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4925 AflIII A'CryG_T Cuts at: 1203 4021 4998 1203 Size: 2818 977 1327 Fragments arranged by size: 2818 1327 977 AgeI A'CCGG_T Cuts at: 3953 3953 Size: 5122 AhdI GACnn_n'nnGTC Cuts at: 3851 3851 Size: 5122 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5122 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5122 AlwNI CAG_nnn'CTG Cuts at: 2865 4437 2865 Size: 1572 3550 ApaLI G'TGCA_C Cuts at: 2171 3179 4335 2171 Size: 1008 1156 2958 Fragments arranged by size: 2958 1156 1008 ApoI r'AATT_y Cuts at: 1493 2431 2657 2809 4918 1493 Size: 938 226 152 2109 1697 Fragments arranged by size: 2109 1697 938 226 152 AseI AT'TA_AT Cuts at: 160 1032 3676 160 Size: 872 2644 1606 Fragments arranged by size: 2644 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5122 AvaI C'yCGr_G Cuts at: 1870 1943 2447 1870 Size: 73 504 4545 Fragments arranged by size: 4545 504 73 BamHI G'GATC_C Cuts at: 2452 2819 2452 Size: 367 4755 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2443 3898 618 Size: 325 217 627 656 1455 1842 Fragments arranged by size: 1842 1455 656 627 325 217 BanII G_rGCy'C Cuts at: 1391 2441 1391 Size: 1050 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2001 928 Size: 971 102 4049 Fragments arranged by size: 4049 971 102 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3313 3347 1903 Size: 34 1376 34 3678 Fragments arranged by size: 3678 1376 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3014 4224 3014 Size: 1210 3912 BclI T'GATC_A Cuts at: 1548 1548 Size: 5122 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3733 136 Size: 107 122 71 631 605 2061 1525 Fragments arranged by size: 2061 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5117 5117 Size: 5122 BlpI GC'TnA_GC Cuts at: 2539 2539 Size: 5122 Bme1580I G_kGCm'C Cuts at: 1790 2175 2250 3183 4339 1790 Size: 385 75 933 1156 2573 Fragments arranged by size: 2573 1156 933 385 75 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5122 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2853 3811 442 Size: 416 53 83 399 24 1436 958 1753 Fragments arranged by size: 1753 1436 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5122 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3782 1239 Size: 2543 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3247 4112 4410 3247 Size: 865 298 3959 Fragments arranged by size: 3959 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3785 882 Size: 617 2286 2219 Fragments arranged by size: 2286 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2245 2287 2913 493 Size: 711 1041 42 626 2702 Fragments arranged by size: 2702 1041 711 626 42 BsaBI GATnn'nnATC Cuts at: 5116 5116 Size: 5122 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2929 3311 3946 275 Size: 53 83 186 936 1396 382 635 1451 Fragments arranged by size: 1451 1396 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3555 3953 4227 4374 4785 3555 Size: 398 274 147 411 3892 Fragments arranged by size: 3892 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4897 Fragments arranged by size: 4897 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2243 1186 Size: 1057 4065 BseYI C'CCAG_C Cuts at: 4325 4821 4325 Size: 496 4626 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4549 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2572 3333 4361 1540 Size: 275 186 571 761 1028 2301 Fragments arranged by size: 2301 1028 761 571 275 186 BsiHKAI G_wGCw'C Cuts at: 2175 3183 3268 4339 2175 Size: 1008 85 1071 2958 Fragments arranged by size: 2958 1071 1008 85 BsmI GAATG_Cn' Cuts at: 2633 2726 2633 Size: 93 5029 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2175 2250 2441 3183 3268 4339 1391 Size: 399 385 75 191 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 385 191 85 75 BspEI T'CCGG_A Cuts at: 4785 4785 Size: 5122 BspHI T'CATG_A Cuts at: 2193 3011 4741 2193 Size: 818 1730 2574 Fragments arranged by size: 2574 1730 818 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2104 2477 844 Size: 1260 373 3489 Fragments arranged by size: 3489 1260 373 BsrBI CCG'CTC Cuts at: 1394 1823 3009 1394 Size: 429 1186 3507 Fragments arranged by size: 3507 1186 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2425 3617 3791 65 Size: 1183 1177 1192 174 1396 Fragments arranged by size: 1396 1192 1183 1177 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3766 3953 1700 Size: 110 1956 187 2869 Fragments arranged by size: 2869 1956 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5122 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5122 BssSI C'ACGA_G Cuts at: 3182 4194 3182 Size: 1012 4110 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5122 BstBI TT'CG_AA Cuts at: 3926 3926 Size: 5122 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5122 BstYI r'GATC_y Cuts at: 1972 2452 2819 3204 3221 4673 4759 4771 5117 Size: 480 367 385 17 1452 86 12 346 Cuts at: 5117 1972 Size: 1977 Fragments arranged by size: 1977 1452 480 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4570 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4383 Fragments arranged by size: 4383 683 56 BtsI GCAGTG_nn' Cuts at: 2638 3432 3452 2638 Size: 794 20 4308 Fragments arranged by size: 4308 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5122 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5087 DraI TTT'AAA Cuts at: 2426 2426 Size: 5122 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4129 809 Size: 507 2813 1802 Fragments arranged by size: 2813 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2569 3458 5087 Size: 54 1425 50 275 18 739 889 1629 Cuts at: 5087 8 Size: 43 Fragments arranged by size: 1629 1425 889 739 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2569 1537 Size: 275 757 4090 Fragments arranged by size: 4090 757 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3052 1353 Size: 1699 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3709 4083 4229 387 Size: 1064 138 2120 374 146 1280 Fragments arranged by size: 2120 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3185 3782 4569 4837 1239 Size: 393 1553 597 787 268 1524 Fragments arranged by size: 1553 1524 787 597 393 268 EcoRI G'AATT_C Cuts at: 2431 2809 2431 Size: 378 4744 EcoRV GAT'ATC Cuts at: 2392 2392 Size: 5122 FspI TGC'GCA Cuts at: 3628 3628 Size: 5122 HaeII r_GCGC'y Cuts at: 1678 4269 1678 Size: 2591 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3763 3795 3837 3869 1373 Size: 32 135 32 2191 32 42 32 2626 Fragments arranged by size: 2626 2191 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2466 2712 669 Size: 988 281 528 246 3079 Fragments arranged by size: 3079 988 528 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5122 HpaI GTT'AAC Cuts at: 2712 2712 Size: 5122 KpnI G_GTAC'C Cuts at: 2447 2447 Size: 5122 MfeI C'AATT_G Cuts at: 2721 2721 Size: 5122 MluI A'CGCG_T Cuts at: 4998 4998 Size: 5122 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2205 4236 4420 1305 Size: 900 2031 184 2007 Fragments arranged by size: 2031 2007 900 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3300 Fragments arranged by size: 3300 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2132 2295 3080 3439 3598 518 Size: 648 966 163 785 359 159 2042 Fragments arranged by size: 2042 966 785 648 359 163 159 MspA1I CmG'CkG Cuts at: 1606 2110 2538 3215 4363 4608 1606 Size: 504 428 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 504 428 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4570 NdeI CA'TA_TG Cuts at: 387 387 Size: 5122 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5122 NotI GC'GGCC_GC Cuts at: 2569 2569 Size: 5122 NspI r_CATG'y Cuts at: 1132 1229 2480 4025 1132 Size: 97 1251 1545 2229 Fragments arranged by size: 2229 1545 1251 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5122 PciI A'CATG_T Cuts at: 4021 4021 Size: 5122 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5122 PmeI GTTT'AAAC Cuts at: 2426 2426 Size: 5122 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3191 3223 4704 4736 3191 Size: 32 1481 32 3577 Fragments arranged by size: 3577 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5122 PsiI TTA'TAA Cuts at: 2692 2692 Size: 5122 PstI C_TGCA'G Cuts at: 830 2115 2474 830 Size: 1285 359 3478 Fragments arranged by size: 3478 1285 359 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5122 PvuII CAG'CTG Cuts at: 2110 2110 Size: 5122 SalI G'TCGA_C Cuts at: 1936 2464 1936 Size: 528 4594 SbfI CC_TGCA'GG Cuts at: 2474 2474 Size: 5122 ScaI AGT'ACT Cuts at: 3370 5082 3370 Size: 1712 3410 SfcI C'TryA_G Cuts at: 826 1045 2111 2470 3605 4286 4477 826 Size: 219 1066 359 1135 681 191 1471 Fragments arranged by size: 1471 1135 1066 681 359 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5122 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5122 SmaI CCC'GGG Cuts at: 1872 2449 1872 Size: 577 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3226 4127 4389 820 Size: 197 926 1283 901 262 1553 Fragments arranged by size: 1553 1283 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2287 2913 493 Size: 1794 626 2702 Fragments arranged by size: 2702 1794 626 SpeI A'CTAG_T Cuts at: 152 152 Size: 5122 SphI G_CATG'C Cuts at: 1132 2480 1132 Size: 1348 3774 SspI AAT'ATT Cuts at: 5 52 3046 5 Size: 47 2994 2081 Fragments arranged by size: 2994 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5122 StyI C'CwwG_G Cuts at: 513 1065 2561 513 Size: 552 1496 3074 Fragments arranged by size: 3074 1496 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3149 3166 3319 3504 3949 325 Size: 1255 1569 17 153 185 445 1498 Fragments arranged by size: 1569 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3368 5080 96 Size: 274 80 33 51 1443 1391 1712 138 Fragments arranged by size: 1712 1443 1391 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5082 5114 5082 Size: 32 5090 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5122 XbaI T'CTAG_A Cuts at: 2458 2458 Size: 5122 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5122 XmaI C'CCGG_G Cuts at: 1870 2447 1870 Size: 577 4545 XmnI GAAnn'nnTTC Cuts at: 3251 3251 Size: 5122 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2930 276 Size: 53 83 186 936 1396 2468 Fragments arranged by size: 2468 1396 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI