################################## # NO CUT ENZYMES ( 75 enzymes) # ################################## AatII AccIII AclI AflII AgeI Aor51HI AscI AseI AsnI AviII AvrII BcgI BciVI BclI BfrI BglII BlnI BsaWI BseAI BsiWI BsmI BspDI BspEI BspHI BssHII Bst1107I BstBI BstZ17I ClaI CpoI DraI Eco47III EcoT22I FbaI FseI FspI HindIII HpaI MfeI MluI MroI MunI NaeI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmeI PshBI Psp1406I PvuI RcaI RsrII SalI SapI SbfI ScaI SfiI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspI SwaI XbaI XhoI ################################### # ONE CUT ENZYMES ( 31 enzymes) # ################################### Acc65I 245 GGTACC G^GTACC AccI 3005 GTCTAC GT^[AC][GT]AC AcsI 277 GAATTC [GA]^AATT[CT] AhdI 722 GACCCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 2206 GTGCAC G^TGCAC AocI 3423 CCTGAGG CC^T[ACGT]AGG ApaLI 2206 GTGCAC G^TGCAC ApoI 277 GAATTC [GA]^AATT[CT] Asp700I 579 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 245 GGTACC G^GTACC AspEI 722 GACCCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BsiEI 339 CGGCCG CG[GA][CT]^CG BsmBI 1679 GAGACG CGTCTC(1/5) Bsp1407I 2758 TGTACA T^GTACA BsrGI 2758 TGTACA T^GTACA BssSI 364 CTCGTG CACGAG(-5/-1) BstAPI 1137 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Bsu36I 3423 CCTGAGG CC^T[ACGT]AGG DrdI 3266 GACTCAGGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 339 CGGCCG C^GGCCG Eam1105I 722 GACCCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 339 CGGCCG C^GGCCG Eco52I 339 CGGCCG C^GGCCG Eco81I 3423 CCTGAGG CC^T[ACGT]AGG EcoRI 277 GAATTC G^AATTC EcoRV 2376 GATATC GAT^ATC KpnI 245 GGTACC GGTAC^C SexAI 3653 ACCAGGT A^CC[AT]GGT SspBI 2758 TGTACA T^GTACA Tsp509I 278 AATT ^AATT XmnI 579 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### BamHI 227 GGATCC G^GATCC 3633 GGATCC BbeI 2396 GGCGCC GGCGC^C 2466 GGCGCC BbrPI 760 CACGTG CAC^GTG 1578 CACGTG BlpI 942 GCTCAGC GC^T[ACGT]AGC 1596 GCTCAGC Bpu1102I 942 GCTCAGC GC^T[ACGT]AGC 1596 GCTCAGC BsaBI 1040 GATCTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2290 GATGCCAATC BsaI 1279 GAGACC GGTCTC(1/5) 3109 GGTCTC BspLU11I 1246 ACATGT A^CATGT 2755 ACATGT BsrDI 2715 CATTGC GCAATG(2/0) 186 GCAATG BsrFI 849 GCCGGT [GA]^CCGG[CT] 1722 GCCGGT CelII 942 GCTCAGC GC^T[ACGT]AGC 1596 GCTCAGC Cfr10I 849 GCCGGT [GA]^CCGG[CT] 1722 GCCGGT HincII 472 GTCAAC GT[CT]^[GA]AC 1726 GTCAAC HindII 472 GTCAAC GT[CT]^[GA]AC 1726 GTCAAC KasI 2396 GGCGCC G^GCGCC 2466 GGCGCC MamI 1040 GATCTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2290 GATGCCAATC MseI 1687 TTAA T^TAA 2839 TTAA NarI 2396 GGCGCC GG^CGCC 2466 GGCGCC PmaCI 760 CACGTG CAC^GTG 1578 CACGTG PmlI 760 CACGTG CAC^GTG 1578 CACGTG PshAI 1228 GACAACGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 1695 GACATGCGTC SfoI 2396 GGCGCC GGC^GCC 2466 GGCGCC Tru9I 1687 TTAA T^TAA 2839 TTAA ######################################### # 3 OR MORE CUT ENZYMES ( 129 enzymes) # ######################################### AccII AciI AcyI AfaI AflIII AluI AlwI AlwNI ApaI AspHI AspI AvaI AvaII BalI BanI BanII BbsI BbvCI BbvI BcnI BfaI BglI BmyI BpmI BpuAI BsaAI BsaHI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrI BssKI BstEII BstF5I BstNI BstPI BstUI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI EcoNI EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I KspI MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflFI PflMI PleI PpuMI PstI PvuII RsaI SacI SacII Sau3AI Sau96I ScrFI SfaNI SfcI SmaI StuI StyI TaqI TfiI TseI Tsp45I TspRI Tth111I TthHB8I Van91I XcmI XhoII XmaCI XmaI