(Linear) (Six-base) MAPSORT of: KIEE4595.seq Check: 4085 from: 1 to: 3723 KIEE4595.orf With 182 enzymes: SgfI * March 22, 2012 18:27 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 293 1944 3723 Size: 293 1651 1779 Fragments arranged by size: 1779 1651 293 AccI GT'mk_AC Cuts at: 0 3006 3723 Size: 3006 717 Acc65I G'GTAC_C Cuts at: 0 245 3723 Size: 245 3478 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 74 856 1242 3146 3155 3417 3638 3723 Size: 74 782 386 1904 9 262 221 85 Fragments arranged by size: 1904 782 386 262 221 85 74 9 AflIII A'CryG_T Cuts at: 0 1246 2723 2755 3723 Size: 1246 1477 32 968 Fragments arranged by size: 1477 1246 968 32 AhdI GACnn_n'nnGTC Cuts at: 0 727 3723 Size: 727 2996 AleI CACnn'nnGTG Cuts at: 0 828 2248 2588 2687 3723 Size: 828 1420 340 99 1036 Fragments arranged by size: 1420 1036 828 340 99 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 199 1015 3723 Size: 199 816 2708 Fragments arranged by size: 2708 816 199 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 553 585 3723 Size: 553 32 3138 Fragments arranged by size: 3138 553 32 AlwNI CAG_nnn'CTG Cuts at: 0 505 642 834 1143 1329 1959 2358 3032 Size: 505 137 192 309 186 630 399 674 Cuts at: 3032 3723 Size: 691 Fragments arranged by size: 691 674 630 505 399 309 192 186 137 ApaI G_GGCC'C Cuts at: 0 108 217 359 3723 Size: 108 109 142 3364 Fragments arranged by size: 3364 142 109 108 ApaLI G'TGCA_C Cuts at: 0 2206 3723 Size: 2206 1517 ApoI r'AATT_y Cuts at: 0 277 3723 Size: 277 3446 AvaI C'yCGr_G Cuts at: 0 92 240 358 800 1376 1529 1674 2026 Size: 92 148 118 442 576 153 145 352 Cuts at: 2026 2307 2594 3171 3476 3723 Size: 281 287 577 305 247 Fragments arranged by size: 577 576 442 352 305 287 281 247 153 148 145 118 92 BamHI G'GATC_C Cuts at: 0 227 3633 3723 Size: 227 3406 90 Fragments arranged by size: 3406 227 90 BanI G'GyrC_C Cuts at: 0 245 433 2176 2396 2466 3723 Size: 245 188 1743 220 70 1257 Fragments arranged by size: 1743 1257 245 220 188 70 BanII G_rGCy'C Cuts at: 0 108 217 359 365 571 625 1421 1791 Size: 108 109 142 6 206 54 796 370 Cuts at: 1791 1889 1906 2109 3382 3723 Size: 98 17 203 1273 341 Fragments arranged by size: 1273 796 370 341 206 203 142 109 108 98 54 17 6 BbeI G_GCGC'C Cuts at: 0 2400 2470 3723 Size: 2400 70 1253 Fragments arranged by size: 2400 1253 70 BbsI GAAGACnn'nnnn_ Cuts at: 0 932 3143 3304 3626 3723 Size: 932 2211 161 322 97 Fragments arranged by size: 2211 932 322 161 97 BbvCI CC'TCA_GC Cuts at: 0 307 3281 3706 3723 Size: 307 2974 425 17 Fragments arranged by size: 2974 425 307 17 BglI GCCn_nnn'nGGC Cuts at: 0 338 2029 2422 3723 Size: 338 1691 393 1301 Fragments arranged by size: 1691 1301 393 338 BlpI GC'TnA_GC Cuts at: 0 943 1597 3723 Size: 943 654 2126 Fragments arranged by size: 2126 943 654 Bme1580I G_kGCm'C Cuts at: 0 108 217 359 761 871 2179 2210 2232 Size: 108 109 142 402 110 1308 31 22 Cuts at: 2232 2595 3521 3723 Size: 363 926 202 Fragments arranged by size: 1308 926 402 363 202 142 110 109 108 31 22 BmgBI CAC'GTC Cuts at: 0 2205 3723 Size: 2205 1518 BmrI ACTGGGnnnn_n' Cuts at: 0 2331 2341 3723 Size: 2331 10 1382 Fragments arranged by size: 2331 1382 10 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 156 400 958 3306 3723 Size: 156 244 558 2348 417 Fragments arranged by size: 2348 558 417 244 156 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 691 701 1780 2218 2456 2576 2896 2930 Size: 691 10 1079 438 238 120 320 34 Cuts at: 2930 3146 3578 3723 Size: 216 432 145 Fragments arranged by size: 1079 691 438 432 320 238 216 145 120 34 10 Bpu10I CC'TnA_GC Cuts at: 0 19 307 816 3281 3706 3723 Size: 19 288 509 2465 425 17 Fragments arranged by size: 2465 509 425 288 19 17 BsaI GGTCTCn'nnnn_ Cuts at: 0 1273 3115 3723 Size: 1273 1842 608 Fragments arranged by size: 1842 1273 608 BsaAI yAC'GTr Cuts at: 0 468 762 1580 3723 Size: 468 294 818 2143 Fragments arranged by size: 2143 818 468 294 BsaBI GATnn'nnATC Cuts at: 0 1044 2294 3723 Size: 1044 1250 1429 Fragments arranged by size: 1429 1250 1044 BsaHI Gr'CG_yC Cuts at: 0 655 1682 2397 2467 3273 3723 Size: 655 1027 715 70 806 450 Fragments arranged by size: 1027 806 715 655 450 70 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 313 343 553 583 775 805 2471 2501 Size: 313 30 210 30 192 30 1666 30 Cuts at: 2501 3337 3367 3451 3481 3723 Size: 836 30 84 30 242 Fragments arranged by size: 1666 836 313 242 210 192 84 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1168 2359 3211 3358 3475 3723 Size: 1168 1191 852 147 117 248 Fragments arranged by size: 1191 1168 852 248 147 117 BseYI C'CCAG_C Cuts at: 0 454 572 663 1125 1369 3086 3440 3723 Size: 454 118 91 462 244 1717 354 283 Fragments arranged by size: 1717 462 454 354 283 244 118 91 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 271 1642 2140 2265 3607 3723 Size: 271 1371 498 125 1342 116 Fragments arranged by size: 1371 1342 498 271 125 116 BsiEI CG_ry'CG Cuts at: 0 342 3723 Size: 342 3381 BsiHKAI G_wGCw'C Cuts at: 0 365 625 878 989 1522 1889 1906 2210 Size: 365 260 253 111 533 367 17 304 Cuts at: 2210 2525 2675 3382 3723 Size: 315 150 707 341 Fragments arranged by size: 707 533 367 365 341 315 304 260 253 150 111 17 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1673 3723 Size: 1673 2050 Bsp1286I G_dGCh'C Cuts at: 0 108 217 359 365 571 625 761 871 Size: 108 109 142 6 206 54 136 110 Cuts at: 871 878 989 1421 1522 1791 1889 1906 2109 Size: 7 111 432 101 269 98 17 203 Cuts at: 2109 2179 2210 2232 2525 2595 2675 3382 3521 Size: 70 31 22 293 70 80 707 139 Cuts at: 3521 3723 Size: 202 Fragments arranged by size: 707 432 293 269 206 203 202 142 139 136 111 110 109 108 101 98 80 70 70 54 31 22 17 7 6 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 293 993 1873 1944 3723 Size: 293 700 880 71 1779 Fragments arranged by size: 1779 880 700 293 71 BsrBI CCG'CTC Cuts at: 0 344 3366 3419 3723 Size: 344 3022 53 304 Fragments arranged by size: 3022 344 304 53 BsrDI GCAATG_nn' Cuts at: 0 193 2714 3723 Size: 193 2521 1009 Fragments arranged by size: 2521 1009 193 BsrFI r'CCGG_y Cuts at: 0 849 1722 3723 Size: 849 873 2001 Fragments arranged by size: 2001 873 849 BsrGI T'GTAC_A Cuts at: 0 2758 3723 Size: 2758 965 BssSI C'ACGA_G Cuts at: 0 364 3723 Size: 364 3359 BstAPI GCAn_nnn'nTGC Cuts at: 0 1143 3723 Size: 1143 2580 BstEII G'GTnAC_C Cuts at: 0 441 1005 1965 3723 Size: 441 564 960 1758 Fragments arranged by size: 1758 960 564 441 BstXI CCAn_nnnn'nTGG Cuts at: 0 1614 2689 3124 3723 Size: 1614 1075 435 599 Fragments arranged by size: 1614 1075 599 435 BstYI r'GATC_y Cuts at: 0 227 1039 2646 2655 3633 3723 Size: 227 812 1607 9 978 90 Fragments arranged by size: 1607 978 812 227 90 9 Bsu36I CC'TnA_GG Cuts at: 0 3424 3723 Size: 3424 299 BtgI C'CryG_G Cuts at: 0 437 930 1102 1148 1970 2049 2180 2867 Size: 437 493 172 46 822 79 131 687 Cuts at: 2867 3383 3723 Size: 516 340 Fragments arranged by size: 822 687 516 493 437 340 172 131 79 46 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 326 2071 3723 Size: 326 1745 1652 Fragments arranged by size: 1745 1652 326 BtsI GCAGTG_nn' Cuts at: 0 183 823 835 903 997 1666 2157 2583 Size: 183 640 12 68 94 669 491 426 Cuts at: 2583 3723 Size: 1140 Fragments arranged by size: 1140 669 640 491 426 183 94 68 12 DraIII CAC_nnn'GTG Cuts at: 0 863 2868 2961 3723 Size: 863 2005 93 762 Fragments arranged by size: 2005 863 762 93 DrdI GACnn_nn'nnGTC Cuts at: 0 3272 3723 Size: 3272 451 EaeI y'GGCC_r Cuts at: 0 339 739 843 1652 1769 1851 3041 3659 Size: 339 400 104 809 117 82 1190 618 Cuts at: 3659 3723 Size: 64 Fragments arranged by size: 1190 809 618 400 339 117 104 82 64 EagI C'GGCC_G Cuts at: 0 339 3723 Size: 339 3384 EarI CTCTTCn'nnn_ Cuts at: 0 589 2755 3311 3723 Size: 589 2166 556 412 Fragments arranged by size: 2166 589 556 412 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1709 1794 3723 Size: 1709 85 1929 Fragments arranged by size: 1929 1709 85 EcoICRI GAG'CTC Cuts at: 0 363 623 1887 1904 3380 3723 Size: 363 260 1264 17 1476 343 Fragments arranged by size: 1476 1264 363 343 260 17 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 74 691 701 856 1242 1780 2218 2456 Size: 74 617 10 155 386 538 438 238 Cuts at: 2456 2576 2896 2930 3146 3155 3417 3578 3638 Size: 120 320 34 216 9 262 161 60 Cuts at: 3638 3723 Size: 85 Fragments arranged by size: 617 538 438 386 320 262 238 216 161 155 120 85 74 60 34 10 9 EcoNI CCTnn'n_nnAGG Cuts at: 0 269 751 2539 2831 3723 Size: 269 482 1788 292 892 Fragments arranged by size: 1788 892 482 292 269 EcoO109I rG'GnC_Cy Cuts at: 0 105 137 214 261 355 675 721 977 Size: 105 32 77 47 94 320 46 256 Cuts at: 977 1342 1373 2238 2637 2901 2934 3083 3166 Size: 365 31 865 399 264 33 149 83 Cuts at: 3166 3249 3568 3595 3603 3723 Size: 83 319 27 8 120 Fragments arranged by size: 865 399 365 320 319 264 256 149 120 105 94 83 83 77 47 46 33 32 31 27 8 EcoRI G'AATT_C Cuts at: 0 277 3723 Size: 277 3446 EcoRV GAT'ATC Cuts at: 0 2378 3723 Size: 2378 1345 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1299 2907 3723 Size: 1299 1608 816 Fragments arranged by size: 1608 1299 816 HaeII r_GCGC'y Cuts at: 0 1883 2400 2438 2470 3723 Size: 1883 517 38 32 1253 Fragments arranged by size: 1883 1253 517 38 32 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 352 384 713 745 1326 1358 2362 2394 Size: 352 32 329 32 581 32 1004 32 Cuts at: 2394 2471 2503 2815 2847 3014 3046 3295 3327 Size: 77 32 312 32 167 32 249 32 Cuts at: 3327 3451 3483 3699 3723 Size: 124 32 216 24 Fragments arranged by size: 1004 581 352 329 312 249 216 167 124 77 32 32 32 32 32 32 32 32 32 24 HincII GTy'rAC Cuts at: 0 474 1728 3723 Size: 474 1254 1995 Fragments arranged by size: 1995 1254 474 KasI G'GCGC_C Cuts at: 0 2396 2466 3723 Size: 2396 70 1257 Fragments arranged by size: 2396 1257 70 KpnI G_GTAC'C Cuts at: 0 249 3723 Size: 249 3474 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1223 1538 3293 3723 Size: 1223 315 1755 430 Fragments arranged by size: 1755 1223 430 315 MscI TGG'CCA Cuts at: 0 741 845 1654 1853 3043 3723 Size: 741 104 809 199 1190 680 Fragments arranged by size: 1190 809 741 680 199 104 MslI CAynn'nnrTG Cuts at: 0 828 1192 2248 2588 2687 3524 3723 Size: 828 364 1056 340 99 837 199 Fragments arranged by size: 1056 837 828 364 340 199 99 MspA1I CmG'CkG Cuts at: 0 947 1104 1140 1614 1972 2051 2071 2102 Size: 947 157 36 474 358 79 20 31 Cuts at: 2102 2154 2182 2554 3144 3261 3444 3723 Size: 52 28 372 590 117 183 279 Fragments arranged by size: 947 590 474 372 358 279 183 157 117 79 52 36 31 28 20 NarI GG'CG_CC Cuts at: 0 2397 2467 3723 Size: 2397 70 1256 Fragments arranged by size: 2397 1256 70 NcoI C'CATG_G Cuts at: 0 437 930 1148 3723 Size: 437 493 218 2575 Fragments arranged by size: 2575 493 437 218 NspI r_CATG'y Cuts at: 0 1250 1700 2316 2759 3723 Size: 1250 450 616 443 964 Fragments arranged by size: 1250 964 616 450 443 PasI CC'CwG_GG Cuts at: 0 6 2937 3514 3599 3723 Size: 6 2931 577 85 124 Fragments arranged by size: 2931 577 124 85 6 PciI A'CATG_T Cuts at: 0 1246 2755 3723 Size: 1246 1509 968 Fragments arranged by size: 1509 1246 968 PflMI CCAn_nnn'nTGG Cuts at: 0 863 888 2358 2961 3723 Size: 863 25 1470 603 762 Fragments arranged by size: 1470 863 762 603 25 PfoI T'CCnGG_A Cuts at: 0 717 2234 3723 Size: 717 1517 1489 Fragments arranged by size: 1517 1489 717 PmlI CAC'GTG Cuts at: 0 762 1580 3723 Size: 762 818 2143 Fragments arranged by size: 2143 818 762 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 553 585 3723 Size: 553 32 3138 Fragments arranged by size: 3138 553 32 PpuMI rG'GwC_Cy Cuts at: 0 261 721 977 1342 1373 2238 2637 3083 Size: 261 460 256 365 31 865 399 446 Cuts at: 3083 3166 3249 3568 3595 3603 3723 Size: 83 83 319 27 8 120 Fragments arranged by size: 865 460 446 399 365 319 261 256 120 83 83 31 27 8 PshAI GACnn'nnGTC Cuts at: 0 1232 1699 3723 Size: 1232 467 2024 Fragments arranged by size: 2024 1232 467 PspOMI G'GGCC_C Cuts at: 0 104 213 355 3723 Size: 104 109 142 3368 Fragments arranged by size: 3368 142 109 104 PstI C_TGCA'G Cuts at: 0 830 975 1004 2553 3723 Size: 830 145 29 1549 1170 Fragments arranged by size: 1549 1170 830 145 29 PvuII CAG'CTG Cuts at: 0 947 1140 1614 2154 2554 3144 3444 3723 Size: 947 193 474 540 400 590 300 279 Fragments arranged by size: 947 590 540 474 400 300 279 193 SacI G_AGCT'C Cuts at: 0 365 625 1889 1906 3382 3723 Size: 365 260 1264 17 1476 341 Fragments arranged by size: 1476 1264 365 341 260 17 SacII CC_GC'GG Cuts at: 0 1105 1973 2052 2183 3723 Size: 1105 868 79 131 1540 Fragments arranged by size: 1540 1105 868 131 79 SanDI GG'GwC_CC Cuts at: 0 261 977 1342 1373 2637 3083 3568 3595 Size: 261 716 365 31 1264 446 485 27 Cuts at: 3595 3723 Size: 128 Fragments arranged by size: 1264 716 485 446 365 261 128 31 27 SexAI A'CCwGG_T Cuts at: 0 3653 3723 Size: 3653 70 SfcI C'TryA_G Cuts at: 0 826 971 1000 2549 3007 3723 Size: 826 145 29 1549 458 716 Fragments arranged by size: 1549 826 716 458 145 29 SfoI GGC'GCC Cuts at: 0 2398 2468 3723 Size: 2398 70 1255 Fragments arranged by size: 2398 1255 70 SmaI CCC'GGG Cuts at: 0 94 1378 1676 2309 3723 Size: 94 1284 298 633 1414 Fragments arranged by size: 1414 1284 633 298 94 StuI AGG'CCT Cuts at: 0 59 298 688 746 1255 2032 3723 Size: 59 239 390 58 509 777 1691 Fragments arranged by size: 1691 777 509 390 239 59 58 StyI C'CwwG_G Cuts at: 0 437 930 1148 1274 2775 3053 3723 Size: 437 493 218 126 1501 278 670 Fragments arranged by size: 1501 670 493 437 278 218 126 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 895 1471 3338 3723 Size: 895 576 1867 385 Fragments arranged by size: 1867 895 576 385 TatI w'GTAC_w Cuts at: 0 2758 3723 Size: 2758 965 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 313 345 775 807 3337 3369 3704 3723 Size: 313 32 430 32 2530 32 335 19 Fragments arranged by size: 2530 430 335 313 32 32 32 19 Tth111I GACn'n_nGTC Cuts at: 0 1458 2275 3325 3723 Size: 1458 817 1050 398 Fragments arranged by size: 1458 1050 817 398 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1032 3051 3121 3723 Size: 1032 2019 70 602 Fragments arranged by size: 2019 1032 602 70 XmaI C'CCGG_G Cuts at: 0 92 1376 1674 2307 3723 Size: 92 1284 298 633 1416 Fragments arranged by size: 1416 1284 633 298 92 XmnI GAAnn'nnTTC Cuts at: 0 583 3723 Size: 583 3140 Enzymes that do cut: AarI AccI Acc65I AcuI AflIII AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AvaI BamHI BanI BanII BbeI BbsI BbvCI BglI BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BsaI BsaAI BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI HaeII Hin4I HincII KasI KpnI MmeI MscI MslI MspA1I NarI NcoI NspI PasI PciI PflMI PfoI PmlI PpiI PpuMI PshAI PspOMI PstI PvuII SacI SacII SanDI SexAI SfcI SfoI SmaI StuI StyI TaqII TatI TstI Tth111I XcmI XmaI XmnI Enzymes that do not cut: AatII AclI AfeI AflII AgeI AscI AseI AsiSI AvrII BaeI BcgI BciVI BclI BfrBI BglII BmtI BpuEI BsaWI BsiWI BsmI BspEI BspHI BssHII BstBI BstZ17I ClaI CspCI DraI FseI FspI FspAI HindIII HpaI MfeI MluI NaeI NdeI NgoMIV NheI NotI NruI NsiI PacI PmeI PsiI PspXI PsrI PvuI RsrII SalI SapI SbfI ScaI SfiI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI SwaI XbaI XhoI ZraI