################################## # NO CUT ENZYMES ( 203 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BbvI BcgI BciVI BclI BfrI BglI BglII BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstUI BstXI BstYI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I ClaI CpoI DdeI DpnII DraI DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FbaI Fnu4HI FokI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HpaI ItaI KasI KpnI Ksp632I KspI MaeII MamI MboI MfeI MflI MluI MluNI MroI MscI MslI MspA1I MunI MvnI MwoI NaeI NarI NcoI NdeI NdeII NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI Sau3AI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TaqI TfiI TseI TspRI Tth111I TthHB8I XbaI XcmI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AfaI 183 GTAC GT^AC AvaI 37 CCCGGG C^[CT]CG[GA]G AvrII 363 CCTAGG C^CTAGG BfaI 364 CTAG C^TAG BlnI 363 CCTAGG C^CTAGG BseRI 64 GAGGAG GAGGAG(10/8) BsiYI 54 CCAGGAAATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 54 CCAGGAAATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmAI 212 GTCTC GTCTC(1/5) BsmFI 40 GGGAC GGGAC(10/14) BsoBI 37 CCCGGG C^[CT]CG[GA]G DraII 32 AGGACCC [GA]G^G[ACGT]CC[CT] EcoO109I 32 AGGACCC [GA]G^G[ACGT]CC[CT] EcoT14I 363 CCTAGG C^C[AT][AT]GG HapII 38 CCGG C^CGG HindIII 311 AAGCTT A^AGCTT HinfI 210 GAGTC G^A[ACGT]TC HpaII 38 CCGG C^CGG HphI 397 GGTGA GGTGA(8/7) MaeI 364 CTAG C^TAG MboII 306 GAAGA GAAGA(8/7) MspI 38 CCGG C^CGG NlaIII 332 CATG CATG^ PflMI 54 CCAGGAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 210 GAGTC GAGTC(4/5) PpuMI 32 AGGACCC [GA]G^G[AT]CC[CT] RsaI 183 GTAC GT^AC SfaNI 114 GATGC GCATC(5/9) SmaI 37 CCCGGG CCC^GGG StuI 413 AGGCCT AGG^CCT StyI 363 CCTAGG C^C[AT][AT]GG Tsp509I 237 AATT ^AATT Van91I 54 CCAGGAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 37 CCCGGG C^CCGGG XmaI 37 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 10 enzymes) # ################################### AluI 312 AGCT AG^CT 340 AGCT AvaII 33 GGACC G^G[AT]CC 436 GGACC BcnI 37 CCCGG CC^[GC]GG 38 CCGGG Cfr13I 33 GGACC G^G[ACGT]CC 436 GGACC HaeIII 361 GGCC GG^CC 414 GGCC MseI 239 TTAA T^TAA 380 TTAA NciI 37 CCCGG CC^[GC]GG 38 CCGGG Sau96I 33 GGACC G^G[ACGT]CC 436 GGACC SfcI 9 CTGTAG C^T[GA][CT]AG 143 CTGTAG Tru9I 239 TTAA T^TAA 380 TTAA ######################################### # 3 OR MORE CUT ENZYMES ( 10 enzymes) # ######################################### BsaJI BssKI BstNI EcoRII MaeIII MnlI MvaI NlaIV ScrFI Tsp45I