################################## # NO CUT ENZYMES ( 143 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII Alw44I Aor51HI ApaLI AscI AseI AsnI Asp718I AspHI AspI AvaI AviII AvrII BbeI BbrPI BbsI BcgI BclI BcnI BfrI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BseAI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstZ17I CelII CfoI ClaI CpoI DraI DraIII DrdI EagI EclXI Eco47III Eco52I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HindIII HpaI KasI KpnI KspI MaeII MamI MfeI MluI MroI MspA1I MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PmaCI PmeI PmlI PshBI Psp1406I PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tth111I TthHB8I Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AcsI 31 AAATTT [GA]^AATT[CT] AgeI 362 ACCGGT A^CCGGT AhdI 764 GACTTGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwNI 509 CAGGCACTG CAG[ACGT][ACGT][ACGT]^CTG AocI 873 CCTGAGG CC^T[ACGT]AGG ApaI 896 GGGCCC GGGCC^C ApoI 31 AAATTT [GA]^AATT[CT] Asp700I 855 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 764 GACTTGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaII 549 GGTCC G^G[AT]CC BamHI 638 GGATCC G^GATCC BanI 287 GGTGCC G^G[CT][GA]CC BanII 896 GGGCCC G[GA]GC[CT]^C BbvCI 912 CCTCAGC CCTCAGC(-5/-2) BciVI 330 GGATAC GTATCC(6/5) BfaI 244 CTAG C^TAG BmyI 896 GGGCCC G[AGT]GC[ACT]^C BsaI 741 GGTCTC GGTCTC(1/5) BsaWI 362 ACCGGT [AT]^CCGG[AT] BsgI 388 CTGCAC GTGCAG(16/14) Bsp1286I 896 GGGCCC G[AGT]GC[ACT]^C BspHI 404 TCATGA T^CATGA BspMI 125 ACCTGC ACCTGC(4/8) BsrI 342 ACTGG ACTGG(1/-1) BstEII 840 GGTCACC G^GT[ACGT]ACC BstPI 840 GGTCACC G^GT[ACGT]ACC BstXI 98 CCATGTACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 873 CCTGAGG CC^T[ACGT]AGG Cac8I 562 GCCTGC GC[ACGT]^[ACGT]GC Eam1105I 764 GACTTGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 873 CCTGAGG CC^T[ACGT]AGG EcoNI 291 CCTCACTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 840 GGTCACC G^GT[ACGT]ACC MaeI 244 CTAG C^TAG NcoI 836 CCATGG C^CATGG PinAI 362 ACCGGT A^CCGGT PpuMI 548 AGGTCCT [GA]G^G[AT]CC[CT] PshAI 925 GACTCTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 67 CTGCAG CTGCA^G RcaI 404 TCATGA T^CATGA SexAI 104 ACCTGGT A^CC[AT]GGT SfiI 345 GGCCGTGATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmlI 553 CTCAAG C^T[CT][GA]AG TfiI 519 GATTC G^A[AT]TC XcmI 605 CCACGGCACTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 855 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### AciI 523 CCGC CCGC(-3/-1) 818 CCGC BalI 371 TGGCCA TGG^CCA 602 TGGCCA BbvI 69 GCAGC GCAGC(8/12) 809 GCAGC BglI 346 GCCGTGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 416 GCCTCTGTGGC BsmAI 664 GTCTC GTCTC(1/5) 742 GTCTC BsmFI 45 GGGAC GGGAC(10/14) 232 GTCCC BsrFI 362 ACCGGT [GA]^CCGG[CT] 540 ACCGGC BstF5I 252 CATCC GGATG(2/0) 885 GGATG BstYI 638 GGATCC [GA]^GATC[CT] 724 GGATCT Cfr10I 362 ACCGGT [GA]^CCGG[CT] 540 ACCGGC DsaI 605 CCACGG C^C[GA][CT]GG 836 CCATGG EarI 643 CTCTTC CTCTTC(1/4) 489 GAAGAG EcoT14I 692 CCAAGG C^C[AT][AT]GG 836 CCATGG Fnu4HI 69 GCAGC GC^[ACGT]GC 809 GCAGC FokI 252 CATCC GGATG(9/13) 885 GGATG HapII 363 CCGG C^CGG 541 CCGG HincII 205 GTTGAC GT[CT]^[GA]AC 495 GTTGAC HindII 205 GTTGAC GT[CT]^[GA]AC 495 GTTGAC HpaII 363 CCGG C^CGG 541 CCGG HphI 729 TCACC GGTGA(8/7) 842 TCACC ItaI 69 GCAGC GC^[ACGT]GC 809 GCAGC Ksp632I 643 CTCTTC CTCTTC(1/4) 489 GAAGAG MflI 638 GGATCC [GA]^GATC[CT] 724 GGATCT MluNI 371 TGGCCA TGG^CCA 602 TGGCCA MscI 371 TGGCCA TGG^CCA 602 TGGCCA MseI 310 TTAA T^TAA 673 TTAA MspI 363 CCGG C^CGG 541 CCGG MwoI 346 GCCGTGATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 416 GCCTCTGTGGC StyI 692 CCAAGG C^C[AT][AT]GG 836 CCATGG Tru9I 310 TTAA T^TAA 673 TTAA TseI 69 GCAGC G^C[AT]GC 809 GCAGC Tsp509I 23 AATT ^AATT 32 AATT XhoII 638 GGATCC [GA]^GATC[CT] 724 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 36 enzymes) # ######################################### AfaI AluI AlwI BsaJI BseRI BsiYI BslI BssKI BstNI Cfr13I DdeI DpnII DraII EaeI EcoO109I EcoRII HaeIII HinfI MaeIII MboI MboII MnlI MslI MvaI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI Tsp45I TspRI