(Circular) (Six-base) MAPSORT of: pFN21AE5492.seq Check: 1983 from: 1 to: 5740 pFN21AE5492 5740 bp With 182 enzymes: SgfI * January 30, 2014 17:21 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3550 278 Size: 53 83 186 936 2014 2468 Fragments arranged by size: 2468 2014 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3083 1937 Size: 1146 4594 Acc65I G'GTAC_C Cuts at: 3061 3061 Size: 5740 AclI AA'CG_TT Cuts at: 1563 3867 4240 1563 Size: 2304 373 3063 Fragments arranged by size: 3063 2304 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2874 3803 5187 5455 1632 Size: 1242 929 1384 268 1917 Fragments arranged by size: 1917 1384 1242 929 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5543 AflIII A'CryG_T Cuts at: 1203 4639 5616 1203 Size: 3436 977 1327 Fragments arranged by size: 3436 1327 977 AgeI A'CCGG_T Cuts at: 4571 4571 Size: 5740 AhdI GACnn_n'nnGTC Cuts at: 4469 4469 Size: 5740 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5740 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5740 AlwNI CAG_nnn'CTG Cuts at: 2020 3483 5055 2020 Size: 1463 1572 2705 Fragments arranged by size: 2705 1572 1463 ApaLI G'TGCA_C Cuts at: 3797 4953 3797 Size: 1156 4584 ApoI r'AATT_y Cuts at: 1493 2099 3049 3275 3427 5536 1493 Size: 606 950 226 152 2109 1697 Fragments arranged by size: 2109 1697 950 606 226 152 AseI AT'TA_AT Cuts at: 160 1032 4294 160 Size: 872 3262 1606 Fragments arranged by size: 3262 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5740 AvaI C'yCGr_G Cuts at: 1870 1943 3065 1870 Size: 73 1122 4545 Fragments arranged by size: 4545 1122 73 AvrII C'CTAG_G Cuts at: 2870 2870 Size: 5740 BamHI G'GATC_C Cuts at: 2125 3070 3437 2125 Size: 945 367 4428 Fragments arranged by size: 4428 945 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3061 4516 618 Size: 325 217 627 1274 1455 1842 Fragments arranged by size: 1842 1455 1274 627 325 217 BanII G_rGCy'C Cuts at: 1391 3059 1391 Size: 1668 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2882 928 Size: 971 983 3786 Fragments arranged by size: 3786 983 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 1999 2021 2033 3931 3965 1903 Size: 34 50 12 22 12 1898 34 3678 Fragments arranged by size: 3678 1898 50 34 34 22 12 12 BciVI GTATCCnnnnn_n' Cuts at: 2982 3632 4842 2982 Size: 650 1210 3880 Fragments arranged by size: 3880 1210 650 BclI T'GATC_A Cuts at: 1548 1548 Size: 5740 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2490 4351 136 Size: 107 122 71 631 605 818 1861 1525 Fragments arranged by size: 1861 1525 818 631 605 122 107 71 BglII A'GATC_T Cuts at: 2367 5735 2367 Size: 3368 2372 BlpI GC'TnA_GC Cuts at: 3157 3157 Size: 5740 Bme1580I G_kGCm'C Cuts at: 1790 3801 4957 1790 Size: 2011 1156 2573 Fragments arranged by size: 2573 2011 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5740 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3471 4429 442 Size: 416 53 83 399 24 2054 958 1753 Fragments arranged by size: 2054 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5740 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4400 1239 Size: 3161 2579 Bpu10I CC'TnA_GC Cuts at: 2613 2613 Size: 5740 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2989 3865 4730 5028 2989 Size: 876 865 298 3701 Fragments arranged by size: 3701 876 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2799 4403 882 Size: 617 1300 1604 2219 Fragments arranged by size: 2219 1604 1300 617 BsaAI yAC'GTr Cuts at: 493 1204 2346 3531 493 Size: 711 1142 1185 2702 Fragments arranged by size: 2702 1185 1142 711 BsaBI GATnn'nnATC Cuts at: 5734 5734 Size: 5740 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3547 3929 4564 275 Size: 53 83 186 936 2014 382 635 1451 Fragments arranged by size: 2014 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2128 4173 4571 4845 4992 5403 2128 Size: 2045 398 274 147 411 2465 Fragments arranged by size: 2465 2045 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5515 Fragments arranged by size: 5515 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5740 BseYI C'CCAG_C Cuts at: 4943 5439 4943 Size: 496 5244 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2557 1163 Size: 573 821 4346 Fragments arranged by size: 4346 821 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3190 3951 4979 1540 Size: 275 186 1189 761 1028 2301 Fragments arranged by size: 2301 1189 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3801 3886 4957 3801 Size: 85 1071 4584 Fragments arranged by size: 4584 1071 85 BsmI GAATG_Cn' Cuts at: 2536 2754 3251 3344 2536 Size: 218 497 93 4932 Fragments arranged by size: 4932 497 218 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3059 3801 3886 4957 1391 Size: 399 1269 742 85 1071 2174 Fragments arranged by size: 2174 1269 1071 742 399 85 BspEI T'CCGG_A Cuts at: 2128 5403 2128 Size: 3275 2465 BspHI T'CATG_A Cuts at: 2472 3629 5359 2472 Size: 1157 1730 2853 Fragments arranged by size: 2853 1730 1157 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2202 2819 3095 844 Size: 1358 617 276 3489 Fragments arranged by size: 3489 1358 617 276 BsrBI CCG'CTC Cuts at: 1394 1823 2593 3627 1394 Size: 429 770 1034 3507 Fragments arranged by size: 3507 1034 770 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2424 4235 4409 65 Size: 1183 1176 1811 174 1396 Fragments arranged by size: 1811 1396 1183 1176 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4384 4571 1700 Size: 110 2574 187 2869 Fragments arranged by size: 2869 2574 187 110 BsrGI T'GTAC_A Cuts at: 96 2841 96 Size: 2745 2995 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5740 BssSI C'ACGA_G Cuts at: 3800 4812 3800 Size: 1012 4728 BstAPI GCAn_nnn'nTGC Cuts at: 794 2020 794 Size: 1226 4514 BstBI TT'CG_AA Cuts at: 4544 4544 Size: 5740 BstEII G'GTnAC_C Cuts at: 1184 2176 1184 Size: 992 4748 BstXI CCAn_nnnn'nTGG Cuts at: 2173 2173 Size: 5740 BstYI r'GATC_y Cuts at: 1972 2125 2367 3070 3437 3822 3839 5291 5377 Size: 153 242 703 367 385 17 1452 86 Cuts at: 5377 5389 5735 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 703 385 367 346 242 153 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5188 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5001 Fragments arranged by size: 5001 683 56 BtsI GCAGTG_nn' Cuts at: 2889 3256 4050 4070 2889 Size: 367 794 20 4559 Fragments arranged by size: 4559 794 367 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5740 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5705 DraI TTT'AAA Cuts at: 3044 3044 Size: 5740 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4747 809 Size: 507 3431 1802 Fragments arranged by size: 3431 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2439 3187 4076 Size: 54 1425 50 275 18 609 748 889 Cuts at: 4076 5705 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 748 609 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3187 1537 Size: 275 1375 4090 Fragments arranged by size: 4090 1375 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2600 3003 3670 1353 Size: 1247 403 667 3423 Fragments arranged by size: 3423 1247 667 403 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4327 4701 4847 387 Size: 1064 138 2738 374 146 1280 Fragments arranged by size: 2738 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2874 3803 4400 5187 5455 1239 Size: 393 1242 929 597 787 268 1524 Fragments arranged by size: 1524 1242 929 787 597 393 268 EcoRI G'AATT_C Cuts at: 3049 3427 3049 Size: 378 5362 FspI TGC'GCA Cuts at: 4246 4246 Size: 5740 HaeII r_GCGC'y Cuts at: 1678 4887 1678 Size: 3209 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2043 2075 2110 2142 2451 Size: 32 135 32 471 32 35 32 309 Cuts at: 2451 2483 2980 3012 4381 4413 4455 4487 1373 Size: 32 497 32 1369 32 42 32 2626 Fragments arranged by size: 2626 1369 497 471 309 135 42 35 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2517 3084 3330 669 Size: 988 281 579 567 246 3079 Fragments arranged by size: 3079 988 579 567 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5740 HpaI GTT'AAC Cuts at: 3330 3330 Size: 5740 KpnI G_GTAC'C Cuts at: 3065 3065 Size: 5740 MfeI C'AATT_G Cuts at: 3339 3339 Size: 5740 MluI A'CGCG_T Cuts at: 5616 5616 Size: 5740 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2647 4854 5038 1305 Size: 1342 2207 184 2007 Fragments arranged by size: 2207 2007 1342 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2441 10 Size: 54 1425 343 609 3309 Fragments arranged by size: 3309 1425 609 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2240 3698 4057 4216 518 Size: 648 843 231 1458 359 159 2042 Fragments arranged by size: 2042 1458 843 648 359 231 159 MspA1I CmG'CkG Cuts at: 1606 2017 3156 3833 4981 5226 1606 Size: 411 1139 677 1148 245 2120 Fragments arranged by size: 2120 1148 1139 677 411 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5188 NdeI CA'TA_TG Cuts at: 387 387 Size: 5740 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5740 NotI GC'GGCC_GC Cuts at: 3187 3187 Size: 5740 NspI r_CATG'y Cuts at: 1132 1229 3098 4643 1132 Size: 97 1869 1545 2229 Fragments arranged by size: 2229 1869 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5740 PciI A'CATG_T Cuts at: 4639 4639 Size: 5740 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5740 PmeI GTTT'AAAC Cuts at: 3044 3044 Size: 5740 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3809 3841 5322 5354 3809 Size: 32 1481 32 4195 Fragments arranged by size: 4195 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5740 PsiI TTA'TAA Cuts at: 3310 3310 Size: 5740 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2891 2923 2891 Size: 32 5708 PstI C_TGCA'G Cuts at: 830 2139 2884 3092 830 Size: 1309 745 208 3478 Fragments arranged by size: 3478 1309 745 208 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5740 PvuII CAG'CTG Cuts at: 2017 2017 Size: 5740 SalI G'TCGA_C Cuts at: 1936 3082 1936 Size: 1146 4594 SapI GCTCTTCn'nnn_ Cuts at: 2600 2600 Size: 5740 SbfI CC_TGCA'GG Cuts at: 3092 3092 Size: 5740 ScaI AGT'ACT Cuts at: 3988 5700 3988 Size: 1712 4028 SfcI C'TryA_G Cuts at: 826 1045 2135 2880 2935 3088 4223 4904 5095 Size: 219 1090 745 55 153 1135 681 191 Cuts at: 5095 826 Size: 1471 Fragments arranged by size: 1471 1135 1090 745 681 219 191 153 55 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5740 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5740 SmaI CCC'GGG Cuts at: 1872 3067 1872 Size: 1195 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2968 3844 4745 5007 820 Size: 197 926 1025 876 901 262 1553 Fragments arranged by size: 1553 1025 926 901 876 262 197 SnaBI TAC'GTA Cuts at: 493 3531 493 Size: 3038 2702 SpeI A'CTAG_T Cuts at: 152 2519 152 Size: 2367 3373 SphI G_CATG'C Cuts at: 1132 3098 1132 Size: 1966 3774 SspI AAT'ATT Cuts at: 5 52 3664 5 Size: 47 3612 2081 Fragments arranged by size: 3612 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5740 StyI C'CwwG_G Cuts at: 513 1065 2870 3179 513 Size: 552 1805 309 3074 Fragments arranged by size: 3074 1805 552 309 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2378 3767 3784 3937 4122 4567 325 Size: 1255 798 1389 17 153 185 445 1498 Fragments arranged by size: 1498 1389 1255 798 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2169 2244 2841 Size: 274 80 33 51 1443 192 75 597 Cuts at: 2841 3986 5698 96 Size: 1145 1712 138 Fragments arranged by size: 1712 1443 1145 597 274 192 138 80 75 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5700 5732 5700 Size: 32 5708 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5740 XbaI T'CTAG_A Cuts at: 3034 3076 3034 Size: 42 5698 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5740 XmaI C'CCGG_G Cuts at: 1870 3065 1870 Size: 1195 4545 XmnI GAAnn'nnTTC Cuts at: 3869 3869 Size: 5740 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3548 276 Size: 53 83 186 936 2014 2468 Fragments arranged by size: 2468 2014 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI