Restriction map for
pFN21AE6004
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# NO CUT ENZYMES ( 146 enzymes) #
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AatII Acc65I AccI AccII AclI
AcsI AflII AgeI AhdI Alw44I
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AviII AvrII BalI
BamHI BbeI BbsI BcgI BclI
BfaI BfrI BglII BlnI BpmI
BpuAI BsaI BsiEI BsiWI BsmI
BsoBI BspDI BspHI BspLU11I BsrBI
BsrDI BsrFI BsrI BssHII BssSI
Bst1107I BstAPI BstBI BstUI BstXI
BstYI BstZ17I Cfr10I ClaI CpoI
DraI DraII DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO109I EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI KasI KpnI KspI MaeI
MfeI MflI MluI MluNI MscI
MseI MspA1I MunI MvnI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmeI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI Sse8387I SwaI TaqI
Tru9I Tsp509I Tth111I TthHB8I Van91I
XbaI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccIII 882 TCCGGA T^CCGGA
AflIII 659 ACGTGT A^C[GA][CT]GT
AlwNI 382 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 602 CCTCAGG CC^T[ACGT]AGG
AspHI 457 GTGCTC G[AT]GC[AT]^C
AvaII 345 GGTCC G^G[AT]CC
BbrPI 658 CACGTG CAC^GTG
BbvCI 328 GCTGAGG CCTCAGC(-5/-2)
BbvI 292 GCAGC GCAGC(8/12)
BciVI 393 GTATCC GTATCC(6/5)
BcnI 158 CCCGG CC^[GC]GG
BglI 113 GCCTTCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 658 CACGTG [CT]AC^GT[GA]
BsaBI 7 GATGTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 882 TCCGGA [AT]^CCGG[AT]
BseAI 882 TCCGGA T^CCGGA
BsiHKAI 457 GTGCTC G[AT]GC[AT]^C
BsmAI 408 GAGAC GTCTC(1/5)
BsmBI 408 GAGACG CGTCTC(1/5)
BsmFI 218 GTCCC GGGAC(10/14)
Bsp1407I 773 TGTACA T^GTACA
BspEI 882 TCCGGA T^CCGGA
BspMI 718 ACCTGC ACCTGC(4/8)
BsrGI 773 TGTACA T^GTACA
BstEII 225 GGTCACC G^GT[ACGT]ACC
BstPI 225 GGTCACC G^GT[ACGT]ACC
Bsu36I 602 CCTCAGG CC^T[ACGT]AGG
DsaI 221 CCATGG C^C[GA][CT]GG
EaeI 654 CGGCCA [CT]^GGCC[GA]
Eco81I 602 CCTCAGG CC^T[ACGT]AGG
EcoO65I 225 GGTCACC G^GT[ACGT]ACC
EcoT14I 221 CCATGG C^C[AT][AT]GG
FauI 258 GCGGG CCCGC(4/6)
HaeII 591 GGCGCT [GA]GCGC^[CT]
MamI 7 GATGTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 882 TCCGGA T^CCGGA
NciI 158 CCCGG CC^[GC]GG
NcoI 221 CCATGG C^CATGG
NspI 680 GCATGC [GA]CATG^[CT]
PmaCI 658 CACGTG CAC^GTG
PmlI 658 CACGTG CAC^GTG
PstI 388 CTGCAG CTGCA^G
SfaNI 244 GCATC GCATC(5/9)
SphI 680 GCATGC GCATG^C
SspBI 773 TGTACA T^GTACA
SspI 300 AATATT AAT^ATT
StuI 111 AGGCCT AGG^CCT
StyI 221 CCATGG C^C[AT][AT]GG
TseI 292 GCAGC G^C[AT]GC
TspRI 314 CACTG CA[GC]TG[ACGT][ACGT]^
XcmI 230 CCATCCCCAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 21 enzymes) #
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AciI 761 CCGC CCGC(-3/-1)
258 GCGG
AcyI 334 GGCGTC G[GA]^CG[CT]C
410 GACGCC
BanII 435 GGGCTC G[GA]GC[CT]^C
745 GGGCTC
BlpI 186 GCTCAGC GC^T[ACGT]AGC
912 GCTTAGC
Bpu1102I 186 GCTCAGC GC^T[ACGT]AGC
912 GCTTAGC
BsaHI 334 GGCGTC G[GA]^CG[CT]C
410 GACGCC
BsgI 290 GTGCAG GTGCAG(16/14)
566 GTGCAG
CelII 186 GCTCAGC GC^T[ACGT]AGC
912 GCTTAGC
Cfr13I 45 GGGCC G^G[ACGT]CC
345 GGTCC
Fnu4HI 292 GCAGC GC^[ACGT]GC
760 GCCGC
HapII 159 CCGG C^CGG
883 CCGG
HgaI 410 GACGC GACGC(5/10)
335 GCGTC
Hin1I 334 GGCGTC G[GA]^CG[CT]C
410 GACGCC
HpaII 159 CCGG C^CGG
883 CCGG
ItaI 292 GCAGC GC^[ACGT]GC
760 GCCGC
MaeII 659 ACGT A^CGT
815 ACGT
MspI 159 CCGG C^CGG
883 CCGG
MwoI 113 GCCTTCTGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
186 GCTCAGCCAGC
PleI 154 GACTC GAGTC(4/5)
926 GACTC
Sau96I 45 GGGCC G^G[ACGT]CC
345 GGTCC
TfiI 12 GAATC G^A[AT]TC
879 GAATC
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# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AfaI AluI AlwI BanI BmyI
BsaJI BseRI BsiYI BslI Bsp1286I
BssKI BstF5I BstNI Cac8I CfoI
DdeI DpnII EarI EcoRII FokI
HaeIII HhaI HinP1I HinfI HphI
Ksp632I MaeIII MboI MboII MnlI
MslI MvaI NdeII NlaIII NlaIV
RsaI Sau3AI ScrFI SfcI Tsp45I