(Circular) (Six-base) MAPSORT of: pFN21AE6004.seq Check: 1714 from: 1 to: 5647 pFN21AE6004 5647 bp With 182 enzymes: SgfI * December 8, 2014 13:49 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2732 2732 Size: 5647 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3457 278 Size: 53 83 186 936 1921 2468 Fragments arranged by size: 2468 1921 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2990 1937 Size: 1053 4594 Acc65I G'GTAC_C Cuts at: 2968 2968 Size: 5647 AclI AA'CG_TT Cuts at: 1563 3774 4147 1563 Size: 2211 373 3063 Fragments arranged by size: 3063 2211 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2041 2579 3710 5094 5362 1632 Size: 409 538 1131 1384 268 1917 Fragments arranged by size: 1917 1384 1131 538 409 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5450 AflIII A'CryG_T Cuts at: 1203 2664 4546 5523 1203 Size: 1461 1882 977 1327 Fragments arranged by size: 1882 1461 1327 977 AgeI A'CCGG_T Cuts at: 4478 4478 Size: 5647 AhdI GACnn_n'nnGTC Cuts at: 4376 4376 Size: 5647 AleI CACnn'nnGTG Cuts at: 1166 2744 1166 Size: 1578 4069 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5647 AlwNI CAG_nnn'CTG Cuts at: 2392 3390 4962 2392 Size: 998 1572 3077 Fragments arranged by size: 3077 1572 998 ApaLI G'TGCA_C Cuts at: 3704 4860 3704 Size: 1156 4491 ApoI r'AATT_y Cuts at: 1493 2956 3182 3334 5443 1493 Size: 1463 226 152 2109 1697 Fragments arranged by size: 2109 1697 1463 226 152 AseI AT'TA_AT Cuts at: 160 1032 4201 160 Size: 872 3169 1606 Fragments arranged by size: 3169 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5647 AvaI C'yCGr_G Cuts at: 1870 1943 2972 1870 Size: 73 1029 4545 Fragments arranged by size: 4545 1029 73 BamHI G'GATC_C Cuts at: 2977 3344 2977 Size: 367 5280 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2273 2545 2762 2785 2968 Size: 325 217 627 486 272 217 23 183 Cuts at: 2968 4423 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 486 325 272 217 217 183 23 BanII G_rGCy'C Cuts at: 1391 2444 2754 2966 1391 Size: 1053 310 212 4072 Fragments arranged by size: 4072 1053 310 212 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4676 BbvCI CC'TCA_GC Cuts at: 2334 2334 Size: 5647 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3838 3872 1903 Size: 34 1901 34 3678 Fragments arranged by size: 3678 1901 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2409 3539 4749 2409 Size: 1130 1210 3307 Fragments arranged by size: 3307 1210 1130 BclI T'GATC_A Cuts at: 1548 1548 Size: 5647 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2124 4258 136 Size: 107 122 71 631 605 452 2134 1525 Fragments arranged by size: 2134 1525 631 605 452 122 107 71 BglII A'GATC_T Cuts at: 5642 5642 Size: 5647 BlpI GC'TnA_GC Cuts at: 2192 2918 3064 2192 Size: 726 146 4775 Fragments arranged by size: 4775 726 146 Bme1580I G_kGCm'C Cuts at: 1790 3708 4864 1790 Size: 1918 1156 2573 Fragments arranged by size: 2573 1918 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5647 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3378 4336 442 Size: 416 53 83 399 24 1961 958 1753 Fragments arranged by size: 1961 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5647 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2948 2948 Size: 5647 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4307 1239 Size: 3068 2579 Bpu10I CC'TnA_GC Cuts at: 2334 2334 Size: 5647 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3772 4637 4935 3772 Size: 865 298 4484 Fragments arranged by size: 4484 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4310 882 Size: 617 2811 2219 Fragments arranged by size: 2811 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2665 3438 493 Size: 711 1461 773 2702 Fragments arranged by size: 2702 1461 773 711 BsaBI GATnn'nnATC Cuts at: 2016 5641 2016 Size: 3625 2022 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2340 2416 3454 3836 Size: 53 83 186 936 807 76 1038 382 Cuts at: 3836 4471 275 Size: 635 1451 Fragments arranged by size: 1451 1038 936 807 635 382 186 83 76 53 BsaWI w'CCGG_w Cuts at: 2887 4080 4478 4752 4899 5310 2887 Size: 1193 398 274 147 411 3224 Fragments arranged by size: 3224 1193 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2064 2094 2498 2528 1178 Size: 30 165 30 661 30 404 30 4297 Fragments arranged by size: 4297 661 404 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2072 2434 2711 2719 1186 Size: 886 362 277 8 4114 Fragments arranged by size: 4114 886 362 277 8 BseYI C'CCAG_C Cuts at: 4850 5346 4850 Size: 496 5151 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2316 2592 1163 Size: 573 580 276 4218 Fragments arranged by size: 4218 580 573 276 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3097 3858 4886 1540 Size: 275 186 1096 761 1028 2301 Fragments arranged by size: 2301 1096 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2466 3708 3793 4864 2466 Size: 1242 85 1071 3249 Fragments arranged by size: 3249 1242 1071 85 BsmI GAATG_Cn' Cuts at: 3158 3251 3158 Size: 93 5554 BsmBI CGTCTCn'nnnn_ Cuts at: 2407 2407 Size: 5647 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2444 2466 2754 2966 3708 3793 4864 Size: 399 654 22 288 212 742 85 1071 Cuts at: 4864 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 654 399 288 212 85 22 BspEI T'CCGG_A Cuts at: 2887 5310 2887 Size: 2423 3224 BspHI T'CATG_A Cuts at: 3536 5266 3536 Size: 1730 3917 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2732 3002 844 Size: 1888 270 3489 Fragments arranged by size: 3489 1888 270 BsrBI CCG'CTC Cuts at: 1394 1823 3534 1394 Size: 429 1711 3507 Fragments arranged by size: 3507 1711 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4142 4316 65 Size: 1183 2894 174 1396 Fragments arranged by size: 2894 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4291 4478 1700 Size: 110 2481 187 2869 Fragments arranged by size: 2869 2481 187 110 BsrGI T'GTAC_A Cuts at: 96 2778 96 Size: 2682 2965 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5647 BssSI C'ACGA_G Cuts at: 3707 4719 3707 Size: 1012 4635 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5647 BstBI TT'CG_AA Cuts at: 4451 4451 Size: 5647 BstEII G'GTnAC_C Cuts at: 1184 2230 1184 Size: 1046 4601 BstYI r'GATC_y Cuts at: 1972 2977 3344 3729 3746 5198 5284 5296 5642 Size: 1005 367 385 17 1452 86 12 346 Cuts at: 5642 1972 Size: 1977 Fragments arranged by size: 1977 1452 1005 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2608 2608 Size: 5647 BtgI C'CryG_G Cuts at: 513 1065 2004 2226 513 Size: 552 939 222 3934 Fragments arranged by size: 3934 939 552 222 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2629 487 Size: 683 56 1403 3505 Fragments arranged by size: 3505 1403 683 56 BtsI GCAGTG_nn' Cuts at: 3163 3957 3977 3163 Size: 794 20 4833 Fragments arranged by size: 4833 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5647 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5612 DraI TTT'AAA Cuts at: 2951 2951 Size: 5647 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4654 809 Size: 507 3338 1802 Fragments arranged by size: 3338 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2659 3094 3983 Size: 54 1425 50 275 18 829 435 889 Cuts at: 3983 5612 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 829 435 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3094 1537 Size: 275 1282 4090 Fragments arranged by size: 4090 1282 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2060 2090 2147 2837 3577 1353 Size: 707 30 57 690 740 3423 Fragments arranged by size: 3423 740 707 690 57 30 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4234 4608 4754 387 Size: 1064 138 2645 374 146 1280 Fragments arranged by size: 2645 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2041 2579 3710 4307 5094 5362 1239 Size: 393 409 538 1131 597 787 268 1524 Fragments arranged by size: 1524 1131 787 597 538 409 393 268 EcoRI G'AATT_C Cuts at: 2956 3334 2956 Size: 378 5269 FspI TGC'GCA Cuts at: 4153 4153 Size: 5647 HaeII r_GCGC'y Cuts at: 1678 2600 4794 1678 Size: 922 2194 2531 Fragments arranged by size: 2531 2194 922 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4288 4320 4362 4394 1373 Size: 32 135 32 2716 32 42 32 2626 Fragments arranged by size: 2716 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2991 3237 669 Size: 988 281 1053 246 3079 Fragments arranged by size: 3079 1053 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5647 HpaI GTT'AAC Cuts at: 3237 3237 Size: 5647 KpnI G_GTAC'C Cuts at: 2972 2972 Size: 5647 MfeI C'AATT_G Cuts at: 3246 3246 Size: 5647 MluI A'CGCG_T Cuts at: 5523 5523 Size: 5647 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4761 4945 1305 Size: 3456 184 2007 Fragments arranged by size: 3456 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3825 Fragments arranged by size: 3825 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2045 2321 2744 2780 3605 3964 4123 Size: 648 879 276 423 36 825 359 159 Cuts at: 4123 518 Size: 2042 Fragments arranged by size: 2042 879 825 648 423 359 276 159 36 MspA1I CmG'CkG Cuts at: 1606 3063 3740 4888 5133 1606 Size: 1457 677 1148 245 2120 Fragments arranged by size: 2120 1457 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2226 513 Size: 552 939 222 3934 Fragments arranged by size: 3934 939 552 222 NdeI CA'TA_TG Cuts at: 387 387 Size: 5647 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5647 NotI GC'GGCC_GC Cuts at: 3094 3094 Size: 5647 NspI r_CATG'y Cuts at: 1132 1229 2689 3005 4550 1132 Size: 97 1460 316 1545 2229 Fragments arranged by size: 2229 1545 1460 316 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5647 PciI A'CATG_T Cuts at: 4546 4546 Size: 5647 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5647 PmeI GTTT'AAAC Cuts at: 2951 2951 Size: 5647 PmlI CAC'GTG Cuts at: 2665 2665 Size: 5647 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3716 3748 5229 5261 3716 Size: 32 1481 32 4102 Fragments arranged by size: 4102 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5647 PsiI TTA'TAA Cuts at: 3217 3217 Size: 5647 PstI C_TGCA'G Cuts at: 830 2397 2999 830 Size: 1567 602 3478 Fragments arranged by size: 3478 1567 602 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5647 SalI G'TCGA_C Cuts at: 1936 2989 1936 Size: 1053 4594 SbfI CC_TGCA'GG Cuts at: 2999 2999 Size: 5647 ScaI AGT'ACT Cuts at: 3895 5607 3895 Size: 1712 3935 SfcI C'TryA_G Cuts at: 826 1045 2393 2836 2872 2995 4130 4811 5002 Size: 219 1348 443 36 123 1135 681 191 Cuts at: 5002 826 Size: 1471 Fragments arranged by size: 1471 1348 1135 681 443 219 191 123 36 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5647 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5647 SmaI CCC'GGG Cuts at: 1872 2974 1872 Size: 1102 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3751 4652 4914 820 Size: 197 926 1808 901 262 1553 Fragments arranged by size: 1808 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3438 493 Size: 2945 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5647 SphI G_CATG'C Cuts at: 1132 2689 3005 1132 Size: 1557 316 3774 Fragments arranged by size: 3774 1557 316 SspI AAT'ATT Cuts at: 5 52 2307 3571 5 Size: 47 2255 1264 2081 Fragments arranged by size: 2255 2081 1264 47 StuI AGG'CCT Cuts at: 1517 2118 1517 Size: 601 5046 StyI C'CwwG_G Cuts at: 513 1065 2004 2226 3086 513 Size: 552 939 222 860 3074 Fragments arranged by size: 3074 939 860 552 222 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2191 2315 2440 3674 3691 3844 4029 Size: 1255 611 124 125 1234 17 153 185 Cuts at: 4029 4474 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1234 611 445 185 153 125 124 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2181 2404 2778 Size: 274 80 33 51 1443 204 223 374 Cuts at: 2778 3893 5605 96 Size: 1115 1712 138 Fragments arranged by size: 1712 1443 1115 374 274 223 204 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2064 2096 5607 5639 2064 Size: 32 3511 32 2072 Fragments arranged by size: 3511 2072 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5647 XbaI T'CTAG_A Cuts at: 2983 2983 Size: 5647 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2242 2242 Size: 5647 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5647 XmaI C'CCGG_G Cuts at: 1870 2972 1870 Size: 1102 4545 XmnI GAAnn'nnTTC Cuts at: 3776 3776 Size: 5647 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3455 276 Size: 53 83 186 936 1921 2468 Fragments arranged by size: 2468 1921 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BstXI BstZ17I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI