################################## # NO CUT ENZYMES ( 139 enzymes) # ################################## Acc65I AccI AccII AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BalI BamHI BbrPI BcgI BciVI BclI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaWI BseAI BsiEI BsiHKAI BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BssHII Bst1107I BstBI BstEII BstPI BstUI BstZ17I CelII Cfr10I ClaI CpoI DraI DrdI DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV EcoT14I FbaI FseI FspI HincII HindII HindIII HpaI KpnI Ksp632I KspI MamI MfeI MluI MluNI MroI MscI MseI MunI MvnI NaeI NcoI NdeI NgoMIV NheI NotI NruI NspI NspV PacI PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StyI SwaI TfiI Tru9I Tth111I XbaI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AatII 274 GACGTC GACGT^C AlwI 486 GGATC GGATC(4/5) AocI 298 CCTCAGG CC^T[ACGT]AGG ApaI 120 GGGCCC GGGCC^C BanII 120 GGGCCC G[GA]GC[CT]^C BbeI 226 GGCGCC GGCGC^C BbsI 818 GAAGAC GAAGAC(2/6) BcnI 207 CCCGG CC^[GC]GG BfaI 562 CTAG C^TAG BglI 196 GCCCACATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpmI 667 CTCCAG CTGGAG(16/14) BpuAI 818 GAAGAC GAAGAC(2/6) BsiWI 702 CGTACG C^GTACG BsmBI 921 GAGACG CGTCTC(1/5) BsmI 575 GAATGC GAATGC(1/-1) Bsp1407I 768 TGTACA T^GTACA BsrGI 768 TGTACA T^GTACA BssSI 25 CTCGTG CACGAG(-5/-1) BstAPI 983 GCAAGAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstYI 486 GGATCT [GA]^GATC[CT] Bsu36I 298 CCTCAGG CC^T[ACGT]AGG DpnII 487 GATC ^GATC DraIII 166 CACCCCGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 435 CGGCCA [CT]^GGCC[GA] Eco81I 298 CCTCAGG CC^T[ACGT]AGG EcoT22I 937 ATGCAT ATGCA^T HapII 208 CCGG C^CGG HgaI 923 GACGC GACGC(5/10) HpaII 208 CCGG C^CGG KasI 226 GGCGCC G^GCGCC MaeI 562 CTAG C^TAG MboI 487 GATC ^GATC MflI 486 GGATCT [GA]^GATC[CT] MslI 606 CACACAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 208 CCGG C^CGG NarI 226 GGCGCC GG^CGCC NciI 207 CCCGG CC^[GC]GG NdeII 487 GATC ^GATC NsiI 937 ATGCAT ATGCA^T PaeR7I 733 CTCGAG C^TCGAG PflMI 34 CCAGTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 282 AGGTCCC [GA]G^G[AT]CC[CT] Sau3AI 487 GATC ^GATC SfoI 226 GGCGCC GGC^GCC SspBI 768 TGTACA T^GTACA StuI 186 AGGCCT AGG^CCT Tsp509I 885 AATT ^AATT Van91I 34 CCAGTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 995 CCATCTGCCGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 733 CTCGAG C^TCGAG XhoII 486 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AfaI 703 GTAC GT^AC 769 GTAC AvaI 733 CTCGAG C^[CT]CG[GA]G 758 CTCGGG BanI 61 GGTGCC G^G[CT][GA]CC 226 GGCGCC BsaI 77 GGTCTC GGTCTC(1/5) 363 GGTCTC BsmFI 672 GGGAC GGGAC(10/14) 284 GTCCC BsoBI 733 CTCGAG C^[CT]CG[GA]G 758 CTCGGG BstXI 458 CCAGGTATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 605 CCACACAGATGG CfoI 194 GCGC GCG^C 227 GCGC EcoNI 68 CCTTTCAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 294 CCTACCTCAGG HaeII 193 AGCGCC [GA]GCGC^[CT] 226 GGCGCC HhaI 194 GCGC GCG^C 227 GCGC HinP1I 194 GCGC G^CGC 227 GCGC HphI 791 TCACC GGTGA(8/7) 948 TCACC MaeII 14 ACGT A^CGT 275 ACGT MaeIII 277 GTCAC ^GT[ACGT]AC 754 GTGAC RsaI 703 GTAC GT^AC 769 GTAC SmlI 507 CTTGAG C^T[CT][GA]AG 733 CTCGAG Tsp45I 277 GTCAC ^GT[GC]AC 754 GTGAC ######################################### # 3 OR MORE CUT ENZYMES ( 50 enzymes) # ######################################### AciI AcyI AluI AlwNI AvaII BbvCI BbvI BmyI BsaHI BsaJI BseRI BsgI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DraII EcoO109I EcoRII FauI Fnu4HI FokI HaeIII Hin1I HinfI ItaI MboII MnlI MspA1I MvaI MwoI NlaIII NlaIV PleI PstI Sau96I ScrFI SfaNI SfcI TaqI TseI TspRI TthHB8I