Restriction map for
pFN21AE4065
(Linear) (Six-base) MAPSORT of: KIEE4065.seq Check: 6222 from: 1 to: 1038
KIEE4065.orf
With 182 enzymes: SgfI *
March 22, 2012 17:18 ..
AatII G_ACGT'C
Cuts at: 0 278 1038
Size: 278 760
AlwNI CAG_nnn'CTG
Cuts at: 0 243 306 339 1038
Size: 243 63 33 699
Fragments arranged by size:
699 243 63 33
ApaI G_GGCC'C
Cuts at: 0 124 1038
Size: 124 914
AvaI C'yCGr_G
Cuts at: 0 733 758 1038
Size: 733 25 280
Fragments arranged by size:
733 280 25
BanI G'GyrC_C
Cuts at: 0 61 226 1038
Size: 61 165 812
Fragments arranged by size:
812 165 61
BanII G_rGCy'C
Cuts at: 0 124 1038
Size: 124 914
BbeI G_GCGC'C
Cuts at: 0 230 1038
Size: 230 808
BbsI GAAGACnn'nnnn_
Cuts at: 0 825 1038
Size: 825 213
BbvCI CC'TCA_GC
Cuts at: 0 190 452 470 542 647 1038
Size: 190 262 18 72 105 391
Fragments arranged by size:
391 262 190 105 72 18
BfrBI ATG'CAT
Cuts at: 0 939 1038
Size: 939 99
BglI GCCn_nnn'nGGC
Cuts at: 0 202 1038
Size: 202 836
Bme1580I G_kGCm'C
Cuts at: 0 66 124 478 1038
Size: 66 58 354 560
Fragments arranged by size:
560 354 66 58
BmgBI CAC'GTC
Cuts at: 0 15 1038
Size: 15 1023
BmrI ACTGGGnnnn_n'
Cuts at: 0 492 1033 1038
Size: 492 541 5
Fragments arranged by size:
541 492 5
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 533 1038
Size: 533 505
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 652 1038
Size: 652 386
Bpu10I CC'TnA_GC
Cuts at: 0 190 452 470 542 647 1038
Size: 190 262 18 72 105 391
Fragments arranged by size:
391 262 190 105 72 18
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 528 1038
Size: 528 510
BsaI GGTCTCn'nnnn_
Cuts at: 0 83 369 1038
Size: 83 286 669
Fragments arranged by size:
669 286 83
BsaHI Gr'CG_yC
Cuts at: 0 227 275 924 1038
Size: 227 48 649 114
Fragments arranged by size:
649 227 114 48
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 82 112 586 616 902 932 1038
Size: 82 30 474 30 286 30 106
Fragments arranged by size:
474 286 106 82 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 560 588 591 733 871 1038
Size: 560 28 3 142 138 167
Fragments arranged by size:
560 167 142 138 28 3
BseYI C'CCAG_C
Cuts at: 0 347 421 682 1038
Size: 347 74 261 356
Fragments arranged by size:
356 347 261 74
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 193 217 292 1038
Size: 193 24 75 746
Fragments arranged by size:
746 193 75 24
BsiWI C'GTAC_G
Cuts at: 0 702 1038
Size: 702 336
BsmI GAATG_Cn'
Cuts at: 0 581 1038
Size: 581 457
BsmBI CGTCTCn'nnnn_
Cuts at: 0 915 1038
Size: 915 123
Bsp1286I G_dGCh'C
Cuts at: 0 66 124 478 1038
Size: 66 58 354 560
Fragments arranged by size:
560 354 66 58
BsrGI T'GTAC_A
Cuts at: 0 768 1038
Size: 768 270
BssSI C'ACGA_G
Cuts at: 0 25 1038
Size: 25 1013
BstAPI GCAn_nnn'nTGC
Cuts at: 0 989 1038
Size: 989 49
BstXI CCAn_nnnn'nTGG
Cuts at: 0 465 612 1038
Size: 465 147 426
Fragments arranged by size:
465 426 147
BstYI r'GATC_y
Cuts at: 0 486 1038
Size: 486 552
Bsu36I CC'TnA_GG
Cuts at: 0 299 1038
Size: 299 739
DraIII CAC_nnn'GTG
Cuts at: 0 171 1038
Size: 171 867
EaeI y'GGCC_r
Cuts at: 0 435 1038
Size: 435 603
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 393 687 1038
Size: 393 294 351
Fragments arranged by size:
393 351 294
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 652 1038
Size: 652 386
EcoNI CCTnn'n_nnAGG
Cuts at: 0 72 298 1038
Size: 72 226 740
Fragments arranged by size:
740 226 72
EcoO109I rG'GnC_Cy
Cuts at: 0 121 283 303 1038
Size: 121 162 20 735
Fragments arranged by size:
735 162 121 20
HaeII r_GCGC'y
Cuts at: 0 197 230 1038
Size: 197 33 808
Fragments arranged by size:
808 197 33
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 379 411 1038
Size: 379 32 627
Fragments arranged by size:
627 379 32
KasI G'GCGC_C
Cuts at: 0 226 1038
Size: 226 812
MslI CAynn'nnrTG
Cuts at: 0 610 1038
Size: 610 428
MspA1I CmG'CkG
Cuts at: 0 159 224 252 1038
Size: 159 65 28 786
Fragments arranged by size:
786 159 65 28
NarI GG'CG_CC
Cuts at: 0 227 1038
Size: 227 811
NsiI A_TGCA'T
Cuts at: 0 941 1038
Size: 941 97
PflMI CCAn_nnn'nTGG
Cuts at: 0 40 1038
Size: 40 998
PfoI T'CCnGG_A
Cuts at: 0 269 338 1038
Size: 269 69 700
Fragments arranged by size:
700 269 69
PpuMI rG'GwC_Cy
Cuts at: 0 283 1038
Size: 283 755
PspOMI G'GGCC_C
Cuts at: 0 120 1038
Size: 120 918
PspXI vC'TCGA_Gb
Cuts at: 0 733 1038
Size: 733 305
PstI C_TGCA'G
Cuts at: 0 266 329 446 731 1038
Size: 266 63 117 285 307
Fragments arranged by size:
307 285 266 117 63
SfcI C'TryA_G
Cuts at: 0 262 325 442 727 1038
Size: 262 63 117 285 311
Fragments arranged by size:
311 285 262 117 63
SfoI GGC'GCC
Cuts at: 0 228 1038
Size: 228 810
SmlI C'TyrA_G
Cuts at: 0 507 733 1038
Size: 507 226 305
Fragments arranged by size:
507 305 226
StuI AGG'CCT
Cuts at: 0 188 1038
Size: 188 850
TatI w'GTAC_w
Cuts at: 0 768 1038
Size: 768 270
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 82 114 902 934 1038
Size: 82 32 788 32 104
Fragments arranged by size:
788 104 82 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1002 1038
Size: 1002 36
XhoI C'TCGA_G
Cuts at: 0 733 1038
Size: 733 305
ZraI GAC'GTC
Cuts at: 0 276 1038
Size: 276 762
Enzymes that do cut:
AatII AlwNI ApaI AvaI BanI BanII BbeI BbsI
BbvCI BfrBI BglI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaHI BsaXI BseRI BseYI BsgI
BsiWI BsmI BsmBI Bsp1286I BsrGI BssSI BstAPI BstXI
BstYI Bsu36I DraIII EaeI EciI Eco57MI EcoNI EcoO109I
HaeII Hin4I KasI MslI MspA1I NarI NsiI PflMI
PfoI PpuMI PspOMI PspXI PstI SfcI SfoI SmlI
StuI TatI TstI XcmI XhoI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BcgI BciVI BclI
BglII BlpI BmtI BsaAI BsaBI BsaWI BsiEI BsiHKAI
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BstBI
BstEII BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EagI EarI EcoICRI EcoRI EcoRV FalI FseI
FspI FspAI HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI NaeI NcoI NdeI NgoMIV NheI NotI
NruI NspI PacI PasI PciI PmeI PmlI PpiI
PshAI PsiI PsrI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI StyI
SwaI TaqII Tth111I XbaI XmaI XmnI