Restriction map for
pFN21AE5309
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# NO CUT ENZYMES ( 148 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcyI AflII AgeI AhdI Alw44I
Aor51HI ApaLI AscI AseI AsnI
Asp718I AspEI AspHI AspI AviII
AvrII BalI BanI BbeI BbrPI
BbsI BcgI BciVI BclI BfrI
BglI BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaI BsgI
BsiEI BsiHKAI BsiWI BsmBI Bsp1407I
BspDI BspHI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I CelII CfoI Cfr10I ClaI
CpoI DraI DraIII DrdI EaeI
EagI Eam1105I EarI EclXI Eco47III
Eco52I EcoO65I EcoRI EcoT22I FauI
FbaI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HpaI HphI
KasI KpnI Ksp632I KspI MaeII
MamI MfeI MluI MluNI MscI
MslI MunI MvnI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmlI SnaBI SphI Sse8387I
SspBI SspI StuI SwaI Tth111I
Van91I XbaI XhoI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccIII 48 TCCGGA T^CCGGA
AciI 350 GCGG CCGC(-3/-1)
AflIII 460 ACATGT A^C[GA][CT]GT
AlwNI 289 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 187 GGGCCC GGGCC^C
Asp700I 139 GAATTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 191 CCCGGG C^[CT]CG[GA]G
BamHI 42 GGATCC G^GATCC
BanII 187 GGGCCC G[GA]GC[CT]^C
BbvCI 730 GCTGAGG CCTCAGC(-5/-2)
BmyI 187 GGGCCC G[AGT]GC[ACT]^C
BsaWI 48 TCCGGA [AT]^CCGG[AT]
BseAI 48 TCCGGA T^CCGGA
BseRI 310 GAGGAG GAGGAG(10/8)
BsmI 299 GCATTC GAATGC(1/-1)
BsoBI 191 CCCGGG C^[CT]CG[GA]G
Bsp1286I 187 GGGCCC G[AGT]GC[ACT]^C
BspEI 48 TCCGGA T^CCGGA
BspLU11I 460 ACATGT A^CATGT
BspMI 411 GCAGGT ACCTGC(4/8)
BsrI 282 ACTGG ACTGG(1/-1)
Cac8I 777 GCCAGC GC[ACGT]^[ACGT]GC
DsaI 445 CCATGG C^C[GA][CT]GG
EcoNI 369 CCTGTTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 718 GATATC GAT^ATC
EcoT14I 445 CCATGG C^C[AT][AT]GG
HaeIII 188 GGCC GG^CC
HincII 483 GTCAAC GT[CT]^[GA]AC
HindII 483 GTCAAC GT[CT]^[GA]AC
HindIII 665 AAGCTT A^AGCTT
MaeIII 364 GTGAC ^GT[ACGT]AC
MroI 48 TCCGGA T^CCGGA
MspA1I 517 CAGCTG C[AC]G^C[GT]G
NcoI 445 CCATGG C^CATGG
NspI 460 ACATGT [GA]CATG^[CT]
PpuMI 413 AGGTCCC [GA]G^G[AT]CC[CT]
PstI 316 CTGCAG CTGCA^G
PvuII 517 CAGCTG CAG^CTG
ScaI 121 AGTACT AGT^ACT
SfcI 316 CTGCAG C^T[GA][CT]AG
SmaI 191 CCCGGG CCC^GGG
SpeI 66 ACTAGT A^CTAGT
StyI 445 CCATGG C^C[AT][AT]GG
TaqI 636 TCGA T^CGA
TfiI 10 GAATC G^A[AT]TC
Tsp45I 364 GTGAC ^GT[GC]AC
TthHB8I 636 TCGA T^CGA
XmaCI 191 CCCGGG C^CCGGG
XmaI 191 CCCGGG C^CCGGG
XmnI 139 GAATTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 14 enzymes) #
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AcsI 103 AAATTT [GA]^AATT[CT]
163 AAATTT
AfaI 122 GTAC GT^AC
280 GTAC
AlwI 43 GATCC GGATC(4/5)
42 GGATC
ApoI 103 AAATTT [GA]^AATT[CT]
163 AAATTT
AvaII 414 GGTCC G^G[AT]CC
433 GGACC
BfaI 67 CTAG C^TAG
422 CTAG
BglII 263 AGATCT A^GATCT
320 AGATCT
BsmAI 178 GAGAC GTCTC(1/5)
464 GTCTC
DraII 187 GGGCCCC [GA]G^G[ACGT]CC[CT]
413 AGGTCCC
EcoO109I 187 GGGCCCC [GA]G^G[ACGT]CC[CT]
413 AGGTCCC
MaeI 67 CTAG C^TAG
422 CTAG
RsaI 122 GTAC GT^AC
280 GTAC
SfaNI 472 GCATC GCATC(5/9)
590 GCATC
XcmI 174 CCAGGAGACACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
436 CCAGCAGTGCCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AluI AocI BbvI BcnI BpmI
BsaJI BsiYI BslI BsmFI BssKI
BstF5I BstNI BstYI Bsu36I Cfr13I
DdeI DpnII Eco81I EcoRII Fnu4HI
FokI HapII HinfI HpaII ItaI
MboI MboII MflI MnlI MseI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PleI Sau3AI Sau96I
ScrFI Tru9I TseI Tsp509I TspRI
XhoII