(Circular) (Six-base) MAPSORT of: pFN21AE5309.seq Check: 938 from: 1 to: 5530 pFN21AE5309 5530 bp With 182 enzymes: SgfI * January 30, 2014 17:17 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3340 278 Size: 53 83 186 936 1804 2468 Fragments arranged by size: 2468 1804 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2873 1937 Size: 936 4594 Acc65I G'GTAC_C Cuts at: 2851 2851 Size: 5530 AclI AA'CG_TT Cuts at: 1563 3657 4030 1563 Size: 2094 373 3063 Fragments arranged by size: 3063 2094 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2661 3593 4977 5245 1632 Size: 1029 932 1384 268 1917 Fragments arranged by size: 1917 1384 1029 932 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5333 AflIII A'CryG_T Cuts at: 1203 2465 4429 5406 1203 Size: 1262 1964 977 1327 Fragments arranged by size: 1964 1327 1262 977 AgeI A'CCGG_T Cuts at: 4361 4361 Size: 5530 AhdI GACnn_n'nnGTC Cuts at: 4259 4259 Size: 5530 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5530 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5530 AlwNI CAG_nnn'CTG Cuts at: 2299 3273 4845 2299 Size: 974 1572 2984 Fragments arranged by size: 2984 1572 974 ApaI G_GGCC'C Cuts at: 2196 2196 Size: 5530 ApaLI G'TGCA_C Cuts at: 3587 4743 3587 Size: 1156 4374 ApoI r'AATT_y Cuts at: 1493 2108 2168 2839 3065 3217 5326 1493 Size: 615 60 671 226 152 2109 1697 Fragments arranged by size: 2109 1697 671 615 226 152 60 AseI AT'TA_AT Cuts at: 160 1032 4084 160 Size: 872 3052 1606 Fragments arranged by size: 3052 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5530 AvaI C'yCGr_G Cuts at: 1870 1943 2196 2855 1870 Size: 73 253 659 4545 Fragments arranged by size: 4545 659 253 73 BamHI G'GATC_C Cuts at: 2047 2860 3227 2047 Size: 813 367 4350 Fragments arranged by size: 4350 813 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2851 4306 618 Size: 325 217 627 1064 1455 1842 Fragments arranged by size: 1842 1455 1064 627 325 217 BanII G_rGCy'C Cuts at: 1391 2196 2849 1391 Size: 805 653 4072 Fragments arranged by size: 4072 805 653 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4559 BbvCI CC'TCA_GC Cuts at: 2736 2736 Size: 5530 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3721 3755 1903 Size: 34 1784 34 3678 Fragments arranged by size: 3678 1784 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3422 4632 3422 Size: 1210 4320 BclI T'GATC_A Cuts at: 1548 1548 Size: 5530 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4141 136 Size: 107 122 71 631 605 2469 1525 Fragments arranged by size: 2469 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2268 2325 5525 2268 Size: 57 3200 2273 Fragments arranged by size: 3200 2273 57 BlpI GC'TnA_GC Cuts at: 2947 2947 Size: 5530 Bme1580I G_kGCm'C Cuts at: 1790 2196 3591 4747 1790 Size: 406 1395 1156 2573 Fragments arranged by size: 2573 1395 1156 406 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5530 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3261 4219 442 Size: 416 53 83 399 24 1844 958 1753 Fragments arranged by size: 1844 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5530 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2614 2614 Size: 5530 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2040 2201 2309 2489 2688 4190 1239 Size: 801 161 108 180 199 1502 2579 Fragments arranged by size: 2579 1502 801 199 180 161 108 Bpu10I CC'TnA_GC Cuts at: 2300 2736 2300 Size: 436 5094 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3655 4520 4818 3655 Size: 865 298 4367 Fragments arranged by size: 4367 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4193 882 Size: 617 2694 2219 Fragments arranged by size: 2694 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3321 493 Size: 711 2117 2702 Fragments arranged by size: 2702 2117 711 BsaBI GATnn'nnATC Cuts at: 5524 5524 Size: 5530 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3337 3719 4354 275 Size: 53 83 186 936 1804 382 635 1451 Fragments arranged by size: 1804 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2053 3963 4361 4635 4782 5193 2053 Size: 1910 398 274 147 411 2390 Fragments arranged by size: 2390 1910 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2604 2634 1178 Size: 30 165 30 1201 30 4074 Fragments arranged by size: 4074 1201 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2330 1186 Size: 1144 4386 BseYI C'CCAG_C Cuts at: 2422 4733 5229 2422 Size: 2311 496 2723 Fragments arranged by size: 2723 2311 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4957 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2980 3741 4769 1540 Size: 275 186 979 761 1028 2301 Fragments arranged by size: 2301 1028 979 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3591 3676 4747 3591 Size: 85 1071 4374 Fragments arranged by size: 4374 1071 85 BsmI GAATG_Cn' Cuts at: 2304 3041 3134 2304 Size: 737 93 4700 Fragments arranged by size: 4700 737 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2196 2849 3591 3676 4747 1391 Size: 399 406 653 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 653 406 399 85 BspEI T'CCGG_A Cuts at: 2053 5193 2053 Size: 3140 2390 BspHI T'CATG_A Cuts at: 3419 5149 3419 Size: 1730 3800 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2407 2885 844 Size: 1563 478 3489 Fragments arranged by size: 3489 1563 478 BsrBI CCG'CTC Cuts at: 1394 1823 3417 1394 Size: 429 1594 3507 Fragments arranged by size: 3507 1594 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4025 4199 65 Size: 1183 2777 174 1396 Fragments arranged by size: 2777 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4174 4361 1700 Size: 110 2364 187 2869 Fragments arranged by size: 2869 2364 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5530 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5530 BssSI C'ACGA_G Cuts at: 3590 4602 3590 Size: 1012 4518 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5530 BstBI TT'CG_AA Cuts at: 4334 4334 Size: 5530 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5530 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2011 Size: 5530 BstYI r'GATC_y Cuts at: 1972 2047 2268 2325 2860 3227 3612 3629 5081 Size: 75 221 57 535 367 385 17 1452 Cuts at: 5081 5167 5179 5525 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 535 385 367 346 221 86 75 57 17 12 Bsu36I CC'TnA_GG Cuts at: 2043 2313 2625 2043 Size: 270 312 4948 Fragments arranged by size: 4948 312 270 BtgI C'CryG_G Cuts at: 513 1065 2004 2450 513 Size: 552 939 446 3593 Fragments arranged by size: 3593 939 552 446 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4791 Fragments arranged by size: 4791 683 56 BtsI GCAGTG_nn' Cuts at: 2451 3046 3840 3860 2451 Size: 595 794 20 4121 Fragments arranged by size: 4121 794 595 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5530 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5495 DraI TTT'AAA Cuts at: 2834 2834 Size: 5530 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4537 809 Size: 507 3221 1802 Fragments arranged by size: 3221 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2977 3866 5495 Size: 54 1425 50 275 18 1147 889 1629 Cuts at: 5495 8 Size: 43 Fragments arranged by size: 1629 1425 1147 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2977 1537 Size: 275 1165 4090 Fragments arranged by size: 4090 1165 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3460 1353 Size: 2107 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4117 4491 4637 387 Size: 1064 138 2528 374 146 1280 Fragments arranged by size: 2528 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2040 2201 2309 2489 2661 2688 3593 Size: 393 408 161 108 180 172 27 905 Cuts at: 3593 4190 4977 5245 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 905 787 597 408 393 268 180 172 161 108 27 EcoNI CCTnn'n_nnAGG Cuts at: 2378 2378 Size: 5530 EcoO109I rG'GnC_Cy Cuts at: 2193 2419 2193 Size: 226 5304 EcoRI G'AATT_C Cuts at: 2839 3217 2839 Size: 378 5152 EcoRV GAT'ATC Cuts at: 2725 2725 Size: 5530 FspI TGC'GCA Cuts at: 4036 4036 Size: 5530 HaeII r_GCGC'y Cuts at: 1678 4677 1678 Size: 2999 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4171 4203 4245 4277 1373 Size: 32 135 32 2599 32 42 32 2626 Fragments arranged by size: 2626 2599 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2490 2874 3120 669 Size: 988 281 552 384 246 3079 Fragments arranged by size: 3079 988 552 384 281 246 HindIII A'AGCT_T Cuts at: 748 2670 748 Size: 1922 3608 HpaI GTT'AAC Cuts at: 3120 3120 Size: 5530 KpnI G_GTAC'C Cuts at: 2855 2855 Size: 5530 MfeI C'AATT_G Cuts at: 3129 3129 Size: 5530 MluI A'CGCG_T Cuts at: 5406 5406 Size: 5530 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2358 4644 4828 1305 Size: 1053 2286 184 2007 Fragments arranged by size: 2286 2007 1053 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3708 Fragments arranged by size: 3708 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 3488 3847 4006 518 Size: 648 843 1479 359 159 2042 Fragments arranged by size: 2042 1479 843 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2524 2946 3623 4771 5016 1606 Size: 918 422 677 1148 245 2120 Fragments arranged by size: 2120 1148 918 677 422 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2450 513 Size: 552 939 446 3593 Fragments arranged by size: 3593 939 552 446 NdeI CA'TA_TG Cuts at: 387 387 Size: 5530 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5530 NotI GC'GGCC_GC Cuts at: 2977 2977 Size: 5530 NspI r_CATG'y Cuts at: 1132 1229 2469 2888 4433 1132 Size: 97 1240 419 1545 2229 Fragments arranged by size: 2229 1545 1240 419 97 PasI CC'CwG_GG Cuts at: 1348 2408 1348 Size: 1060 4470 PciI A'CATG_T Cuts at: 2465 4429 2465 Size: 1964 3566 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5530 PfoI T'CCnGG_A Cuts at: 2217 2531 2704 2217 Size: 314 173 5043 Fragments arranged by size: 5043 314 173 PmeI GTTT'AAAC Cuts at: 2834 2834 Size: 5530 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3599 3631 5112 5144 3599 Size: 32 1481 32 3985 Fragments arranged by size: 3985 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2419 2419 Size: 5530 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5530 PsiI TTA'TAA Cuts at: 3100 3100 Size: 5530 PspOMI G'GGCC_C Cuts at: 2192 2192 Size: 5530 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2268 2300 2268 Size: 32 5498 PstI C_TGCA'G Cuts at: 830 2325 2882 830 Size: 1495 557 3478 Fragments arranged by size: 3478 1495 557 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5530 PvuII CAG'CTG Cuts at: 2524 2524 Size: 5530 SalI G'TCGA_C Cuts at: 1936 2872 1936 Size: 936 4594 SbfI CC_TGCA'GG Cuts at: 2882 2882 Size: 5530 ScaI AGT'ACT Cuts at: 2128 3778 5490 2128 Size: 1650 1712 2168 Fragments arranged by size: 2168 1712 1650 SfcI C'TryA_G Cuts at: 826 1045 2321 2878 4013 4694 4885 826 Size: 219 1276 557 1135 681 191 1471 Fragments arranged by size: 1471 1276 1135 681 557 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5530 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5530 SmaI CCC'GGG Cuts at: 1872 2198 2857 1872 Size: 326 659 4545 Fragments arranged by size: 4545 659 326 SmlI C'TyrA_G Cuts at: 820 1017 1943 3634 4535 4797 820 Size: 197 926 1691 901 262 1553 Fragments arranged by size: 1691 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3321 493 Size: 2828 2702 SpeI A'CTAG_T Cuts at: 152 2071 152 Size: 1919 3611 SphI G_CATG'C Cuts at: 1132 2888 1132 Size: 1756 3774 SspI AAT'ATT Cuts at: 5 52 3454 5 Size: 47 3402 2081 Fragments arranged by size: 3402 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5530 StyI C'CwwG_G Cuts at: 513 1065 2004 2450 2969 513 Size: 552 939 446 519 3074 Fragments arranged by size: 3074 939 552 519 446 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3557 3574 3727 3912 4357 325 Size: 1255 1977 17 153 185 445 1498 Fragments arranged by size: 1977 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2126 3776 5488 Size: 274 80 33 51 1443 149 1650 1712 Cuts at: 5488 96 Size: 138 Fragments arranged by size: 1712 1650 1443 274 149 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2430 2462 2604 2636 5490 5522 2430 Size: 32 142 32 2854 32 2438 Fragments arranged by size: 2854 2438 142 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5530 XbaI T'CTAG_A Cuts at: 2866 2866 Size: 5530 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2186 2448 2186 Size: 262 5268 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5530 XmaI C'CCGG_G Cuts at: 1870 2196 2855 1870 Size: 326 659 4545 Fragments arranged by size: 4545 659 326 XmnI GAAnn'nnTTC Cuts at: 2148 3659 2148 Size: 1511 4019 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3338 276 Size: 53 83 186 936 1804 2468 Fragments arranged by size: 2468 1804 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I DraIII EcoICRI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI