Restriction map for
pFN21AE5309
(Linear) (Six-base) MAPSORT of: KIEE5309.seq Check: 9974 from: 1 to: 825
KIEE5309.orf
With 182 enzymes: SgfI *
January 29, 2014 11:55 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 656 825
Size: 656 169
AflIII A'CryG_T
Cuts at: 0 460 825
Size: 460 365
AlwNI CAG_nnn'CTG
Cuts at: 0 294 825
Size: 294 531
ApaI G_GGCC'C
Cuts at: 0 191 825
Size: 191 634
ApoI r'AATT_y
Cuts at: 0 103 163 825
Size: 103 60 662
Fragments arranged by size:
662 103 60
AvaI C'yCGr_G
Cuts at: 0 191 825
Size: 191 634
BamHI G'GATC_C
Cuts at: 0 42 825
Size: 42 783
BanII G_rGCy'C
Cuts at: 0 191 825
Size: 191 634
BbvCI CC'TCA_GC
Cuts at: 0 731 825
Size: 731 94
BglII A'GATC_T
Cuts at: 0 263 320 825
Size: 263 57 505
Fragments arranged by size:
505 263 57
Bme1580I G_kGCm'C
Cuts at: 0 191 825
Size: 191 634
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 609 825
Size: 609 216
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 35 196 304 484 683 825
Size: 35 161 108 180 199 142
Fragments arranged by size:
199 180 161 142 108 35
Bpu10I CC'TnA_GC
Cuts at: 0 295 731 825
Size: 295 436 94
Fragments arranged by size:
436 295 94
BsaWI w'CCGG_w
Cuts at: 0 48 825
Size: 48 777
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 599 629 825
Size: 599 30 196
Fragments arranged by size:
599 196 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 325 825
Size: 325 500
BseYI C'CCAG_C
Cuts at: 0 417 825
Size: 417 408
BsmI GAATG_Cn'
Cuts at: 0 299 825
Size: 299 526
Bsp1286I G_dGCh'C
Cuts at: 0 191 825
Size: 191 634
BspEI T'CCGG_A
Cuts at: 0 48 825
Size: 48 777
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 402 825
Size: 402 423
BstYI r'GATC_y
Cuts at: 0 42 263 320 825
Size: 42 221 57 505
Fragments arranged by size:
505 221 57 42
Bsu36I CC'TnA_GG
Cuts at: 0 38 308 620 825
Size: 38 270 312 205
Fragments arranged by size:
312 270 205 38
BtgI C'CryG_G
Cuts at: 0 445 825
Size: 445 380
BtsI GCAGTG_nn'
Cuts at: 0 446 825
Size: 446 379
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 35 196 304 484 656 683 825
Size: 35 161 108 180 172 27 142
Fragments arranged by size:
180 172 161 142 108 35 27
EcoNI CCTnn'n_nnAGG
Cuts at: 0 373 825
Size: 373 452
EcoO109I rG'GnC_Cy
Cuts at: 0 188 414 825
Size: 188 226 411
Fragments arranged by size:
411 226 188
EcoRV GAT'ATC
Cuts at: 0 720 825
Size: 720 105
HincII GTy'rAC
Cuts at: 0 485 825
Size: 485 340
HindIII A'AGCT_T
Cuts at: 0 665 825
Size: 665 160
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 353 825
Size: 353 472
MspA1I CmG'CkG
Cuts at: 0 519 825
Size: 519 306
NcoI C'CATG_G
Cuts at: 0 445 825
Size: 445 380
NspI r_CATG'y
Cuts at: 0 464 825
Size: 464 361
PasI CC'CwG_GG
Cuts at: 0 403 825
Size: 403 422
PciI A'CATG_T
Cuts at: 0 460 825
Size: 460 365
PfoI T'CCnGG_A
Cuts at: 0 212 526 699 825
Size: 212 314 173 126
Fragments arranged by size:
314 212 173 126
PpuMI rG'GwC_Cy
Cuts at: 0 414 825
Size: 414 411
PspOMI G'GGCC_C
Cuts at: 0 187 825
Size: 187 638
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 263 295 825
Size: 263 32 530
Fragments arranged by size:
530 263 32
PstI C_TGCA'G
Cuts at: 0 320 825
Size: 320 505
PvuII CAG'CTG
Cuts at: 0 519 825
Size: 519 306
ScaI AGT'ACT
Cuts at: 0 123 825
Size: 123 702
SfcI C'TryA_G
Cuts at: 0 316 825
Size: 316 509
SmaI CCC'GGG
Cuts at: 0 193 825
Size: 193 632
SpeI A'CTAG_T
Cuts at: 0 66 825
Size: 66 759
StyI C'CwwG_G
Cuts at: 0 445 825
Size: 445 380
TatI w'GTAC_w
Cuts at: 0 121 825
Size: 121 704
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 425 457 599 631 825
Size: 425 32 142 32 194
Fragments arranged by size:
425 194 142 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 181 443 825
Size: 181 262 382
Fragments arranged by size:
382 262 181
XmaI C'CCGG_G
Cuts at: 0 191 825
Size: 191 634
XmnI GAAnn'nnTTC
Cuts at: 0 143 825
Size: 143 682
Enzymes that do cut:
AcuI AflIII AlwNI ApaI ApoI AvaI BamHI BanII
BbvCI BglII Bme1580I BplI BpmI Bpu10I BsaWI BsaXI
BseRI BseYI BsmI Bsp1286I BspEI BspMI BstYI Bsu36I
BtgI BtsI Eco57MI EcoNI EcoO109I EcoRV HincII HindIII
MmeI MspA1I NcoI NspI PasI PciI PfoI PpuMI
PspOMI PsrI PstI PvuII ScaI SfcI SmaI SpeI
StyI TatI TstI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI ApaLI AscI AseI AsiSI
AvrII BaeI BanI BbeI BbsI BcgI BciVI BclI
BfrBI BglI BlpI BmgBI BmrI BmtI BpuEI BsaI
BsaAI BsaBI BsaHI BsgI BsiEI BsiHKAI BsiWI BsmBI
BspHI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstZ17I BtgZI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI EcoRI
FalI FseI FspI FspAI HaeII Hin4I HpaI KasI
KpnI MfeI MluI MscI MslI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI PacI PflMI PmeI
PmlI PpiI PshAI PsiI PspXI PvuI RsrII SacI
SacII SalI SanDI SapI SbfI SexAI SfiI SfoI
SgfI SgrAI SmlI SnaBI SphI SrfI SspI StuI
SwaI TaqII Tth111I XbaI XhoI ZraI