(Circular) (Six-base) MAPSORT of: pFN21AE4176.seq Check: 846 from: 1 to: 6619 pFN21AE4176 6619 bp With 182 enzymes: SgfI * March 28, 2012 14:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3403 3403 Size: 6619 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4429 278 Size: 53 83 186 936 2893 2468 Fragments arranged by size: 2893 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3962 1937 Size: 2025 4594 Acc65I G'GTAC_C Cuts at: 3940 3940 Size: 6619 AclI AA'CG_TT Cuts at: 1563 4746 5119 1563 Size: 3183 373 3063 Fragments arranged by size: 3183 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2008 2183 2657 3000 3749 3929 4682 6066 Size: 376 175 474 343 749 180 753 1384 Cuts at: 6066 6334 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 753 749 474 376 343 268 180 175 AfeI AGC'GCT Cuts at: 2576 2576 Size: 6619 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6422 AflIII A'CryG_T Cuts at: 1203 2394 5518 6495 1203 Size: 1191 3124 977 1327 Fragments arranged by size: 3124 1327 1191 977 AgeI A'CCGG_T Cuts at: 5450 5450 Size: 6619 AhdI GACnn_n'nnGTC Cuts at: 5348 5348 Size: 6619 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6619 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3920 1184 Size: 2736 3883 AlwNI CAG_nnn'CTG Cuts at: 2825 2860 3010 3193 3418 4362 5934 2825 Size: 35 150 183 225 944 1572 3510 Fragments arranged by size: 3510 1572 944 225 183 150 35 ApaI G_GGCC'C Cuts at: 2258 2477 3882 2258 Size: 219 1405 4995 Fragments arranged by size: 4995 1405 219 ApaLI G'TGCA_C Cuts at: 4676 5832 4676 Size: 1156 5463 ApoI r'AATT_y Cuts at: 1493 3928 4154 4306 6415 1493 Size: 2435 226 152 2109 1697 Fragments arranged by size: 2435 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5173 160 Size: 872 4141 1606 Fragments arranged by size: 4141 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6619 AvaI C'yCGr_G Cuts at: 1870 1943 2251 2258 2354 2402 2570 3064 3227 Size: 73 308 7 96 48 168 494 163 Cuts at: 3227 3944 1870 Size: 717 4545 Fragments arranged by size: 4545 717 494 308 168 163 96 73 48 7 BamHI G'GATC_C Cuts at: 3949 4316 3949 Size: 367 6252 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2305 2515 2647 3428 3443 Size: 325 217 627 518 210 132 781 15 Cuts at: 3443 3940 5395 618 Size: 497 1455 1842 Fragments arranged by size: 1842 1455 781 627 518 497 325 217 210 132 15 BanII G_rGCy'C Cuts at: 1391 2184 2252 2258 2301 2477 2956 3075 3219 Size: 793 68 6 43 176 479 119 144 Cuts at: 3219 3525 3816 3882 3938 1391 Size: 306 291 66 56 4072 Fragments arranged by size: 4072 793 479 306 291 176 144 119 68 66 56 43 6 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3510 928 Size: 971 1611 4037 Fragments arranged by size: 4037 1611 971 BbvCI CC'TCA_GC Cuts at: 3155 3155 Size: 6619 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2714 2748 4810 4844 1903 Size: 34 777 34 2062 34 3678 Fragments arranged by size: 3678 2062 777 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4511 5721 4511 Size: 1210 5409 BclI T'GATC_A Cuts at: 1548 1548 Size: 6619 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2406 3543 3753 Size: 107 122 71 631 605 734 1137 210 Cuts at: 3753 5230 136 Size: 1477 1525 Fragments arranged by size: 1525 1477 1137 734 631 605 210 122 107 71 BglII A'GATC_T Cuts at: 6614 6614 Size: 6619 BlpI GC'TnA_GC Cuts at: 2682 3074 3213 4036 2682 Size: 392 139 823 5265 Fragments arranged by size: 5265 823 392 139 Bme1580I G_kGCm'C Cuts at: 1790 2258 2402 2477 2552 3433 3882 4680 5836 Size: 468 144 75 75 881 449 798 1156 Cuts at: 5836 1790 Size: 2573 Fragments arranged by size: 2573 1156 881 798 468 449 144 75 75 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6619 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2316 3463 4350 Size: 416 53 83 399 24 899 1147 887 Cuts at: 4350 5308 442 Size: 958 1753 Fragments arranged by size: 1753 1147 958 899 887 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6619 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3273 3273 Size: 6619 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2675 3053 3632 3869 5279 1239 Size: 1436 378 579 237 1410 2579 Fragments arranged by size: 2579 1436 1410 579 378 237 Bpu10I CC'TnA_GC Cuts at: 2699 2945 3155 2699 Size: 246 210 6163 Fragments arranged by size: 6163 246 210 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3260 3815 3869 4744 5609 5907 3260 Size: 555 54 875 865 298 3972 Fragments arranged by size: 3972 875 865 555 298 54 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5282 882 Size: 617 3783 2219 Fragments arranged by size: 3783 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4410 493 Size: 711 3206 2702 Fragments arranged by size: 3206 2702 711 BsaBI GATnn'nnATC Cuts at: 6613 6613 Size: 6619 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3861 4426 4808 5443 Size: 53 83 186 936 2328 565 382 635 Cuts at: 5443 275 Size: 1451 Fragments arranged by size: 2328 1451 936 635 565 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5052 5450 5724 5871 6282 5052 Size: 398 274 147 411 5389 Fragments arranged by size: 5389 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2531 2561 2582 2612 3026 Size: 30 165 30 1128 30 21 30 414 Cuts at: 3026 3056 3542 3572 1178 Size: 30 486 30 4225 Fragments arranged by size: 4225 1128 486 414 165 30 30 30 30 30 30 21 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2552 2888 3928 1186 Size: 1366 336 1040 3877 Fragments arranged by size: 3877 1366 1040 336 BseYI C'CCAG_C Cuts at: 2309 2728 2860 3418 3477 3541 5822 6318 2309 Size: 419 132 558 59 64 2281 496 2610 Fragments arranged by size: 2610 2281 558 496 419 132 64 59 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2799 3126 1163 Size: 573 1063 327 4656 Fragments arranged by size: 4656 1063 573 327 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2832 3532 3538 4069 4830 5858 Size: 275 186 831 700 6 531 761 1028 Cuts at: 5858 1540 Size: 2301 Fragments arranged by size: 2301 1028 831 761 700 531 275 186 6 BsiHKAI G_wGCw'C Cuts at: 2631 3075 4680 4765 5836 2631 Size: 444 1605 85 1071 3414 Fragments arranged by size: 3414 1605 1071 444 85 BsmI GAATG_Cn' Cuts at: 4130 4223 4130 Size: 93 6526 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2184 2252 2258 2301 2402 2477 2552 Size: 399 394 68 6 43 101 75 75 Cuts at: 2552 2631 2956 3075 3219 3433 3525 3816 3882 Size: 79 325 119 144 214 92 291 66 Cuts at: 3882 3938 4680 4765 5836 1391 Size: 56 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 394 325 291 214 144 119 101 92 85 79 75 75 68 66 56 43 6 BspEI T'CCGG_A Cuts at: 6282 6282 Size: 6619 BspHI T'CATG_A Cuts at: 4508 6238 4508 Size: 1730 4889 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2922 3403 3974 844 Size: 2078 481 571 3489 Fragments arranged by size: 3489 2078 571 481 BsrBI CCG'CTC Cuts at: 1394 1823 2569 2794 2901 4506 1394 Size: 429 746 225 107 1605 3507 Fragments arranged by size: 3507 1605 746 429 225 107 BsrDI GCAATG_nn' Cuts at: 65 1248 2739 5114 5288 65 Size: 1183 1491 2375 174 1396 Fragments arranged by size: 2375 1491 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2059 2138 2529 2808 3537 5263 5450 Size: 110 249 79 391 279 729 1726 187 Cuts at: 5450 1700 Size: 2869 Fragments arranged by size: 2869 1726 729 391 279 249 187 110 79 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6619 BssHII G'CGCG_C Cuts at: 1925 2106 2313 2665 2667 1925 Size: 181 207 352 2 5877 Fragments arranged by size: 5877 352 207 181 2 BssSI C'ACGA_G Cuts at: 2480 2639 4679 5691 2480 Size: 159 2040 1012 3408 Fragments arranged by size: 3408 2040 1012 159 BstAPI GCAn_nnn'nTGC Cuts at: 794 2825 3010 794 Size: 2031 185 4403 Fragments arranged by size: 4403 2031 185 BstBI TT'CG_AA Cuts at: 5423 5423 Size: 6619 BstEII G'GTnAC_C Cuts at: 1184 2909 1184 Size: 1725 4894 BstYI r'GATC_y Cuts at: 1972 3949 4316 4701 4718 6170 6256 6268 6614 Size: 1977 367 385 17 1452 86 12 346 Cuts at: 6614 1972 Size: 1977 Fragments arranged by size: 1977 1977 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2360 2367 2500 2632 2706 3126 Size: 552 939 356 7 133 132 74 420 Cuts at: 3126 3532 3642 513 Size: 406 110 3490 Fragments arranged by size: 3490 939 552 420 406 356 133 132 110 74 7 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5880 Fragments arranged by size: 5880 683 56 BtsI GCAGTG_nn' Cuts at: 2020 3114 3578 4135 4929 4949 2020 Size: 1094 464 557 794 20 3690 Fragments arranged by size: 3690 1094 794 557 464 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6619 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6584 DraI TTT'AAA Cuts at: 3923 3923 Size: 6619 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5626 809 Size: 507 4310 1802 Fragments arranged by size: 4310 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2077 2114 2564 Size: 54 1425 50 275 18 247 37 450 Cuts at: 2564 2635 2829 3123 3498 3535 4066 4955 6584 Size: 71 194 294 375 37 531 889 1629 Cuts at: 6584 8 Size: 43 Fragments arranged by size: 1629 1425 889 531 450 375 294 275 247 194 71 54 50 43 37 37 18 EagI C'GGCC_G Cuts at: 1537 1812 2829 3535 4066 1537 Size: 275 1017 706 531 4090 Fragments arranged by size: 4090 1017 706 531 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2370 3821 3905 4549 1353 Size: 1017 1451 84 644 3423 Fragments arranged by size: 3423 1451 1017 644 84 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2157 2231 5206 5580 5726 387 Size: 1064 138 568 74 2975 374 146 1280 Fragments arranged by size: 2975 1280 1064 568 374 146 138 74 EcoICRI GAG'CTC Cuts at: 3073 3073 Size: 6619 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2008 2183 2657 2675 3000 3053 3632 Size: 393 376 175 474 18 325 53 579 Cuts at: 3632 3749 3869 3929 4682 5279 6066 6334 1239 Size: 117 120 60 753 597 787 268 1524 Fragments arranged by size: 1524 787 753 597 579 474 393 376 325 268 175 120 117 60 53 18 EcoO109I rG'GnC_Cy Cuts at: 2255 2974 3879 2255 Size: 719 905 4995 Fragments arranged by size: 4995 905 719 EcoRI G'AATT_C Cuts at: 3928 4306 3928 Size: 378 6241 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3856 3856 Size: 6619 FspI TGC'GCA Cuts at: 5125 5125 Size: 6619 HaeII r_GCGC'y Cuts at: 1678 2578 2706 5766 1678 Size: 900 128 3060 2531 Fragments arranged by size: 3060 2531 900 128 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 5260 5292 5334 5366 1373 Size: 32 135 32 3688 32 42 32 2626 Fragments arranged by size: 3688 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3139 3963 4209 669 Size: 988 281 1201 824 246 3079 Fragments arranged by size: 3079 1201 988 824 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6619 HpaI GTT'AAC Cuts at: 4209 4209 Size: 6619 KpnI G_GTAC'C Cuts at: 3944 3944 Size: 6619 MfeI C'AATT_G Cuts at: 4218 4218 Size: 6619 MluI A'CGCG_T Cuts at: 2394 6495 2394 Size: 4101 2518 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5733 5917 1305 Size: 4428 184 2007 Fragments arranged by size: 4428 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4797 Fragments arranged by size: 4797 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3902 4577 4936 5095 518 Size: 648 2736 675 359 159 2042 Fragments arranged by size: 2736 2042 675 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2028 2163 2170 2369 2502 2524 2634 2653 Size: 422 135 7 199 133 22 110 19 Cuts at: 2653 3154 3331 3534 4035 4712 5860 6105 1606 Size: 501 177 203 501 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 501 501 422 245 203 199 177 135 133 110 22 19 7 NaeI GCC'GGC Cuts at: 2140 2531 2810 3539 2140 Size: 391 279 729 5220 Fragments arranged by size: 5220 729 391 279 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5128 Fragments arranged by size: 5128 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6619 NgoMIV G'CCGG_C Cuts at: 2138 2529 2808 3537 2138 Size: 391 279 729 5220 Fragments arranged by size: 5220 729 391 279 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6619 NotI GC'GGCC_GC Cuts at: 2829 4066 2829 Size: 1237 5382 NspI r_CATG'y Cuts at: 1132 1229 3121 3977 5522 1132 Size: 97 1892 856 1545 2229 Fragments arranged by size: 2229 1892 1545 856 97 PasI CC'CwG_GG Cuts at: 1348 2905 3050 3261 1348 Size: 1557 145 211 4706 Fragments arranged by size: 4706 1557 211 145 PciI A'CATG_T Cuts at: 5518 5518 Size: 6619 PflMI CCAn_nnn'nTGG Cuts at: 1108 2644 2860 1108 Size: 1536 216 4867 Fragments arranged by size: 4867 1536 216 PfoI T'CCnGG_A Cuts at: 2052 2328 3846 2052 Size: 276 1518 4825 Fragments arranged by size: 4825 1518 276 PmeI GTTT'AAAC Cuts at: 3923 3923 Size: 6619 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4688 4720 6201 6233 4688 Size: 32 1481 32 5074 Fragments arranged by size: 5074 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2974 2974 Size: 6619 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6619 PsiI TTA'TAA Cuts at: 4189 4189 Size: 6619 PspOMI G'GGCC_C Cuts at: 2254 2473 3878 2254 Size: 219 1405 4995 Fragments arranged by size: 4995 1405 219 PspXI vC'TCGA_Gb Cuts at: 2570 2570 Size: 6619 PstI C_TGCA'G Cuts at: 830 2238 2841 3006 3330 3573 3971 830 Size: 1408 603 165 324 243 398 3478 Fragments arranged by size: 3478 1408 603 398 324 243 165 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6619 PvuII CAG'CTG Cuts at: 2028 3154 3331 2028 Size: 1126 177 5316 Fragments arranged by size: 5316 1126 177 SacI G_AGCT'C Cuts at: 3075 3075 Size: 6619 SacII CC_GC'GG Cuts at: 2370 2503 2635 3535 2370 Size: 133 132 900 5454 Fragments arranged by size: 5454 900 133 132 SalI G'TCGA_C Cuts at: 1936 3961 1936 Size: 2025 4594 SapI GCTCTTCn'nnn_ Cuts at: 2370 3821 2370 Size: 1451 5168 SbfI CC_TGCA'GG Cuts at: 2841 3006 3971 2841 Size: 165 965 5489 Fragments arranged by size: 5489 965 165 ScaI AGT'ACT Cuts at: 4867 6579 4867 Size: 1712 4907 SfcI C'TryA_G Cuts at: 826 1045 2234 2837 3002 3326 3569 3967 5102 Size: 219 1189 603 165 324 243 398 1135 Cuts at: 5102 5783 5974 826 Size: 681 191 1471 Fragments arranged by size: 1471 1189 1135 681 603 398 324 243 219 191 165 SfiI GGCCn_nnn'nGGCC Cuts at: 3543 3543 Size: 6619 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6619 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6619 SmaI CCC'GGG Cuts at: 1872 2260 3946 1872 Size: 388 1686 4545 Fragments arranged by size: 4545 1686 388 SmlI C'TyrA_G Cuts at: 820 1017 1943 2570 3227 3275 3830 3884 4723 Size: 197 926 627 657 48 555 54 839 Cuts at: 4723 5624 5886 820 Size: 901 262 1553 Fragments arranged by size: 1553 926 901 839 657 627 555 262 197 54 48 SnaBI TAC'GTA Cuts at: 493 4410 493 Size: 3917 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6619 SphI G_CATG'C Cuts at: 1132 3977 1132 Size: 2845 3774 SspI AAT'ATT Cuts at: 5 52 4543 5 Size: 47 4491 2081 Fragments arranged by size: 4491 2081 47 StuI AGG'CCT Cuts at: 1517 2612 3734 1517 Size: 1095 1122 4402 Fragments arranged by size: 4402 1122 1095 StyI C'CwwG_G Cuts at: 513 1065 2004 3678 4058 513 Size: 552 939 1674 380 3074 Fragments arranged by size: 3074 1674 939 552 380 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2323 2720 3030 3416 3453 3836 4646 Size: 1255 743 397 310 386 37 383 810 Cuts at: 4646 4663 4816 5001 5446 325 Size: 17 153 185 445 1498 Fragments arranged by size: 1498 1255 810 743 445 397 386 383 310 185 153 37 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4865 6577 96 Size: 274 80 33 51 1443 2888 1712 138 Fragments arranged by size: 2888 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2207 2239 2531 2563 2582 2614 3026 3058 6579 Size: 32 292 32 19 32 412 32 3521 Cuts at: 6579 6611 2207 Size: 32 2215 Fragments arranged by size: 3521 2215 412 292 32 32 32 32 32 19 Tth111I GACn'n_nGTC Cuts at: 1316 2990 1316 Size: 1674 4945 XbaI T'CTAG_A Cuts at: 3955 3955 Size: 6619 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2758 2758 Size: 6619 XhoI C'TCGA_G Cuts at: 1943 2570 3227 1943 Size: 627 657 5335 Fragments arranged by size: 5335 657 627 XmaI C'CCGG_G Cuts at: 1870 2258 3944 1870 Size: 388 1686 4545 Fragments arranged by size: 4545 1686 388 XmnI GAAnn'nnTTC Cuts at: 4748 4748 Size: 6619 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4427 276 Size: 53 83 186 936 2893 2468 Fragments arranged by size: 2893 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PmlI PsrI RsrII SanDI SexAI SfoI SrfI SwaI