(Linear) (Six-base) MAPSORT of: KIEE4176.seq Check: 1692 from: 1 to: 1914 KIEE4176.orf With 182 enzymes: SgfI * March 22, 2012 17:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1398 1914 Size: 1398 516 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 3 178 652 995 1744 1914 Size: 3 175 474 343 749 170 Fragments arranged by size: 749 474 343 175 170 3 AfeI AGC'GCT Cuts at: 0 571 1914 Size: 571 1343 AflIII A'CryG_T Cuts at: 0 389 1914 Size: 389 1525 AlwNI CAG_nnn'CTG Cuts at: 0 820 855 1005 1188 1413 1914 Size: 820 35 150 183 225 501 Fragments arranged by size: 820 501 225 183 150 35 ApaI G_GGCC'C Cuts at: 0 253 472 1877 1914 Size: 253 219 1405 37 Fragments arranged by size: 1405 253 219 37 AvaI C'yCGr_G Cuts at: 0 246 253 349 397 565 1059 1222 1914 Size: 246 7 96 48 168 494 163 692 Fragments arranged by size: 692 494 246 168 163 96 48 7 BanI G'GyrC_C Cuts at: 0 300 510 642 1423 1438 1914 Size: 300 210 132 781 15 476 Fragments arranged by size: 781 476 300 210 132 15 BanII G_rGCy'C Cuts at: 0 179 247 253 296 472 951 1070 1214 Size: 179 68 6 43 176 479 119 144 Cuts at: 1214 1520 1811 1877 1914 Size: 306 291 66 37 Fragments arranged by size: 479 306 291 179 176 144 119 68 66 43 37 6 BbsI GAAGACnn'nnnn_ Cuts at: 0 1505 1914 Size: 1505 409 BbvCI CC'TCA_GC Cuts at: 0 1150 1914 Size: 1150 764 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 709 743 1914 Size: 709 34 1171 Fragments arranged by size: 1171 709 34 BglI GCCn_nnn'nGGC Cuts at: 0 401 1538 1748 1914 Size: 401 1137 210 166 Fragments arranged by size: 1137 401 210 166 BlpI GC'TnA_GC Cuts at: 0 677 1069 1208 1914 Size: 677 392 139 706 Fragments arranged by size: 706 677 392 139 Bme1580I G_kGCm'C Cuts at: 0 253 397 472 547 1428 1877 1914 Size: 253 144 75 75 881 449 37 Fragments arranged by size: 881 449 253 144 75 75 37 BmrI ACTGGGnnnn_n' Cuts at: 0 311 1458 1914 Size: 311 1147 456 Fragments arranged by size: 1147 456 311 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1268 1914 Size: 1268 646 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 670 1048 1627 1864 1914 Size: 670 378 579 237 50 Fragments arranged by size: 670 579 378 237 50 Bpu10I CC'TnA_GC Cuts at: 0 694 940 1150 1914 Size: 694 246 210 764 Fragments arranged by size: 764 694 246 210 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1255 1810 1864 1914 Size: 1255 555 54 50 Fragments arranged by size: 1255 555 54 50 BsaHI Gr'CG_yC Cuts at: 0 1856 1914 Size: 1856 58 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 526 556 577 607 1021 1051 1537 1567 Size: 526 30 21 30 414 30 486 30 Cuts at: 1567 1914 Size: 347 Fragments arranged by size: 526 486 414 347 30 30 30 30 21 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 547 883 1914 Size: 547 336 1031 Fragments arranged by size: 1031 547 336 BseYI C'CCAG_C Cuts at: 0 304 723 855 1413 1472 1536 1914 Size: 304 419 132 558 59 64 378 Fragments arranged by size: 558 419 378 304 132 64 59 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 794 1121 1914 Size: 794 327 793 Fragments arranged by size: 794 793 327 BsiEI CG_ry'CG Cuts at: 0 827 1527 1533 1914 Size: 827 700 6 381 Fragments arranged by size: 827 700 381 6 BsiHKAI G_wGCw'C Cuts at: 0 626 1070 1914 Size: 626 444 844 Fragments arranged by size: 844 626 444 Bsp1286I G_dGCh'C Cuts at: 0 179 247 253 296 397 472 547 626 Size: 179 68 6 43 101 75 75 79 Cuts at: 626 951 1070 1214 1428 1520 1811 1877 1914 Size: 325 119 144 214 92 291 66 37 Fragments arranged by size: 325 291 214 179 144 119 101 92 79 75 75 68 66 43 37 6 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 917 1398 1914 Size: 917 481 516 Fragments arranged by size: 917 516 481 BsrBI CCG'CTC Cuts at: 0 564 789 896 1914 Size: 564 225 107 1018 Fragments arranged by size: 1018 564 225 107 BsrDI GCAATG_nn' Cuts at: 0 734 1914 Size: 734 1180 BsrFI r'CCGG_y Cuts at: 0 54 133 524 803 1532 1914 Size: 54 79 391 279 729 382 Fragments arranged by size: 729 391 382 279 79 54 BssHII G'CGCG_C Cuts at: 0 101 308 660 662 1914 Size: 101 207 352 2 1252 Fragments arranged by size: 1252 352 207 101 2 BssSI C'ACGA_G Cuts at: 0 475 634 1914 Size: 475 159 1280 Fragments arranged by size: 1280 475 159 BstAPI GCAn_nnn'nTGC Cuts at: 0 820 1005 1914 Size: 820 185 909 Fragments arranged by size: 909 820 185 BstEII G'GTnAC_C Cuts at: 0 904 1914 Size: 904 1010 BtgI C'CryG_G Cuts at: 0 355 362 495 627 701 1121 1527 1637 Size: 355 7 133 132 74 420 406 110 Cuts at: 1637 1914 Size: 277 Fragments arranged by size: 420 406 355 277 133 132 110 74 7 BtsI GCAGTG_nn' Cuts at: 0 15 1109 1573 1914 Size: 15 1094 464 341 Fragments arranged by size: 1094 464 341 15 EaeI y'GGCC_r Cuts at: 0 72 109 559 630 824 1118 1493 1530 Size: 72 37 450 71 194 294 375 37 Cuts at: 1530 1914 Size: 384 Fragments arranged by size: 450 384 375 294 194 72 71 37 37 EagI C'GGCC_G Cuts at: 0 824 1530 1914 Size: 824 706 384 Fragments arranged by size: 824 706 384 EarI CTCTTCn'nnn_ Cuts at: 0 365 1816 1900 1914 Size: 365 1451 84 14 Fragments arranged by size: 1451 365 84 14 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 152 226 1914 Size: 152 74 1688 Fragments arranged by size: 1688 152 74 EcoICRI GAG'CTC Cuts at: 0 1068 1914 Size: 1068 846 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 3 178 652 670 995 1048 1627 1744 Size: 3 175 474 18 325 53 579 117 Cuts at: 1744 1864 1914 Size: 120 50 Fragments arranged by size: 579 474 325 175 120 117 53 50 18 3 EcoO109I rG'GnC_Cy Cuts at: 0 250 969 1874 1914 Size: 250 719 905 40 Fragments arranged by size: 905 719 250 40 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1851 1914 Size: 1851 63 HaeII r_GCGC'y Cuts at: 0 573 701 1914 Size: 573 128 1213 Fragments arranged by size: 1213 573 128 HincII GTy'rAC Cuts at: 0 1134 1914 Size: 1134 780 MluI A'CGCG_T Cuts at: 0 389 1914 Size: 389 1525 MslI CAynn'nnrTG Cuts at: 0 1897 1914 Size: 1897 17 MspA1I CmG'CkG Cuts at: 0 23 158 165 364 497 519 629 648 Size: 23 135 7 199 133 22 110 19 Cuts at: 648 1149 1326 1529 1914 Size: 501 177 203 385 Fragments arranged by size: 501 385 203 199 177 135 133 110 23 22 19 7 NaeI GCC'GGC Cuts at: 0 135 526 805 1534 1914 Size: 135 391 279 729 380 Fragments arranged by size: 729 391 380 279 135 NgoMIV G'CCGG_C Cuts at: 0 133 524 803 1532 1914 Size: 133 391 279 729 382 Fragments arranged by size: 729 391 382 279 133 NotI GC'GGCC_GC Cuts at: 0 824 1914 Size: 824 1090 NspI r_CATG'y Cuts at: 0 1116 1914 Size: 1116 798 PasI CC'CwG_GG Cuts at: 0 900 1045 1256 1914 Size: 900 145 211 658 Fragments arranged by size: 900 658 211 145 PflMI CCAn_nnn'nTGG Cuts at: 0 639 855 1914 Size: 639 216 1059 Fragments arranged by size: 1059 639 216 PfoI T'CCnGG_A Cuts at: 0 47 323 1841 1914 Size: 47 276 1518 73 Fragments arranged by size: 1518 276 73 47 PpuMI rG'GwC_Cy Cuts at: 0 969 1914 Size: 969 945 PspOMI G'GGCC_C Cuts at: 0 249 468 1873 1914 Size: 249 219 1405 41 Fragments arranged by size: 1405 249 219 41 PspXI vC'TCGA_Gb Cuts at: 0 565 1914 Size: 565 1349 PstI C_TGCA'G Cuts at: 0 233 836 1001 1325 1568 1914 Size: 233 603 165 324 243 346 Fragments arranged by size: 603 346 324 243 233 165 PvuII CAG'CTG Cuts at: 0 23 1149 1326 1914 Size: 23 1126 177 588 Fragments arranged by size: 1126 588 177 23 SacI G_AGCT'C Cuts at: 0 1070 1914 Size: 1070 844 SacII CC_GC'GG Cuts at: 0 365 498 630 1530 1914 Size: 365 133 132 900 384 Fragments arranged by size: 900 384 365 133 132 SapI GCTCTTCn'nnn_ Cuts at: 0 365 1816 1914 Size: 365 1451 98 Fragments arranged by size: 1451 365 98 SbfI CC_TGCA'GG Cuts at: 0 836 1001 1914 Size: 836 165 913 Fragments arranged by size: 913 836 165 SfcI C'TryA_G Cuts at: 0 229 832 997 1321 1564 1914 Size: 229 603 165 324 243 350 Fragments arranged by size: 603 350 324 243 229 165 SfiI GGCCn_nnn'nGGCC Cuts at: 0 1538 1914 Size: 1538 376 SmaI CCC'GGG Cuts at: 0 255 1914 Size: 255 1659 SmlI C'TyrA_G Cuts at: 0 565 1222 1270 1825 1879 1914 Size: 565 657 48 555 54 35 Fragments arranged by size: 657 565 555 54 48 35 StuI AGG'CCT Cuts at: 0 607 1729 1914 Size: 607 1122 185 Fragments arranged by size: 1122 607 185 StyI C'CwwG_G Cuts at: 0 1673 1914 Size: 1673 241 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 318 715 1025 1411 1448 1831 1914 Size: 318 397 310 386 37 383 83 Fragments arranged by size: 397 386 383 318 310 83 37 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 202 234 526 558 577 609 1021 1053 Size: 202 32 292 32 19 32 412 32 Cuts at: 1053 1914 Size: 861 Fragments arranged by size: 861 412 292 202 32 32 32 32 19 Tth111I GACn'n_nGTC Cuts at: 0 985 1914 Size: 985 929 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 753 1914 Size: 753 1161 XhoI C'TCGA_G Cuts at: 0 565 1222 1914 Size: 565 657 692 Fragments arranged by size: 692 657 565 XmaI C'CCGG_G Cuts at: 0 253 1914 Size: 253 1661 Enzymes that do cut: AarI AcuI AfeI AflIII AlwNI ApaI AvaI BanI BanII BbsI BbvCI BcgI BglI BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstEII BtgI BtsI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I FalI HaeII HincII MluI MslI MspA1I NaeI NgoMIV NotI NspI PasI PflMI PfoI PpuMI PspOMI PspXI PstI PvuII SacI SacII SapI SbfI SfcI SfiI SmaI SmlI StuI StyI TaqII TstI Tth111I XcmI XhoI XmaI Enzymes that do not cut: AatII AccI Acc65I AclI AflII AgeI AhdI AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI BciVI BclI BfrBI BglII BmgBI BmtI BsaI BsaAI BsaBI BsaWI BsiWI BsmI BsmBI BspEI BspHI BsrGI BstBI BstXI BstYI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EcoNI EcoRI EcoRV FseI FspI FspAI Hin4I HindIII HpaI KasI KpnI MfeI MmeI MscI NarI NcoI NdeI NheI NruI NsiI PacI PciI PmeI PmlI PpiI PshAI PsiI PsrI PvuI RsrII SalI SanDI ScaI SexAI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI TatI XbaI XmnI ZraI