################################## # NO CUT ENZYMES ( 159 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspHI AspI AviII AvrII BalI BbeI BbrPI BcgI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BsiHKAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraIII Eam1105I EarI Eco47III Eco81I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MamI MfeI MluI MluNI MroI MscI MseI MslI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tru9I Tsp509I Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### ApaI 75 GGGCCC GGGCC^C Asp700I 787 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaI 851 CTCGGG C^[CT]CG[GA]G BamHI 88 GGATCC G^GATCC BbsI 891 GAAGAC GAAGAC(2/6) BbvCI 249 CCTCAGC CCTCAGC(-5/-2) BciVI 315 GGATAC GTATCC(6/5) BcnI 375 CCCGG CC^[GC]GG BfaI 595 CTAG C^TAG BpuAI 891 GAAGAC GAAGAC(2/6) BsaI 187 GAGACC GGTCTC(1/5) BsiEI 216 CGGCCG CG[GA][CT]^CG BsmAI 187 GAGAC GTCTC(1/5) BsoBI 851 CTCGGG C^[CT]CG[GA]G BspHI 339 TCATGA T^CATGA BspMI 396 ACCTGC ACCTGC(4/8) BstAPI 472 GCAGTGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 631 GGTGACC G^GT[ACGT]ACC BstPI 631 GGTGACC G^GT[ACGT]ACC BstYI 88 GGATCC [GA]^GATC[CT] DrdI 189 GACCAAAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 216 CGGCCG C^GGCCG EclXI 216 CGGCCG C^GGCCG Eco52I 216 CGGCCG C^GGCCG EcoNI 776 CCTCCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 631 GGTGACC G^GT[ACGT]ACC HapII 376 CCGG C^CGG HpaII 376 CCGG C^CGG MaeI 595 CTAG C^TAG MaeII 385 ACGT A^CGT MflI 88 GGATCC [GA]^GATC[CT] MspA1I 538 CCGCTG C[AC]G^C[GT]G MspI 376 CCGG C^CGG NciI 375 CCCGG CC^[GC]GG NheI 594 GCTAGC G^CTAGC NlaIII 340 CATG CATG^ PstI 721 CTGCAG CTGCA^G RcaI 339 TCATGA T^CATGA SfcI 721 CTGCAG C^T[GA][CT]AG StuI 847 AGGCCT AGG^CCT TaqI 353 TCGA T^CGA TthHB8I 353 TCGA T^CGA XhoII 88 GGATCC [GA]^GATC[CT] XmnI 787 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 12 enzymes) # ################################### BanI 295 GGCACC G^G[CT][GA]CC 478 GGTGCC BanII 14 GGGCTC G[GA]GC[CT]^C 75 GGGCCC BmyI 14 GGGCTC G[AGT]GC[ACT]^C 75 GGGCCC Bsp1286I 14 GGGCTC G[AGT]GC[ACT]^C 75 GGGCCC BsrI 143 ACTGG ACTGG(1/-1) 302 ACTGG EaeI 216 CGGCCG [CT]^GGCC[GA] 413 CGGCCA EcoT14I 10 CCTTGG C^C[AT][AT]GG 742 CCAAGG FauI 537 CCCGC CCCGC(4/6) 73 GCGGG HphI 522 GGTGA GGTGA(8/7) 631 GGTGA MboII 787 GAAGA GAAGA(8/7) 891 GAAGA PpuMI 32 GGGTCCC [GA]G^G[AT]CC[CT] 127 AGGACCC StyI 10 CCTTGG C^C[AT][AT]GG 742 CCAAGG ######################################### # 3 OR MORE CUT ENZYMES ( 43 enzymes) # ######################################### AciI AluI AlwI AvaII BbvI BpmI BsaJI BseRI BsgI BsiYI BslI BsmFI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII DraII DsaI EcoO109I EcoRII Fnu4HI FokI HaeIII HinfI ItaI MaeIII MboI MnlI MvaI MwoI NdeII NlaIV Sau3AI Sau96I ScrFI SfaNI TfiI TseI Tsp45I TspRI