(Circular) (Six-base) MAPSORT of: pFN21AE4107.seq Check: 9280 from: 1 to: 6139 pFN21AE4107 6139 bp With 182 enzymes: SgfI * March 28, 2012 13:39 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3396 3396 Size: 6139 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2922 3949 278 Size: 53 83 186 936 1386 1027 2468 Fragments arranged by size: 2468 1386 1027 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3411 3482 1937 Size: 1474 71 4594 Fragments arranged by size: 4594 1474 71 Acc65I G'GTAC_C Cuts at: 2367 2520 3460 2367 Size: 153 940 5046 Fragments arranged by size: 5046 940 153 AclI AA'CG_TT Cuts at: 1563 4266 4639 1563 Size: 2703 373 3063 Fragments arranged by size: 3063 2703 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4202 5586 5854 1632 Size: 2570 1384 268 1917 Fragments arranged by size: 2570 1917 1384 268 AfeI AGC'GCT Cuts at: 2159 2159 Size: 6139 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5942 AflIII A'CryG_T Cuts at: 1203 5038 6015 1203 Size: 3835 977 1327 Fragments arranged by size: 3835 1327 977 AgeI A'CCGG_T Cuts at: 4970 4970 Size: 6139 AhdI GACnn_n'nnGTC Cuts at: 4868 4868 Size: 6139 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6139 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3161 1184 Size: 1977 4162 AlwNI CAG_nnn'CTG Cuts at: 2049 2170 2578 2653 3882 5454 2049 Size: 121 408 75 1229 1572 2734 Fragments arranged by size: 2734 1572 1229 408 121 75 ApaI G_GGCC'C Cuts at: 2507 3038 2507 Size: 531 5608 ApaLI G'TGCA_C Cuts at: 4196 5352 4196 Size: 1156 4983 ApoI r'AATT_y Cuts at: 1493 3448 3674 3826 5935 1493 Size: 1955 226 152 2109 1697 Fragments arranged by size: 2109 1955 1697 226 152 AscI GG'CGCG_CC Cuts at: 2831 3254 2831 Size: 423 5716 AseI AT'TA_AT Cuts at: 160 1032 4693 160 Size: 872 3661 1606 Fragments arranged by size: 3661 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6139 AvaI C'yCGr_G Cuts at: 1870 1943 2633 3037 3464 1870 Size: 73 690 404 427 4545 Fragments arranged by size: 4545 690 427 404 73 AvrII C'CTAG_G Cuts at: 2009 2009 Size: 6139 BamHI G'GATC_C Cuts at: 3469 3836 3469 Size: 367 5772 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2123 2222 2367 2520 2537 Size: 325 217 627 336 99 145 153 17 Cuts at: 2537 2621 2969 3460 4915 618 Size: 84 348 491 1455 1842 Fragments arranged by size: 1842 1455 627 491 348 336 325 217 153 145 99 84 17 BanII G_rGCy'C Cuts at: 1391 2507 2633 3038 3458 1391 Size: 1116 126 405 420 4072 Fragments arranged by size: 4072 1116 420 405 126 BbeI G_GCGC'C Cuts at: 2625 2973 2625 Size: 348 5791 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2341 3242 928 Size: 971 442 901 3825 Fragments arranged by size: 3825 971 901 442 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2935 2969 4330 4364 1903 Size: 34 50 34 914 34 1361 34 3678 Fragments arranged by size: 3678 1361 914 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4031 5241 4031 Size: 1210 4929 BclI T'GATC_A Cuts at: 1548 1548 Size: 6139 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2603 2753 3042 Size: 107 122 71 631 605 931 150 289 Cuts at: 3042 3094 3264 4750 136 Size: 52 170 1486 1525 Fragments arranged by size: 1525 1486 931 631 605 289 170 150 122 107 71 52 BglII A'GATC_T Cuts at: 6134 6134 Size: 6139 BlpI GC'TnA_GC Cuts at: 3556 3556 Size: 6139 Bme1580I G_kGCm'C Cuts at: 1790 2225 2507 2540 3038 3140 4200 5356 1790 Size: 435 282 33 498 102 1060 1156 2573 Fragments arranged by size: 2573 1156 1060 498 435 282 102 33 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6139 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3870 4828 442 Size: 416 53 83 399 24 2453 958 1753 Fragments arranged by size: 2453 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6139 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2138 2309 2708 2726 4799 1239 Size: 899 171 399 18 2073 2579 Fragments arranged by size: 2579 2073 899 399 171 18 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4264 5129 5427 4264 Size: 865 298 4976 Fragments arranged by size: 4976 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4802 882 Size: 617 3303 2219 Fragments arranged by size: 3303 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3930 493 Size: 711 2726 2702 Fragments arranged by size: 2726 2702 711 BsaBI GATnn'nnATC Cuts at: 6133 6133 Size: 6139 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2622 2919 2970 3129 Size: 53 83 186 936 1089 297 51 159 Cuts at: 3129 3191 3946 4328 4963 275 Size: 62 755 382 635 1451 Fragments arranged by size: 1451 1089 936 755 635 382 297 186 159 83 62 53 51 BsaWI w'CCGG_w Cuts at: 3426 4572 4970 5244 5391 5802 3426 Size: 1146 398 274 147 411 3763 Fragments arranged by size: 3763 1146 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5914 Fragments arranged by size: 5914 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3326 1186 Size: 2140 3999 BseYI C'CCAG_C Cuts at: 2263 2716 3031 5342 5838 2263 Size: 453 315 2311 496 2564 Fragments arranged by size: 2564 2311 496 453 315 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2231 2255 2402 2882 3368 3374 1163 Size: 573 495 24 147 480 486 6 3928 Fragments arranged by size: 3928 573 495 486 480 147 24 6 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2598 2732 3007 3172 3176 3589 Size: 275 186 597 134 275 165 4 413 Cuts at: 3589 4350 5378 1540 Size: 761 1028 2301 Fragments arranged by size: 2301 1028 761 597 413 275 275 186 165 134 4 BsiHKAI G_wGCw'C Cuts at: 2154 3081 4200 4285 5356 2154 Size: 927 1119 85 1071 2937 Fragments arranged by size: 2937 1119 1071 927 85 BsmI GAATG_Cn' Cuts at: 3650 3743 3650 Size: 93 6046 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2154 2225 2507 2540 2633 3038 3081 Size: 399 364 71 282 33 93 405 43 Cuts at: 3081 3140 3458 4200 4285 5356 1391 Size: 59 318 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 405 399 364 318 282 93 85 71 59 43 33 BspEI T'CCGG_A Cuts at: 3426 5802 3426 Size: 2376 3763 BspHI T'CATG_A Cuts at: 4028 5758 4028 Size: 1730 4409 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3351 3396 3494 844 Size: 2507 45 98 3489 Fragments arranged by size: 3489 2507 98 45 BsrBI CCG'CTC Cuts at: 1394 1823 2752 4026 1394 Size: 429 929 1274 3507 Fragments arranged by size: 3507 1274 929 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4634 4808 65 Size: 1183 3386 174 1396 Fragments arranged by size: 3386 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2788 3193 3317 4783 4970 1700 Size: 110 978 405 124 1466 187 2869 Fragments arranged by size: 2869 1466 978 405 187 124 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6139 BssHII G'CGCG_C Cuts at: 1925 2479 2481 2724 2761 2813 2831 3155 3254 Size: 554 2 243 37 52 18 324 99 Cuts at: 3254 1925 Size: 4810 Fragments arranged by size: 4810 554 324 243 99 52 37 18 2 BssSI C'ACGA_G Cuts at: 4199 5211 4199 Size: 1012 5127 BstAPI GCAn_nnn'nTGC Cuts at: 794 2170 2653 794 Size: 1376 483 4280 Fragments arranged by size: 4280 1376 483 BstBI TT'CG_AA Cuts at: 4943 4943 Size: 6139 BstEII G'GTnAC_C Cuts at: 1184 2077 3064 1184 Size: 893 987 4259 Fragments arranged by size: 4259 987 893 BstXI CCAn_nnnn'nTGG Cuts at: 2197 2197 Size: 6139 BstYI r'GATC_y Cuts at: 1972 3469 3836 4221 4238 5690 5776 5788 6134 Size: 1497 367 385 17 1452 86 12 346 Cuts at: 6134 1972 Size: 1977 Fragments arranged by size: 1977 1497 1452 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 3412 3412 Size: 6139 BtgI C'CryG_G Cuts at: 513 1065 2598 2742 2836 2844 2975 3132 3176 Size: 552 1533 144 94 8 131 157 44 Cuts at: 3176 513 Size: 3476 Fragments arranged by size: 3476 1533 552 157 144 131 94 44 8 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2208 487 Size: 683 56 982 4418 Fragments arranged by size: 4418 982 683 56 BtsI GCAGTG_nn' Cuts at: 3138 3655 4449 4469 3138 Size: 517 794 20 4808 Fragments arranged by size: 4808 794 517 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6139 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6104 DraI TTT'AAA Cuts at: 3443 3443 Size: 6139 DraIII CAC_nnn'GTG Cuts at: 2616 2616 Size: 6139 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5146 809 Size: 507 3830 1802 Fragments arranged by size: 3830 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2427 2466 2595 Size: 54 1425 50 275 18 597 39 129 Cuts at: 2595 2604 2729 2745 2850 3043 3169 3195 3586 Size: 9 125 16 105 193 126 26 391 Cuts at: 3586 4475 6104 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 597 391 275 193 129 126 125 105 54 50 43 39 26 18 16 9 EagI C'GGCC_G Cuts at: 1537 1812 2595 2729 3169 3586 1537 Size: 275 783 134 440 417 4090 Fragments arranged by size: 4090 783 440 417 275 134 EarI CTCTTCn'nnn_ Cuts at: 1353 3086 4069 1353 Size: 1733 983 3423 Fragments arranged by size: 3423 1733 983 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3269 4726 5100 5246 387 Size: 1064 138 1680 1457 374 146 1280 Fragments arranged by size: 1680 1457 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2138 2309 2708 2726 4202 4799 5586 Size: 393 506 171 399 18 1476 597 787 Cuts at: 5586 5854 1239 Size: 268 1524 Fragments arranged by size: 1524 1476 787 597 506 399 393 268 171 18 EcoNI CCTnn'n_nnAGG Cuts at: 2013 2864 2013 Size: 851 5288 EcoO109I rG'GnC_Cy Cuts at: 2034 2551 2034 Size: 517 5622 EcoRI G'AATT_C Cuts at: 3448 3826 3448 Size: 378 5761 FspI TGC'GCA Cuts at: 4645 4645 Size: 6139 HaeII r_GCGC'y Cuts at: 1678 2161 2625 2759 2934 2973 5286 1678 Size: 483 464 134 175 39 2313 2531 Fragments arranged by size: 2531 2313 483 464 175 134 39 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4780 4812 4854 4886 1373 Size: 32 135 32 3208 32 42 32 2626 Fragments arranged by size: 3208 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2923 3483 3729 669 Size: 988 281 985 560 246 3079 Fragments arranged by size: 3079 988 985 560 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6139 HpaI GTT'AAC Cuts at: 3729 3729 Size: 6139 KasI G'GCGC_C Cuts at: 2621 2969 2621 Size: 348 5791 KpnI G_GTAC'C Cuts at: 2371 2524 3464 2371 Size: 153 940 5046 Fragments arranged by size: 5046 940 153 MfeI C'AATT_G Cuts at: 3738 3738 Size: 6139 MluI A'CGCG_T Cuts at: 6015 6015 Size: 6139 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5253 5437 1305 Size: 3948 184 2007 Fragments arranged by size: 3948 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2429 3045 10 Size: 54 1425 343 597 616 3104 Fragments arranged by size: 3104 1425 616 597 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4097 4456 4615 518 Size: 648 2931 359 159 2042 Fragments arranged by size: 2931 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2120 2345 2797 2806 2846 2896 3031 3134 Size: 514 225 452 9 40 50 135 103 Cuts at: 3134 3178 3204 3349 3555 4232 5380 5625 1606 Size: 44 26 145 206 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 514 452 245 225 206 145 135 103 50 44 40 26 9 NaeI GCC'GGC Cuts at: 3195 3319 3195 Size: 124 6015 NarI GG'CG_CC Cuts at: 2622 2970 2622 Size: 348 5791 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5587 NdeI CA'TA_TG Cuts at: 387 387 Size: 6139 NgoMIV G'CCGG_C Cuts at: 3193 3317 3193 Size: 124 6015 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6139 NotI GC'GGCC_GC Cuts at: 2729 3586 2729 Size: 857 5282 NspI r_CATG'y Cuts at: 1132 1229 2479 3497 5042 1132 Size: 97 1250 1018 1545 2229 Fragments arranged by size: 2229 1545 1250 1018 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6139 PciI A'CATG_T Cuts at: 5038 5038 Size: 6139 PflMI CCAn_nnn'nTGG Cuts at: 1108 2704 1108 Size: 1596 4543 PfoI T'CCnGG_A Cuts at: 2286 2286 Size: 6139 PmeI GTTT'AAAC Cuts at: 3443 3443 Size: 6139 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4208 4240 5721 5753 4208 Size: 32 1481 32 4594 Fragments arranged by size: 4594 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2034 2034 Size: 6139 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6139 PsiI TTA'TAA Cuts at: 3709 3709 Size: 6139 PspOMI G'GGCC_C Cuts at: 2503 3034 2503 Size: 531 5608 PstI C_TGCA'G Cuts at: 830 2325 2457 2574 2679 2811 2868 3348 3491 Size: 1495 132 117 105 132 57 480 143 Cuts at: 3491 830 Size: 3478 Fragments arranged by size: 3478 1495 480 143 132 132 117 105 57 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6139 PvuII CAG'CTG Cuts at: 2120 2797 2806 3031 2120 Size: 677 9 225 5228 Fragments arranged by size: 5228 677 225 9 SacII CC_GC'GG Cuts at: 2847 3135 3179 2847 Size: 288 44 5807 Fragments arranged by size: 5807 288 44 SalI G'TCGA_C Cuts at: 1936 3481 1936 Size: 1545 4594 SapI GCTCTTCn'nnn_ Cuts at: 3086 3086 Size: 6139 SbfI CC_TGCA'GG Cuts at: 3491 3491 Size: 6139 ScaI AGT'ACT Cuts at: 4387 6099 4387 Size: 1712 4427 SexAI A'CCwGG_T Cuts at: 2667 3366 2667 Size: 699 5440 SfcI C'TryA_G Cuts at: 826 1045 2321 2453 2570 2675 2807 2864 3289 Size: 219 1276 132 117 105 132 57 425 Cuts at: 3289 3344 3487 4622 5303 5494 826 Size: 55 143 1135 681 191 1471 Fragments arranged by size: 1471 1276 1135 681 425 219 191 143 132 132 117 105 57 55 SfiI GGCCn_nnn'nGGCC Cuts at: 2603 3042 2603 Size: 439 5700 SfoI GGC'GCC Cuts at: 2623 2971 2623 Size: 348 5791 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6139 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6139 SmaI CCC'GGG Cuts at: 1872 3466 1872 Size: 1594 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4243 5144 5406 820 Size: 197 926 2300 901 262 1553 Fragments arranged by size: 2300 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3930 493 Size: 3437 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6139 SphI G_CATG'C Cuts at: 1132 3497 1132 Size: 2365 3774 SspI AAT'ATT Cuts at: 5 52 4063 5 Size: 47 4011 2081 Fragments arranged by size: 4011 2081 47 StuI AGG'CCT Cuts at: 1517 2711 2870 1517 Size: 1194 159 4786 Fragments arranged by size: 4786 1194 159 StyI C'CwwG_G Cuts at: 513 1065 2009 2064 3435 3578 513 Size: 552 944 55 1371 143 3074 Fragments arranged by size: 3074 1371 944 552 143 55 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2096 4166 4183 4336 4521 4966 325 Size: 1255 516 2070 17 153 185 445 1498 Fragments arranged by size: 2070 1498 1255 516 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2373 4385 6097 Size: 274 80 33 51 1443 396 2012 1712 Cuts at: 6097 96 Size: 138 Fragments arranged by size: 2012 1712 1443 396 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2784 2816 6099 6131 2784 Size: 32 3283 32 2792 Fragments arranged by size: 3283 2792 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6139 XbaI T'CTAG_A Cuts at: 3475 3475 Size: 6139 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2062 2062 Size: 6139 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6139 XmaI C'CCGG_G Cuts at: 1870 3464 1870 Size: 1594 4545 XmnI GAAnn'nnTTC Cuts at: 4268 4268 Size: 6139 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2920 3947 276 Size: 53 83 186 936 1386 1027 2468 Fragments arranged by size: 2468 1386 1027 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI Bsu36I EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SanDI SrfI SwaI