(Linear) (Six-base) MAPSORT of: KIEE4107.seq Check: 5116 from: 1 to: 1434 KIEE4107.orf With 182 enzymes: SgfI * March 22, 2012 17:22 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1391 1434 Size: 1391 43 AatII G_ACGT'C Cuts at: 0 917 1434 Size: 917 517 AccI GT'mk_AC Cuts at: 0 1406 1434 Size: 1406 28 Acc65I G'GTAC_C Cuts at: 0 362 515 1434 Size: 362 153 919 Fragments arranged by size: 919 362 153 AfeI AGC'GCT Cuts at: 0 154 1434 Size: 154 1280 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1156 1434 Size: 1156 278 AlwNI CAG_nnn'CTG Cuts at: 0 44 165 573 648 1434 Size: 44 121 408 75 786 Fragments arranged by size: 786 408 121 75 44 ApaI G_GGCC'C Cuts at: 0 502 1033 1434 Size: 502 531 401 Fragments arranged by size: 531 502 401 AscI GG'CGCG_CC Cuts at: 0 826 1249 1434 Size: 826 423 185 Fragments arranged by size: 826 423 185 AvaI C'yCGr_G Cuts at: 0 628 1032 1434 Size: 628 404 402 Fragments arranged by size: 628 404 402 AvrII C'CTAG_G Cuts at: 0 4 1434 Size: 4 1430 BanI G'GyrC_C Cuts at: 0 118 217 362 515 532 616 964 1434 Size: 118 99 145 153 17 84 348 470 Fragments arranged by size: 470 348 153 145 118 99 84 17 BanII G_rGCy'C Cuts at: 0 502 628 1033 1434 Size: 502 126 405 401 Fragments arranged by size: 502 405 401 126 BbeI G_GCGC'C Cuts at: 0 620 968 1434 Size: 620 348 466 Fragments arranged by size: 620 466 348 BbsI GAAGACnn'nnnn_ Cuts at: 0 336 1237 1434 Size: 336 901 197 Fragments arranged by size: 901 336 197 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 930 964 1434 Size: 930 34 470 Fragments arranged by size: 930 470 34 BglI GCCn_nnn'nGGC Cuts at: 0 598 748 1037 1089 1259 1434 Size: 598 150 289 52 170 175 Fragments arranged by size: 598 289 175 170 150 52 Bme1580I G_kGCm'C Cuts at: 0 220 502 535 1033 1135 1434 Size: 220 282 33 498 102 299 Fragments arranged by size: 498 299 282 220 102 33 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 133 304 703 721 1434 Size: 133 171 399 18 713 Fragments arranged by size: 713 399 171 133 18 BsaHI Gr'CG_yC Cuts at: 0 617 914 965 1124 1186 1434 Size: 617 297 51 159 62 248 Fragments arranged by size: 617 297 248 159 62 51 BsaWI w'CCGG_w Cuts at: 0 1421 1434 Size: 1421 13 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1321 1434 Size: 1321 113 BseYI C'CCAG_C Cuts at: 0 258 711 1026 1434 Size: 258 453 315 408 Fragments arranged by size: 453 408 315 258 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 226 250 397 877 1363 1369 1434 Size: 226 24 147 480 486 6 65 Fragments arranged by size: 486 480 226 147 65 24 6 BsiEI CG_ry'CG Cuts at: 0 593 727 1002 1167 1171 1434 Size: 593 134 275 165 4 263 Fragments arranged by size: 593 275 263 165 134 4 BsiHKAI G_wGCw'C Cuts at: 0 149 1076 1434 Size: 149 927 358 Fragments arranged by size: 927 358 149 Bsp1286I G_dGCh'C Cuts at: 0 149 220 502 535 628 1033 1076 1135 Size: 149 71 282 33 93 405 43 59 Cuts at: 1135 1434 Size: 299 Fragments arranged by size: 405 299 282 149 93 71 59 43 33 BspEI T'CCGG_A Cuts at: 0 1421 1434 Size: 1421 13 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1346 1391 1434 Size: 1346 45 43 Fragments arranged by size: 1346 45 43 BsrBI CCG'CTC Cuts at: 0 747 1434 Size: 747 687 BsrFI r'CCGG_y Cuts at: 0 783 1188 1312 1434 Size: 783 405 124 122 Fragments arranged by size: 783 405 124 122 BssHII G'CGCG_C Cuts at: 0 474 476 719 756 808 826 1150 1249 Size: 474 2 243 37 52 18 324 99 Cuts at: 1249 1434 Size: 185 Fragments arranged by size: 474 324 243 185 99 52 37 18 2 BstAPI GCAn_nnn'nTGC Cuts at: 0 165 648 1434 Size: 165 483 786 Fragments arranged by size: 786 483 165 BstEII G'GTnAC_C Cuts at: 0 72 1059 1434 Size: 72 987 375 Fragments arranged by size: 987 375 72 BstXI CCAn_nnnn'nTGG Cuts at: 0 192 1434 Size: 192 1242 BstZ17I GTA'TAC Cuts at: 0 1407 1434 Size: 1407 27 BtgI C'CryG_G Cuts at: 0 593 737 831 839 970 1127 1171 1434 Size: 593 144 94 8 131 157 44 263 Fragments arranged by size: 593 263 157 144 131 94 44 8 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 203 1434 Size: 203 1231 BtsI GCAGTG_nn' Cuts at: 0 1133 1434 Size: 1133 301 DraIII CAC_nnn'GTG Cuts at: 0 611 1434 Size: 611 823 EaeI y'GGCC_r Cuts at: 0 422 461 590 599 724 740 845 1038 Size: 422 39 129 9 125 16 105 193 Cuts at: 1038 1164 1190 1434 Size: 126 26 244 Fragments arranged by size: 422 244 193 129 126 125 105 39 26 16 9 EagI C'GGCC_G Cuts at: 0 590 724 1164 1434 Size: 590 134 440 270 Fragments arranged by size: 590 440 270 134 EarI CTCTTCn'nnn_ Cuts at: 0 1081 1434 Size: 1081 353 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1264 1434 Size: 1264 170 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 133 304 703 721 1434 Size: 133 171 399 18 713 Fragments arranged by size: 713 399 171 133 18 EcoNI CCTnn'n_nnAGG Cuts at: 0 8 859 1434 Size: 8 851 575 Fragments arranged by size: 851 575 8 EcoO109I rG'GnC_Cy Cuts at: 0 29 546 1434 Size: 29 517 888 Fragments arranged by size: 888 517 29 HaeII r_GCGC'y Cuts at: 0 156 620 754 929 968 1434 Size: 156 464 134 175 39 466 Fragments arranged by size: 466 464 175 156 134 39 HincII GTy'rAC Cuts at: 0 918 1434 Size: 918 516 KasI G'GCGC_C Cuts at: 0 616 964 1434 Size: 616 348 470 Fragments arranged by size: 616 470 348 KpnI G_GTAC'C Cuts at: 0 366 519 1434 Size: 366 153 915 Fragments arranged by size: 915 366 153 MscI TGG'CCA Cuts at: 0 424 1040 1434 Size: 424 616 394 Fragments arranged by size: 616 424 394 MspA1I CmG'CkG Cuts at: 0 115 340 792 801 841 891 1026 1129 Size: 115 225 452 9 40 50 135 103 Cuts at: 1129 1173 1199 1344 1434 Size: 44 26 145 90 Fragments arranged by size: 452 225 145 135 115 103 90 50 44 40 26 9 NaeI GCC'GGC Cuts at: 0 1190 1314 1434 Size: 1190 124 120 Fragments arranged by size: 1190 124 120 NarI GG'CG_CC Cuts at: 0 617 965 1434 Size: 617 348 469 Fragments arranged by size: 617 469 348 NgoMIV G'CCGG_C Cuts at: 0 1188 1312 1434 Size: 1188 124 122 Fragments arranged by size: 1188 124 122 NotI GC'GGCC_GC Cuts at: 0 724 1434 Size: 724 710 NspI r_CATG'y Cuts at: 0 474 1434 Size: 474 960 PflMI CCAn_nnn'nTGG Cuts at: 0 699 1434 Size: 699 735 PfoI T'CCnGG_A Cuts at: 0 281 1434 Size: 281 1153 PpuMI rG'GwC_Cy Cuts at: 0 29 1434 Size: 29 1405 PspOMI G'GGCC_C Cuts at: 0 498 1029 1434 Size: 498 531 405 Fragments arranged by size: 531 498 405 PstI C_TGCA'G Cuts at: 0 320 452 569 674 806 863 1343 1434 Size: 320 132 117 105 132 57 480 91 Fragments arranged by size: 480 320 132 132 117 105 91 57 PvuII CAG'CTG Cuts at: 0 115 792 801 1026 1434 Size: 115 677 9 225 408 Fragments arranged by size: 677 408 225 115 9 SacII CC_GC'GG Cuts at: 0 842 1130 1174 1434 Size: 842 288 44 260 Fragments arranged by size: 842 288 260 44 SapI GCTCTTCn'nnn_ Cuts at: 0 1081 1434 Size: 1081 353 SexAI A'CCwGG_T Cuts at: 0 662 1361 1434 Size: 662 699 73 Fragments arranged by size: 699 662 73 SfcI C'TryA_G Cuts at: 0 316 448 565 670 802 859 1284 1339 Size: 316 132 117 105 132 57 425 55 Cuts at: 1339 1434 Size: 95 Fragments arranged by size: 425 316 132 132 117 105 95 57 55 SfiI GGCCn_nnn'nGGCC Cuts at: 0 598 1037 1434 Size: 598 439 397 Fragments arranged by size: 598 439 397 SfoI GGC'GCC Cuts at: 0 618 966 1434 Size: 618 348 468 Fragments arranged by size: 618 468 348 StuI AGG'CCT Cuts at: 0 706 865 1434 Size: 706 159 569 Fragments arranged by size: 706 569 159 StyI C'CwwG_G Cuts at: 0 4 59 1434 Size: 4 55 1375 Fragments arranged by size: 1375 55 4 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 91 1434 Size: 91 1343 TatI w'GTAC_w Cuts at: 0 368 1434 Size: 368 1066 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 779 811 1434 Size: 779 32 623 Fragments arranged by size: 779 623 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 57 1434 Size: 57 1377 ZraI GAC'GTC Cuts at: 0 915 1434 Size: 915 519 Enzymes that do cut: AarI AatII AccI Acc65I AfeI AlfI AlwNI ApaI AscI AvaI AvrII BanI BanII BbeI BbsI BcgI BglI Bme1580I BpmI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspMI BsrBI BsrFI BssHII BstAPI BstEII BstXI BstZ17I BtgI BtgZI BtsI DraIII EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I HaeII HincII KasI KpnI MscI MspA1I NaeI NarI NgoMIV NotI NspI PflMI PfoI PpuMI PspOMI PstI PvuII SacII SapI SexAI SfcI SfiI SfoI StuI StyI TaqII TatI TstI XcmI ZraI Enzymes that do not cut: AclI AcuI AflII AflIII AgeI AhdI AleI AloI ApaLI ApoI AseI AsiSI BaeI BamHI BbvCI BciVI BclI BfrBI BglII BlpI BmgBI BmrI BmtI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaXI BsiWI BsmI BsmBI BspHI BsrDI BsrGI BssSI BstBI BstYI Bsu36I ClaI CspCI DraI DrdI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI Hin4I HindIII HpaI MfeI MluI MmeI MslI NcoI NdeI NheI NruI NsiI PacI PasI PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacI SalI SanDI SbfI ScaI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI SwaI Tth111I XbaI XhoI XmaI XmnI