Restriction map for
pFN21AE5648
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# NO CUT ENZYMES ( 125 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI ApoI
AscI Asp700I Asp718I AspEI AspI
AviII BalI BamHI BbeI BbrPI
BbvCI BcgI BclI BfrI BsaAI
BsaBI BsaI BsaWI BseAI BsgI
BsiEI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BsrBI BsrFI
BsrGI BssHII Bst1107I BstAPI BstEII
BstPI BstXI BstZ17I Bsu36I Cfr10I
ClaI CpoI DraI DraIII DsaI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
FbaI FseI FspI HpaI KasI
KpnI KspI MamI MfeI MluI
MluNI MroI MscI MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI PacI PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SgrAI SnaBI SpeI
Sse8387I SspBI SspI StuI SwaI
Tth111I Van91I XbaI XcmI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AcyI 385 GACGCC G[GA]^CG[CT]C
AfaI 1111 GTAC GT^AC
AflIII 938 ACATGT A^C[GA][CT]GT
AlwI 591 GGATC GGATC(4/5)
AseI 1353 ATTAAT AT^TAAT
AsnI 1353 ATTAAT AT^TAAT
AvrII 915 CCTAGG C^CTAGG
BanII 145 GAGCTC G[GA]GC[CT]^C
BbsI 699 GAAGAC GAAGAC(2/6)
BciVI 942 GTATCC GTATCC(6/5)
BglII 212 AGATCT A^GATCT
BlnI 915 CCTAGG C^CTAGG
BlpI 255 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 255 GCTTAGC GC^T[ACGT]AGC
BpuAI 699 GAAGAC GAAGAC(2/6)
BsaHI 385 GACGCC G[GA]^CG[CT]C
BseRI 728 CTCCTC GAGGAG(10/8)
BsmAI 1373 GAGAC GTCTC(1/5)
BspLU11I 938 ACATGT A^CATGT
BsrDI 950 GCAATG GCAATG(2/0)
BssSI 8 CTCGTG CACGAG(-5/-1)
BstBI 1287 TTCGAA TT^CGAA
CelII 255 GCTTAGC GC^T[ACGT]AGC
DrdI 1187 GACAGGTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT22I 1396 ATGCAT ATGCA^T
FauI 79 GCGGG CCCGC(4/6)
HaeII 201 GGCGCT [GA]GCGC^[CT]
HgaI 385 GACGC GACGC(5/10)
Hin1I 385 GACGCC G[GA]^CG[CT]C
HincII 96 GTTGAC GT[CT]^[GA]AC
HindII 96 GTTGAC GT[CT]^[GA]AC
HindIII 707 AAGCTT A^AGCTT
MslI 1377 CACCACAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 1063 CAGCTG C[AC]G^C[GT]G
NsiI 1396 ATGCAT ATGCA^T
NspV 1287 TTCGAA TT^CGAA
PaeR7I 148 CTCGAG C^TCGAG
PpuMI 1019 GGGACCT [GA]G^G[AT]CC[CT]
PshBI 1353 ATTAAT AT^TAAT
PvuII 1063 CAGCTG CAG^CTG
RsaI 1111 GTAC GT^AC
SacI 145 GAGCTC GAGCT^C
SfuI 1287 TTCGAA TT^CGAA
SphI 1034 GCATGC GCATG^C
Tsp45I 827 GTGAC ^GT[GC]AC
XhoI 148 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccII 141 CGCG CG^CG
460 CGCG
AlwNI 21 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG
825 CAGTGACTG
AspHI 145 GAGCTC G[AT]GC[AT]^C
163 GAGCAC
BglI 16 GCCGTCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
284 GCCACCCCGGC
BmyI 145 GAGCTC G[AGT]GC[ACT]^C
163 GAGCAC
BpmI 778 CTCCAG CTGGAG(16/14)
185 CTGGAG
BsiHKAI 145 GAGCTC G[AT]GC[AT]^C
163 GAGCAC
BsmFI 422 GGGAC GGGAC(10/14)
1019 GGGAC
Bsp1286I 145 GAGCTC G[AGT]GC[ACT]^C
163 GAGCAC
BspMI 41 GCAGGT ACCTGC(4/8)
369 GCAGGT
BsrI 747 CCAGT ACTGG(1/-1)
780 CCAGT
BstUI 141 CGCG CG^CG
460 CGCG
BstYI 212 AGATCT [GA]^GATC[CT]
591 GGATCT
CfoI 3 GCGC GCG^C
202 GCGC
EaeI 329 TGGCCG [CT]^GGCC[GA]
969 TGGCCG
HhaI 3 GCGC GCG^C
202 GCGC
HinP1I 3 GCGC G^CGC
202 GCGC
HphI 694 GGTGA GGTGA(8/7)
1122 TCACC
MaeII 518 ACGT A^CGT
768 ACGT
MflI 212 AGATCT [GA]^GATC[CT]
591 GGATCT
MvnI 141 CGCG CG^CG
460 CGCG
PleI 776 GACTC GAGTC(4/5)
804 GAGTC
PstI 39 CTGCAG CTGCA^G
446 CTGCAG
SfaNI 76 GATGC GCATC(5/9)
1395 GATGC
SfcI 39 CTGCAG C^T[GA][CT]AG
446 CTGCAG
SmaI 178 CCCGGG CCC^GGG
418 CCCGGG
SmlI 148 CTCGAG C^T[CT][GA]AG
511 CTTGAG
TfiI 265 GATTC G^A[AT]TC
1054 GATTC
XhoII 212 AGATCT [GA]^GATC[CT]
591 GGATCT
XmaCI 178 CCCGGG C^CCGGG
418 CCCGGG
XmaI 178 CCCGGG C^CCGGG
418 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AciI AluI AvaI AvaII BanI
BbvI BcnI BfaI BsaJI BsiYI
BslI BsoBI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII DraII
EarI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HinfI HpaII
ItaI Ksp632I MaeI MaeIII MboI
MboII MnlI MseI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI Sau3AI Sau96I ScrFI StyI
TaqI Tru9I TseI Tsp509I TspRI
TthHB8I