(Circular) (Six-base) MAPSORT of: pFN21AE5648.seq Check: 6603 from: 1 to: 6160 pFN21AE5648 6160 bp With 182 enzymes: SgfI * December 8, 2014 13:41 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2037 2037 Size: 6160 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3970 278 Size: 53 83 186 936 2434 2468 Fragments arranged by size: 2468 2434 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3503 1937 Size: 1566 4594 Acc65I G'GTAC_C Cuts at: 3481 3481 Size: 6160 AclI AA'CG_TT Cuts at: 1563 4287 4660 1563 Size: 2724 373 3063 Fragments arranged by size: 3063 2724 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2422 2730 2806 4223 5607 5875 1632 Size: 790 308 76 1417 1384 268 1917 Fragments arranged by size: 1917 1417 1384 790 308 268 76 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5963 AflIII A'CryG_T Cuts at: 1203 2943 5059 6036 1203 Size: 1740 2116 977 1327 Fragments arranged by size: 2116 1740 1327 977 AgeI A'CCGG_T Cuts at: 4991 4991 Size: 6160 AhdI GACnn_n'nnGTC Cuts at: 4889 4889 Size: 6160 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6160 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6160 AlwNI CAG_nnn'CTG Cuts at: 2031 2835 3903 5475 2031 Size: 804 1068 1572 2716 Fragments arranged by size: 2716 1572 1068 804 ApaLI G'TGCA_C Cuts at: 4217 5373 4217 Size: 1156 5004 ApoI r'AATT_y Cuts at: 1493 3469 3695 3847 5956 1493 Size: 1976 226 152 2109 1697 Fragments arranged by size: 2109 1976 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 3359 4714 160 Size: 872 2327 1355 1606 Fragments arranged by size: 2327 1606 1355 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6160 AvaI C'yCGr_G Cuts at: 1870 1943 2153 2183 2423 3485 1870 Size: 73 210 30 240 1062 4545 Fragments arranged by size: 4545 1062 240 210 73 30 AvrII C'CTAG_G Cuts at: 2920 2920 Size: 6160 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2930 2963 2930 Size: 33 6127 BamHI G'GATC_C Cuts at: 3490 3857 3490 Size: 367 5793 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2049 2286 2567 3481 4936 Size: 325 217 627 262 237 281 914 1455 Cuts at: 4936 618 Size: 1842 Fragments arranged by size: 1842 1455 914 627 325 281 262 237 217 BanII G_rGCy'C Cuts at: 1391 2154 3479 1391 Size: 763 1325 4072 Fragments arranged by size: 4072 1325 763 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2711 928 Size: 971 812 4377 Fragments arranged by size: 4377 971 812 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4351 4385 1903 Size: 34 50 34 2330 34 3678 Fragments arranged by size: 3678 2330 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2958 4052 5262 2958 Size: 1094 1210 3856 Fragments arranged by size: 3856 1210 1094 BclI T'GATC_A Cuts at: 1548 1548 Size: 6160 BfrBI ATG'CAT Cuts at: 3403 3403 Size: 6160 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2027 2295 4771 Size: 107 122 71 631 605 355 268 2476 Cuts at: 4771 136 Size: 1525 Fragments arranged by size: 2476 1525 631 605 355 268 122 107 71 BglII A'GATC_T Cuts at: 2217 6155 2217 Size: 3938 2222 BlpI GC'TnA_GC Cuts at: 2261 3577 2261 Size: 1316 4844 Bme1580I G_kGCm'C Cuts at: 1790 4221 5377 1790 Size: 2431 1156 2573 Fragments arranged by size: 2573 2431 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6160 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3891 4849 442 Size: 416 53 83 399 24 2474 958 1753 Fragments arranged by size: 2474 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6160 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2211 2768 4820 1239 Size: 972 557 2052 2579 Fragments arranged by size: 2579 2052 972 557 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2537 4285 5150 5448 2537 Size: 1748 865 298 3249 Fragments arranged by size: 3249 1748 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4823 882 Size: 617 3324 2219 Fragments arranged by size: 3324 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3951 493 Size: 711 2747 2702 Fragments arranged by size: 2747 2702 711 BsaBI GATnn'nnATC Cuts at: 6154 6154 Size: 6160 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2391 3967 4349 4984 Size: 53 83 186 936 858 1576 382 635 Cuts at: 4984 275 Size: 1451 Fragments arranged by size: 1576 1451 936 858 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4593 4991 5265 5412 5823 4593 Size: 398 274 147 411 4930 Fragments arranged by size: 4930 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2118 2148 1178 Size: 30 165 30 715 30 5190 Fragments arranged by size: 5190 715 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2724 1186 Size: 1538 4622 BseYI C'CCAG_C Cuts at: 5363 5859 5363 Size: 496 5664 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5587 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3610 4371 5399 1540 Size: 275 186 1609 761 1028 2301 Fragments arranged by size: 2301 1609 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2154 2172 4221 4306 5377 2154 Size: 18 2049 85 1071 2937 Fragments arranged by size: 2937 2049 1071 85 18 BsmI GAATG_Cn' Cuts at: 3671 3764 3671 Size: 93 6067 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2154 2172 3479 4221 4306 5377 1391 Size: 399 364 18 1307 742 85 1071 2174 Fragments arranged by size: 2174 1307 1071 742 399 364 85 18 BspEI T'CCGG_A Cuts at: 5823 5823 Size: 6160 BspHI T'CATG_A Cuts at: 4049 5779 4049 Size: 1730 4430 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2037 2365 3515 844 Size: 1193 328 1150 3489 Fragments arranged by size: 3489 1193 1150 328 BsrBI CCG'CTC Cuts at: 1394 1823 4047 1394 Size: 429 2224 3507 Fragments arranged by size: 3507 2224 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2962 4655 4829 65 Size: 1183 1714 1693 174 1396 Fragments arranged by size: 1714 1693 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4804 4991 1700 Size: 110 2994 187 2869 Fragments arranged by size: 2994 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6160 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6160 BssSI C'ACGA_G Cuts at: 2013 4220 5232 2013 Size: 2207 1012 2941 Fragments arranged by size: 2941 2207 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6160 BstBI TT'CG_AA Cuts at: 3293 4964 3293 Size: 1671 4489 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6160 BstYI r'GATC_y Cuts at: 1972 2217 2596 3490 3857 4242 4259 5711 5797 Size: 245 379 894 367 385 17 1452 86 Cuts at: 5797 5809 6155 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 894 385 379 367 346 245 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5608 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5421 Fragments arranged by size: 5421 683 56 BtsI GCAGTG_nn' Cuts at: 2836 3676 4470 4490 2836 Size: 840 794 20 4506 Fragments arranged by size: 4506 840 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6160 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6125 DraI TTT'AAA Cuts at: 3464 3464 Size: 6160 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3198 5167 809 Size: 507 1882 1969 1802 Fragments arranged by size: 1969 1882 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2334 2974 3607 Size: 54 1425 50 275 18 504 640 633 Cuts at: 3607 4496 6125 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 640 633 504 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3607 1537 Size: 275 1795 4090 Fragments arranged by size: 4090 1795 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2280 3163 3323 4090 1353 Size: 927 883 160 767 3423 Fragments arranged by size: 3423 927 883 767 160 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4747 5121 5267 387 Size: 1064 138 3158 374 146 1280 Fragments arranged by size: 3158 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2152 2152 Size: 6160 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2211 2422 2730 2768 2806 4223 4820 Size: 393 579 211 308 38 38 1417 597 Cuts at: 4820 5607 5875 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1417 787 597 579 393 308 268 211 38 38 EcoO109I rG'GnC_Cy Cuts at: 2028 2411 3025 3173 2028 Size: 383 614 148 5015 Fragments arranged by size: 5015 614 383 148 EcoRI G'AATT_C Cuts at: 3469 3847 3469 Size: 378 5782 FspI TGC'GCA Cuts at: 4666 4666 Size: 6160 HaeII r_GCGC'y Cuts at: 1678 2210 5307 1678 Size: 532 3097 2531 Fragments arranged by size: 3097 2531 532 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4801 4833 4875 4907 1373 Size: 32 135 32 3229 32 42 32 2626 Fragments arranged by size: 3229 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2103 3504 3750 669 Size: 988 281 165 1401 246 3079 Fragments arranged by size: 3079 1401 988 281 246 165 HindIII A'AGCT_T Cuts at: 748 2712 748 Size: 1964 4196 HpaI GTT'AAC Cuts at: 3750 3750 Size: 6160 KpnI G_GTAC'C Cuts at: 3485 3485 Size: 6160 MfeI C'AATT_G Cuts at: 3759 3759 Size: 6160 MluI A'CGCG_T Cuts at: 6036 6036 Size: 6160 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5274 5458 1305 Size: 3969 184 2007 Fragments arranged by size: 3969 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4338 Fragments arranged by size: 4338 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3386 4118 4477 4636 518 Size: 648 2220 732 359 159 2042 Fragments arranged by size: 2220 2042 732 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3070 3576 4253 5401 5646 1606 Size: 1464 506 677 1148 245 2120 Fragments arranged by size: 2120 1464 1148 677 506 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5608 NdeI CA'TA_TG Cuts at: 387 387 Size: 6160 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6160 NotI GC'GGCC_GC Cuts at: 3607 3607 Size: 6160 NsiI A_TGCA'T Cuts at: 3405 3405 Size: 6160 NspI r_CATG'y Cuts at: 1132 1229 2135 2947 3043 3518 5063 1132 Size: 97 906 812 96 475 1545 2229 Fragments arranged by size: 2229 1545 906 812 475 97 96 PasI CC'CwG_GG Cuts at: 1348 2886 1348 Size: 1538 4622 PciI A'CATG_T Cuts at: 2943 5059 2943 Size: 2116 4044 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6160 PmeI GTTT'AAAC Cuts at: 3464 3464 Size: 6160 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4229 4261 5742 5774 4229 Size: 32 1481 32 4615 Fragments arranged by size: 4615 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3025 3025 Size: 6160 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6160 PsiI TTA'TAA Cuts at: 3730 3730 Size: 6160 PspXI vC'TCGA_Gb Cuts at: 2153 2153 Size: 6160 PstI C_TGCA'G Cuts at: 830 2048 2455 3512 830 Size: 1218 407 1057 3478 Fragments arranged by size: 3478 1218 1057 407 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6160 PvuII CAG'CTG Cuts at: 3070 3070 Size: 6160 SacI G_AGCT'C Cuts at: 2154 2154 Size: 6160 SalI G'TCGA_C Cuts at: 1936 3502 1936 Size: 1566 4594 SbfI CC_TGCA'GG Cuts at: 3512 3512 Size: 6160 ScaI AGT'ACT Cuts at: 4408 6120 4408 Size: 1712 4448 SfcI C'TryA_G Cuts at: 826 1045 2044 2451 3508 4643 5324 5515 826 Size: 219 999 407 1057 1135 681 191 1471 Fragments arranged by size: 1471 1135 1057 999 681 407 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6160 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6160 SmaI CCC'GGG Cuts at: 1872 2185 2425 3487 1872 Size: 313 240 1062 4545 Fragments arranged by size: 4545 1062 313 240 SmlI C'TyrA_G Cuts at: 820 1017 1943 2153 2516 4264 5165 5427 820 Size: 197 926 210 363 1748 901 262 1553 Fragments arranged by size: 1748 1553 926 901 363 262 210 197 SnaBI TAC'GTA Cuts at: 493 3951 493 Size: 3458 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6160 SphI G_CATG'C Cuts at: 1132 3043 3518 1132 Size: 1911 475 3774 Fragments arranged by size: 3774 1911 475 SspI AAT'ATT Cuts at: 5 52 4084 5 Size: 47 4032 2081 Fragments arranged by size: 4032 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6160 StyI C'CwwG_G Cuts at: 513 1065 2307 2571 2920 3599 513 Size: 552 1242 264 349 679 3074 Fragments arranged by size: 3074 1242 679 552 349 264 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2187 2274 3048 4187 4204 4357 4542 Size: 1255 607 87 774 1139 17 153 185 Cuts at: 4542 4987 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1139 774 607 445 185 153 87 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4406 6118 96 Size: 274 80 33 51 1443 2429 1712 138 Fragments arranged by size: 2429 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2118 2150 6120 6152 2118 Size: 32 3970 32 2126 Fragments arranged by size: 3970 2126 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6160 XbaI T'CTAG_A Cuts at: 3496 3496 Size: 6160 XhoI C'TCGA_G Cuts at: 1943 2153 1943 Size: 210 5950 XmaI C'CCGG_G Cuts at: 1870 2183 2423 3485 1870 Size: 313 240 1062 4545 Fragments arranged by size: 4545 1062 313 240 XmnI GAAnn'nnTTC Cuts at: 4289 4289 Size: 6160 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3968 276 Size: 53 83 186 936 2434 2468 Fragments arranged by size: 2468 2434 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI