################################## # NO CUT ENZYMES ( 85 enzymes) # ################################## AatII AccI AccII AclI AgeI Alw44I ApaI ApaLI AscI AseI AsnI AspI AvrII BbeI BcgI BclI BglI BlnI BsiEI BsiWI BsmBI Bsp1407I BspDI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstZ17I Cfr10I ClaI CpoI EagI EclXI Eco52I EcoO65I EcoT22I FbaI FseI HpaI KasI KspI MluI MvnI NaeI NarI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmeI PshBI Psp1406I PvuI RsrII SacII SalI SbfI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SwaI Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### Acc65I 3936 GGTACC G^GTACC AccIII 1110 TCCGGA T^CCGGA AcyI 4327 GACGCC G[GA]^CG[CT]C AflII 3844 CTTAAG C^TTAAG AflIII 2683 ACATGT A^C[GA][CT]GT AhdI 4774 GACTTTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Aor51HI 4096 AGCGCT AGC^GCT Asp718I 3936 GGTACC G^GTACC AspEI 4774 GACTTTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaI 3905 CTCGGG C^[CT]CG[GA]G BalI 4403 TGGCCA TGG^CCA BamHI 3597 GGATCC G^GATCC BbrPI 361 CACGTG CAC^GTG BciVI 3376 GTATCC GTATCC(6/5) BfrI 3844 CTTAAG C^TTAAG BlpI 3788 GCTTAGC GC^T[ACGT]AGC Bpu1102I 3788 GCTTAGC GC^T[ACGT]AGC BsaHI 4327 GACGCC G[GA]^CG[CT]C BsaI 3944 GAGACC GGTCTC(1/5) BsaWI 1110 TCCGGA [AT]^CCGG[AT] BseAI 1110 TCCGGA T^CCGGA BsoBI 3905 CTCGGG C^[CT]CG[GA]G BspEI 1110 TCCGGA T^CCGGA BspLU11I 2683 ACATGT A^CATGT BstAPI 2434 GCAAAATATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC CelII 3788 GCTTAGC GC^T[ACGT]AGC DraIII 361 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 1836 GACACAGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 4403 TGGCCA [CT]^GGCC[GA] Eam1105I 4774 GACTTTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 4096 AGCGCT AGC^GCT EcoRI 4559 GAATTC G^AATTC EcoRV 756 GATATC GAT^ATC HgaI 4327 GACGC GACGC(5/10) Hin1I 4327 GACGCC G[GA]^CG[CT]C HincII 832 GTTGAC GT[CT]^[GA]AC HindII 832 GTTGAC GT[CT]^[GA]AC HindIII 2416 AAGCTT A^AGCTT KpnI 3936 GGTACC GGTAC^C MfeI 2630 CAATTG C^AATTG MluNI 4403 TGGCCA TGG^CCA MroI 1110 TCCGGA T^CCGGA MscI 4403 TGGCCA TGG^CCA MunI 2630 CAATTG C^AATTG PmaCI 361 CACGTG CAC^GTG PmlI 361 CACGTG CAC^GTG PshAI 3706 GACAGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacI 3991 GAGCTC GAGCT^C SapI 3736 GAAGAGC GCTCTTC(1/4) ScaI 4796 AGTACT AGT^ACT SexAI 2090 ACCTGGT A^CC[AT]GGT StuI 2597 AGGCCT AGG^CCT ################################### # TWO CUT ENZYMES ( 12 enzymes) # ################################### AviII 1976 TGCGCA TGC^GCA 4038 TGCGCA BsaAI 361 CACGTG [CT]AC^GT[GA] 1357 TACGTG BspHI 2231 TCATGA T^CATGA 2511 TCATGA BspMI 1157 GCAGGT ACCTGC(4/8) 1323 GCAGGT DraI 1893 TTTAAA TTT^AAA 2844 TTTAAA EcoNI 776 CCTGTTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1065 CCTTTTCCAGG FauI 1796 CCCGC CCCGC(4/6) 1899 GCGGG FspI 1976 TGCGCA TGC^GCA 4038 TGCGCA HaeII 45 GGCGCT [GA]GCGC^[CT] 4096 AGCGCT NcoI 2649 CCATGG C^CATGG 3511 CCATGG PstI 1304 CTGCAG CTGCA^G 2591 CTGCAG RcaI 2231 TCATGA T^CATGA 2511 TCATGA ######################################### # 3 OR MORE CUT ENZYMES ( 109 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI AocI ApoI Asp700I AspHI AvaII BanI BanII BbsI BbvCI BbvI BcnI BfaI BglII BmyI BpmI BpuAI BsaBI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BsrDI BsrI BssKI BstF5I BstNI BstXI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EarI Eco81I EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MamI MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII XmnI