(Circular) (Six-base) MAPSORT of: pFN21AE3477.seq Check: 7347 from: 1 to: 9517 pFN21AE3477 9517 bp With 182 enzymes: SgfI * July 6, 2012 17:43 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 7327 278 Size: 53 83 186 936 5791 2468 Fragments arranged by size: 5791 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 6860 1937 Size: 4923 4594 Acc65I G'GTAC_C Cuts at: 5941 6838 5941 Size: 897 8620 AclI AA'CG_TT Cuts at: 1563 7644 8017 1563 Size: 6081 373 3063 Fragments arranged by size: 6081 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2108 3432 4580 7580 8964 9232 1632 Size: 476 1324 1148 3000 1384 268 1917 Fragments arranged by size: 3000 1917 1384 1324 1148 476 268 AfeI AGC'GCT Cuts at: 6103 6103 Size: 9517 AflII C'TTAA_G Cuts at: 820 1017 5849 820 Size: 197 4832 4488 Fragments arranged by size: 4832 4488 197 AflIII A'CryG_T Cuts at: 1203 4688 8416 9393 1203 Size: 3485 3728 977 1327 Fragments arranged by size: 3728 3485 1327 977 AgeI A'CCGG_T Cuts at: 8348 8348 Size: 9517 AhdI GACnn_n'nnGTC Cuts at: 6784 8246 6784 Size: 1462 8055 AleI CACnn'nnGTG Cuts at: 1166 5141 1166 Size: 3975 5542 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3487 6568 1184 Size: 2303 3081 4133 Fragments arranged by size: 4133 3081 2303 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5256 5288 6148 6180 5256 Size: 32 860 32 8593 Fragments arranged by size: 8593 860 32 32 AlwNI CAG_nnn'CTG Cuts at: 3425 3698 4276 5538 6639 7260 8832 3425 Size: 273 578 1262 1101 621 1572 4110 Fragments arranged by size: 4110 1572 1262 1101 621 578 273 ApaLI G'TGCA_C Cuts at: 7574 8730 7574 Size: 1156 8361 ApoI r'AATT_y Cuts at: 1493 2813 3383 3954 4992 5903 6564 6826 7052 Size: 1320 570 571 1038 911 661 262 226 Cuts at: 7052 7204 9313 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 1320 1038 911 661 571 570 262 226 152 AseI AT'TA_AT Cuts at: 160 1032 8071 160 Size: 872 7039 1606 Fragments arranged by size: 7039 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9517 AvaI C'yCGr_G Cuts at: 1870 1943 5910 6842 1870 Size: 73 3967 932 4545 Fragments arranged by size: 4545 3967 932 73 BamHI G'GATC_C Cuts at: 5602 6847 7214 5602 Size: 1245 367 7905 Fragments arranged by size: 7905 1245 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2009 5511 5941 6838 8293 Size: 325 217 627 222 3502 430 897 1455 Cuts at: 8293 618 Size: 1842 Fragments arranged by size: 3502 1842 1455 897 627 430 325 222 217 BanII G_rGCy'C Cuts at: 1391 5399 5700 6000 6009 6836 1391 Size: 4008 301 300 9 827 4072 Fragments arranged by size: 4072 4008 827 301 300 9 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3021 4505 5565 6492 6783 928 Size: 971 1122 1484 1060 927 291 3662 Fragments arranged by size: 3662 1484 1122 1060 971 927 291 BbvCI CC'TCA_GC Cuts at: 2036 2054 2187 2036 Size: 18 133 9366 Fragments arranged by size: 9366 133 18 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 7708 7742 1903 Size: 34 5771 34 3678 Fragments arranged by size: 5771 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5392 7409 8619 5392 Size: 2017 1210 6290 Fragments arranged by size: 6290 2017 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 9517 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 8128 136 Size: 107 122 71 631 605 6456 1525 Fragments arranged by size: 6456 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3024 4522 4667 9512 3024 Size: 1498 145 4845 3029 Fragments arranged by size: 4845 3029 1498 145 BlpI GC'TnA_GC Cuts at: 5794 6934 5794 Size: 1140 8377 Bme1580I G_kGCm'C Cuts at: 1790 5516 7578 8734 1790 Size: 3726 2062 1156 2573 Fragments arranged by size: 3726 2573 2062 1156 BmgBI CAC'GTC Cuts at: 1318 6070 1318 Size: 4752 4765 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4594 7248 8206 Size: 416 53 83 399 24 3177 2654 958 Cuts at: 8206 442 Size: 1753 Fragments arranged by size: 3177 2654 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 9517 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4413 4413 Size: 9517 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2520 2583 2842 3049 5839 6154 8177 1239 Size: 1281 63 259 207 2790 315 2023 2579 Fragments arranged by size: 2790 2579 2023 1281 315 259 207 63 Bpu10I CC'TnA_GC Cuts at: 2036 2054 2187 6077 2036 Size: 18 133 3890 5476 Fragments arranged by size: 5476 3890 133 18 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2621 3582 4228 4656 6358 7642 8507 8805 2621 Size: 961 646 428 1702 1284 865 298 3333 Fragments arranged by size: 3333 1702 1284 961 865 646 428 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5943 8180 882 Size: 617 4444 2237 2219 Fragments arranged by size: 4444 2237 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2368 3364 7308 493 Size: 711 1164 996 3944 2702 Fragments arranged by size: 3944 2702 1164 996 711 BsaBI GATnn'nnATC Cuts at: 2730 4053 4527 5601 9511 2730 Size: 1323 474 1074 3910 2736 Fragments arranged by size: 3910 2736 1323 1074 474 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 6333 7324 7706 8341 Size: 53 83 186 936 4800 991 382 635 Cuts at: 8341 275 Size: 1451 Fragments arranged by size: 4800 1451 991 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3115 7950 8348 8622 8769 9180 3115 Size: 4835 398 274 147 411 3452 Fragments arranged by size: 4835 3452 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2244 2274 1178 Size: 30 165 30 841 30 8421 Fragments arranged by size: 8421 841 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2060 2071 4151 5038 6229 6375 1186 Size: 874 11 2080 887 1191 146 4328 Fragments arranged by size: 4328 2080 1191 887 874 146 11 BseYI C'CCAG_C Cuts at: 3520 5297 6056 6308 6355 8720 9216 3520 Size: 1777 759 252 47 2365 496 3821 Fragments arranged by size: 3821 2365 1777 759 496 252 47 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2213 4397 5504 1163 Size: 573 477 2184 1107 5176 Fragments arranged by size: 5176 2184 1107 573 477 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6967 7728 8756 1540 Size: 275 186 4966 761 1028 2301 Fragments arranged by size: 4966 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3712 6000 6075 7578 7663 8734 3712 Size: 2288 75 1503 85 1071 4495 Fragments arranged by size: 4495 2288 1503 1071 85 75 BsmI GAATG_Cn' Cuts at: 2364 2980 3895 3942 5133 5316 7028 7121 2364 Size: 616 915 47 1191 183 1712 93 4760 Fragments arranged by size: 4760 1712 1191 915 616 183 93 47 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3712 5399 5516 5700 6000 6009 6075 Size: 399 1922 1687 117 184 300 9 66 Cuts at: 6075 6836 7578 7663 8734 1391 Size: 761 742 85 1071 2174 Fragments arranged by size: 2174 1922 1687 1071 761 742 399 300 184 117 85 66 9 BspEI T'CCGG_A Cuts at: 3115 9180 3115 Size: 6065 3452 BspHI T'CATG_A Cuts at: 4236 4516 7406 9136 4236 Size: 280 2890 1730 4617 Fragments arranged by size: 4617 2890 1730 280 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3153 3319 6872 844 Size: 2309 166 3553 3489 Fragments arranged by size: 3553 3489 2309 166 BsrBI CCG'CTC Cuts at: 1394 1823 7404 1394 Size: 429 5581 3507 Fragments arranged by size: 5581 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5232 6451 6463 8012 8186 65 Size: 1183 3984 1219 12 1549 174 1396 Fragments arranged by size: 3984 1549 1396 1219 1183 174 12 BsrFI r'CCGG_y Cuts at: 1700 1810 8161 8348 1700 Size: 110 6351 187 2869 Fragments arranged by size: 6351 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 9517 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9517 BssSI C'ACGA_G Cuts at: 7577 8589 7577 Size: 1012 8505 BstAPI GCAn_nnn'nTGC Cuts at: 794 4445 794 Size: 3651 5866 BstBI TT'CG_AA Cuts at: 8321 8321 Size: 9517 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 9517 BstXI CCAn_nnnn'nTGG Cuts at: 2995 3256 5707 6016 2995 Size: 261 2451 309 6496 Fragments arranged by size: 6496 2451 309 261 BstYI r'GATC_y Cuts at: 1972 2944 3024 4522 4667 5602 5812 6847 7214 Size: 972 80 1498 145 935 210 1035 367 Cuts at: 7214 7599 7616 9068 9154 9166 9512 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1498 1452 1035 972 935 385 367 346 210 145 86 80 17 12 Bsu36I CC'TnA_GG Cuts at: 4134 4938 5409 6387 4134 Size: 804 471 978 7264 Fragments arranged by size: 7264 978 804 471 BtgI C'CryG_G Cuts at: 513 1065 2004 4654 5516 6200 513 Size: 552 939 2650 862 684 3830 Fragments arranged by size: 3830 2650 939 862 684 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 8778 Fragments arranged by size: 8778 683 56 BtsI GCAGTG_nn' Cuts at: 2137 3215 5788 7033 7827 7847 2137 Size: 1078 2573 1245 794 20 3807 Fragments arranged by size: 3807 2573 1245 1078 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 9517 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4623 4658 4672 4707 6189 6224 529 Size: 35 4059 35 14 35 1482 35 3822 Fragments arranged by size: 4059 3822 1482 35 35 35 35 14 DraI TTT'AAA Cuts at: 3900 4851 6821 3900 Size: 951 1970 6596 Fragments arranged by size: 6596 1970 951 DraIII CAC_nnn'GTG Cuts at: 2371 2371 Size: 9517 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3847 8524 809 Size: 507 2531 4677 1802 Fragments arranged by size: 4677 2531 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 6408 6964 7853 Size: 54 1425 50 275 18 4578 556 889 Cuts at: 7853 9482 8 Size: 1629 43 Fragments arranged by size: 4578 1629 1425 889 556 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6964 1537 Size: 275 5152 4090 Fragments arranged by size: 5152 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2277 3372 3667 3818 4044 5056 5208 5736 Size: 924 1095 295 151 226 1012 152 528 Cuts at: 5736 6057 7447 1353 Size: 321 1390 3423 Fragments arranged by size: 3423 1390 1095 1012 924 528 321 295 226 152 151 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 8104 8478 8624 387 Size: 1064 138 6515 374 146 1280 Fragments arranged by size: 6515 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 5998 5998 Size: 9517 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2108 2520 2583 2842 3049 3432 4580 Size: 393 476 412 63 259 207 383 1148 Cuts at: 4580 5839 6154 7580 8177 8964 9232 1239 Size: 1259 315 1426 597 787 268 1524 Fragments arranged by size: 1524 1426 1259 1148 787 597 476 412 393 383 315 268 259 207 63 EcoNI CCTnn'n_nnAGG Cuts at: 2785 3074 2785 Size: 289 9228 EcoO109I rG'GnC_Cy Cuts at: 2106 2792 5095 2106 Size: 686 2303 6528 Fragments arranged by size: 6528 2303 686 EcoRI G'AATT_C Cuts at: 6564 6826 7204 6564 Size: 262 378 8877 Fragments arranged by size: 8877 378 262 EcoRV GAT'ATC Cuts at: 2763 2763 Size: 9517 FspI TGC'GCA Cuts at: 3983 6045 8023 3983 Size: 2062 1978 5477 Fragments arranged by size: 5477 2062 1978 HaeII r_GCGC'y Cuts at: 1678 2054 6105 8664 1678 Size: 376 4051 2559 2531 Fragments arranged by size: 4051 2559 2531 376 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2244 2276 2986 3018 3257 Size: 32 135 32 672 32 710 32 239 Cuts at: 3257 3289 3741 3773 4127 4159 5033 5065 5483 Size: 32 452 32 354 32 874 32 418 Cuts at: 5483 5515 5984 6016 6659 6691 6770 6802 8158 Size: 32 469 32 643 32 79 32 1356 Cuts at: 8158 8190 8232 8264 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1356 874 710 672 643 469 452 418 354 239 135 79 42 32 32 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2839 6861 7107 669 Size: 988 281 901 4022 246 3079 Fragments arranged by size: 4022 3079 988 901 281 246 HindIII A'AGCT_T Cuts at: 748 4421 748 Size: 3673 5844 HpaI GTT'AAC Cuts at: 7107 7107 Size: 9517 KpnI G_GTAC'C Cuts at: 5945 6842 5945 Size: 897 8620 MfeI C'AATT_G Cuts at: 4635 7116 4635 Size: 2481 7036 MluI A'CGCG_T Cuts at: 9393 9393 Size: 9517 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2230 4944 5054 8631 8815 1305 Size: 925 2714 110 3577 184 2007 Fragments arranged by size: 3577 2714 2007 925 184 110 MscI TGG'CCA Cuts at: 10 64 1489 1832 6410 10 Size: 54 1425 343 4578 3117 Fragments arranged by size: 4578 3117 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2993 3203 3723 3860 4308 4337 4701 Size: 648 1827 210 520 137 448 29 364 Cuts at: 4701 5141 5664 6657 7475 7834 7993 518 Size: 440 523 993 818 359 159 2042 Fragments arranged by size: 2042 1827 993 818 648 523 520 448 440 364 359 210 159 137 29 MspA1I CmG'CkG Cuts at: 1606 3435 3661 4861 5715 6162 6639 6933 7610 Size: 1829 226 1200 854 447 477 294 677 Cuts at: 7610 8758 9003 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1829 1200 1148 854 677 477 447 294 245 226 NcoI C'CATG_G Cuts at: 513 1065 2004 4654 5516 513 Size: 552 939 2650 862 4514 Fragments arranged by size: 4514 2650 939 862 552 NdeI CA'TA_TG Cuts at: 387 2940 4350 4549 387 Size: 2553 1410 199 5355 Fragments arranged by size: 5355 2553 1410 199 NheI G'CTAG_C Cuts at: 1052 1052 Size: 9517 NotI GC'GGCC_GC Cuts at: 6964 6964 Size: 9517 NspI r_CATG'y Cuts at: 1132 1229 4233 4692 5197 6353 6441 6875 8420 Size: 97 3004 459 505 1156 88 434 1545 Cuts at: 8420 1132 Size: 2229 Fragments arranged by size: 3004 2229 1545 1156 505 459 434 97 88 PasI CC'CwG_GG Cuts at: 1348 5986 1348 Size: 4638 4879 PciI A'CATG_T Cuts at: 4688 8416 4688 Size: 3728 5789 PflMI CCAn_nnn'nTGG Cuts at: 1108 2371 2557 3210 3322 1108 Size: 1263 186 653 112 7303 Fragments arranged by size: 7303 1263 653 186 112 PfoI T'CCnGG_A Cuts at: 2148 2633 4080 2148 Size: 485 1447 7585 Fragments arranged by size: 7585 1447 485 PmeI GTTT'AAAC Cuts at: 6821 6821 Size: 9517 PmlI CAC'GTG Cuts at: 2368 2368 Size: 9517 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 7586 7618 9099 9131 7586 Size: 32 1481 32 7972 Fragments arranged by size: 7972 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2106 2792 5095 2106 Size: 686 2303 6528 Fragments arranged by size: 6528 2303 686 PshAI GACnn'nnGTC Cuts at: 1613 5715 1613 Size: 4102 5415 PsiI TTA'TAA Cuts at: 6534 7087 6534 Size: 553 8964 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 4092 4124 4092 Size: 32 9485 PstI C_TGCA'G Cuts at: 830 3313 4600 6869 830 Size: 2483 1287 2269 3478 Fragments arranged by size: 3478 2483 2269 1287 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9517 PvuII CAG'CTG Cuts at: 3661 4861 5715 6162 6639 3661 Size: 1200 854 447 477 6539 Fragments arranged by size: 6539 1200 854 477 447 SacI G_AGCT'C Cuts at: 6000 6000 Size: 9517 SalI G'TCGA_C Cuts at: 1936 6859 1936 Size: 4923 4594 SanDI GG'GwC_CC Cuts at: 2792 2792 Size: 9517 SapI GCTCTTCn'nnn_ Cuts at: 5736 5736 Size: 9517 SbfI CC_TGCA'GG Cuts at: 6869 6869 Size: 9517 ScaI AGT'ACT Cuts at: 6803 7765 9477 6803 Size: 962 1712 6843 Fragments arranged by size: 6843 1712 962 SexAI A'CCwGG_T Cuts at: 4095 4095 Size: 9517 SfcI C'TryA_G Cuts at: 826 1045 3309 3372 4596 6451 6865 8000 8681 Size: 219 2264 63 1224 1855 414 1135 681 Cuts at: 8681 8872 826 Size: 191 1471 Fragments arranged by size: 2264 1855 1471 1224 1135 681 414 219 191 63 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9517 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9517 SmaI CCC'GGG Cuts at: 1872 6844 1872 Size: 4972 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2600 3597 4243 4671 5849 6337 Size: 197 926 657 997 646 428 1178 488 Cuts at: 6337 7621 8522 8784 820 Size: 1284 901 262 1553 Fragments arranged by size: 1553 1284 1178 997 926 901 657 646 488 428 262 197 SnaBI TAC'GTA Cuts at: 493 7308 493 Size: 6815 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 9517 SphI G_CATG'C Cuts at: 1132 6875 1132 Size: 5743 3774 SspI AAT'ATT Cuts at: 5 52 3538 4070 5112 7441 5 Size: 47 3486 532 1042 2329 2081 Fragments arranged by size: 3486 2329 2081 1042 532 47 StuI AGG'CCT Cuts at: 1517 4604 1517 Size: 3087 6430 StyI C'CwwG_G Cuts at: 513 1065 2004 2013 4654 5516 6956 513 Size: 552 939 9 2641 862 1440 3074 Fragments arranged by size: 3074 2641 1440 939 862 552 9 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 1997 3850 5845 6560 7544 7561 7714 Size: 1255 417 1853 1995 715 984 17 153 Cuts at: 7714 7899 8344 325 Size: 185 445 1498 Fragments arranged by size: 1995 1853 1498 1255 984 715 445 417 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4839 4909 5330 Size: 274 80 33 51 1443 2862 70 421 Cuts at: 5330 5763 6801 7763 9475 96 Size: 433 1038 962 1712 138 Fragments arranged by size: 2862 1712 1443 1038 962 433 421 274 138 80 70 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5488 5520 9477 9509 5488 Size: 32 3957 32 5496 Fragments arranged by size: 5496 3957 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 9517 XbaI T'CTAG_A Cuts at: 6853 6853 Size: 9517 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2372 2558 3699 6612 2372 Size: 186 1141 2913 5277 Fragments arranged by size: 5277 2913 1141 186 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 9517 XmaI C'CCGG_G Cuts at: 1870 6842 1870 Size: 4972 4545 XmnI GAAnn'nnTTC Cuts at: 2405 3795 5165 7646 2405 Size: 1390 1370 2481 4276 Fragments arranged by size: 4276 2481 1390 1370 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 7325 276 Size: 53 83 186 936 5791 2468 Fragments arranged by size: 5791 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspOMI PspXI RsrII SacII SfiI SfoI SrfI SwaI