################################## # NO CUT ENZYMES ( 88 enzymes) # ################################## AatII AccII AccIII AclI AgeI AhdI AscI AseI AsnI AspEI AspI AviII AvrII BbeI BbrPI BciVI BcnI BglI BlnI BsaAI BseAI BsiEI BsiWI BsmBI BspDI BspEI BsrDI BssHII Bst1107I BstUI BstXI BstZ17I ClaI CpoI DraIII EagI Eam1105I EclXI Eco52I EcoRI EcoT22I FseI FspI KasI KspI MfeI MluI MroI MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI PacI PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SbfI SfiI SfoI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tth111I XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### Acc65I 46 GGTACC G^GTACC AccI 517 GTCTAC GT^[AC][GT]AC AcyI 2896 GACGCC G[GA]^CG[CT]C Aor51HI 434 AGCGCT AGC^GCT ApaI 2364 GGGCCC GGGCC^C Asp718I 46 GGTACC G^GTACC BalI 1760 TGGCCA TGG^CCA BclI 1313 TGATCA T^GATCA BlpI 73 GCTCAGC GC^T[ACGT]AGC Bpu1102I 73 GCTCAGC GC^T[ACGT]AGC BsaBI 241 GATCCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 2896 GACGCC G[GA]^CG[CT]C BsaI 3296 GGTCTC GGTCTC(1/5) Bsp1407I 1197 TGTACA T^GTACA BsrBI 1104 CCGCTC CCGCTC(-3/-3) BsrFI 2654 ACCGGC [GA]^CCGG[CT] BsrGI 1197 TGTACA T^GTACA BstAPI 1044 GCACCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 1858 TTCGAA TT^CGAA BstEII 337 GGTGACC G^GT[ACGT]ACC BstPI 337 GGTGACC G^GT[ACGT]ACC CelII 73 GCTCAGC GC^T[ACGT]AGC Cfr10I 2654 ACCGGC [GA]^CCGG[CT] DraI 2376 TTTAAA TTT^AAA DrdI 3704 GACTGACTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 434 AGCGCT AGC^GCT EcoO65I 337 GGTGACC G^GT[ACGT]ACC FbaI 1313 TGATCA T^GATCA HaeII 434 AGCGCT [GA]GCGC^[CT] HgaI 2896 GACGC GACGC(5/10) Hin1I 2896 GACGCC G[GA]^CG[CT]C HpaI 154 GTTAAC GTT^AAC KpnI 46 GGTACC GGTAC^C MaeII 1460 ACGT A^CGT MamI 241 GATCCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 1760 TGGCCA TGG^CCA MscI 1760 TGGCCA TGG^CCA NcoI 1976 CCATGG C^CATGG NspV 1858 TTCGAA TT^CGAA PflMI 414 CCAGATAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG SexAI 1955 ACCAGGT A^CC[AT]GGT SfuI 1858 TTCGAA TT^CGAA SspBI 1197 TGTACA T^GTACA Van91I 414 CCAGATAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AflII 51 CTTAAG C^TTAAG 127 CTTAAG AflIII 1172 ACATGT A^C[GA][CT]GT 2153 ACATGT AlwNI 1919 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG 2816 CAGCTACTG BbvCI 1110 CCTCAGC CCTCAGC(-5/-2) 2241 GCTGAGG BcgI 286 GCAGCATTTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 965 GCATCACTATCG BfrI 51 CTTAAG C^TTAAG 127 CTTAAG BglII 1871 AGATCT A^GATCT 4041 AGATCT BsaWI 681 TCCGGT [AT]^CCGG[AT] 1499 TCCGGT BsmI 2465 GAATGC GAATGC(1/-1) 3276 GAATGC BspLU11I 1172 ACATGT A^CATGT 2153 ACATGT BssSI 2224 CACGAG CACGAG(-5/-1) 544 CTCGTG EcoRV 1059 GATATC GAT^ATC 1495 GATATC FauI 504 CCCGC CCCGC(4/6) 947 CCCGC HindIII 3060 AAGCTT A^AGCTT 3446 AAGCTT PaeR7I 1214 CTCGAG C^TCGAG 3174 CTCGAG PvuII 2679 CAGCTG CAG^CTG 3524 CAGCTG ScaI 2060 AGTACT AGT^ACT 3319 AGTACT XcmI 2534 CCAAGGACAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2699 CCAGGATGAGTGTGG XhoI 1214 CTCGAG C^TCGAG 3174 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 107 enzymes) # ######################################### AciI AcsI AfaI AluI Alw44I AlwI AocI ApaLI ApoI Asp700I AspHI AvaI AvaII BamHI BanI BanII BbsI BbvI BfaI BmyI BpmI BpuAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspHI BspMI BsrI BssKI BstF5I BstNI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV NspI PleI PpuMI PstI RcaI RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmnI