(Circular) (Six-base) MAPSORT of: pFN21AE3469.seq Check: 1341 from: 1 to: 9058 pFN21AE3469 9058 bp With 182 enzymes: SgfI * January 30, 2014 17:23 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3060 3060 Size: 9058 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6868 278 Size: 53 83 186 936 5332 2468 Fragments arranged by size: 5332 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2523 6401 1937 Size: 586 3878 4594 Fragments arranged by size: 4594 3878 586 Acc65I G'GTAC_C Cuts at: 2051 6379 2051 Size: 4328 4730 AclI AA'CG_TT Cuts at: 1563 7185 7558 1563 Size: 5622 373 3063 Fragments arranged by size: 5622 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2513 2728 3536 3612 5349 5511 6063 6285 Size: 881 215 808 76 1737 162 552 222 Cuts at: 6285 7121 8505 8773 1632 Size: 836 1384 268 1917 Fragments arranged by size: 1917 1737 1384 881 836 808 552 268 222 215 162 76 AfeI AGC'GCT Cuts at: 2441 2441 Size: 9058 AflII C'TTAA_G Cuts at: 820 1017 2056 2132 820 Size: 197 1039 76 7746 Fragments arranged by size: 7746 1039 197 76 AflIII A'CryG_T Cuts at: 1203 3177 4158 7957 8934 1203 Size: 1974 981 3799 977 1327 Fragments arranged by size: 3799 1974 1327 981 977 AgeI A'CCGG_T Cuts at: 7889 7889 Size: 9058 AhdI GACnn_n'nnGTC Cuts at: 7787 7787 Size: 9058 AleI CACnn'nnGTG Cuts at: 1166 5150 1166 Size: 3984 5074 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2460 2858 2906 1184 Size: 1276 398 48 7336 Fragments arranged by size: 7336 1276 398 48 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4145 4177 4145 Size: 32 9026 AlwNI CAG_nnn'CTG Cuts at: 3929 4826 6801 8373 3929 Size: 897 1975 1572 4614 Fragments arranged by size: 4614 1975 1572 897 ApaI G_GGCC'C Cuts at: 4373 4373 Size: 9058 ApaLI G'TGCA_C Cuts at: 3047 4525 4635 7115 8271 3047 Size: 1478 110 2480 1156 3834 Fragments arranged by size: 3834 2480 1478 1156 110 ApoI r'AATT_y Cuts at: 1493 2127 2142 2213 5268 5612 6367 6593 6745 Size: 634 15 71 3055 344 755 226 152 Cuts at: 6745 8854 1493 Size: 2109 1697 Fragments arranged by size: 3055 2109 1697 755 634 344 226 152 71 15 AseI AT'TA_AT Cuts at: 160 1032 7612 160 Size: 872 6580 1606 Fragments arranged by size: 6580 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9058 AvaI C'yCGr_G Cuts at: 1870 1943 2010 3219 3394 5179 6383 1870 Size: 73 67 1209 175 1785 1204 4545 Fragments arranged by size: 4545 1785 1209 1204 175 73 67 BamHI G'GATC_C Cuts at: 2313 4563 5750 6388 6755 2313 Size: 2250 1187 638 367 4616 Fragments arranged by size: 4616 2250 1187 638 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2051 2895 5082 6379 7834 Size: 325 217 627 264 844 2187 1297 1455 Cuts at: 7834 618 Size: 1842 Fragments arranged by size: 2187 1842 1455 1297 844 627 325 264 217 BanII G_rGCy'C Cuts at: 1391 2730 3547 3572 4046 4373 4899 4931 4986 Size: 1339 817 25 474 327 526 32 55 Cuts at: 4986 5895 5915 6073 6155 6377 1391 Size: 909 20 158 82 222 4072 Fragments arranged by size: 4072 1339 909 817 526 474 327 222 158 82 55 32 25 20 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2015 2387 4594 5724 928 Size: 971 116 372 2207 1130 4262 Fragments arranged by size: 4262 2207 1130 971 372 116 BbvCI CC'TCA_GC Cuts at: 3116 4247 3116 Size: 1131 7927 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2280 2314 2959 2993 7249 7283 1903 Size: 34 343 34 645 34 4256 34 3678 Fragments arranged by size: 4256 3678 645 343 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6950 8160 6950 Size: 1210 7848 BclI T'GATC_A Cuts at: 1548 3318 1548 Size: 1770 7288 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 7669 136 Size: 107 122 71 631 605 5997 1525 Fragments arranged by size: 5997 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3876 6046 9053 3876 Size: 2170 3007 3881 Fragments arranged by size: 3881 3007 2170 BlpI GC'TnA_GC Cuts at: 2079 6475 2079 Size: 4396 4662 Bme1580I G_kGCm'C Cuts at: 1790 3051 3228 4373 4529 4639 7119 8275 1790 Size: 1261 177 1145 156 110 2480 1156 2573 Fragments arranged by size: 2573 2480 1261 1156 1145 177 156 110 BmgBI CAC'GTC Cuts at: 1318 3466 1318 Size: 2148 6910 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2661 3010 3017 Size: 416 53 83 399 24 1244 349 7 Cuts at: 3017 3259 4090 4702 6789 7747 442 Size: 242 831 612 2087 958 1753 Fragments arranged by size: 2087 1753 1244 958 831 612 416 399 349 242 83 53 24 7 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 9058 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3898 5608 3898 Size: 1710 7348 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2616 3044 3191 3561 3906 7718 1239 Size: 1377 428 147 370 345 3812 2579 Fragments arranged by size: 3812 2579 1377 428 370 345 147 Bpu10I CC'TnA_GC Cuts at: 2318 3116 4247 2318 Size: 798 1131 7129 Fragments arranged by size: 7129 1131 798 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4515 5217 7183 8048 8346 4515 Size: 702 1966 865 298 5227 Fragments arranged by size: 5227 1966 865 702 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5307 7721 882 Size: 617 3808 2414 2219 Fragments arranged by size: 3808 2414 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 6849 493 Size: 711 5645 2702 Fragments arranged by size: 5645 2702 711 BsaBI GATnn'nnATC Cuts at: 2250 9052 2250 Size: 6802 2256 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4902 6865 7247 7882 Size: 53 83 186 936 3369 1963 382 635 Cuts at: 7882 275 Size: 1451 Fragments arranged by size: 3369 1963 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2686 3504 7491 7889 8163 8310 8721 2686 Size: 818 3987 398 274 147 411 3023 Fragments arranged by size: 3987 3023 818 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3164 3194 4828 4858 1178 Size: 30 165 30 1761 30 1634 30 5378 Fragments arranged by size: 5378 1761 1634 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 4187 5967 6134 1186 Size: 3001 1780 167 4110 Fragments arranged by size: 4110 3001 1780 167 BseYI C'CCAG_C Cuts at: 3833 4682 5756 8261 8757 3833 Size: 849 1074 2505 496 4134 Fragments arranged by size: 4134 2505 1074 849 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2573 3014 3599 1163 Size: 573 837 441 585 6622 Fragments arranged by size: 6622 837 585 573 441 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6508 7269 8297 1540 Size: 275 186 4507 761 1028 2301 Fragments arranged by size: 4507 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2276 3051 4046 4529 4639 5895 6073 7119 7204 Size: 775 995 483 110 1256 178 1046 85 Cuts at: 7204 8275 2276 Size: 1071 3059 Fragments arranged by size: 3059 1256 1071 1046 995 775 483 178 110 85 BsmI GAATG_Cn' Cuts at: 4476 5287 6569 6662 4476 Size: 811 1282 93 6872 Fragments arranged by size: 6872 1282 811 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2276 2730 3051 3228 3547 3572 4046 Size: 399 486 454 321 177 319 25 474 Cuts at: 4046 4373 4529 4639 4899 4931 4986 5895 5915 Size: 327 156 110 260 32 55 909 20 Cuts at: 5915 6073 6155 6377 7119 7204 8275 1391 Size: 158 82 222 742 85 1071 2174 Fragments arranged by size: 2174 1071 909 742 486 474 454 399 327 321 319 260 222 177 158 156 110 85 82 55 32 25 20 BspEI T'CCGG_A Cuts at: 8721 8721 Size: 9058 BspHI T'CATG_A Cuts at: 2086 2763 3001 6947 8677 2086 Size: 677 238 3946 1730 2467 Fragments arranged by size: 3946 2467 1730 677 238 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2355 3060 3093 6413 844 Size: 1511 705 33 3320 3489 Fragments arranged by size: 3489 3320 1511 705 33 BsrBI CCG'CTC Cuts at: 1394 1823 3111 6945 1394 Size: 429 1288 3834 3507 Fragments arranged by size: 3834 3507 1288 429 BsrDI GCAATG_nn' Cuts at: 65 1248 7553 7727 65 Size: 1183 6305 174 1396 Fragments arranged by size: 6305 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4659 7702 7889 1700 Size: 110 2849 3043 187 2869 Fragments arranged by size: 3043 2869 2849 187 110 BsrGI T'GTAC_A Cuts at: 96 3202 96 Size: 3106 5952 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9058 BssSI C'ACGA_G Cuts at: 2549 4229 7118 8130 2549 Size: 1680 2889 1012 3477 Fragments arranged by size: 3477 2889 1680 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3055 794 Size: 2261 6797 BstBI TT'CG_AA Cuts at: 3864 7862 3864 Size: 3998 5060 BstEII G'GTnAC_C Cuts at: 1184 2342 1184 Size: 1158 7900 BstYI r'GATC_y Cuts at: 1972 2245 2313 2678 3736 3751 3876 4201 4563 Size: 273 68 365 1058 15 125 325 362 Cuts at: 4563 5750 6046 6388 6755 7140 7157 8609 8695 Size: 1187 296 342 367 385 17 1452 86 Cuts at: 8695 8707 9053 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1187 1058 385 367 365 362 346 342 325 296 273 125 86 68 17 15 12 Bsu36I CC'TnA_GG Cuts at: 2207 3741 6117 2207 Size: 1534 2376 5148 Fragments arranged by size: 5148 2376 1534 BtgI C'CryG_G Cuts at: 513 1065 3981 4365 5919 513 Size: 552 2916 384 1554 3652 Fragments arranged by size: 3652 2916 1554 552 384 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 8319 Fragments arranged by size: 8319 683 56 BtsI GCAGTG_nn' Cuts at: 2830 4036 4602 5958 6574 7368 7388 2830 Size: 1206 566 1356 616 794 20 4500 Fragments arranged by size: 4500 1356 1206 794 616 566 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 9058 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 9023 DraI TTT'AAA Cuts at: 4383 6362 4383 Size: 1979 7079 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5715 8065 809 Size: 507 4399 2350 1802 Fragments arranged by size: 4399 2350 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2850 3765 4362 Size: 54 1425 50 275 18 1020 915 597 Cuts at: 4362 6505 7394 9023 8 Size: 2143 889 1629 43 Fragments arranged by size: 2143 1629 1425 1020 915 889 597 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6505 1537 Size: 275 4693 4090 Fragments arranged by size: 4693 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2688 3813 3909 3984 4051 4296 4646 5187 Size: 1335 1125 96 75 67 245 350 541 Cuts at: 5187 5337 6189 6988 1353 Size: 150 852 799 3423 Fragments arranged by size: 3423 1335 1125 852 799 541 350 245 150 96 75 67 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 7645 8019 8165 387 Size: 1064 138 6056 374 146 1280 Fragments arranged by size: 6056 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4044 5893 6071 4044 Size: 1849 178 7031 Fragments arranged by size: 7031 1849 178 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2513 2616 2728 3044 3191 3536 3561 Size: 393 881 103 112 316 147 345 25 Cuts at: 3561 3612 3906 5349 5511 6063 6285 7121 7718 Size: 51 294 1443 162 552 222 836 597 Cuts at: 7718 8505 8773 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1443 881 836 787 597 552 393 345 316 294 268 222 162 147 112 103 51 25 EcoNI CCTnn'n_nnAGG Cuts at: 2552 5765 6146 2552 Size: 3213 381 5464 Fragments arranged by size: 5464 3213 381 EcoO109I rG'GnC_Cy Cuts at: 2545 2938 3211 3745 4370 4667 6091 2545 Size: 393 273 534 625 297 1424 5512 Fragments arranged by size: 5512 1424 625 534 393 297 273 EcoRI G'AATT_C Cuts at: 6367 6745 6367 Size: 378 8680 EcoRV GAT'ATC Cuts at: 3066 3502 3066 Size: 436 8622 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2099 3541 4795 5570 5748 2099 Size: 1442 1254 775 178 5409 Fragments arranged by size: 5409 1442 1254 775 178 FspI TGC'GCA Cuts at: 7564 7564 Size: 9058 HaeII r_GCGC'y Cuts at: 1678 2443 8205 1678 Size: 765 5762 2531 Fragments arranged by size: 5762 2531 765 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2225 2257 2656 2688 3576 Size: 32 135 32 653 32 399 32 888 Cuts at: 3576 3608 3721 3753 3766 3798 4028 4060 4295 Size: 32 113 32 13 32 230 32 235 Cuts at: 4295 4327 7699 7731 7773 7805 1373 Size: 32 3372 32 42 32 2626 Fragments arranged by size: 3372 2626 888 653 399 235 230 135 113 42 32 32 32 32 32 32 32 32 32 32 32 13 HincII GTy'rAC Cuts at: 669 1657 1938 2161 3689 4128 6402 6648 669 Size: 988 281 223 1528 439 2274 246 3079 Fragments arranged by size: 3079 2274 1528 988 439 281 246 223 HindIII A'AGCT_T Cuts at: 748 5065 5451 748 Size: 4317 386 4355 Fragments arranged by size: 4355 4317 386 HpaI GTT'AAC Cuts at: 2161 6648 2161 Size: 4487 4571 KpnI G_GTAC'C Cuts at: 2055 6383 2055 Size: 4328 4730 MfeI C'AATT_G Cuts at: 6657 6657 Size: 9058 MluI A'CGCG_T Cuts at: 8934 8934 Size: 9058 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3162 8172 8356 1305 Size: 1857 5010 184 2007 Fragments arranged by size: 5010 2007 1857 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3767 10 Size: 54 1425 343 1935 5301 Fragments arranged by size: 5301 1935 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2091 2762 3038 3600 4233 5150 5847 Size: 648 925 671 276 562 633 917 697 Cuts at: 5847 7016 7375 7534 518 Size: 1169 359 159 2042 Fragments arranged by size: 2042 1169 925 917 697 671 648 633 562 359 276 159 MspA1I CmG'CkG Cuts at: 1606 2259 2515 2748 4686 5531 6474 7151 8299 Size: 653 256 233 1938 845 943 677 1148 Cuts at: 8299 8544 1606 Size: 245 2120 Fragments arranged by size: 2120 1938 1148 943 845 677 653 256 245 233 NcoI C'CATG_G Cuts at: 513 1065 3981 513 Size: 552 2916 5590 Fragments arranged by size: 5590 2916 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 9058 NheI G'CTAG_C Cuts at: 1052 1052 Size: 9058 NotI GC'GGCC_GC Cuts at: 6505 6505 Size: 9058 NspI r_CATG'y Cuts at: 1132 1229 2458 3181 4162 4312 5775 6416 7961 Size: 97 1229 723 981 150 1463 641 1545 Cuts at: 7961 1132 Size: 2229 Fragments arranged by size: 2229 1545 1463 1229 981 723 641 150 97 PasI CC'CwG_GG Cuts at: 1348 5513 1348 Size: 4165 4893 PciI A'CATG_T Cuts at: 3177 4158 7957 3177 Size: 981 3799 4278 Fragments arranged by size: 4278 3799 981 PflMI CCAn_nnn'nTGG Cuts at: 1108 2425 1108 Size: 1317 7741 PfoI T'CCnGG_A Cuts at: 3922 6320 3922 Size: 2398 6660 PmeI GTTT'AAAC Cuts at: 6362 6362 Size: 9058 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5237 5269 7127 7159 8640 8672 5237 Size: 32 1858 32 1481 32 5623 Fragments arranged by size: 5623 1858 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2938 3211 4667 6091 2938 Size: 273 1456 1424 5905 Fragments arranged by size: 5905 1456 1424 273 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 9058 PsiI TTA'TAA Cuts at: 5642 6628 5642 Size: 986 8072 PspOMI G'GGCC_C Cuts at: 4369 4369 Size: 9058 PspXI vC'TCGA_Gb Cuts at: 3219 5179 3219 Size: 1960 7098 PstI C_TGCA'G Cuts at: 830 2837 3860 4914 6111 6410 830 Size: 2007 1023 1054 1197 299 3478 Fragments arranged by size: 3478 2007 1197 1054 1023 299 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9058 PvuII CAG'CTG Cuts at: 4686 5531 4686 Size: 845 8213 SacI G_AGCT'C Cuts at: 4046 5895 6073 4046 Size: 1849 178 7031 Fragments arranged by size: 7031 1849 178 SalI G'TCGA_C Cuts at: 1936 6400 1936 Size: 4464 4594 SapI GCTCTTCn'nnn_ Cuts at: 3909 3984 4051 4296 3909 Size: 75 67 245 8671 Fragments arranged by size: 8671 245 75 67 SbfI CC_TGCA'GG Cuts at: 6410 6410 Size: 9058 ScaI AGT'ACT Cuts at: 4067 5326 7306 9018 4067 Size: 1259 1980 1712 4107 Fragments arranged by size: 4107 1980 1712 1259 SexAI A'CCwGG_T Cuts at: 3960 3960 Size: 9058 SfcI C'TryA_G Cuts at: 826 1045 2524 2620 2833 2909 3304 3856 4403 Size: 219 1479 96 213 76 395 552 547 Cuts at: 4403 4910 5157 6107 6406 7541 8222 8413 826 Size: 507 247 950 299 1135 681 191 1471 Fragments arranged by size: 1479 1471 1135 950 681 552 547 507 395 299 247 219 213 191 96 76 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9058 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9058 SmaI CCC'GGG Cuts at: 1872 6385 1872 Size: 4513 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2056 2132 3219 4530 5179 5232 Size: 197 926 113 76 1087 1311 649 53 Cuts at: 5232 7162 8063 8325 820 Size: 1930 901 262 1553 Fragments arranged by size: 1930 1553 1311 1087 926 901 649 262 197 113 76 53 SnaBI TAC'GTA Cuts at: 493 6849 493 Size: 6356 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 9058 SphI G_CATG'C Cuts at: 1132 6416 1132 Size: 5284 3774 SspI AAT'ATT Cuts at: 5 52 6982 5 Size: 47 6930 2081 Fragments arranged by size: 6930 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 9058 StyI C'CwwG_G Cuts at: 513 1065 2166 3701 3981 4539 4847 6147 6497 Size: 552 1101 1535 280 558 308 1300 350 Cuts at: 6497 513 Size: 3074 Fragments arranged by size: 3074 1535 1300 1101 558 552 350 308 280 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2432 2837 2841 3495 4696 5100 5482 Size: 1255 852 405 4 654 1201 404 382 Cuts at: 5482 5813 7085 7102 7255 7440 7885 325 Size: 331 1272 17 153 185 445 1498 Fragments arranged by size: 1498 1272 1255 1201 852 654 445 405 404 382 331 185 153 17 4 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3174 3202 4065 Size: 274 80 33 51 1443 1197 28 863 Cuts at: 4065 5324 7304 9016 96 Size: 1259 1980 1712 138 Fragments arranged by size: 1980 1712 1443 1259 1197 863 274 138 80 51 33 28 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4828 4860 9018 9050 4828 Size: 32 4158 32 4836 Fragments arranged by size: 4836 4158 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 9058 XbaI T'CTAG_A Cuts at: 6394 6394 Size: 9058 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4546 4711 4546 Size: 165 8893 XhoI C'TCGA_G Cuts at: 1943 3219 5179 1943 Size: 1276 1960 5822 Fragments arranged by size: 5822 1960 1276 XmaI C'CCGG_G Cuts at: 1870 6383 1870 Size: 4513 4545 XmnI GAAnn'nnTTC Cuts at: 2709 4518 5616 7187 2709 Size: 1809 1098 1571 4580 Fragments arranged by size: 4580 1809 1571 1098 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6866 276 Size: 53 83 186 936 5332 2468 Fragments arranged by size: 5332 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstXI BstZ17I DraIII FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI