(Linear) (Six-base) MAPSORT of: KIEE3469.seq Check: 1495 from: 1 to: 4353 KIEE3469.orf With 182 enzymes: SgfI * January 29, 2014 11:45 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1055 4353 Size: 1055 3298 AccI GT'mk_AC Cuts at: 0 518 4353 Size: 518 3835 Acc65I G'GTAC_C Cuts at: 0 46 4353 Size: 46 4307 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 508 723 1531 1607 3344 3506 4058 4280 Size: 508 215 808 76 1737 162 552 222 Cuts at: 4280 4353 Size: 73 Fragments arranged by size: 1737 808 552 508 222 215 162 76 73 AfeI AGC'GCT Cuts at: 0 436 4353 Size: 436 3917 AflII C'TTAA_G Cuts at: 0 51 127 4353 Size: 51 76 4226 Fragments arranged by size: 4226 76 51 AflIII A'CryG_T Cuts at: 0 1172 2153 4353 Size: 1172 981 2200 Fragments arranged by size: 2200 1172 981 AleI CACnn'nnGTG Cuts at: 0 3145 4353 Size: 3145 1208 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 455 853 901 4353 Size: 455 398 48 3452 Fragments arranged by size: 3452 455 398 48 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2140 2172 4353 Size: 2140 32 2181 Fragments arranged by size: 2181 2140 32 AlwNI CAG_nnn'CTG Cuts at: 0 1924 2821 4353 Size: 1924 897 1532 Fragments arranged by size: 1924 1532 897 ApaI G_GGCC'C Cuts at: 0 2368 4353 Size: 2368 1985 ApaLI G'TGCA_C Cuts at: 0 1042 2520 2630 4353 Size: 1042 1478 110 1723 Fragments arranged by size: 1723 1478 1042 110 ApoI r'AATT_y Cuts at: 0 122 137 208 3263 3607 4353 Size: 122 15 71 3055 344 746 Fragments arranged by size: 3055 746 344 122 71 15 AvaI C'yCGr_G Cuts at: 0 5 1214 1389 3174 4353 Size: 5 1209 175 1785 1179 Fragments arranged by size: 1785 1209 1179 175 5 BamHI G'GATC_C Cuts at: 0 308 2558 3745 4353 Size: 308 2250 1187 608 Fragments arranged by size: 2250 1187 608 308 BanI G'GyrC_C Cuts at: 0 46 890 3077 4353 Size: 46 844 2187 1276 Fragments arranged by size: 2187 1276 844 46 BanII G_rGCy'C Cuts at: 0 725 1542 1567 2041 2368 2894 2926 2981 Size: 725 817 25 474 327 526 32 55 Cuts at: 2981 3890 3910 4068 4150 4353 Size: 909 20 158 82 203 Fragments arranged by size: 909 817 725 526 474 327 203 158 82 55 32 25 20 BbsI GAAGACnn'nnnn_ Cuts at: 0 10 382 2589 3719 4353 Size: 10 372 2207 1130 634 Fragments arranged by size: 2207 1130 634 372 10 BbvCI CC'TCA_GC Cuts at: 0 1111 2242 4353 Size: 1111 1131 2111 Fragments arranged by size: 2111 1131 1111 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 275 309 954 988 4353 Size: 275 34 645 34 3365 Fragments arranged by size: 3365 645 275 34 34 BclI T'GATC_A Cuts at: 0 1313 4353 Size: 1313 3040 BglII A'GATC_T Cuts at: 0 1871 4041 4353 Size: 1871 2170 312 Fragments arranged by size: 2170 1871 312 BlpI GC'TnA_GC Cuts at: 0 74 4353 Size: 74 4279 Bme1580I G_kGCm'C Cuts at: 0 1046 1223 2368 2524 2634 4353 Size: 1046 177 1145 156 110 1719 Fragments arranged by size: 1719 1145 1046 177 156 110 BmgBI CAC'GTC Cuts at: 0 1461 4353 Size: 1461 2892 BmrI ACTGGGnnnn_n' Cuts at: 0 656 1005 1012 1254 2085 2697 4353 Size: 656 349 7 242 831 612 1656 Fragments arranged by size: 1656 831 656 612 349 242 7 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1893 3603 4353 Size: 1893 1710 750 Fragments arranged by size: 1893 1710 750 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 611 1039 1186 1556 1901 4353 Size: 611 428 147 370 345 2452 Fragments arranged by size: 2452 611 428 370 345 147 Bpu10I CC'TnA_GC Cuts at: 0 313 1111 2242 4353 Size: 313 798 1131 2111 Fragments arranged by size: 2111 1131 798 313 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2510 3212 4353 Size: 2510 702 1141 Fragments arranged by size: 2510 1141 702 BsaI GGTCTCn'nnnn_ Cuts at: 0 3302 4353 Size: 3302 1051 BsaBI GATnn'nnATC Cuts at: 0 245 4353 Size: 245 4108 BsaHI Gr'CG_yC Cuts at: 0 2897 4353 Size: 2897 1456 BsaWI w'CCGG_w Cuts at: 0 681 1499 4353 Size: 681 818 2854 Fragments arranged by size: 2854 818 681 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1159 1189 2823 2853 4353 Size: 1159 30 1634 30 1500 Fragments arranged by size: 1634 1500 1159 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 2182 3962 4129 4353 Size: 2182 1780 167 224 Fragments arranged by size: 2182 1780 224 167 BseYI C'CCAG_C Cuts at: 0 1828 2677 3751 4353 Size: 1828 849 1074 602 Fragments arranged by size: 1828 1074 849 602 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 568 1009 1594 4353 Size: 568 441 585 2759 Fragments arranged by size: 2759 585 568 441 BsiHKAI G_wGCw'C Cuts at: 0 271 1046 2041 2524 2634 3890 4068 4353 Size: 271 775 995 483 110 1256 178 285 Fragments arranged by size: 1256 995 775 483 285 271 178 110 BsmI GAATG_Cn' Cuts at: 0 2471 3282 4353 Size: 2471 811 1071 Fragments arranged by size: 2471 1071 811 Bsp1286I G_dGCh'C Cuts at: 0 271 725 1046 1223 1542 1567 2041 2368 Size: 271 454 321 177 319 25 474 327 Cuts at: 2368 2524 2634 2894 2926 2981 3890 3910 4068 Size: 156 110 260 32 55 909 20 158 Cuts at: 4068 4150 4353 Size: 82 203 Fragments arranged by size: 909 474 454 327 321 319 271 260 203 177 158 156 110 82 55 32 25 20 BspHI T'CATG_A Cuts at: 0 81 758 996 4353 Size: 81 677 238 3357 Fragments arranged by size: 3357 677 238 81 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 350 1055 1088 4353 Size: 350 705 33 3265 Fragments arranged by size: 3265 705 350 33 BsrBI CCG'CTC Cuts at: 0 1106 4353 Size: 1106 3247 BsrFI r'CCGG_y Cuts at: 0 2654 4353 Size: 2654 1699 BsrGI T'GTAC_A Cuts at: 0 1197 4353 Size: 1197 3156 BssSI C'ACGA_G Cuts at: 0 544 2224 4353 Size: 544 1680 2129 Fragments arranged by size: 2129 1680 544 BstAPI GCAn_nnn'nTGC Cuts at: 0 1050 4353 Size: 1050 3303 BstBI TT'CG_AA Cuts at: 0 1859 4353 Size: 1859 2494 BstEII G'GTnAC_C Cuts at: 0 337 4353 Size: 337 4016 BstYI r'GATC_y Cuts at: 0 240 308 673 1731 1746 1871 2196 2558 Size: 240 68 365 1058 15 125 325 362 Cuts at: 2558 3745 4041 4353 Size: 1187 296 312 Fragments arranged by size: 1187 1058 365 362 325 312 296 240 125 68 15 Bsu36I CC'TnA_GG Cuts at: 0 202 1736 4112 4353 Size: 202 1534 2376 241 Fragments arranged by size: 2376 1534 241 202 BtgI C'CryG_G Cuts at: 0 1976 2360 3914 4353 Size: 1976 384 1554 439 Fragments arranged by size: 1976 1554 439 384 BtsI GCAGTG_nn' Cuts at: 0 825 2031 2597 3953 4353 Size: 825 1206 566 1356 400 Fragments arranged by size: 1356 1206 825 566 400 DraI TTT'AAA Cuts at: 0 2378 4353 Size: 2378 1975 DrdI GACnn_nn'nnGTC Cuts at: 0 3710 4353 Size: 3710 643 EaeI y'GGCC_r Cuts at: 0 845 1760 2357 4353 Size: 845 915 597 1996 Fragments arranged by size: 1996 915 845 597 EarI CTCTTCn'nnn_ Cuts at: 0 683 1808 1904 1979 2046 2291 2641 3182 Size: 683 1125 96 75 67 245 350 541 Cuts at: 3182 3332 4184 4353 Size: 150 852 169 Fragments arranged by size: 1125 852 683 541 350 245 169 150 96 75 67 EcoICRI GAG'CTC Cuts at: 0 2039 3888 4066 4353 Size: 2039 1849 178 287 Fragments arranged by size: 2039 1849 287 178 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 508 611 723 1039 1186 1531 1556 1607 Size: 508 103 112 316 147 345 25 51 Cuts at: 1607 1901 3344 3506 4058 4280 4353 Size: 294 1443 162 552 222 73 Fragments arranged by size: 1443 552 508 345 316 294 222 162 147 112 103 73 51 25 EcoNI CCTnn'n_nnAGG Cuts at: 0 547 3760 4141 4353 Size: 547 3213 381 212 Fragments arranged by size: 3213 547 381 212 EcoO109I rG'GnC_Cy Cuts at: 0 540 933 1206 1740 2365 2662 4086 4353 Size: 540 393 273 534 625 297 1424 267 Fragments arranged by size: 1424 625 540 534 393 297 273 267 EcoRV GAT'ATC Cuts at: 0 1061 1497 4353 Size: 1061 436 2856 Fragments arranged by size: 2856 1061 436 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 94 1536 2790 3565 3743 4353 Size: 94 1442 1254 775 178 610 Fragments arranged by size: 1442 1254 775 610 178 94 HaeII r_GCGC'y Cuts at: 0 438 4353 Size: 438 3915 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 220 252 651 683 1571 1603 1716 1748 Size: 220 32 399 32 888 32 113 32 Cuts at: 1748 1761 1793 2023 2055 2290 2322 4353 Size: 13 32 230 32 235 32 2031 Fragments arranged by size: 2031 888 399 235 230 220 113 32 32 32 32 32 32 32 13 HincII GTy'rAC Cuts at: 0 156 1684 2123 4353 Size: 156 1528 439 2230 Fragments arranged by size: 2230 1528 439 156 HindIII A'AGCT_T Cuts at: 0 3060 3446 4353 Size: 3060 386 907 Fragments arranged by size: 3060 907 386 HpaI GTT'AAC Cuts at: 0 156 4353 Size: 156 4197 KpnI G_GTAC'C Cuts at: 0 50 4353 Size: 50 4303 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1157 4353 Size: 1157 3196 MscI TGG'CCA Cuts at: 0 1762 4353 Size: 1762 2591 MslI CAynn'nnrTG Cuts at: 0 86 757 1033 1595 2228 3145 3842 4353 Size: 86 671 276 562 633 917 697 511 Fragments arranged by size: 917 697 671 633 562 511 276 86 MspA1I CmG'CkG Cuts at: 0 254 510 743 2681 3526 4353 Size: 254 256 233 1938 845 827 Fragments arranged by size: 1938 845 827 256 254 233 NcoI C'CATG_G Cuts at: 0 1976 4353 Size: 1976 2377 NspI r_CATG'y Cuts at: 0 453 1176 2157 2307 3770 4353 Size: 453 723 981 150 1463 583 Fragments arranged by size: 1463 981 723 583 453 150 PasI CC'CwG_GG Cuts at: 0 3508 4353 Size: 3508 845 PciI A'CATG_T Cuts at: 0 1172 2153 4353 Size: 1172 981 2200 Fragments arranged by size: 2200 1172 981 PflMI CCAn_nnn'nTGG Cuts at: 0 420 4353 Size: 420 3933 PfoI T'CCnGG_A Cuts at: 0 1917 4315 4353 Size: 1917 2398 38 Fragments arranged by size: 2398 1917 38 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 3232 3264 4353 Size: 3232 32 1089 Fragments arranged by size: 3232 1089 32 PpuMI rG'GwC_Cy Cuts at: 0 933 1206 2662 4086 4353 Size: 933 273 1456 1424 267 Fragments arranged by size: 1456 1424 933 273 267 PsiI TTA'TAA Cuts at: 0 3637 4353 Size: 3637 716 PspOMI G'GGCC_C Cuts at: 0 2364 4353 Size: 2364 1989 PspXI vC'TCGA_Gb Cuts at: 0 1214 3174 4353 Size: 1214 1960 1179 Fragments arranged by size: 1960 1214 1179 PstI C_TGCA'G Cuts at: 0 832 1855 2909 4106 4353 Size: 832 1023 1054 1197 247 Fragments arranged by size: 1197 1054 1023 832 247 PvuII CAG'CTG Cuts at: 0 2681 3526 4353 Size: 2681 845 827 Fragments arranged by size: 2681 845 827 SacI G_AGCT'C Cuts at: 0 2041 3890 4068 4353 Size: 2041 1849 178 285 Fragments arranged by size: 2041 1849 285 178 SapI GCTCTTCn'nnn_ Cuts at: 0 1904 1979 2046 2291 4353 Size: 1904 75 67 245 2062 Fragments arranged by size: 2062 1904 245 75 67 ScaI AGT'ACT Cuts at: 0 2062 3321 4353 Size: 2062 1259 1032 Fragments arranged by size: 2062 1259 1032 SexAI A'CCwGG_T Cuts at: 0 1955 4353 Size: 1955 2398 SfcI C'TryA_G Cuts at: 0 519 615 828 904 1299 1851 2398 2905 Size: 519 96 213 76 395 552 547 507 Cuts at: 2905 3152 4102 4353 Size: 247 950 251 Fragments arranged by size: 950 552 547 519 507 395 251 247 213 96 76 SmlI C'TyrA_G Cuts at: 0 51 127 1214 2525 3174 3227 4353 Size: 51 76 1087 1311 649 53 1126 Fragments arranged by size: 1311 1126 1087 649 76 53 51 StyI C'CwwG_G Cuts at: 0 161 1696 1976 2534 2842 4142 4353 Size: 161 1535 280 558 308 1300 211 Fragments arranged by size: 1535 1300 558 308 280 211 161 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 427 832 836 1490 2691 3095 3477 3808 Size: 427 405 4 654 1201 404 382 331 Cuts at: 3808 4353 Size: 545 Fragments arranged by size: 1201 654 545 427 405 404 382 331 4 TatI w'GTAC_w Cuts at: 0 1169 1197 2060 3319 4353 Size: 1169 28 863 1259 1034 Fragments arranged by size: 1259 1169 1034 863 28 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2823 2855 4353 Size: 2823 32 1498 Fragments arranged by size: 2823 1498 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 2541 2706 4353 Size: 2541 165 1647 Fragments arranged by size: 2541 1647 165 XhoI C'TCGA_G Cuts at: 0 1214 3174 4353 Size: 1214 1960 1179 Fragments arranged by size: 1960 1214 1179 XmnI GAAnn'nnTTC Cuts at: 0 704 2513 3611 4353 Size: 704 1809 1098 742 Fragments arranged by size: 1809 1098 742 704 Enzymes that do cut: AarI AccI Acc65I AcuI AfeI AflII AflIII AleI AlfI AloI AlwNI ApaI ApaLI ApoI AvaI BamHI BanI BanII BbsI BbvCI BcgI BclI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtsI DraI DrdI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FalI HaeII Hin4I HincII HindIII HpaI KpnI MmeI MscI MslI MspA1I NcoI NspI PasI PciI PflMI PfoI PpiI PpuMI PsiI PspOMI PspXI PstI PvuII SacI SapI ScaI SexAI SfcI SmlI StyI TaqII TatI TstI XcmI XhoI XmnI Enzymes that do not cut: AatII AclI AgeI AhdI AscI AseI AsiSI AvrII BaeI BbeI BciVI BfrBI BglI BmtI BsaAI BsiEI BsiWI BsmBI BspEI BsrDI BssHII BstXI BstZ17I BtgZI ClaI CspCI DraIII EagI EciI EcoRI FseI FspI FspAI KasI MfeI MluI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PmeI PmlI PshAI PsrI PvuI RsrII SacII SalI SanDI SbfI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I XbaI XmaI ZraI