################################## # NO CUT ENZYMES ( 94 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AhdI AlwI AocI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BamHI BciVI BclI BfrI BglII BlnI BsaI BseAI BsiWI BsmBI BspEI BspHI BspMI BssHII Bst1107I BstAPI BstBI BstXI BstYI BstZ17I Bsu36I CpoI DraI DrdI Eam1105I Eco81I EcoRI EcoRV FbaI FspI HgaI HincII HindII HindIII HpaI KpnI MflI MluI MluNI MroI MscI NcoI NdeI NotI NruI NspV PacI PaeR7I PflFI PleI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SalI SbfI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tth111I XbaI XcmI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 63 enzymes) # ################################### AcsI 1051 GAATTT [GA]^AATT[CT] AcyI 56 GGCGCC G[GA]^CG[CT]C AflIII 828 ACATGT A^C[GA][CT]GT AgeI 575 ACCGGT A^CCGGT Alw44I 373 GTGCAC G^TGCAC Aor51HI 1757 AGCGCT AGC^GCT ApaI 529 GGGCCC GGGCC^C ApaLI 373 GTGCAC G^TGCAC ApoI 1051 GAATTT [GA]^AATT[CT] BbeI 56 GGCGCC GGCGC^C BbrPI 1574 CACGTG CAC^GTG BbsI 1648 GAAGAC GAAGAC(2/6) BbvCI 890 CCTCAGC CCTCAGC(-5/-2) BcgI 895 GCACAGTACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BpuAI 1648 GAAGAC GAAGAC(2/6) BsaAI 1574 CACGTG [CT]AC^GT[GA] BsaBI 1720 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 56 GGCGCC G[GA]^CG[CT]C BsaWI 575 ACCGGT [AT]^CCGG[AT] BseRI 861 CTCCTC GAGGAG(10/8) BsgI 1510 GTGCAG GTGCAG(16/14) Bsp1407I 825 TGTACA T^GTACA BspDI 121 ATCGAT AT^CGAT BspLU11I 828 ACATGT A^CATGT BsrDI 311 GCAATG GCAATG(2/0) BsrGI 825 TGTACA T^GTACA BssSI 370 CTCGTG CACGAG(-5/-1) BstEII 792 GGTTACC G^GT[ACGT]ACC BstPI 792 GGTTACC G^GT[ACGT]ACC ClaI 121 ATCGAT AT^CGAT DraIII 1537 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG Eco47III 1757 AGCGCT AGC^GCT EcoNI 649 CCTCTGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 792 GGTTACC G^GT[ACGT]ACC EcoT14I 702 CCTTGG C^C[AT][AT]GG EcoT22I 709 ATGCAT ATGCA^T FseI 417 GGCCGGCC GGCCGG^CC Hin1I 56 GGCGCC G[GA]^CG[CT]C KasI 56 GGCGCC G^GCGCC MamI 1720 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 1065 CAATTG C^AATTG MseI 1780 TTAA T^TAA MslI 952 CACATGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MunI 1065 CAATTG C^AATTG NaeI 418 GCCGGC GCC^GGC NarI 56 GGCGCC GG^CGCC NgoMIV 418 GCCGGC G^CCGGC NsiI 709 ATGCAT ATGCA^T NspI 828 ACATGT [GA]CATG^[CT] PinAI 575 ACCGGT A^CCGGT PmaCI 1574 CACGTG CAC^GTG PmlI 1574 CACGTG CAC^GTG PpuMI 840 AGGTCCT [GA]G^G[AT]CC[CT] SacI 208 GAGCTC GAGCT^C SexAI 1538 ACCAGGT A^CC[AT]GGT SfoI 56 GGCGCC GGC^GCC SmaI 160 CCCGGG CCC^GGG SmlI 379 CTCAAG C^T[CT][GA]AG SspBI 825 TGTACA T^GTACA StyI 702 CCTTGG C^C[AT][AT]GG Tru9I 1780 TTAA T^TAA XmaCI 160 CCCGGG C^CCGGG XmaI 160 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AlwNI 457 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG 554 CAGTGGCTG BanI 56 GGCGCC G^G[CT][GA]CC 429 GGTGCC BlpI 150 GCTCAGC GC^T[ACGT]AGC 1399 GCTCAGC Bpu1102I 150 GCTCAGC GC^T[ACGT]AGC 1399 GCTCAGC BsiEI 99 CGGCCG CG[GA][CT]^CG 416 CGGCCG BsmAI 727 GAGAC GTCTC(1/5) 600 GTCTC BsmI 707 GAATGC GAATGC(1/-1) 1146 GCATTC CelII 150 GCTCAGC GC^T[ACGT]AGC 1399 GCTCAGC DsaI 102 CCGCGG C^C[GA][CT]GG 524 CCGCGG EaeI 99 CGGCCG [CT]^GGCC[GA] 416 CGGCCG EagI 99 CGGCCG C^GGCCG 416 CGGCCG EclXI 99 CGGCCG C^GGCCG 416 CGGCCG Eco52I 99 CGGCCG C^GGCCG 416 CGGCCG KspI 102 CCGCGG CCGC^GG 524 CCGCGG MaeII 1575 ACGT A^CGT 1739 ACGT NheI 1276 GCTAGC G^CTAGC 1633 GCTAGC PflMI 47 CCAAGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 86 CCATCCGCTGG SacII 102 CCGCGG CCGC^GG 524 CCGCGG SapI 132 GCTCTTC GCTCTTC(1/4) 210 GCTCTTC Tsp45I 976 GTCAC ^GT[GC]AC 1368 GTCAC Van91I 47 CCAAGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 86 CCATCCGCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 80 enzymes) # ######################################### AccII AciI AfaI AluI AspHI AvaI AvaII BanII BbvI BcnI BfaI BglI BmyI BpmI BsaJI BsiHKAI BsiYI BslI BsmFI BsoBI Bsp1286I BsrBI BsrFI BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII EarI EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MnlI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PstI PvuII RsaI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI TaqI TfiI TseI Tsp509I TspRI TthHB8I