(Circular) (Six-base) MAPSORT of: pFN21AB7816.seq Check: 6033 from: 1 to: 6493 pFN21AB7816 6493 bp With 182 enzymes: SgfI * March 28, 2012 12:35 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4303 278 Size: 53 83 186 936 2767 2468 Fragments arranged by size: 2767 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3836 1937 Size: 1899 4594 Acc65I G'GTAC_C Cuts at: 3814 3814 Size: 6493 AclI AA'CG_TT Cuts at: 1563 4620 4993 1563 Size: 3057 373 3063 Fragments arranged by size: 3063 3057 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3486 3488 4556 5940 6208 1632 Size: 1854 2 1068 1384 268 1917 Fragments arranged by size: 1917 1854 1384 1068 268 2 AfeI AGC'GCT Cuts at: 3764 3764 Size: 6493 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6296 AflIII A'CryG_T Cuts at: 1203 2833 5392 6369 1203 Size: 1630 2559 977 1327 Fragments arranged by size: 2559 1630 1327 977 AgeI A'CCGG_T Cuts at: 2580 5324 2580 Size: 2744 3749 AhdI GACnn_n'nnGTC Cuts at: 5222 5222 Size: 6493 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6493 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2297 3373 1184 Size: 1113 1076 4304 Fragments arranged by size: 4304 1113 1076 AlwNI CAG_nnn'CTG Cuts at: 2467 2564 4236 5808 2467 Size: 97 1672 1572 3152 Fragments arranged by size: 3152 1672 1572 97 ApaI G_GGCC'C Cuts at: 2538 2538 Size: 6493 ApaLI G'TGCA_C Cuts at: 2378 4550 5706 2378 Size: 2172 1156 3165 Fragments arranged by size: 3165 2172 1156 ApoI r'AATT_y Cuts at: 1493 3056 3802 4028 4180 6289 1493 Size: 1563 746 226 152 2109 1697 Fragments arranged by size: 2109 1697 1563 746 226 152 AseI AT'TA_AT Cuts at: 160 1032 5047 160 Size: 872 4015 1606 Fragments arranged by size: 4015 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6493 AvaI C'yCGr_G Cuts at: 1870 1943 2165 2210 2543 3818 1870 Size: 73 222 45 333 1275 4545 Fragments arranged by size: 4545 1275 333 222 73 45 BamHI G'GATC_C Cuts at: 3823 4190 3823 Size: 367 6126 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2061 2434 3814 5269 618 Size: 325 217 627 274 373 1380 1455 1842 Fragments arranged by size: 1842 1455 1380 627 373 325 274 217 BanII G_rGCy'C Cuts at: 1391 2211 2217 2538 3812 1391 Size: 820 6 321 1274 4072 Fragments arranged by size: 4072 1274 820 321 6 BbeI G_GCGC'C Cuts at: 2065 2065 Size: 6493 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3660 928 Size: 971 1761 3761 Fragments arranged by size: 3761 1761 971 BbvCI CC'TCA_GC Cuts at: 2896 2896 Size: 6493 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2889 2923 4684 4718 1903 Size: 34 952 34 1761 34 3678 Fragments arranged by size: 3678 1761 952 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4385 5595 4385 Size: 1210 5283 BclI T'GATC_A Cuts at: 1548 1548 Size: 6493 BfrBI ATG'CAT Cuts at: 2716 2716 Size: 6493 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2408 2417 Size: 107 122 71 631 605 337 399 9 Cuts at: 2417 2533 5104 136 Size: 116 2571 1525 Fragments arranged by size: 2571 1525 631 605 399 337 122 116 107 71 9 BglII A'GATC_T Cuts at: 6488 6488 Size: 6493 BlpI GC'TnA_GC Cuts at: 2156 3405 3910 2156 Size: 1249 505 4739 Fragments arranged by size: 4739 1249 505 Bme1580I G_kGCm'C Cuts at: 1790 2382 2439 2538 3206 4554 5710 1790 Size: 592 57 99 668 1348 1156 2573 Fragments arranged by size: 2573 1348 1156 668 592 99 57 BmgBI CAC'GTC Cuts at: 1318 3745 1318 Size: 2427 4066 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2672 4224 5182 Size: 416 53 83 399 24 1255 1552 958 Cuts at: 5182 442 Size: 1753 Fragments arranged by size: 1753 1552 1255 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3285 3642 1056 Size: 2229 357 3907 Fragments arranged by size: 3907 2229 357 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2450 2762 3311 3657 5153 1239 Size: 1211 312 549 346 1496 2579 Fragments arranged by size: 2579 1496 1211 549 346 312 Bpu10I CC'TnA_GC Cuts at: 2896 2896 Size: 6493 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2369 4618 5483 5781 2369 Size: 2249 865 298 3081 Fragments arranged by size: 3081 2249 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5156 882 Size: 617 3657 2219 Fragments arranged by size: 3657 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3581 4284 493 Size: 711 2377 703 2702 Fragments arranged by size: 2702 2377 711 703 BsaBI GATnn'nnATC Cuts at: 3729 6487 3729 Size: 2758 3735 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2062 4300 4682 5317 Size: 53 83 186 936 529 2238 382 635 Cuts at: 5317 275 Size: 1451 Fragments arranged by size: 2238 1451 936 635 529 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2580 4926 5324 5598 5745 6156 2580 Size: 2346 398 274 147 411 2917 Fragments arranged by size: 2917 2346 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6268 Fragments arranged by size: 6268 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2857 1186 Size: 1671 4822 BseYI C'CCAG_C Cuts at: 2097 2284 2816 5696 6192 2097 Size: 187 532 2880 496 2398 Fragments arranged by size: 2880 2398 532 496 187 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3536 1163 Size: 573 1800 4120 Fragments arranged by size: 4120 1800 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2107 2424 3943 4704 5732 1540 Size: 275 186 106 317 1519 761 1028 2301 Fragments arranged by size: 2301 1519 1028 761 317 275 186 106 BsiHKAI G_wGCw'C Cuts at: 2217 2382 3477 3693 4554 4639 5710 2217 Size: 165 1095 216 861 85 1071 3000 Fragments arranged by size: 3000 1095 1071 861 216 165 85 BsmI GAATG_Cn' Cuts at: 2718 3151 4004 4097 2718 Size: 433 853 93 5114 Fragments arranged by size: 5114 853 433 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2211 2217 2382 2439 2538 3206 3477 Size: 399 421 6 165 57 99 668 271 Cuts at: 3477 3693 3812 4554 4639 5710 1391 Size: 216 119 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 668 421 399 271 216 165 119 99 85 57 6 BspEI T'CCGG_A Cuts at: 6156 6156 Size: 6493 BspHI T'CATG_A Cuts at: 4382 6112 4382 Size: 1730 4763 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3848 844 Size: 3004 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2088 2137 2326 4380 1394 Size: 429 265 49 189 2054 3507 Fragments arranged by size: 3507 2054 429 265 189 49 BsrDI GCAATG_nn' Cuts at: 65 1248 2323 4988 5162 65 Size: 1183 1075 2665 174 1396 Fragments arranged by size: 2665 1396 1183 1075 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2308 2423 2580 5137 5324 1700 Size: 110 498 115 157 2557 187 2869 Fragments arranged by size: 2869 2557 498 187 157 115 110 BsrGI T'GTAC_A Cuts at: 96 2830 96 Size: 2734 3759 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6493 BssSI C'ACGA_G Cuts at: 2375 4553 5565 2375 Size: 2178 1012 3303 Fragments arranged by size: 3303 2178 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6493 BstBI TT'CG_AA Cuts at: 5297 5297 Size: 6493 BstEII G'GTnAC_C Cuts at: 1184 2797 1184 Size: 1613 4880 BstYI r'GATC_y Cuts at: 1972 3823 4190 4575 4592 6044 6130 6142 6488 Size: 1851 367 385 17 1452 86 12 346 Cuts at: 6488 1972 Size: 1977 Fragments arranged by size: 1977 1851 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2107 2529 513 Size: 552 939 103 422 4477 Fragments arranged by size: 4477 939 552 422 103 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5754 Fragments arranged by size: 5754 683 56 BtsI GCAGTG_nn' Cuts at: 2501 2807 2988 3007 4009 4803 4823 2501 Size: 306 181 19 1002 794 20 4171 Fragments arranged by size: 4171 1002 794 306 181 20 19 ClaI AT'CG_AT Cuts at: 1554 2127 1554 Size: 573 5920 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6458 DraI TTT'AAA Cuts at: 3797 3797 Size: 6493 DraIII CAC_nnn'GTG Cuts at: 3547 3547 Size: 6493 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5500 809 Size: 507 4184 1802 Fragments arranged by size: 4184 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2104 2421 3940 Size: 54 1425 50 275 18 274 317 1519 Cuts at: 3940 4829 6458 8 Size: 889 1629 43 Fragments arranged by size: 1629 1519 1425 889 317 275 274 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2104 2421 3940 1537 Size: 275 292 317 1519 4090 Fragments arranged by size: 4090 1519 317 292 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2144 2222 3001 4423 1353 Size: 791 78 779 1422 3423 Fragments arranged by size: 3423 1422 791 779 78 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2237 2342 2476 5080 5454 5600 Size: 1064 138 648 105 134 2604 374 146 Cuts at: 5600 387 Size: 1280 Fragments arranged by size: 2604 1280 1064 648 374 146 138 134 105 EcoICRI GAG'CTC Cuts at: 2215 2215 Size: 6493 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2450 2762 3311 3486 3488 3657 4556 Size: 393 818 312 549 175 2 169 899 Cuts at: 4556 5153 5940 6208 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 899 818 787 597 549 393 312 268 175 169 2 EcoNI CCTnn'n_nnAGG Cuts at: 2658 2658 Size: 6493 EcoO109I rG'GnC_Cy Cuts at: 2534 2535 2846 3632 2534 Size: 1 311 786 5395 Fragments arranged by size: 5395 786 311 1 EcoRI G'AATT_C Cuts at: 3802 4180 3802 Size: 378 6115 FseI GG_CCGG'CC Cuts at: 2427 2427 Size: 6493 FspI TGC'GCA Cuts at: 4999 4999 Size: 6493 HaeII r_GCGC'y Cuts at: 1678 2015 2065 2392 2403 3766 5640 1678 Size: 337 50 327 11 1363 1874 2531 Fragments arranged by size: 2531 1874 1363 337 327 50 11 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2588 2620 3308 3340 5134 Size: 32 135 32 1016 32 688 32 1794 Cuts at: 5134 5166 5208 5240 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1794 1016 688 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3837 4083 669 Size: 988 281 1899 246 3079 Fragments arranged by size: 3079 1899 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6493 HpaI GTT'AAC Cuts at: 4083 4083 Size: 6493 KasI G'GCGC_C Cuts at: 2061 2061 Size: 6493 KpnI G_GTAC'C Cuts at: 3818 3818 Size: 6493 MfeI C'AATT_G Cuts at: 3070 4092 3070 Size: 1022 5471 MluI A'CGCG_T Cuts at: 6369 6369 Size: 6493 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2813 2948 3316 5607 5791 1305 Size: 1508 135 368 2291 184 2007 Fragments arranged by size: 2291 2007 1508 368 184 135 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4671 Fragments arranged by size: 4671 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2961 4451 4810 4969 518 Size: 648 1795 1490 359 159 2042 Fragments arranged by size: 2042 1795 1490 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2097 2109 2371 2464 2531 2930 3289 3909 Size: 491 12 262 93 67 399 359 620 Cuts at: 3909 4586 5734 5979 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 620 491 399 359 262 245 93 67 12 NaeI GCC'GGC Cuts at: 2425 2425 Size: 6493 NarI GG'CG_CC Cuts at: 2062 2062 Size: 6493 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5002 Fragments arranged by size: 5002 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6493 NgoMIV G'CCGG_C Cuts at: 2423 2423 Size: 6493 NheI G'CTAG_C Cuts at: 1052 3281 3638 1052 Size: 2229 357 3907 Fragments arranged by size: 3907 2229 357 NotI GC'GGCC_GC Cuts at: 3940 3940 Size: 6493 NsiI A_TGCA'T Cuts at: 2718 2718 Size: 6493 NspI r_CATG'y Cuts at: 1132 1229 2837 3851 5396 1132 Size: 97 1608 1014 1545 2229 Fragments arranged by size: 2229 1608 1545 1014 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6493 PciI A'CATG_T Cuts at: 2833 5392 2833 Size: 2559 3934 PflMI CCAn_nnn'nTGG Cuts at: 1108 2058 2097 1108 Size: 950 39 5504 Fragments arranged by size: 5504 950 39 PfoI T'CCnGG_A Cuts at: 2719 2719 Size: 6493 PmeI GTTT'AAAC Cuts at: 3797 3797 Size: 6493 PmlI CAC'GTG Cuts at: 3581 3581 Size: 6493 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4562 4594 6075 6107 4562 Size: 32 1481 32 4948 Fragments arranged by size: 4948 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2846 2846 Size: 6493 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6493 PsiI TTA'TAA Cuts at: 4063 4063 Size: 6493 PspOMI G'GGCC_C Cuts at: 2534 2534 Size: 6493 PstI C_TGCA'G Cuts at: 830 2508 2814 3083 3442 3845 830 Size: 1678 306 269 359 403 3478 Fragments arranged by size: 3478 1678 403 359 306 269 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6493 PvuII CAG'CTG Cuts at: 2371 2930 3289 2371 Size: 559 359 5575 Fragments arranged by size: 5575 559 359 SacI G_AGCT'C Cuts at: 2217 2217 Size: 6493 SacII CC_GC'GG Cuts at: 2110 2532 2110 Size: 422 6071 SalI G'TCGA_C Cuts at: 1936 3835 1936 Size: 1899 4594 SapI GCTCTTCn'nnn_ Cuts at: 2144 2222 2144 Size: 78 6415 SbfI CC_TGCA'GG Cuts at: 3845 3845 Size: 6493 ScaI AGT'ACT Cuts at: 2647 2906 3085 4741 6453 2647 Size: 259 179 1656 1712 2687 Fragments arranged by size: 2687 1712 1656 259 179 SexAI A'CCwGG_T Cuts at: 3543 3543 Size: 6493 SfcI C'TryA_G Cuts at: 826 1045 2504 2565 2810 2936 3079 3306 3438 Size: 219 1459 61 245 126 143 227 132 Cuts at: 3438 3841 4976 5657 5848 826 Size: 403 1135 681 191 1471 Fragments arranged by size: 1471 1459 1135 681 403 245 227 219 191 143 132 126 61 SfoI GGC'GCC Cuts at: 2063 2063 Size: 6493 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6493 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6493 SmaI CCC'GGG Cuts at: 1872 2167 3820 1872 Size: 295 1653 4545 Fragments arranged by size: 4545 1653 295 SmlI C'TyrA_G Cuts at: 820 1017 1943 2384 4597 5498 5760 820 Size: 197 926 441 2213 901 262 1553 Fragments arranged by size: 2213 1553 926 901 441 262 197 SnaBI TAC'GTA Cuts at: 493 4284 493 Size: 3791 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6493 SphI G_CATG'C Cuts at: 1132 3851 1132 Size: 2719 3774 SrfI GCCC'GGGC Cuts at: 2167 2167 Size: 6493 SspI AAT'ATT Cuts at: 5 52 4417 5 Size: 47 4365 2081 Fragments arranged by size: 4365 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6493 StyI C'CwwG_G Cuts at: 513 1065 2004 2707 3932 513 Size: 552 939 703 1225 3074 Fragments arranged by size: 3074 1225 939 703 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4520 4537 4690 4875 5320 325 Size: 1255 2940 17 153 185 445 1498 Fragments arranged by size: 2940 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2645 2773 2830 Size: 274 80 33 51 1443 668 128 57 Cuts at: 2830 2904 3083 3381 4739 6451 96 Size: 74 179 298 1358 1712 138 Fragments arranged by size: 1712 1443 1358 668 298 274 179 138 128 80 74 57 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6453 6485 6453 Size: 32 6461 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6493 XbaI T'CTAG_A Cuts at: 3829 3829 Size: 6493 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6493 XmaI C'CCGG_G Cuts at: 1870 2165 3818 1870 Size: 295 1653 4545 Fragments arranged by size: 4545 1653 295 XmnI GAAnn'nnTTC Cuts at: 4622 4622 Size: 6493 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4301 276 Size: 53 83 186 936 2767 2468 Fragments arranged by size: 2767 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoRV FalI FspAI NruI PacI PspXI PsrI RsrII SanDI SfiI SwaI XcmI