Restriction map for
pFN21AB8941
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# NO CUT ENZYMES ( 157 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwI AlwNI AocI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BalI BamHI BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BsgI BsiEI BsiWI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstYI BstZ17I Bsu36I
CelII ClaI DraI DraIII DrdI
EaeI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HgaI Hin1I HindIII HinfI HpaI
KasI KpnI Ksp632I KspI MaeII
MamI MfeI MflI MluI MluNI
MroI MscI MslI MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI SacII SapI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI TfiI Tsp509I
Tth111I Van91I XbaI XcmI XhoII
XmaCI XmaI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AccI 858 GTCGAC GT^[AC][GT]AC
Asp700I 18 GAACTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 721 GAGCTC G[AT]GC[AT]^C
BanI 219 GGTGCC G^G[CT][GA]CC
BfaI 358 CTAG C^TAG
BseRI 475 CTCCTC GAGGAG(10/8)
BsiHKAI 721 GAGCTC G[AT]GC[AT]^C
BsmAI 317 GAGAC GTCTC(1/5)
BsmBI 317 GAGACG CGTCTC(1/5)
BspMI 385 GCAGGT ACCTGC(4/8)
CpoI 396 CGGTCCG CG^G[AT]CCG
DpnII 472 GATC ^GATC
DsaI 524 CCACGG C^C[GA][CT]GG
EcoNI 477 CCTCTCCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 827 CCAAGG C^C[AT][AT]GG
FauI 190 CCCGC CCCGC(4/6)
MaeI 358 CTAG C^TAG
MboI 472 GATC ^GATC
MboII 774 GAAGA GAAGA(8/7)
MseI 554 TTAA T^TAA
NdeII 472 GATC ^GATC
NlaIII 863 CATG CATG^
NspI 862 ACATGC [GA]CATG^[CT]
PaeR7I 339 CTCGAG C^TCGAG
PstI 264 CTGCAG CTGCA^G
RsrII 396 CGGTCCG CG^G[AT]CCG
SacI 721 GAGCTC GAGCT^C
SalI 858 GTCGAC G^TCGAC
Sau3AI 472 GATC ^GATC
ScaI 290 AGTACT AGT^ACT
SmlI 339 CTCGAG C^T[CT][GA]AG
StuI 594 AGGCCT AGG^CCT
StyI 827 CCAAGG C^C[AT][AT]GG
Tru9I 554 TTAA T^TAA
Tsp45I 895 GTCAC ^GT[GC]AC
TspRI 609 CAGTG CA[GC]TG[ACGT][ACGT]^
XhoI 339 CTCGAG C^TCGAG
XmnI 18 GAACTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 15 enzymes) #
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ApaI 350 GGGCCC GGGCC^C
689 GGGCCC
AvaI 243 CTCGGG C^[CT]CG[GA]G
339 CTCGAG
BpmI 718 CTGGAG CTGGAG(16/14)
839 CTGGAG
BsmFI 51 GGGAC GGGAC(10/14)
802 GTCCC
BsoBI 243 CTCGGG C^[CT]CG[GA]G
339 CTCGAG
BsrFI 394 GCCGGT [GA]^CCGG[CT]
459 ACCGGC
Cfr10I 394 GCCGGT [GA]^CCGG[CT]
459 ACCGGC
HincII 115 GTTGAC GT[CT]^[GA]AC
858 GTCGAC
HindII 115 GTTGAC GT[CT]^[GA]AC
858 GTCGAC
MaeIII 44 GTAAC ^GT[ACGT]AC
895 GTCAC
MspA1I 640 CAGCTG C[AC]G^C[GT]G
836 CAGCTG
PpuMI 51 GGGACCC [GA]G^G[AT]CC[CT]
800 GGGTCCC
PvuII 640 CAGCTG CAG^CTG
836 CAGCTG
SfaNI 844 GATGC GCATC(5/9)
420 GCATC
SfcI 63 CTACAG C^T[GA][CT]AG
264 CTGCAG
#########################################
# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AccII AciI AfaI AluI AvaII
BanII BbvI BcnI BmyI BsaJI
BsiYI BslI Bsp1286I BsrI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DdeI DraII EcoO109I EcoRII
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HpaII HphI ItaI
MnlI MspI MvaI MvnI MwoI
NciI NlaIV RsaI Sau96I ScrFI
TaqI TseI TthHB8I